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81.
To establish a rapid,sensitive and specific assay for the differential detection of Nipah virus (NiV) and highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV),a duplex Real-time RT-PCR was developed with specific primers and probes targeting to the special sequences of NiV M gene and HP-PRRSV nsp2 gene by optimization of reaction conditions.The performance of the assay was linear ranging from 4.6×101 to 4.6×107 copies/μL for RNA standard control of NiV M (NiV-M-RNA) and from 4.1×101 to 4.1×108 copies/μL for RNA standard control of HP-PRRSV nsp2 (HP-PRRSV-nsp2-RNA),and detection limits of the assay was 46 copies for the NiV-M-RNA and 4.1 copies for the HP-PRRSV-nsp2-RNA,respectively.The coefficients of variation (CVs) of both inter-assay and intra-assay repeatability were less than 2.0%,showing good repeatability.The assay was able to specifically detect NiV and HP-PRRSV simultaneously without cross-reaction with classical swine fever virus (CSFV),porcine epidemic diarrhea virus (PEDV),swine influenza virus (SIV),porcine parvovirus (PPV),pseudorabies virus (PRV) and porcine circovirus type 2 (PCV2).Of the 236 samples from pigs for both NiV and HP-PRRSV detection by the established assay,all the samples were negative for NiV,8 samples were HP-PRRSV positive.In conclusion,this assay offers a useful approach for the differential detection of NiV and HP-PRRSV in clinical specimens from the pigs. 相似文献
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为揭示北方农牧交错带地区马铃薯“水改旱”种植对其产量和水分利用的影响,选取中国北方农牧交错带地区27个站点,基于站点的气象数据、土壤数据和管理数据驱动充分验证的APSIM-Potato模型,模拟分析农牧交错带地区灌溉和雨养马铃薯的耗水差异。结果表明:北方农牧交错带地区马铃薯种植连续灌溉10、20 a和30 a的产量分别为15 900~35 600、16 400~34 800 kg·hm-2和16 600~34 800 kg·hm-2,改为旱作后对应的产量分别为12 800~30 600、13 900~29 100 kg·hm-2和12 700~25 500 kg·hm-2;灌溉马铃薯产量均表现为西部较高,旱作马铃薯产量则为东部较高。连续灌溉10、20 a和30 a的播前1 m土层土壤含水量分别为163~388、161~394 mm和154~398 mm,改为旱作后分别下降31.8%、35.3%和36.9%。连续灌溉10、20 a和30 a后的地下水消耗量分别为5 360~21 330、8 910~4... 相似文献
85.
为有效保全苜蓿草颗粒贮藏过程中维生素及营养品质,本研究以紫花苜蓿草颗粒为研究材料,研究贮藏时间(0,60,120,180,240 d,冷冻240 d)与蒙药黄芩(0%,0.5%,1.0%,1.5%)对其维生素含量和营养品质的影响。结果表明:在贮藏过程中,与其他添加量相比,添加1.5%黄芩有利于保存苜蓿草颗粒维生素B5、维生素C和α-维生素E含量。贮藏60 d及以上显著降低了苜蓿草颗粒维生素B1、维生素B3、维生素C和维生素E含量;贮藏120 d时苜蓿草颗粒DM含量趋于稳定;苜蓿草颗粒贮藏180 d后其CP含量显著降低;贮藏0~180 d期间,随着贮藏时间的延长,苜蓿草颗粒NDF和ADF含量显著升高。与常温贮藏240 d相比,冷冻贮藏相同时间有利于保存苜蓿草颗粒中维生素B1、维生素B5、α-维生素E和OM含量,显著降低DM、NDF和ADF含量。因此,1.5%黄芩添加剂有利于保存苜蓿草颗粒维生素含量,且常温贮藏时间不宜超过60天。 相似文献
86.
地下热水的来源研究对地下热水资源量评价和可持续开发利用有重要意义。选取重庆市北温泉作为研究对象,采用水化学分析、D、O同位素以及实时在线监测对其热水来源进行了研究。研究认为:重庆市北温泉泉水为中-低温浅层中性地热水,水化学类型为SO4-Ca型,地下热水的δ 18O值为-8.48‰~-7.09‰,δD值为-55.46‰~-53.26‰,其补给来源为雨水,补给高程为海拔641~1 206 m的岩溶出露区。安装马歇尔槽和CDTP300高分辨率实时在线监测发现:1)地下热水水文地球化学特征在长时间内稳定,体现了稳定的水-岩作用过程;2)北温泉的流量与研究区降雨量呈现出以半年为单位的滞后,雨季偏低,旱季偏高;当场降雨后20 d左右,温泉水的水温和电导率微降,水量增加。地下热水的来源为大气降水,其补给除了长时间长距离的含水层稳定补给外,热水上升过程中受地表水和浅层地下水的补给。 相似文献
87.
Some new concepts of coneD_s convexity,coneD-s quasiconvexity,cone D-s pseudoconvexity,s right derivative of vector value mapping are proposed;and their related properties are discussed;the optimality necessary conditions and sufficient conditions for constrained extremum problem(VP)involving in cone D-s convex mapping are extablished;the relation between local optimal solution and global optimal solution;as well as the relation between weakly efficient solution and efficient solution for (VP) are derived;the corresponding results generalize the related results of classic convex programming. 相似文献
88.
In the analysis of cognition of human brain,the fMRI is often used.Corelation is a common method in fMRI data detecting,but its ability should be improved.Based on the Canonical Correlation Analysis(CCA),the temporal correlation and spatial correlation are used together to detect the activated area in the fMRI data.By the application of the method in simulation data and fMRI data,it shows that this method is effective in the detecting. 相似文献
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Determination to Weighting Coefficient of Combination Forecast Based on Rough Set Theory 总被引:1,自引:0,他引:1
The problem of determination to weighting coefficient is a key and difficulty for combination forecast. A method of determining weighting coefficient based on rough set theory is showed in this paper. Determining weighting coefficient is translated into estimating significance of attributes among rough set. A relation data model about combination forecast is established. Knowledge systems are built through making attribute value into eigenvalue. Under data moving, the weighting coefficients of a combination forecast model are computed by analyzing the dependence and significance of forecasting method for the predicted object. The proposed approach overcomes the subjectivity of traditional determination to weighting coefficient, avoids computing linear or nonlinear extremum problem and makes combination forecast more objective. The validity of the proposed approach is verified with a case. 相似文献