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Yun Sang CHO Sang Eun LEE Jong-Tae WOO Jinsik OH Hwan Won CHOI Jin Hyeok KWON Jeong-Tae KIM Gunwoo HA Sukchan JUNG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1631
Bovine tuberculosis (bTB) is a zoonosis caused by Mycobacterium bovis. Test-and-cull protocols and gross pathological examinations of abattoir animals as well as milk pasteurization have been implemented to prevent the spread of tuberculosis from animals to humans worldwide. Despite the importance of precise and rapid diagnostic tests, conventional methods including intradermal skin tests and γ-interferon assays are limited by the high rate of false-negative results for cattle in the late infectious stage and due to laborious and time-consuming procedures. Therefore, antibody detection methods such as enzyme-linked immunosorbent assay (ELISA) are urgently needed to supplement the established approaches and expand the diagnostic window. This study was conducted to develop a bTB ELISA by evaluating recombinant and native proteins and various assay parameters. We produced recombinant MPB70 and SahH (M70S) and a native 20-kDa protein (20K) and optimized the ELISA protocol. The 20K ELISA showed 94.4% sensitivity and 98.2% specificity with an optimal sample-to-positive ratio cut-off of 0.531. The sensitivity and specificity of M70S ELISA were 94.4% and 97.3%, respectively, with an optimal sample-to-negative ratio cut-off of 1.696. Both assays showed acceptable diagnostic efficiency and could be used for bTB diagnosis in combination with established methods for herd screening and to expand the diagnostic window. 相似文献
33.
Jeongyun CHOI Aritra ROY CHOUDHURY Myung-Min OH Denver I. WALITANG Tongmin SA 《土壤圈》2022,32(2):364-368
Dear Editor,Industrial emissions have played a major role in damaging the ozone layer,leading to increased atmospheric penetration of ultraviolet B (UV-B)(290–320 nm) radiation (Jordan,2002).The recovery rate of the damaged ozone layer in the mid-latitudes and tropics has been insignificant since 1979(Chipperfield et al.,2017),even after the adoption of the Montreal Protocol (Protocol,1987),and the recovery process is expected to take many decades.In the meantime,UV-B radiation continues to penetrate the atmosphere (McKenzie et al.,2011;Bais et al.,2019).The characteristic short length and high energy of this radiation wavelength can directly affect major molecules,such as DNA,proteins,and lipids,which are important to living beings (Hideg et al.,2013).Rice is one of the major global crops,which is generally cultivated at lower latitudes and can potentially be exposed to a higher flux of UV radiation due to greater solar angles and longer duration of sunlight (Kataria and Guruprasad,2012). 相似文献
34.
Se-Hoon KIM Seo-In CHOI Kun-Ho SONG Kyoung-Won SEO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):465
A 9-year-old female mixed-breed dog presented with ascending flaccid tetraparesis, and a 5-year-old castrated male Poodle dog presented with ventroflexion of neck, dysphonia, and hindlimb weakness, which progressed to acute ascending tetraparesis. Both dogs were fed raw poultry for over 9 and 5 years, respectively. Blood examination and other test results were normal or unrelated to the present case. Fecal polymerase chain reaction analysis in the Poodle dog was positive for Clostridium perfringens and Campylobacter jejuni. Tetraparesis improved with supportive care in both dogs. Human IV immunoglobulin was only administered to the Poodle dog, which showed a shorter recovery (12 days compared to 34 days in the mixed-breed dog). Both dogs returned to normal conditions eventually. 相似文献
35.
MIN‐SU LEE AH‐RA LEE MI‐AE JUNG IN‐HYE LEE JI‐HYE CHOI HYUN‐WOO CHUNG SOON‐WUK JEONG SANG‐SOEP NAHM KI‐DONG EOM 《Veterinary radiology & ultrasound》2010,51(6):670-673
We evaluated the whole body distribution of 2‐deoxy‐2‐[18F]fluoro‐d ‐glucose (18F‐FDG) in seven beagle dogs using positron emission tomography/computed tomography. The mean and maximum standard uptake values (SUV) for various tissues were computed. The SUV of the aortic blood pool was 0.65±0.19. Moderate uptake was present in brain (3.40±1.01). Mild uptake was present in orbital muscles, soft palate, laryngeal and pharyngeal region, mandibular salivary gland, myocardium, liver, pancreas, kidney, and intestine. 18F‐FDG uptake would be normally higher in these tissues because of normal physiologic activity. Mean and maximum SUV values of the eye, skeletal muscle, bone tissue, spleen, adrenal gland, stomach, tongue, gall bladder, and lung were similar to or lower than that of the aortic blood pool. These data provide a normal baseline for comparing pathologic 18F‐FDG uptake. 相似文献
36.
