全文获取类型
收费全文 | 5279篇 |
免费 | 315篇 |
国内免费 | 689篇 |
专业分类
林业 | 507篇 |
农学 | 668篇 |
基础科学 | 420篇 |
801篇 | |
综合类 | 2020篇 |
农作物 | 374篇 |
水产渔业 | 265篇 |
畜牧兽医 | 659篇 |
园艺 | 201篇 |
植物保护 | 368篇 |
出版年
2024年 | 20篇 |
2023年 | 90篇 |
2022年 | 212篇 |
2021年 | 253篇 |
2020年 | 248篇 |
2019年 | 232篇 |
2018年 | 191篇 |
2017年 | 229篇 |
2016年 | 184篇 |
2015年 | 261篇 |
2014年 | 269篇 |
2013年 | 319篇 |
2012年 | 385篇 |
2011年 | 340篇 |
2010年 | 375篇 |
2009年 | 298篇 |
2008年 | 292篇 |
2007年 | 266篇 |
2006年 | 216篇 |
2005年 | 239篇 |
2004年 | 144篇 |
2003年 | 155篇 |
2002年 | 204篇 |
2001年 | 172篇 |
2000年 | 156篇 |
1999年 | 94篇 |
1998年 | 65篇 |
1997年 | 52篇 |
1996年 | 48篇 |
1995年 | 59篇 |
1994年 | 40篇 |
1993年 | 40篇 |
1992年 | 27篇 |
1991年 | 25篇 |
1990年 | 26篇 |
1989年 | 25篇 |
1988年 | 10篇 |
1987年 | 9篇 |
1986年 | 2篇 |
1985年 | 5篇 |
1984年 | 1篇 |
1982年 | 3篇 |
1981年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有6283条查询结果,搜索用时 15 毫秒
21.
YU Lei HAN Bing TIAN Tian ZHENG Lu YANG Ting LIU Xing TANG Lei LUO Xuan YANG Qin XIE Ru-jia 《园艺学报》2017,33(12):2151-2156
AIM: To investigate the effect of suberoylanilide hydroxamic acid (SAHA) on the proliferation and apoptosis of human hepatocellular carcinoma HepG2 cells and to explore its possible mechanism. METHODS: HepG2 cells were treated with SAHA at different concentrations for 48 h. The proliferation of HepG2 cells was detected by real-time cellular analysis. The protein levels of acetylated histones H3K9 and H3K27, glucose-regulated protein 78 (GRP78), protein kinase R-like endoplasmic reticulum kinase (PERK) and p-PERK were determined by Western blot. The cell apoptosis was analyzed by flow cytometry. RESULTS: Compared with control group, treatment with SAHA at 0.1 μmol/L and 1 μmol/L for 48 h showed no significant inhibitory effect on the proliferation of HepG2 cells, while SAHA at 6 μmol/L and 12 μmol/L significantly inhibited the proliferation of HepG2 cells (P<0.05). The results of Western blot showed that the protein levels of acH3K9, acH3K27, GRP78 and p-PERK increased significantly after treated with SAHA at diffe-rent concentrations for 48 h, while the protein level of PERK was decreased significantly (P<0.05). The results of flow cytometry analysis showed that the apoptotic rates of the HepG2 cells increased with the increase in SAHA concentration. CONCLUSION: SAHA up-regulates the acetylation of H3K9 and H3K27 in the HepG2 cells and induces apoptosis of HepG2 cells by activating the endoplasmic reticulum stress-related apoptosis pathway. 相似文献
22.
拮抗茄子黄萎病菌土壤放线菌的分离筛选和鉴定 总被引:2,自引:1,他引:2
为了研究土壤拮抗性放线菌对茄子黄萎病的生防价值,从陕西省延安市宝塔区与汉中地区土壤中分离、纯化获得154株具有纤维素降解活性的放线菌。采用平板对峙培养法进行初筛,用生长速率法对抑菌效果较好的菌株ZX-10-4进行抑菌谱的测定,并进行盆栽试验验证其对茄子黄萎病的防治效果,通过形态、培养特征及16S rDNA序列分析研究其分类地位。结果显示,有17株放线菌对茄子黄萎病菌抑菌效果较好且抑菌活性稳定,其中菌株ZX-10-4的抑菌活性最高,抑菌率达90.15%;活性菌株ZX-10-4发酵原液对茄子黄萎病的保护和治疗作用分别为70.00%和60.33%,稀释5倍液的防效也在50%以上。根据形态、培养特征、生理生化指标和16S rDNA序列分析,初步确定菌株ZX-10-4为白网链霉菌Streptomyces albireticuli的近似种。 相似文献
23.
