泛素/26S蛋白酶体途径调节非生物胁迫的研究进展

随着环境的恶化和资源的匮乏,非生物胁迫已成为制约植物生长和发育的重要因素,严重影响农作物的产量和农产品的品质。泛素/26S蛋白酶体途径(ubiquitin/26Sproteasome pathway,UPP)通过特异性地降解泛素化修饰的蛋白质,介导了植物生长发育的诸多方面,并在植物应对非生物胁迫过程中起关键的调控作用。该文主要概述了泛素/26S蛋白酶体途径及其调控植物响应非生物胁迫机制的研究进展。     

七星关区现推脱毒马铃薯品比试验研究

马铃薯是粮、饲、菜兼用作物,也是毕节市主栽夏收作物之一,随着种植业结构的调整,产品深加工和农业产业化的发展,马铃薯价值逐渐被重视。为了改变毕节市马铃薯耕作管理粗放,生产技术落后,品种单一、退化、单产低的现状,配合2010~2011年度马铃薯高产创建项目实施,特设立脱毒马铃薯新品种比较试验,以期从中筛选出适宜毕节市气候条件种植的脱毒马铃薯新品种,为毕节市大面积生产优质马铃薯提供依据。     

基于模糊评判法的长安区滑坡崩塌灾害易发性分区评价

在对长安区滑坡崩塌地质灾害发育现状及形成条件分析的基础上,结合地质灾害易发性分区评价方法,采用模糊综合评判法对长安区滑坡崩塌地质灾害进行了易发性区划评价。结果表明,长安区滑坡崩塌易发性分区分为6个高易发区域、3个中易发区、1个低易发区(C)和1个不易发区(D),高易发区总面积为190.94 km2,中易发区为382.93 km2,低易发区为299.21 km2,不易发区为707.56km2。     

新型国产白油佐剂与进口Marcol-52白油佐剂配制疫苗的比较试验

The Newcastle disease inactivated vaccines were prepared with oil adjuvants respectively from domestic and import.The experiments were done in order to compare the new domestic oil and Marcol-52 according to a series of testing indexes. The results showed that the quality of the new domestic oil was better than Marcol-52, except the absorptive quality.     

超声波辅助法提取凤尾草中黄酮类化合物的影响因素研究

采用超声波辅助法提取了凤尾草中的黄酮类化合物,研究了影响凤尾草黄酮类化合物提取的多种因素.试验结果表明,在提取温度为40℃,提取时间为40 min且用60%(体积分数,下同)甲醇作为提取剂时提取率最高.     

保定地区几个早熟苹果品种(系)生长观察

为筛选适宜保定地区栽植的早熟苹果品种,以嫁接在SH40中间砧上的不同早熟苹果品种(系)为试材,测定了不同品种(系)早熟苹果树体生长发育指标和果实品质。结果表明:除嘎啦早熟变异外,其他供试品种(系)树体生长较好,3年生时Xu2-7、Xu3-2树高330.0~352.5 cm,4年生时为394.0~417.5 cm,Xu3-2、藤牧1号、信浓红4年生为336.5~381.0 cm。4年生时,干径以Xu2-5最大;短枝、中枝、叶丛枝均以Xu2-5最多,长枝以Xu2-7最多,总枝量以Xu2-5最多;藤牧1号短枝所占比例最高(57.4%)。应用模糊综合评判法得出Xu2-5、Xu2-7、信浓红、藤牧1号果实品质较高。     

Effect of AIR on Inflammatory Reaction Infected by Brucella

In order to investigate the effect of AIR on inflammatory reaction infected by Brucellamelitensis (16M), the AIR domain of Tecpr1 gene of murine macrophages RAW264.7 were knocked down (I-A), overexpressed (O-A) and reversed (OA-IA). Using the chlorine fluorescein (DCFH-DA) as a probe, we detected the variation of ROS production and mitochondria distribution by confocal laser scanning microscopy. We observed the expression changes of NLRP3, ASC and Caspase-1 by qRT-PCR and the expression changes of IL-18,IL-1β and Caspase-1 in host cells by ELISA. The results showed that 16M could stimulate RAW264.7 cells to produce ROS by time-dependent pathway, and I-A group and O-A group showed more abnormal accumulation of mitochondrial. The results of qRT-PCR and ELISA suggested that it had effect on the expression levels of NLRP3, ASC,Caspase-1 and IL-18, IL-1β and Caspase-1 in cells of different groups. Those results indicated that with AIR gene deletion, the release amount of ROS changed, mitochondrial clustered abnormally, and AIR was closely related to the activation of inflammasomes and induction of inflammatory reactions.     

Advances on Detection Methods for Residues of Nitrofurans in Food

Nitrofurans are broad-spectrum antibiotics which have been widely used in food animals to the prevention and treatment of gastrointestinal infections.Due to abuse of drugs by criminals,animals could produce drug-resistant strains,which reduces the role of antibiotics.Meanwhile,excessive drugs residues in food has carcinogenic,teratogenic and mutagenic effects to the human body.In this paper,the damage and the detection methods of the residues of the nitrofurans in food are surveyed.The main detection methods include high performance liquid chromatography,liquid chromatography-mass spectrometry,liquid chromatography tandem mass spectrometry,enzyme-linked immunoassay,colloidal gold immunoassay and fluorescence immunity analysis method.The research status of various detection methods are summarized and the suggestions for further research are put forward.It is expected to provide reference for future research.     

浅析乌兰察布地区金叶榆嫁接以及嫁后管理技术

金叶榆叶片金黄色,枝条密生,树冠丰满,生长旺盛,适合做多种造型,在园林绿化上应用较多;再者,抗寒、抗旱能力强,很适合在乌兰察布地区推广应用,为了满足绿化的需要,对该地区嫁接技术以及嫁后管理技术进行了总结,为当地金叶榆嫁接技术以及嫁后管理技术提供理论参考。     

甘蔗黄叶病毒与花叶病毒CP基因RNAi载体构建与转化甘蔗研究

甘蔗黄叶病和甘蔗花叶病是我国甘蔗最主要的病毒病,感病品种产量下降和品质变劣,传统方法难以防治。RNA干扰技术使培育抗病毒甘蔗品种成为可能。本研究根据甘蔗黄叶病毒和侵染甘蔗的高粱花叶病毒结构与功能特性,广泛收集不同来源病毒分离物CP基因序列,经过比对选取保守序列作为干扰序列。通过在序列两端添加酶切位点,合成并连接成双价甘蔗黄叶病和花叶病毒干扰序列,然后构建到中间载体p HANNIBAL上,形成双价RNAi干扰结构,最后插入到p ART27上形成干扰表达载体。利用基因枪轰击甘蔗品种福农15号愈伤组织进行转化,经G418筛选获得抗性再生植株。通过提取DNA和RNA分析,证实双价RNAi干扰结构不仅以不同拷贝整合到甘蔗基因组中,而且得到了转录表达。