Chikara MASUTA Yuko SESHIMO Motomi MUKOHARA Hye Jin JUNG Shigenori UEDA Ki Hyun RYU Jang Kyung CHOI 《Journal of General Plant Pathology》2002,68(2):163-168
We analyzed the evolutionary histories of two lily strains of Cucumber mosaic virus (CMV) isolated in Japan and Korea (HL- and Ly2-CMVs). They share common biological characteristics in that their host ranges
are very restricted perhaps from a unique adaptation to lily plants. Although HL and Ly2 were isolated independently from
different lily species in separate countries, their RNA3 sequences had a very high sequence similarity (97%). The evolutionary
relationships between the two isolates were characterized by comparing their phylogenetic trees for the 3a and CP genes. The
two lily CMVs always formed a distinct cluster within subgroup IB in 3a, but within IA in CP. Together, the phylogenetic tree
topology and the sequence identity between the two lily CMVs suggest that they evolved from a common progenitor.
Received 5 November 2001/ Accepted in revised form 11 January 2002 相似文献
37.
Adiposity, fatty acid composition, and delta-9 desaturase activity during growth in beef cattle 总被引:3,自引:1,他引:3
Stephen B. SMITH David K. LUNT Ki Y. CHUNG Chang B. CHOI Ron K. TUME Meiji ZEMBAYASHI 《Animal Science Journal》2006,77(5):478-486
Oleic acid (18:1n‐9) is the most abundant fatty acid in bovine adipose tissue. Because most of the lipid in bovine muscle is contributed by intramuscular adipocytes, oleic acid also is the predominant fatty acid in beef. In many species, the concentration of oleic acid in adipose tissue is dictated by the average concentration of oleic acid in the diet, but in ruminant species such as beef cattle, oleic acid is hydrogenated largely to stearic acid by ruminal microorganisms. In these species, the concentration of oleic acid in adipose tissue is dependent upon the activity of Δ9 desaturase, encoded by the stearoyl coenzyme A desaturase (SCD) gene. Expression of the SCD gene is essential for bovine preadipocyte differentiation, and desaturase gene expression and catalytic activity increase dramatically as adipose tissue mass increases after weaning. Feeding a hay‐based diet to American Wagyu steers to a typical Japanese bodyweight endpoint (650 kg) markedly stimulated desaturase enzyme activity as well as the accumulation of both oleic acid and intramuscular lipid, but the increase in oleic acid and intramuscular lipid was much less in hay‐fed Angus steers. Increasing the concentration of oleic acid improves the palatability and healthiness of beef, and Korean Hanwoo and Japanese Black (and American Wagyu) seem especially well adapted to accumulate oleic acid in their adipose tissue. 相似文献
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Mihyun CHOI Namsoon LEE Seoyeon KEH Heeyeon CHOI Yoonji YIM Hyunwook KIM Joohyun JUNG Mincheol CHOI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):229-231
A 3-year-old, intact female Pomeranian presented with a 1-month history of coughing. Thoracic radiography showed focal infiltration of the left cranial lung lobe and widening of the cranial mediastinum. Subsequent computed tomography revealed torsion of the caudal segment of the left cranial lung lobe, which was confirmed by exploratory thoracotomy. There was no apparent underlying etiology for the condition. To the authors’ knowledge, this is the first report of lung lobe torsion in this breed and the first detailed CT imaging report for segmental lung lobe torsion. 相似文献
40.
Jin Young CHUNG Dae Young YOO Wooseok IM Jung Hoon CHOI Sun Shin YI Hwa Young YOUN In Koo HWANG Je Kyung SEONG Yeo Sung YOON 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):167-173
In the current study, we investigated whether electroacupuncture (EA) can inhibit pathological reductions in neurogenesis. Zucker diabetic fatty (ZDF) rats at 7 weeks of age were anesthetized with zoletil, and sham-acupuncture or EA at the Zusanli (ST36) and Baihui (GV20) acupoints was administered once a day for 5 weeks. In the ZDF group that received sham-EA (ZDF-Sham group), the blood glucose level was significantly increased together with age as compared to the control littermates [Zucker lean control (ZLC) rat]. In contrast, proliferating cells and differentiated neuroblasts were significantly decreased in the ZDF-Sham group compared to the ZLC group. Although EA treatment decreased blood glucose levels, this was not statistically significant when compared to blood glucose levels changes in the ZDF-Sham group. However, proliferating cells and differentiated neuroblasts were significantly increased with EA in ZDF rats as compared to those in the ZDF-Sham group.
Brain-derived neurotrophic factor (BDNF) levels were significantly decreased in hippocampal homogenates of ZDF-Sham group compared to those in the ZLC group. The EA treatment significantly increased the BDNF levels compared to those in the ZDF-Sham group, and BDNF levels in this group were similar to those in the ZLC group. These results suggest that EA at ST36 and GV20 can ameliorate the reductions in proliferating cells and differentiated neuroblasts in the dentate gyrus induced by type-2 diabetes without significantly reducing blood glucose levels with increasing BDNF levels. 相似文献