N、P、K三要素对水稻的蛋白质效应分析

利用数学模型研究了肥料N、P、K对糙米蛋白质含量的影响，分析了单因素效应，双因素效应、边际效应。得出三因素对糙米蛋白质含量的贡献率大小依次为N1．9744、P2O5 1．339、K2O1．2285；N和P互作明显，高N、高P有利于蛋白质含量的提高。     

乳酸菌Lact.1和Lact.2降解胆固醇的研究

从发酵菠萝、桔子中分离选育到2菌株,经鉴定为乳酸菌,将菌株接种到含有胆固醇MRS-CHOL液体培养基中,在38℃条件下,恒温培养3d后,测定胆固醇的降解率、pH值和菌数的变化．结果表明：2菌株都具有降解胆固醇能力,随着培养时间的延长和接种量的增加,降解率都呈现增加趋势;在基质中胆固醇浓度较低时,胆固醇降解率都逐渐增高,而当浓度不断增加时,胆固醇降解率都出现下降趋势．但2菌株之间又存在不同变化幅度．在降解过程中,pH值和菌数也相应发牛了变化。     

固定化细胞发酵半纤维素水解液产木糖醇的研究

固定在海藻酸钙凝胶中的热带假丝酵母(Candida tropicalis)细胞，可有效地利用玉米芯半纤维素水解液生产木糖醇。在摇瓶条件下，采用分批发酵方式，确立了适宜的发酵工艺参数为：32℃，每升氮源含酵母粉2．2g、胰蛋白胨3．7g，发酵液初始pH值6．0，分段改变摇床转速，其中0～24h为210r／min；24～72h为140r／min，固定化细胞凝胶珠与玉米芯半纤维素水解液体积比为1：4。利用固定化细胞重复进行10批次共30d发酵，木糖醇得率平均为73．7％，达到了理论值的80％。固定化细胞密度高、抗逆性强、发酵性能稳定，水解液未经脱色与离子交换便可转化成木糖醇，显示了良好的应用前景。     

安徽省大别山油茶选优研究

安徽省大别山地区气候多变,油茶品种复杂,油茶产量高低不一,从而导致油茶的经济效益低下.为了提高该地区油茶产量,从油茶实生林分收集和筛选具有高产、抗病虫、抗寒、早花避寒等优良性状的油茶优异新种质,为安徽省大别山地区提供适栽的具有早花、抗寒能力的油茶苗木,推动北缘地区油茶产业的发展.     

不同基本苗和栽插规格对杂交水稻4001 S/黄莉占制种机插父本群体生长发育的影响

为探究机械化制种父本最适宜的基本苗数和栽插规格,选用4001S/黄莉占制种组合,设计父本2株、4株和6株3种不同基本苗数,20cm×30cm、24cm×30cm、32cm×30cm、20cm×(20~40)cm和25cm×(20~40)cm五种不同栽插规格,2期播种同时栽插,研究了供试父本群体的苗情动态、抽穗开花动态和穗粒结构。结果表明:采用机插秧6株苗能够显著增加父本的分蘖数和有效穗数,并能增加单位面积颖花数;采用20cm×(20~40)cm和25cm×(20~40)cm两种栽插规格能够显著提高最高苗、有效穗和单位面积颖花数,且25cm×(20~40)cm规格的盛穗历期最长,达到5d;2期父本播期相差6d,抽穗历期可延长1d。说明父本黄莉占以单穴6苗并按20cm×(20~40)cm或25cm×(20~40)cm规格机插可以获得较高的单位面积颖花数。     

Effect of xeroderma pigmentosum group D gene on proliferation of human umbilical arterial smooth muscle cells induced by Ox-LDL

