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91.
P. Boulanger G. L. Bannister A. S. Greig D. P. Gray G. M. Ruckerbauer N. G. Willis 《Canadian journal of veterinary research》1967,31(1):16-23
African swine fever immunofluorescent conjugates were prepared in swine and used successfully in the demonstration of viral antigen in frozen tissue sections and in inoculated tissue culture cells. Cross reactivity was observed with the six strains used in the inoculation of swine. The high antibody content of the serum of immune swine did not interfere with demonstration of the antigen in frozen tissue sections of certain of their organs. The localisation and extent of antigen varied with the stage of infection. The virus was demonstrated in spleen and other organs as early as after one day of pyrexia and until after death of the animal. A pool of hog cholera and African swine fever conjugates stained with dyes of different colours was used in the localisation of respective antigens in experimental mixed infection. 相似文献
92.
Willis AM Anderson DE Gemensky AJ Wilkie DA Silveira F 《American journal of veterinary research》2000,61(12):1542-1544
OBJECTIVE: To estimate intraocular pressure (IOP) in eyes of healthy camelids, using applanation tonometry. ANIMALS: The eyes of 34 camelids (16 llamas [Lama glamal and 18 alpacas [L. pacos]) that did not have major abnormalities of the ocular surface or intraocular abnormalities. PROCEDURE: Tonometry measurements were obtained from each eye 3 times during a 24-hour period. Each measurement was the mean of several corneal applanations obtained by use of an applanation tonometer. Data were analyzed, using an ANOVA for a repeated-measures design. RESULTS: Mean (+/- SEM) IOP of llamas and alpacas was 13.10+/-0.35 and 14.85+/-0.45 mm Hg, respectively. Range of IOP was 7 to 18 mm Hg for llamas and 11 to 21 mm Hg for alpacas. Mean IOP of llamas was significantly less than the mean IOP of alpacas. Significant differences in IOP were not detected between the right and left eye of animals. Significant differences in IOP were not attributed to sex, age, or time of measurement within llamas or alpacas. CONCLUSIONS AND CLINICAL RELEVANCE: Establishing the mean and range of IOP of clinically normal llamas and alpacas provides a frame of reference that is important for use in a complete ophthalmic examination of camelids, which can assist clinicians in the diagnosis of glaucoma and uveitis. Reasons for the difference in mean IOP between llamas and alpacas are unknown. Although the difference may be unimportant clinically, this finding reiterates the fact that caution must be used when extrapolating IOP among species. 相似文献
93.
Ridyard AE Corcoran BM Tasker S Willis R Welsh EM Demetriou JL Griffiths LG 《The Journal of small animal practice》2000,41(12):558-561
Four cases of spontaneous laryngeal paralysis in juvenile white-coated German shepherd dogs are described. The presenting signs were typical for laryngeal paralysis, with stridor present in all four cases. Laryngoscopy revealed bilateral laryngeal paralysis in three cases, and unilateral paralysis in one. Concurrent megaoesophagus was also identified in one dog. All dogs underwent surgical treatment for laryngeal paralysis. Euthanasia was performed in one case due to intractable regurgitation and aspiration pneumonia. A possible association with white coat colour is discussed. 相似文献
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95.
C Marschner C Flanagan DP Higgins MB Krockenberger 《Australian veterinary journal》2014,92(5):177-178
A retrospective review of case records of ultrasonography and necropsy outcomes of 62 koalas was used to investigate the accuracy of ultrasonography in assessing koala urogenital tract structural disease at the Port Macquarie Koala Hospital. The results showed high concordance, supporting ultrasonography as an effective tool for evaluating structural disease of the koala urogenital tract, most commonly seen with chlamydiosis. The study also illustrates the advances benefiting animal welfare that can be made by wildlife carer groups through using a scientific, evidence‐based approach. 相似文献
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98.
A linear photodiode array has been used as the photodetector element in a new ultraviolet-visible detection system for high-performance liquid chromatography. This array allows simultaneous acquisition of light intensity data at all wavelengths between 190 and 600 nanometers. By use of a computer network concept in the electronics, this detection system can process eight different chromatographic signals simultaneously in real time and acquire spectra manually or automatically. Detector response times are variable and can be as low as 0.040 second, and bandwidth selection is variable from 4 to 400 nanometers. These characteristics permit fast chromatographic techniques and user-selectable signal-to-noise ratio enhancement. Spectra can be acquired in 10 milliseconds, permitting qualitative characterization at several different points on a single peak without destroying chromatographic signal integrity. Examples illustrate applications in fast high-performance liquid chromatography peak purity determination, and postanalysis data reduction. 相似文献
99.
Expansion of flood irrigation in the Lower Macquarie Valley of New South Wales, Australia, has been suggested as a major
cause of increased groundwater recharge. The aim of this study was to estimate deep percolation under irrigation on two soils
in the valley, in order to infer groundwater recharge. Three methods were used; water balance, Darcian flux calculations and
chloride mass balance modelling. Chloride mass balance modelling and the water balance method gave comparable estimates of
deep percolation for each soil. Chloride mass balance modelling was identified as the most reliable method for estimating
deep percolation, but only gave an estimate for the entire growing season. These estimates were 214 and 104 mm for a cracking
clay and red brown earth, respectively. While there is potentially greater error associated with estimates obtained using
the water balance, this technique provided estimates of deep percolation for each individual irrigation. Results of the water
balance indicated that deep percolation was greatest early in the growing season, following initial wetting of the soil, when
the crop had a low leaf area index. Results calculated using Darcian flux equations were highly variable, and were therefore
unreliable estimates of deep percolation. Groundwater recharge, inferred from estimates of deep percolation determined with
the chloride mass balance model, was used to estimate the magnitude of potential annual groundwater rise. The potential groundwater
rise during the 1992/1993 cotton growing season ranged from 465 mm beneath the cracking clay to 267 mm under the red brown
earth. It is suggested that groundwater recharge and rise were highly dependent on the weather conditions prevailing during
this period.
Received: 24 January 1997 相似文献
100.