Multiple Ebola virus transmission events and rapid decline of central African wildlife

Several human and animal Ebola outbreaks have occurred over the past 4 years in Gabon and the Republic of Congo. The human outbreaks consisted of multiple simultaneous epidemics caused by different viral strains, and each epidemic resulted from the handling of a distinct gorilla, chimpanzee, or duiker carcass. These animal populations declined markedly during human Ebola outbreaks, apparently as a result of Ebola infection. Recovered carcasses were infected by a variety of Ebola strains, suggesting that Ebola outbreaks in great apes result from multiple virus introductions from the natural host. Surveillance of animal mortality may help to predict and prevent human Ebola outbreaks.     

Flow and storage in groundwater systems

The dynamic nature of groundwater is not readily apparent, except where discharge is focused at springs or where recharge enters sinkholes. Yet groundwater flow and storage are continually changing in response to human and climatic stresses. Wise development of groundwater resources requires a more complete understanding of these changes in flow and storage and of their effects on the terrestrial environment and on numerous surface-water features and their biota.     

Half-sib progeny evaluation and selection of potatoes resistant to the US8 genotype of Phytophthora infestans from crosses between resistant and susceptible parents

The objectives of this study were to evaluate the use of potato (Solanum tuberosum L.) late blight (Phytophthora infestans (Mont.) de Bary) resistant parents in cultivar development and identify superior clones possessing moderate to high late blight resistance combined with acceptable maturity and tuber quality. Ninety-five crosses were made between eight unadapted parents with reported late blight resistance (B0718-3, Bertita, Bzura, Greta, Libertas, Stobrawa, Tollocan and Zarevo) and susceptible parents (cultivars or advanced breeding clones) adapted to North American growing conditions. A total of 408 field selected clones were assessed for late blight resistance in the greenhouse and in the field using a mixture of US8 P. infestans isolates (A2 mating type, metalaxyl resistant) that overcame all known R-genes except R8 and R9. Clones with ≤ 10% infected foliar area in the greenhouse test or ≤ 0.30 RAUDPC (relative area under the disease progress curve) value in the field in 1998 were re-tested in 1999. A total of 118 (29% of 408) putative late blight resistant clones were selected. The eight late blight resistant parents differed in both the ability to transmit late blight resistance and in the level of resistance transmitted to the progeny. The Tollocan and B0718-3 families (half-sib progeny) had the greatest degree of resistance and frequency of resistant clones. Scott-Knott cluster analysis ranked 79 clones (67% of 118) in the high and moderate late blight resistant groups. Among these 79 clones, 19 clones had vine maturity equal to or earlier than mid-season combined with acceptable tuber quality. Further selection in 2000 resulted in eight advanced selected clones (six from Tollocan and two from B0718-3 families) with the same level of resistance as the parent combined with vine maturity and tuber quality equivalent to Atlantic, a standard cultivar for chip processing in North America. The results indicate that this breeding approach can be used to select parents for late blight resistance breeding and to identify superior clones with high levels of late blight resistance and marketable vine maturity and tuber quality. This revised version was published online in July 2006 with corrections to the Cover Date.     

Editorial

Whether or not fruit buds in orchards can be protected from freezing by the use of fans, when no temperature inversion exists, depends upon the degree of bud radiant cooling at such times. Nighttime bud cooling in apple and peach orchards was estimated by comparing the temperature of buds and the adjacent air. The extent of radiant cooling was usually less than 0.6°C below ambient. Even this modest degree of radiant bud cooling appeared to require conditions with less than 60% cloud cover and wind speed less than 1 to 1.5 m s^?1 at a height of 2 m. However, such conditions were also accompanied by inversions. The protective potential of fans in the absence of an inversion (postulated on the basis of bud radiant cooling) is therefore disclaimed on two accounts: (1) that the bud/air temperature difference is quite small and would only rarely span the bud critical temperature, and (2) that bud cooling would probably not occur during conditions which prevent the presence of an inversion.     

Observed flow compensation associated with the MOC at 26.5 degrees N in the Atlantic

The Atlantic meridional overturning circulation (MOC), which provides one-quarter of the global meridional heat transport, is composed of a number of separate flow components. How changes in the strength of each of those components may affect that of the others has been unclear because of a lack of adequate data. We continuously observed the MOC at 26.5 degrees N for 1 year using end-point measurements of density, bottom pressure, and ocean currents; cable measurements across the Straits of Florida; and wind stress. The different transport components largely compensate for each other, thus confirming the validity of our monitoring approach. The MOC varied over the period of observation by +/-5.7 x 10(6) cubic meters per second, with density-inferred and wind-driven transports contributing equally to it. We find evidence for depth-independent compensation for the wind-driven surface flow.     

