Morphological and molecular analysis of genetic variability within isolates of <Emphasis Type="Italic">Corynespora cassiicola</Emphasis> from different hosts

Twenty-two isolates of Corynespora cassiicola obtained from cucumber, papaya, eggplant, tomato, bean, Vigna, sesame and Hevea rubber (Hevea brasiliensis) were analysed by morphological features, the differences of the ribosomal DNA internal transcribed spacer (rDNA-ITS) region sequence and the inter simple sequence repeat (ISSR) technique. Variability of morphological features was observed among the isolates. Pathogenicity tests showed that isolates from different hosts attacked Hevea rubber. Sequences of two outgroup taxa, C. proliferata and C. citricola, were downloaded from GenBank. The phylogenetic trees were constructed by using the rDNA-ITS region sequences from 24 Corynespora spp. isolates. In this analysis, the 24 sequences grouped into two clusters (A and B). Cluster A consists of sequences from all isolates of C. cassiicola; whereas cluster B consists of the two outgroup taxa, C. proliferata and C. citricola. However, the ITS region is conservative, and is not fit for studying differences among isolates. A total of 114 DNA fragments was amplified with 16 ISSR primers, among which 102 were polymorphic (89.5%). A dendrogram was created by the unweighted pair-group method with arithmetic averaging (UPGMA) analysis, and 22 isolates grouped into three clusters (C, D and E). Cluster C is composed of all of the Hevea rubber isolates, whereas cluster D is composed of nine isolates: four from papaya, five from cucumber, eggplant, bean, vigna and sesame. Cluster E is composed of two isolates from cucumber and tomato. These analyses showed that the genetic diversity was very rich among the tested isolates. There are no correlations between the morphological characteristics or rDNA-ITS region sequences of the 22 isolates and their host or geographical origin, but there is a link between ISSR clusters and their host origins. ISSR markers appear to be useful for intra-species population study in C. cassiicola.     

甜玉米小斑病抗性的遗传分析与主效QTL定位

为培育抗病品种,利用抗小斑病甜玉米自交系T14和感小斑病自交系T18为亲本配制杂交组合,对玉米抗小斑病性状进行遗传分析和抗病基因分子标记定位,用主基因＋多基因混合遗传模型和P₁、P₂、F₁、B₁、B₂、F₂ 6世代联合分析的方法对单位叶面积病斑数量进行遗传分析,并应用复合区间作图法检测抗小斑病QTL。结果表明,单位叶面积病斑数量受2对加性-显性-上位性主基因控制,自交系T14的抗病性在各个分离世代都以主基因遗传为主。在第4染色体上检测到4个相互连锁的小斑病抗性QTL,解释表型变异的7.7%、30.9%、14.8%和11.5%;在第6染色体上定位了1个抗病QTL,可解释表型变异的37.7%。检测到的小斑病抗性主效QTL位于第4和第6染色体的特征与2对主基因的遗传模型相吻合。     

油菜小麦邻作模式对麦蚜主要天敌种群动态以及小麦生产的影响*

麦蚜是危害我国小麦生产的重要害虫。本试验探索了油菜小麦邻作模式以及小麦单作模式对麦蚜主要天敌发生的影响,以小麦和油菜交界处向两边各在0、2、4、6、8、16 m处设立试验小区,记录麦蚜主要天敌发生数量以及测定小麦株高、穗长、穗重、千粒重,揭示油菜小麦邻作模式对小麦生产的影响。结果表明：随着油菜的成熟,瓢虫逐渐向油菜小麦邻近处转移,且迁移的最有效距离为4 m;对邻作小麦田蚜茧蜂数量最高峰当日数据进行分析,发现除最高的0 m（2009年）和4 m（2010年）处外,其他各距离之间不存在显著差异,而单作田各距离之间蚜茧蜂数量上的差异不明显;相比于小麦单作模式,油菜小麦邻作显著提高了小麦的株高、穗长、穗重、千粒重。总之,油菜小麦邻作模式有利于麦蚜主要天敌向小麦田转移,并能提高小麦的产量和生物量。     

Induction effects of host plants on insecticide susceptibility and detoxification enzymes of Bemisia tabaci (Hemiptera: Aleyrodidae)

BACKGROUND: The polyphagous B‐biotype Bemisia tabaci (Gennadius) has developed a high resistance to commonly used insecticides in China. To illustrate the induced changes by host plant, bioassay and biochemical research on five different host populations were investigated. RESULTS: Except for bifenthrin, all tested insecticides showed lower toxicity to the B. tabaci poinsettia population compared with other host populations. Moreover, four insecticides, the exceptions being abamectin and fipronil, showed highest toxicity towards the tomato population. The LC₅₀ values of the poinsettia population, particularly towards acetamiprid, were 14.8‐, 10.3‐ and 7.29‐fold higher than those of tomato, cucumber and cabbage respectively. The CarE activities of B. tabaci cabbage and cucumber populations were all significantly higher than those of poinsettia, cotton and tomato populations. The ratio of the cabbage population was 1.97‐, 1.79‐ and 1.30‐fold higher than that of poinsettia, cotton and tomato respectively. The frequency profiles for this activity also have obvious differences. The GST and P450 activities of the cucumber population were the lowest in the five host populations. CONCLUSION: Long‐term induction of host plants for B‐biotype B. tabaci could influence their susceptibilities to several insecticides. Rational selection and usage of insecticides for particular hosts will be helpful for resistance management and control of this species. Copyright © 2010 Society of Chemical Industry     

施肥对地被菊生长发育及开花的影响

对施肥后的地被菊花营养含量及水分含量的测定表现，N、P在植株的生和或期需求最较大，K肥元素随着植株的生长其需求量逐渐下降，对水分的需求基本上是：生长中期＞生长初期＞生长末期，说明生长中期地被菊新陈代谢最旺盛，生长末期水分含量逐渐降低，是干物质积累的过程，合理施肥不仅可以促进地被菊的生长，提高其观赏性，而且提前花期3－7d.     