25.
26.
27.
Xing Li Xiaodong Wang Zhanhai Kang Zhikuan Ren Weishuai Bi Wenxiang Yang Daqun Liu 《European journal of plant pathology / European Foundation for Plant Pathology》2018,152(2):479-492
Wheat leaf rust caused by Puccinia triticina (Pt) is one of the most severe fungal diseases threatening the global wheat production. The use of leaf rust resistance (Lr) genes in wheat breeding programs is the major solution to solve this issue. Wheat isogenic line carrying the Lr39/41 gene has shown a moderate to high resistance to most of the Pt pathotypes detected in China. In the present study, a typical hypersensitive response (HR) was observed using microscopy in leaves of the Lr39/41 isogenic line inoculated with the avirulent Pt pathotype THTT from 48 h-post inoculation. Two Lr39/41 resistance-associated suppression subtractive hybridization (SSH) libraries with a total of 6000 clones were established. Microarray hybridizations were performed on all obtained SSH clones using RNAs extracted from leaves of the Pt-inoculated and non-inoculated Lr39/41 isogenic lines, and leaves of the Pt-inoculated and non-inoculated Thatcher susceptible lines. Differentially expressed clones were analyzed by significance analysis of microarrays (SAM), followed by further sequencing. A total of 36 Lr39/41-resistance-related differentially expressed genes (DEGs) were identified, many of which had been previously reported to be involved in the plant defense response. The expression levels of eight selected DEGs during different stages of the Lr39/41-mediated resistance were further quantified by a qRT-PCR assay. Several pathogenesis-related (PR) and HR-related genes seem to be crucial for the Lr39/41-mediated resistance. In general, a brief profile of DEGs associated with the Lr39/41-mediated wheat resistance to Pt was drafted. 相似文献
28.
29.
表达马立克氏病病毒CVI988/Rispens株糖蛋白B基因的重组鸡痘病毒疫苗的免疫保护研究 总被引:1,自引:0,他引:1
用鸡痘病毒载体表达马立克氏病病毒(MDV)糖蛋白B(gB)基因构建成重组鸡痘病毒(rFPV)。以MDV GA或Md5和RBIB混合攻毒,在不同品种的鸡中评价rFPV-gB/R单价苗和与火鸡疱疹病毒(HVT)组成的二价苗的免疫保护效力。试验结果表明,MDV疫苗和FPV母源抗体阴性的SPF鸡和母源抗体阳性的商品鸡中,rFDV-gB/R均能提供免疫保护作用,且其中一种商品蛋鸡中rFPV-gB/R与HVT液氮苗或HVT冻干苗结合使用,均有显著的免疫协同保护作用。免疫学研究指出,rFPV-gB/R与常规疫苗一样,可显著地降低疫苗免疫攻毒鸡的病毒血症和羽囊排毒。 相似文献
30.
在崂山茶区采用网捕法和植株拍打法研究了种草和清耕茶园节肢动物数量的变化规律。结果表明,种植紫花苜蓿的第2年,节肢动物数量增加明显,种群结构趋于稳定。共调查到节肢动物12目,44科,81种,类群最大的为蜘蛛目,有27种,其次是同翅目,有12种,鞘翅目有10种,膜翅目有9种,其他23种。种草园与清耕园相比,节肢动物种类增加18.0%~19.0%,害虫数量平均减少75.6%,天敌种类增加10.3%~17.1%,个体数增加64.0%~77.1%,节肢动物数量在一年中的变化幅度平缓。茶园使用化学农药,是影响节肢动物数量的主要因素。在种植紫花苜蓿的茶园,不使用任何化学农药也不致造成害虫危害。 相似文献