农村水资源管理与可持续发展对策研究

分析了我国农村水资源使用存在的主要问题,在此基础上,从可持续发展角度提出构建农村水资源管理体系的对策.     

汕优63在不同海拔高度种植的生育特点及高产栽培技术

汕优６３是浙江省临安县单季杂交稻当家组合．由于受不同海拔高度温光条件限制，一般限于海拔４００米以下地区种植。在适栽范围，应针对不同海拔高度气温和生育期变化，采取相应栽培技术措施．才能兴利避害，获得丰收。     

优质晚籼稻产量因素分析及育种应用探讨

对62份优质晚籼稻新品系产量因素进行相关分析和通径分析．结果表明：各产量因素与产量均呈正相关,其中穴穗数、结实率和穗长与产量的相关达极显著或显著水平;对产量的净贡献率大小依次为穴穗数(39．2％)、结实率(24．58％)、每穗总粒数(17．8％)、千粒重(15．37％)。株高和穗长作用极微弱且为负值。各产量因素问相互制约,相互促进。根据研究结果和育种实践,认为优质晚籼稻高产育种的主攻方向是提高穴穗数、结实率和总粒数;高产(产量在550kg／667m^2以上)结构模式应该是有效穗21—23万穗／667m^2,每穗总粒数160—175粒,结实率85％-90％,千粒重25—28g.     

泉州市发展城市林业的思考

从发展城市林业的意义和泉州市城市林业建设现状,分析了城市林业建设存在的主要问题,提出了泉州市发展城市林业的建议。     

The use of T‐DNA tagging to isolate mutants of Colletotrichum gloeosporioides and Colletotrichum acutatum with reduced virulence against Hevea brasiliensis

Agrobacterium tumefaciens‐mediated transformation (ATMT) is being increasingly recognized as an effective insertional mutagenesis tool in studies of filamentous fungi for gene discovery and functional analysis. We developed and optimized ATMT for 2 Colletotrichum species, Colletotrichum gloeosporioides and Colletotrichum acutatum, the causative agents of Colletotrichum leaf disease in rubber trees in Southern China. A. tumefaciens strain AGL‐1 carrying an ILV1 gene and a green fluorescent protein gene were used to transform the conidia of these 2 Colletotrichum species. The transformation efficiency was correlated with the co‐cultivation duration and bacterial cell concentrations, which reached 300–400 transformants per 1 × 10⁶ conidia after optimization. Southern blot analysis indicated that about 60.0% of the C. gloeosporioides transformants and 46.2% of the C. acutatum transformants had a single copy of T‐DNA in their genomes. Fungal genomic DNA segments flanking the T‐DNA were identified in the transformants through thermal asymmetrical interlaced polymerase chain reaction followed by sequencing. The flanking sequences from 4 C. acutatum and 7 C. gloeosporioides transformants showed moderate or weak homology to the NCBI database entries. Some sequences matching those reported virulence‐related genes. The results suggest that the T‐DNA inserted mutants banks constructed are useful for the discovery of new or important genes and to elucidate their function in the 2 Colletotrichum species.     

别墅的环境景观设计浅谈

如何对别墅的外部环境空间进行设计逐渐形成一个课题,基于多种的设计影响因素进行梳理并提出理念的总结。     

红掌组织培养和快速繁殖技术

就红掌的组培快繁技术进行了研究.结果表明:①红掌组织培养较适合的外植体是腋芽和嫩叶片;②适合愈伤组织诱导的培养基是MS+BA 1.0+2,4-D 0.1+NAA 0.1,有利于愈伤组织增殖及不定芽诱导的培养基是1/2MS+BA 0.5+CM 10%,诱导生根的适宜培养基是1/2MS+IBA2.0.     

Classification research and types of slow controlled release fertilizers (SRFs) used - a review

Slow controlled release fertilizer (SRF) is the hot direction of new fertilizer development, but there is no unified, perfect, standardized classification basis. This paper documents a review of literature related to the classification of SRF, summarizes the history about development for the classification of SRF in three phases, proposes an improved classification of physical, chemical, and compound types. The aim of the current review is to provide evidence on which to base the selection and further development of innovative enhanced efficiency SRF. The study also further clarifies the attribution of various fertilizers and illustrates the use of representative fertilizers in each category. In addition, the compound type of SRF is given the positive perspectives.     

Enzymatically catalyzed synthesis of low-calorie structured lipid in a solvent-free system: optimization by response surface methodology

A kind of low-calorie structured lipid (LCSL) was obtained by interesterification of tributyrin (TB) and methyl stearate (St-ME), catalyzed by a commercially immobilized 1,3-specific lipase, Lipozyme RM IM from Rhizomucor miehei . The condition optimization of the process was conducted by using response surface methodology (RSM). The optimal conditions for highest conversion of St-ME and lowest content LLL-TAG (SSS and SSP; S, stearic acid; P, palmitic acid) were determined to be a reaction time 6.52 h, a substrate molar ratio (St-ME:TB) of 1.77:1, and an enzyme amount of 10.34% at a reaction temperature of 65 °C; under these conditions, the actually measured conversion of St-ME and content of LLL-TAG were 78.47 and 4.89% respectively, in good agreement with predicted values. The target product under optimal conditions after short-range molecular distillation showed solid fat content (SFC) values similar to those of cocoa butter substitutes (CBS), cocoa butter equivalent (CBE), and cocoa butters (CB), indicating its application for inclusion with other fats as cocoa butter substitutes.     

Inhibitory effect of phenolic acids on the proliferation of 3T3-L1 preadipocytes in relation to their antioxidant activity

Obesity is an important topic in the world of public health and preventive medicine. Inhibition of preadipocyte proliferation plays an important role in the mechanisms of proposed antiobesity. In this in vitro study, the inhibitory effect of phenolic acids on 3T3-L1 preadipocytes was evaluated, and a relationship analysis was then conducted. The results showed that the addition of phenolic acids to the growth medium decreased the cell population growth of 3T3-L1 preadipocytes. The IC50 values of chlorogenic acid, gallic acid, o-coumaric acid and m-coumaric acid on 3T3-L1 preadipocytes were 72.3, 43.3, 48.2, and 49.2 microM, respectively. A relationship analysis indicated that there is a significant linear correlation between the influence of phenolic acids on cell population growth and their antioxidant activity (r = 0.77, p < 0.01). The cell cycle assay indicated that the treatment of 3T3-L1 preadipocytes with chlorogenic acid, o-coumaric acid, and m-coumaric acid caused cell cycle arrest in the G1 phase. Gallic acid did not affect the cell cycle profile; however, it increased the number of apoptotic cells (sub-G1 phase) in a time- and dose-dependent manner. Annexin V-fluorescein isothiocyanate (FITC)-propidium iodide (PI) apoptosis flow cytometric assay showed that gallic acid increased the number of early apoptotic (annexin V-FITC+/PI-) and late apoptotic cells (annexin V-FITC+/PI+) but not necrotic cells (annexin V-FITC-/PI+). The treatment of cells with gallic acid caused the loss of mitochondrial membrane potential (delta psi(m)). These results indicate that the inhibition of preadipocyte population growth by some phenolic acids might have further implication in in vivo antiobesity effects.