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71.
概述了配气机构凸轮型线的设计方法。根据配气机构振动特征数ε确定了两种型线函数(组合式和高次方式),对4108型高速柴油机凸轮型线进行了设计计算,并对配气机构进行了动力特性计算。在相同的初始条件下,比较了两种型线的各种性能参数的差别,对两种凸轮型线的优劣作出了评判。 相似文献
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La3+,Ce3+对厌氧颗粒污泥在不同VFA底物中的产甲烷促进效应 总被引:4,自引:0,他引:4
采用静态实验研究了分别添加O.05mg·L^-1La^3+和Ce^3+对不同来源和保存状况条件下不同VFA底物的厌氧颗粒污泥产甲烷的影响。结果表明,乙酸为底物时加入Ce^3+,丙酸加入La^3+或Ce^3+,乳酸加入La^3+时都大大加快了反应初期污泥的产甲烷速率,且对比产甲烷活性SMA也有3%-8%的提高;O.05mg·L^-1La^3+或Ce^3+的加入使污泥利用丙酸的速度加快,对于厌氧反应器的恢复运行有一定的实际意义。甲酸为底物时,La^3+,Ce^3+的加入对污泥产甲烷曲线形状没有影响,对SMA则分别降低了9.21%和3.37%;乙酸添加La^3+在反应初期明显降低了产甲烷速率,但SMA并未降低。厌氧颗粒污泥经长时间低温存放后,原来对其SMA有提高作用的稀土浓度此时可能会作用不明显甚至会降低SMA;低浓度稀土离子对污泥产甲烷活性产生抑制作用时,相同质量浓度下Ce^3+比La^3+对污泥的抑制作用小;驯化培养过程有利于污泥对稀土的适应。 相似文献
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本文综述了生活污水厌氧消化系统相关后处理技术的技术特点和研究进展,着重分析了好氧处理、稳定塘处理及土地处理三种后处理技术方法,简要介绍了户用一体化装置的研究及应用状况。 相似文献
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SU Ying JIN Chun SUN Su-min LI Zhang-hao XIA Si-wei ZHANG Zi-li ZHANG Feng SHAO Jiang-juan ZHENG Shi-zhong 《园艺学报》2021,36(12):2283-2288
RNA-binding proteins (RBP) are molecules with a variety of biological functions discovered in recent years. Among them, HuR is an important RBP, widely expressed in various tissues of the body, and is a member of the Hu/embryonic lethal abnormal vision (ELAV) protein family. It mainly affects the expression levels of target genes in the cells by regulating the stability and/or translation efficiency of the mRNA of the genes, thus participating in the regulation of cell life activities. In recent years, more and more studies on HuR have revealed its important role in inflammation and cancer. This review summarizes the functions of HuR and probes into the regulation of HuR functions. In addition, the roles of HuR in the occurrence and development of hepatocellular carcinoma (HCC) are also discussed, which provides important information for exploring the pathogenesis, biomarkers and therapeutic targets of HCC. 相似文献
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AIM To construct the mouse embryonic stem cell (ESC) line with stable pancreatic and duodenal homeobox 1 (Pdx1 ) expression by Tet-On system, which may lay a foundation for further research on the differentiation of Pdx1+ definitive endoderm cells into pancreatic cells. METHODS The Pdx1 -overexpressing lentiviral vector with green fluorescent protein marker and puromycin resistance was constructed by Tet-On system and was used to infect the mouse ESC. The cells were divided into 3 groups: blank control group (ESC group), empty lentivirus control group (PDX1- ESC group) and Pdx1 lentivirus transfection group (PDX1+ ESC group). Flow cytometry was used to detect the transfected cells after screening by doxycycline (DOX). The function of Tet-On system and the expression of Pdx1 gene were detected. The transfected cells in PDX1- ESC group and PDX1+ ESC group were sorted by flow cytometry, and constructed ESC line with stable expression of Pdx1 and negative control ESC line were verified. RESULTS (1) The positive rates of transfected cells in PDX1- ESC group and PDX1+ ESC group were 90.72% and 94.01% after screening by DOX, respectively. The positive rates of transfected cells in PDX1- ESC group and PDX1+ ESC group was 97.84% and 98.13% after sorting by flow cytometry, respectively. (2) With DOX, green fluorescence was observed in PDX1- ESC group and PDX1+ ESC group. The mRNA and protein expression of Pdx1 was significantly increased in PDX1+ ESC group (P <0.05). Without DOX, no green fluorescence was observed in the cells of the 3 groups, and no significant difference in the mRNA and protein expression of Pdx1 was observed (P >0.05). (3) After 3 months of cryopreservation, the cell lines still survived in resuscitation culture and were regulated by DOX. CONCLUSION Using Tet-On system, the mouse ESC line with inducible Pdx1 expression were successfully established and could be used as an effective cell model to research the differentiation of Pdx1+ definitive endoderm cells into pancreatic cells. 相似文献
80.
以Micro-Tom番茄为试材,在营养液水培条件下,对果实发育成熟期补光(B)、果实发育期补光(B1)、果实成熟期补光(B2)下番茄植株生长、叶片光合参数、果实质量、果实品质、根系形态、矿物质离子平均吸收速率及其相互关系进行了研究,以期明确增补蓝光对番茄生长、果实品质、养分吸收的调控效果.结果 表明:B1处理能显著促进番茄的生长与果实膨大,增加果数、提高果实质量,与B处理的效果一致;B处理显著提高了番茄根系生长各项指标,B1处理显著促进主根的伸长,而B2处理对番茄根系生长无显著影响;B1处理或者B2处理显著提高了番茄果实中的有机酸含量,可溶性糖、维生素C含量、糖酸比与不补光处理差异不显著;B1与B补光处理显著提高了NO3-N、PO4-P、K+、Ca2+、Mg2+平均吸收速率;B1与B补光处理通过促进PO4-P与K+吸收,间接促进坐果,加快番茄果实发育,促进成熟与果实着色.因此,B1补光处理能达到与B补光处理相同的效果,优于B2处理.在低耗能补光策略上可以考虑采用果实发育期补光. 相似文献