Effect of planting depth and hilling practices on total, U.S. No. 1, and field greening tuber yields

Previous research suggests that field greening of potato tubers can be minimized by planting seedpieces at an appropriate depth along with sufficient hilling to minimize exposure to sunlight. The appropriate planting depth and hilling practices to minimize field tuber greening have not been determined for newer cultivars. Two separate studies, each conducted for three years, are reported here. The first measured the effect of seedpiece planting depth on the yield, quality, and field greening tuber yield of ‘Russet Burbank’, ‘Frontier Russet’, and ‘Shepody’ potatoes. Seedpieces were handplanted in pre-formed hills at 8, 15, or 23 cm measured from the top of the seedpiece to the top of the hill. Planting at 23 cm resulted in significantly lower total yield compared with the 8- and 15-cm depths for Russet Burbank and Frontier Russet, but planting depth did not affect total yield of Shepody. U.S. No. 1 yield of Russet Burbank was not affected by planting depth, but U.S. No. 1 yield of Frontier Russet was significantly less at the 23-cm depth. For Shepody, the 8-cm depth caused a significant reduction in U.S. No. 1 yield compared with the 15-cm depth. Field greening tuber yield of Russet Burbank was significantly less at the 23-cm planting depth compared to 8 cm. For Frontier Russet and Shepody, planting at 15 or 23 cm resulted in significantly less field tuber greening compared to the 8-cm depth. The second experiment examined the effects of planting depth and hilling practices on yield, quality, and field tuber greening of Russet Burbank and Gem Russet potatoes. Six planting depth and hilling treatment combinations were used. Seedpieces were planted at a depth of either 8 or 15 cm, then hilled to either 15 or 23 cm at emergence or after plants had formed a rosette of leaves approximately 10 cm in diameter (post-emergence hilling). At-emergence hilling treatments had no effect on total or U.S. No. 1 yields of Russet Burbank compared with the 15-cm planting depth, non-hilled control. However, all post-emergence hilling treatments significantly reduced Russet Burbank total and U.S. No. 1 yields. Planting Russet Burbank at 8 cm and hilling to 23 cm at emergence, or planting at 8 or 15 cm and hilling to 23 cm post-emergence reduced field tuber greening of Russet Burbank. The effects of planting depth and hilling on Gem Russet total and U.S. No. 1 yields were less definitive than for Russet Burbank, and no treatments significantly reduced field tuber greening yield compared with the control.     

Essential regulation of CNS angiogenesis by the orphan G protein-coupled receptor GPR124

The orphan G protein-coupled receptor (GPCR) GPR124/tumor endothelial marker 5 is highly expressed in central nervous system (CNS) endothelium. Here, we show that complete null or endothelial-specific GPR124 deletion resulted in embryonic lethality from CNS-specific angiogenesis arrest in forebrain and neural tube. Conversely, GPR124 overexpression throughout all adult vascular beds produced CNS-specific hyperproliferative vascular malformations. In vivo, GPR124 functioned cell-autonomously in endothelium to regulate sprouting, migration, and developmental expression of the blood-brain barrier marker Glut1, whereas in vitro, GPR124 mediated Cdc42-dependent directional migration to forebrain-derived, vascular endothelial growth factor-independent cues. Our results demonstrate CNS-specific angiogenesis regulation by an endothelial receptor and illuminate functions of the poorly understood adhesion GPCR subfamily. Further, the functional tropism of GPR124 marks this receptor as a therapeutic target for CNS-related vascular pathologies.     

High-flux solar-driven thermochemical dissociation of CO2 and H2O using nonstoichiometric ceria

Because solar energy is available in large excess relative to current rates of energy consumption, effective conversion of this renewable yet intermittent resource into a transportable and dispatchable chemical fuel may ensure the goal of a sustainable energy future. However, low conversion efficiencies, particularly with CO(2) reduction, as well as utilization of precious materials have limited the practical generation of solar fuels. By using a solar cavity-receiver reactor, we combined the oxygen uptake and release capacity of cerium oxide and facile catalysis at elevated temperatures to thermochemically dissociate CO(2) and H(2)O, yielding CO and H(2), respectively. Stable and rapid generation of fuel was demonstrated over 500 cycles. Solar-to-fuel efficiencies of 0.7 to 0.8% were achieved and shown to be largely limited by the system scale and design rather than by chemistry.