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131.
132.
本试验从健康的鸡盲肠内容物中筛选出具有较高抑菌活性的病源拮抗菌JM-11菌株,经形态、生理生化及16S rDNA序列鉴定此菌为枯草芽孢杆菌(Bacillus subtilis subsp. subtilis)。经耐受胃肠道环境及胆盐试验,结果表明,JM-11菌株能耐受0.3%的胆盐环境,在pH为3.0的人工胃液中能够很好的存活。利用抗生素打乱雏鸡肠道菌群后,饲喂致病性大肠杆菌来制作腹泻模型。经益生菌JM-11对腹泻雏鸡的治疗试验结果显示,JM-11能显著降低腹泻雏鸡粪便中的大肠杆菌的数量(P<0.01),增加乳酸菌的数量(P<0.01),提高雏鸡免疫器官指数(P<0.05),对雏鸡具有安全无毒的作用。 相似文献
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134.
对发生临床疑似伪狂犬病毒(PRV)感染的牧羊犬组织样品,进行研磨、细胞接种、传代培养、细胞病变效应(CPE)观察、PCR检测、效价测定、电镜观察、IFA检测、家兔接种以及基因测序分析等,结果成功分离到1株犬源PRV毒株,命名为XJ-14株。该毒株可致PK-15细胞产生变圆、破碎、逐渐呈拉网式漂浮等特征性细胞病变;PRV gE-PCR检测阳性,病毒效价达10~(-7.45)/0.1 mL;病毒粒子在电镜下呈圆形,有囊膜,直径约150 nm;PRV荧光抗体检测阳性,接种家兔后可致家兔奇痒、麻痹死亡。对该毒株gC、gE、TK基因进行全基因测序,发现gE糖蛋白的48、497位各插入了1个D,另有12个氨基酸位点发生点突变,其gC蛋白氨基酸序列64~70位出现了7个氨基酸(AASTPAA)插入。结果表明,此牧羊犬病例是由PRV感染所致。 相似文献
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136.
将不同剂量的Epostanc静脉注射成年公兔,测定注射后1、2、3、24小时血清睾酮、皮质醇水平的变化。结果显示注射Epostanc后公兔血清睾酮水平增加且与剂量相关;低剂量Epostanc显著地降低血清皮质醇水平。 相似文献
137.
Effects of dietary arginine supplementation during gestation and lactation on the performance of lactating primiparous sows and nursing piglets 总被引:4,自引:0,他引:4
A 2 x 2 factorial arrangement of treatments in a randomized block design was used to determine the effects of dietary Arg supplementation during gestation and lactation on the lactation performance of 38 first-parity sows. At 30 d of gestation, pregnant gilts were allotted based on BW to 1 of 2 diets supplemented with 1% L-Arg.HCl or 1.7% L-Ala (isonitrogenous control). After farrowing, sows were further allotted based on BW within previous gestation treatment groups to 1 of 2 lactation diets supplemented with 1% L-Arg.HCl or 1.7% L-Ala (isonitrogenous control). All gestation diets contained 3.1 Mcal/kg and 12.2% CP (as is) and were fed 2 kg/d in 2 equally sized meals, whereas all lactation diets contained 3.2 Mcal/kg and 18.6% CP (as is) and were fed ad libitum. Litter size was standardized to 10 piglets by cross-fostering within 24 h postfarrowing. On a weekly basis, BW and backfat (BF) thickness of sows, as well as piglet BW were measured, and blood and milk samples were obtained from the sows. Number of days from weaning to estrus and ADFI were also recorded. There were no differences in BW, BF thickness, ADFI, or days until return to estrus among treatment groups. There was no effect of the gestation diet or a gestation x lactation diet interaction on any parameter measured. On d 7 of lactation, plasma concentrations of Arg and insulin in sows, as well as concentrations of most AA in milk, were greater (P < 0.05) in response to Arg supplementation during lactation compared with the control. Weight gain of piglets from sows fed the Arg-supplemented diet during lactation was greater between d 0 and 7 (P < 0.01) and between d 0 and 21 (P < 0.05) of lactation compared with piglets from sows fed the control diet. Collectively, results from this study indicate the potential beneficial effects of dietary Arg supplementation in improving the lactation performance of first-parity sows. 相似文献
138.
1. The present study was conducted to investigate the effects of inulin on laying hens. A total of 360 Brown Nick laying hens were divided randomly into 6 groups of 60 with 6 replicates of 10 hens and fed on diets containing 0 (control), 0·1, 0·5, 1·0, 1·5 or 2·0% inulin during the 4-week trial. 2. Dietary supplementation of inulin reduced cholesterol concentration (mg/g yolk) and content (mg/egg) in eggs. Cholesterol content in eggs decreased linearly with increasing levels of dietary inulin level. 3. Supplementation of inulin in diets decreased coliform bacteria counts and pH in the caecum. The lowest coliform bacteria counts (6·30 ± 0·03 log10 cfu/g) and pH (6·47 ± 0·01) were obtained in the 2·0% inulin group, the two indices decreasing by 21·6% and 3·0% respectively, compared with the control group. Coliform bacteria count and pH were changed linearly in accordance with increasing levels of dietary inulin level. Caecal Bifidobacteria counts were increased in the 2·0%-inulin group. 4. Inulin supplementation of layer diets did not appear to have any adverse effects on laying rate, egg weight, feed intake, feed conversion efficiency, cracked-egg rate, eggshell thickness or Haugh unit compared with the control laying hens. 5. Therefore, dietary supplementation with inulin may lead to the development of low-cholesterol chicken eggs as demanded by health-conscious consumers. 相似文献
139.
140.
按照全国草种区划的部署,华南区包括闽,粤、桂、琼四省(区)和云南省的南部地区。总结和分析五十年代以后华南各省(区)有关单位在牧草引种驯化和筛选中取得的成果和经验。对华南的自然环境,气候分区和土壤类型,主要草种的筛选依据及各草种的特性、适应性和生产性能进行了深入研究。结合主要草种小区补充重复试验结果和生产调查,所有结果都显示适应华南种植的是热带栽培牧草。只有北和西北部边缘中山地区(海拔600—800米以上)适宜种植温带栽培草种。经综合评价再按照作者提出的分区原则、指标和亚区分区方法,华南区的主要多年生栽培草种可以划分为四个亚区:①闽、粤、桂南部和海南全部(热带和南亚热带)丘陵平原大翼豆、银合欢、格米姆柱花草、卡松古鲁狗尾草、宽叶雀稗和象草亚区;②闽、粤、桂北部(中亚热带)低山丘陵绿叶山蚂蝗,银叶山蚂蝗、银合欢、宽叶雀稗和小花毛花雀稗亚区;③闽、粤、桂北部和西北部中山(中亚热带山地)白三叶、多年生黑麦草和鸭茅亚区;④滇南(热带和南亚热带)低山丘陵大翼豆、格拉姆柱花草、宽叶雀稗和象草亚区。对各亚区的自然概况和草坡特点作了简述。 相似文献