AIM: To investigate the effects of xeroderma pigmentosum group D (XPD) gene on the proliferation of human umbilical arterial smooth muscle cells (HUASMCs) induced by oxidized low-density lipoprotein (Ox-LDL). METHODS: The recombinant plasmid pEGFP-N2/XPD was transfected into HUASMCs by liposome. The cells were divided into blank control group, pEGFP-N2 group, pEGFP-N2/XPD group, Ox-LDL group, Ox-LDL+pEGFP-N2 group and Ox-LDL+pEGFP-N2/XPD group. The proliferation rate of the cells was detected by MTT and EdU assays. The apoptotic rate and cell cycle distribution were analyzed by flow cytometry. The protein levels of XPD, caspase-3, Bcl-2 and Bax were determined by Western blot. RESULTS: Compared with blank control group, the expression of XPD was increased in pEGFP-N2/XPD group (P<0.05). According to the results of MTT and EdU assays, the cell proliferation in pEGFP-N2/XPD group was reduced compared with blank control group (P<0.05). Compared with Ox-LDL group, the cell proliferation in Ox-LDL+pEGFP-N2/XPD group was significantly inhibited (P<0.05). According to the results of flow cytometry, the cell proportion of S phase decreased and the G₀/G₁-phase cell proportion increased significantly in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group compared with blank control group and Ox-LDL group, repectively (P<0.05). Compared with blank control group and Ox-LDL group, the protein level of Bcl-2 decreased and the protein levels of Bax and cleaved caspase-3 increased in pEGFP-N2/XPD group and Ox-LDL+pEGFP-N2/XPD group, respectively (P<0.05). CONCLUSION: XPD inhibits the proliferation of HUASMCs and promotes their apoptosis, and reduces the promoting effect of Ox-LDL on the proliferation of HUVSMCs. XPD may be the target for treatment of atherosclerosis.     

Screening of lentiviral vectors carrying effective siRNA targeting S1P2 gene

AIM:To screen the lentiviral vector carrying siRNA with higher efficiency of suppressing the sphingosine-1-phosphate receptor 2(S1P2) gene expression in the primarily cultured corpus cavernosum smooth muscle cells of spontaneously hypertensive rats (SHR).METHODS:SHR and SD rats (n=5 each) were used for primarily culturing corpus cavernosum smooth muscle cells.The cells were randomly divided into 6 groups:SHR siRNA-1,SHR siRNA-2,SHR siRNA-3,SHR GFP,SHR control (SHR non-transfection group),and SD control (SD rat control group).Each group had 5 samples with 1.0×10⁵ cells of each sample.At 72 h after transfection (MOI=60) with lentiviral vectors carrying S1P2 siRNA into the SHR corpus cavernosum smooth muscle cells,the expression of GFP was observed under fluorescence microscope.The protein expression of S1P2,ROCK1,ROCK2 and eNOS in the corpus cavernosum smooth muscle cells,and the mRNA expression of S1P2,ROCK1 and ROCK2 were determined by by Western blot and RT-PCR.RESULTS:The transfection efficiency of the corpus cavernosum smooth muscle cells in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were>80%.Compared with SHR control group,the mRNA levels and the protein expression of S1P2,ROCK1 and ROCK2 in SHR GFP group showed no remarkable changes,while those in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SD control groups were significantly lower than those in SHR control group (P<0.05).The protein expression of eNOS in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were not significantly changed as compared with SHR control group,but that in SD control group was significantly higher than that in SHR control group.CONCLUSION:Three groups of siRNA lentiviral vectors targeting S1P2 inhibit the expression of S1P2 in the corpus cavernosum smooth muscle cells of SHR,and by silencing the S1P2 expression,the expression of ROCK1 and ROCK2 is inhibited.Among them,siRNA-1 has the highest inhibitory efficiency.     

基于不同空间插值的污染场地土壤锑修复范围预测研究

以污染场地土壤锑超标地层为研究对象,结合土壤每层锑含量的分布特点,分析和比较了克里格插值模型(OK、UK)、反距离插值(IDW)、泰森多边形(TIN)和分区预测模型(OK+TIN)对不同深度土壤锑修复范围及预测精度的差异。结果表明：各方法确定的0～1 m地层修复面积大小顺序为IDW>OK=UK> TIN>OK+TIN,修复面积依次为74 154、57 427、37 338、32 707 m²;各方法确定的1～3.8 m地层修复面积大小顺序为IDW>UK>TIN>OK+TIN>OK,修复面积依次为13 089、11 030、9 660、2 183、1 231 m²,克里格插值带来的平滑作用导致超标范围的遗漏和未超标区域纳入修复范围。在受成本和污染物空间分布制约的情况下,TIN方法从几何原理的角度可以较为准确地反映不同深度土壤锑的修复范围;克里格插值(包括OK+TIN方法)和反距离插值受数据分布假设的约束,平滑效应明显,估计精度受限。     

优质高产牧草皇竹草综述

杂交巨象草又称皇竹草,是由象草和非洲狼尾草杂交育成的一种优质高产牧草品种,具有适应性广,抗逆性强,产量高,粗蛋白和糖分含量较高,叶量较多的一种热带饲用植物,适宜热带亚热带及我国南方温带地区栽培种植。