全文获取类型
收费全文 | 7471篇 |
免费 | 432篇 |
国内免费 | 672篇 |
专业分类
林业 | 481篇 |
农学 | 356篇 |
基础科学 | 328篇 |
728篇 | |
综合类 | 3714篇 |
农作物 | 536篇 |
水产渔业 | 364篇 |
畜牧兽医 | 1289篇 |
园艺 | 514篇 |
植物保护 | 265篇 |
出版年
2024年 | 71篇 |
2023年 | 159篇 |
2022年 | 341篇 |
2021年 | 308篇 |
2020年 | 283篇 |
2019年 | 315篇 |
2018年 | 208篇 |
2017年 | 375篇 |
2016年 | 243篇 |
2015年 | 371篇 |
2014年 | 403篇 |
2013年 | 399篇 |
2012年 | 605篇 |
2011年 | 641篇 |
2010年 | 581篇 |
2009年 | 510篇 |
2008年 | 531篇 |
2007年 | 500篇 |
2006年 | 447篇 |
2005年 | 400篇 |
2004年 | 250篇 |
2003年 | 158篇 |
2002年 | 148篇 |
2001年 | 130篇 |
2000年 | 125篇 |
1999年 | 45篇 |
1998年 | 6篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1986年 | 3篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1962年 | 2篇 |
1956年 | 4篇 |
1955年 | 2篇 |
排序方式: 共有8575条查询结果,搜索用时 15 毫秒
71.
TAN Wei-ping XIA Yan WU Bao-jing LI Jing HUANG Hua-rong HUANG Shao-liang MAI Xian-di 《园艺学报》2009,25(12):2399-2402
AIM: To investigate the effect of T-bet plasmid gene transfer to airway on allergen induced airway inflammation in a murine asthmatic model. METHODS: A mouse asthma model was established by sensitization with ovalbumin (OVA). Forty C57BL/6 mice were divided into 4 groups (10 mice in each group): the normal control group (group A), the asthmatic model group (group B), the pcDNA3 plasmid group (group C), and the pcDNA3-T-bet group (group D). The animals in group B, C and D were sensitized and challenged with OVA. The animals in group A were applied with normal saline. pcDNA3 plasmid at dose of 50 μg was intranasally administered at 24 h before intranasal challenges to the mice in group C, and the 50 μg pcDNA3-T-bet plasmid for the mice in group D. Bronchial alveolar lavage fluid (BALF) was collected and lung tissues were resected at 48 h after OVA challenge for later assay. RESULTS: After administration with pcDNA3-T-bet plasmid, high level of T-bet expression at 48 h was detected in the lung tissue by Western blotting. In pcDNA3-T-bet treated asthmatic models, histological evaluation revealed the significant suppression of eosinophil peribronchial and perivascular infiltration, and reduction of epithelial damage. The numbers of eosinophils, neutrophils and lymphocytes in BALF from pcDNA3-T-bet treated mice were significantly reduced compared to those in asthmatic control group (P<0.05). The level of IL-4 in BALF was significantly decreased in pcDNA3-T-bet group compared to that in asthmatic control group (P<0.05), while the level of IFN-γ in BALF was significantly increased in pcDNA3-T-bet group. No significant change of inflammation cells and cytokines in pcDNA3 plasmid group and asthmatic control group was observed (P>0.05). CONCLUSION: Intranasal pcDNA3-T-bet plasmid transfer inhibits asthmatic airway inflammation in the murine asthmatic model, suggesting a new therapeutic strategy for allergic asthma. 相似文献
72.
73.
74.
固定化细胞发酵半纤维素水解液产木糖醇的研究 总被引:4,自引:0,他引:4
固定在海藻酸钙凝胶中的热带假丝酵母(Candida tropicalis)细胞,可有效地利用玉米芯半纤维素水解液生产木糖醇。在摇瓶条件下,采用分批发酵方式,确立了适宜的发酵工艺参数为:32℃,每升氮源含酵母粉2.2g、胰蛋白胨3.7g,发酵液初始pH值6.0,分段改变摇床转速,其中0~24h为210r/min;24~72h为140r/min,固定化细胞凝胶珠与玉米芯半纤维素水解液体积比为1:4。利用固定化细胞重复进行10批次共30d发酵,木糖醇得率平均为73.7%,达到了理论值的80%。固定化细胞密度高、抗逆性强、发酵性能稳定,水解液未经脱色与离子交换便可转化成木糖醇,显示了良好的应用前景。 相似文献
75.