新型植物生长调节剂吡啶脲的合成和鉴定

报道了Ｎ－苯基－Ｎ＇－（４－吡啶基）脲的合成与反应条件，认为合成该物质的操作简便，而且产率较高．对产物进行了红外光谱、核磁共振谱和元素分析等鉴定     

两步厌氧消化器处理豆制品

本文报道了用两种不同类型的两步厌氧消化器(同心圆固定膜和分离式两步厌氧消化器)处理豆制品废水的初步研究结果。同心圆固定膜和分离式两步厌氧消化器分别达到了3.0L/L、d和4.9L/L、d的产生率、5.7gCOD/L、d和10.7gCOD/L、d的有机物负荷率、38小时和28小时的水滞留期,90％左右的COD去除率,相似的甲烷含量,能保持正常运转的pH值和两种消化器均不同程度地形成颗粒污泥的运转效果。分离式两步厌氧消化器的酸化反应器达到了83gCOD/L、d的COD负荷率,3.4小时的水滞留期、保持4.8—5.0的pH值、较高的挥发酸含量的酸化效果。以上数据表明,分离式两步厌氧消化器具有较理想的处理豆制品废水效果。     

养鸡场的地热降温系统研究

针对重庆地区的气候特点，提出将地热降温应用于养鸡场，并采用模糊控制技术对鸡舍的温度进行自动控制，旨在降低养鸡场的生产成本，提高其效益，实践证明可行。     

格氏栲天然林与人工林土壤异养呼吸特性及动态

通过用静态碱吸收法对中亚热带福建三明格氏栲自然保护区内的格氏栲天然林和33年生的格氏栲人工林及杉木人工林的土壤异养呼吸进行为期2年的定位研究。结果表明，三种森林枯枝落叶层呼吸和无根土壤呼吸速率季节变化均呈单峰曲线，最大峰值出现在5月至6月，最小值出现在12月至1月。格氏栲天然林、格氏栲人工林和杉木人工林枯枝落叶层呼吸速率平均值分别为CO2 79．88、44．37和21．02mgm^-2h^-1，无根土壤呼吸速率平均值分别为CO2 217．4、85．85和94．04mg m^-2h^-1。2002年枯枝落叶层呼吸速率和无根土壤呼吸速率主要受土壤温度影响，但在极端干旱的2003年则主要受土壤湿度的影响。双因素关系模型（R=ae^bTW^c）拟合结果优于仅考虑土壤温度或土壤湿度的单因素关系模型，土壤温度和土壤湿度共同解释不同年份枯枝落叶层呼吸和无根土壤呼吸速率季节变化的82％～85％和85％～92％。不同森林枯枝落叶层呼吸对土壤温度和湿度的敏感性均高于无根土壤呼吸的。格氏栲天然林、格氏栲人工林和杉木人工林枯枝落叶层呼吸年通量分别为C3．76、2．63和1．23t hm^-2a^-1，无根土壤呼吸年通量则分别为C3．44、2．79和1．49t hm^-2a^-1。不同森林土壤异养呼吸通量的差异主要与枯落物数量和质量、土壤有机质数量和质量的差异有关。杉木林枯枝落叶层呼吸对干旱敏感性高于格氏栲（天然林和人工林）的，而人工林（杉木和格氏栲）的土壤有机C对干旱敏感性则要高于格氏栲天然林。     

皆伐对杉木人工林土壤呼吸的影响

应用密闭室碱吸收法对杉木人工林皆伐后的土壤呼吸及各分室呼吸进行为期1年定位研究,结果表明,杉木林皆伐后前4个月土壤呼吸显著高于对照(未伐地)的,皆伐6个月后则显著低于对照的,但伐后1年内的平均土壤呼吸则与对照的无显著差异。皆伐地枯枝落叶层呼吸和矿质土壤呼吸分别在伐后的5个月和6个月内显著高于对照的,但此后则与对照的无显著差异。皆伐地根系呼吸除在伐后当月显著高于对照的外,第3个月迅速降低至消失。皆伐地土壤呼吸、枯枝落叶层呼吸和矿质土壤呼吸最大值出现时间均较对照的有所提前。伐后1年内皆伐地枯枝落叶层呼吸、矿质土壤呼吸和根系呼吸占土壤呼吸的比例分别为34·5%、63·9%和1·6%,而对照的则分别为23·4%、50·1%和26·5%。双因素关系模型拟合结果表明,土壤温度和土壤湿度共同解释皆伐和对照土壤呼吸速率变化的54%和90%。皆伐地土壤呼吸及各分室呼吸对土壤温度的敏感性低于对照的,但对土壤湿度的敏感性则高于对照的。皆伐地土壤呼吸、矿质土壤呼吸和枯枝落叶层呼吸的Q10分别为1·42、1·53和1·34,而对照的土壤呼吸、矿质土壤呼吸、枯枝落叶层呼吸和根系呼吸的Q10则分别为2·42、1·81、2·40和4·41。