新辟红壤性稻田不同施肥模式的效应

在江永县圣人山农业综合开发区，以由耕型砂岩红壤开辟的新稻田为供试土壤，以杂交水稻华联２号（早稻）和威优．６４（晚稻）为供试作物，探讨了新辟红壤性稻田的地力贡献及不同施肥模式对早、晚稻产量及其生育和营养特性以及土壤肥力的影响，并由此提出了新辟红壤性稻田上早、晚稻的适宜施肥模式．     

棉花胞质不育育性恢复候选基因载体构建及其功能初步分析

棉花细胞质雄性不育是研究杂种优势利用和质核互作机理的理想材料。本实验对棉花细胞质雄性不育育性恢复基因ZH46-3437利用pBI121载体构建了过表达载体。同时构建了amiRNA干扰载体即amiR3437,利用病毒诱导基因沉默体系pCLCrV浸染F1植株的子叶。转基因植株的相对荧光定量数据表明,amiR3437的相对表达量上调,而ZH46-3437的相对表达量下调,利用碘-碘化钾染色法鉴定转基因出现半不育性状,表明ZH46-3437基因可能与棉花花粉育性有关。     

覆盖光降解地膜对土壤污染及棉花生育影响的研究

自１９８４年以来，对国内外３０种不同型号的可控制降解时间的光降解地膜，进行了棉花田间效应研究认为，光解膜在自然曝晒下在一定时间范围内能够自行降解，与普通地膜相比具有相同的土壤环境效应和相近的增产效果。降解产物对土壤的矿质元素含量无明显影响，也未发现有害物质产生。在棉田应用时，诱导期控制在６０天左右，此后，降解过程越短越好，最长不宜超过１２０天。     

珍稀油料树种琴叶风吹楠种子萌发习性

从西双版纳地区采集野生琴叶风吹楠(Horsfieldia pandurifolia)的种子开展萌发习性研究。结果表明:成熟的新鲜种子沙床催芽萌发率在96%以上,幼苗移栽成活率高达100%;相同植株的种子土壤播种萌发率为80.00%～86.27%,而整个果实播种萌发率仅有9.09%～33.33%;模拟野生环境下浅覆土的种子萌发率为55%～95%,不覆土的种子萌发率为20%～65%;8个月苗龄土壤播种苗11个家系平均株高为24.70～47.43cm,变异系数为21.95%～41.26%,平均地径为7.16～11.08mm,变异系数为16.62%～35.12%,家系间变异大。     

多抗、优质、丰产、中熟早籼水稻新品种的选育

经数年抗性鉴定表明,杂交育成的中熟早籼新品种HA79317-4和湘早籼3号(HA79317-7)抗白叶枯病、稻瘟病、褐飞虱、白背飞虱、黑尾叶蝉,而且抗性稳定。1981—1987年品比、省区试、南方稻区区试及大面积生产实践结果表明,亩产400公斤左右,比当地同熟期主栽品种增产5.81—11.01％,于1985年评为全国优质米品种,获农牧渔业部奖状和金杯奖。这两个新品种适宜于长江中、下游流域和以南地区种植,至1987年省内外已推广面积达1000万亩以上。     

Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method

Background  

Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used for studies of subcellular protein localization, promoter activity, and in vivo protein-protein interactions. This method requires cutting leaves into very thin slivers to collect mesophyll cell protoplasts, a procedure that often causes cell damage, may yield only a few good protoplasts, and is time consuming. In addition, this protoplast isolation method normally requires a large number of leaves derived from plants grown specifically under low-light conditions, which may be a concern when material availability is limited such as with mutant plants, or in large scale experiments.     

姑婆山国家森林公园景观格局变化分析

根据姑婆山国家森林公园1999年和2009年森林调查资料,在ArcGIS和Fragstas 3.3的支持下对姑婆山国家森林公园景观格局动态变化进行研究。结果表明：研究期间,研究区主要景观类型没有变化,阔叶树、灌木林、杉木林3种主要景观类型面积占总面积的比例由80.44%提高到94.51%;景观基质均为阔叶林,占总面积比例由63.63%提高到75.82%;总斑块数量由301块下降到174块;平均形状指数呈下降趋势;多样指数由1.233下降到0.862;优势度指数则由0.846升高至1.217。总体表现为针叶林向针阔混交林,进而向阔叶林演替,景观趋向连续和稳定。     

Dietary phosphorus requirement of GIFT strain of Nile tilapia Oreochromis niloticus reared in freshwater

A study was conducted to estimate the optimum requirement of dietary available phosphorus for GIFT strain of Nile tilapia Oreochromis niloticus. Six purified diets were formulated to contain graded levels (0 (control diet), 2.9, 4.8, 7.6, 9.1 and 10.9 g kg^?1 diet) of available phosphorus. Each diet was fed to triplicate groups of 12 fish with initial average weight (46.03 ± 2.14) g for 8 weeks. The results showed that fish fed the three lowest phosphorus diets (0, 2.9 and 4.8 g kg^?1) had significantly lower weight gain rate, specific growth rate (SGR) and feed efficiency than those fed the other diets (P < 0.05). The survival rate of fish fed the control diet was significantly lower than that of the fish fed the other diets (P < 0.05). Whole body viscerosomatic index and crude lipid content decreased significantly with increasing dietary available phosphorus levels (P < 0.05), while the contents of crude ash, calcium, phosphorus in the whole body and vertebrae showed the opposite trend (P < 0.05). The blood chemistry analysis showed that dietary available phosphorus had significant effects on serum phosphorus concentration, enzyme activities of alkaline phosphatase and parathyroid hormone level. Quadratic curve analysis based on SGR indicated that the minimum dietary requirement of available phosphorus for maintaining optimal growth of tilapia was 8.6 g kg^?1.     

中职学校德育课课程改革初探

随着新一轮的中职学校德育课课程改革的深入开展和中职学生主体意识的增强,传统的德育课教学有许多方面需要进一步改进和完善.在中职学校德育课教学中实施高效良性的师生互动,能提高德育课的教学实效和学生的思想道德素质,为学生职业生涯发展奠定基础.