XIA Zi-rong LI Qing XIA Zhen LI Ju-xiang HONG Kui WU Yan-qing WU Qin-hua CHENG Xiao-shu 《园艺学报》2017,33(12):2238-2244
AIM: To investigate the effects of xeroderma pigmentosum group D (XPD) gene on the proliferation of human umbilical arterial smooth muscle cells (HUASMCs) induced by oxidized low-density lipoprotein (Ox-LDL). METHODS: The recombinant plasmid pEGFP-N2/XPD was transfected into HUASMCs by liposome. The cells were divided into blank control group, pEGFP-N2 group, pEGFP-N2/XPD group, Ox-LDL group, Ox-LDL+pEGFP-N2 group and Ox-LDL+pEGFP-N2/XPD group. The proliferation rate of the cells was detected by MTT and EdU assays. The apoptotic rate and cell cycle distribution were analyzed by flow cytometry. The protein levels of XPD, caspase-3, Bcl-2 and Bax were determined by Western blot. RESULTS: Compared with blank control group, the expression of XPD was increased in pEGFP-N2/XPD group (P<0.05). According to the results of MTT and EdU assays, the cell proliferation in pEGFP-N2/XPD group was reduced compared with blank control group (P<0.05). Compared with Ox-LDL group, the cell proliferation in Ox-LDL+pEGFP-N2/XPD group was significantly inhibited (P<0.05). According to the results of flow cytometry, the cell proportion of S phase decreased and the G0/G1-phase cell proportion increased significantly in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group compared with blank control group and Ox-LDL group, repectively (P<0.05). Compared with blank control group and Ox-LDL group, the protein level of Bcl-2 decreased and the protein levels of Bax and cleaved caspase-3 increased in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group, respectively (P<0.05). CONCLUSION: XPD inhibits the proliferation of HUASMCs and promotes their apoptosis, and reduces the promoting effect of Ox-LDL on the proliferation of HUVSMCs. XPD may be the target for treatment of atherosclerosis. 相似文献
76.
AIM:To screen the lentiviral vector carrying siRNA with higher efficiency of suppressing the sphingosine-1-phosphate receptor 2(S1P2) gene expression in the primarily cultured corpus cavernosum smooth muscle cells of spontaneously hypertensive rats (SHR).METHODS:SHR and SD rats (n=5 each) were used for primarily culturing corpus cavernosum smooth muscle cells.The cells were randomly divided into 6 groups:SHR siRNA-1,SHR siRNA-2,SHR siRNA-3,SHR GFP,SHR control (SHR non-transfection group),and SD control (SD rat control group).Each group had 5 samples with 1.0×105 cells of each sample.At 72 h after transfection (MOI=60) with lentiviral vectors carrying S1P2 siRNA into the SHR corpus cavernosum smooth muscle cells,the expression of GFP was observed under fluorescence microscope.The protein expression of S1P2,ROCK1,ROCK2 and eNOS in the corpus cavernosum smooth muscle cells,and the mRNA expression of S1P2,ROCK1 and ROCK2 were determined by by Western blot and RT-PCR.RESULTS:The transfection efficiency of the corpus cavernosum smooth muscle cells in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were>80%.Compared with SHR control group,the mRNA levels and the protein expression of S1P2,ROCK1 and ROCK2 in SHR GFP group showed no remarkable changes,while those in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SD control groups were significantly lower than those in SHR control group (P<0.05).The protein expression of eNOS in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were not significantly changed as compared with SHR control group,but that in SD control group was significantly higher than that in SHR control group.CONCLUSION:Three groups of siRNA lentiviral vectors targeting S1P2 inhibit the expression of S1P2 in the corpus cavernosum smooth muscle cells of SHR,and by silencing the S1P2 expression,the expression of ROCK1 and ROCK2 is inhibited.Among them,siRNA-1 has the highest inhibitory efficiency. 相似文献
77.
本试验旨在通过探究不同桑叶提取段及桑叶粗提取物对小鼠生长性能、抗氧化功能、血清生化指标和炎症细胞因子的影响,筛选出一种较有效的桑叶提取方法。试验使用正己烷、乙酸乙酯、乙醇(85%)和水(60~70℃)制得桑叶提取物,另外使用水煎煮的方法制得桑叶粗提物,分别记为A、B、C、D和E。选取108只5周龄无特定病原体(SPF)级ICR雄性小鼠,随机分为6组,对照组饲喂基础饲粮,试验组在基础饲粮中分别添加桑叶提取物A、B、C、D和E(1 200 mg/kg),每组6个重复,每个重复3只小鼠,预试期3 d,正试期28 d。结果显示:与对照组相比,1)E组小鼠的平均日采食量(ADFI)极显著提高(P<0.01);2) C、D和E组小鼠血清谷胱甘肽过氧化物酶(GSH-Px)活性显著升高(P <0.05),B、D和E组血清总超氧化物歧化酶(T-SOD)活性极显著升高(P<0.01);血清高密度脂蛋白胆固醇(HDL-C)含量显著降低(P<0.05),B和D组血清天门冬氨酸氨基转移酶(AST)活性显著升高(P<0.05),B和E组在血清甘油三酯(TG)含量显著降低(P<0... 相似文献
78.
79.
80.