The use of a comparative particle size distribution index for the numerical classification of soil parent materials: application to mollisols of the argentinian pampa

A method to estimate the magnitude of discontinuities or similarities in parent materials having equivalent mineralogical composition is proposed. It is based on a particle size distribution index which compares for two samples the weight percentages of all fractions taken between selected size limits.The indexes form a matrix and are used to construct a dendrogram which illustrates the clustering into groups. The calculations are carried out by computer using a Fortran programme.The technique was applied to a set of soil samples of Mollisols in the humid undulating pampa in Argentina and good agreement with field observations was obtained.     

Calculation of hydraulic conductivities of horizons in some major soils in Wisconsin

Hydraulic conductivity between saturation and a tension of 100 cm water was calculated with moisture-retention data for nine soil horizons and compared with results from in situ measurements with the crust test. Agreement was good for sandy, apedal soil horizons with simple packing voids but only if matching factors were used. Results were unreliable in clayey, pedal soil horizons in which a few relatively large planar and tubular pores determine K in the measured tension range, whereas the greatest fraction of total porosity is composed of fine pores inside peds that hardly contribute to flow. Varying the number of pore classes (n) and the water-filled porosity at saturation made no significant difference in the calculations for the apedal soils, but drastically changed the shape of the calculated curves for the pedal soils. Matching factors based on K_sat measurement had to be used for all studied soil horizons, indicating that Marshall's pore-interaction model never predicted K_sat accurately.     

Remarks on the anatomy and ontogeny of scales of teleosts. I

Densitometer records were made from the scales of a number of fish species, mainly from pikeperch. Analysis of the records showed that the pattern of hollows, representing the circuli in the curves, was very regular. No arrangement into groups of narrowly interspaced circuli could be demonstrated. It was therefore concluded that the circuli of the surface layer do not produce the “growth-checks”. Ringlike structures were also present in the fibrous basal plate. These were about 3–5 μm closer together than the circuli. These two circular gratings of slightly different inter-ring spacing will create an interference pattern, in this case a circular moiré pattern, having no direct relation to the structures present in the two layers forming the scale.     

Autolysis of chlamydospores of Fusarium solani f. sp. cucurbitae in chitin and laminarin amended soils

Lysis of chlamydospores of Fusarium solani f. sp. cucurbitae is enhanced both in chitin and in laminarin amended soils. In soil amended with both chitin and laminarin, lysis of chlamydospores resembles that in control soil. Addition of chitin and laminarin to soil stimulates the growth of both bacteria and actinomycetes. In soils amended with chitin and with both chitin and laminarin, a chitinolytic microflora is stimulated. Penetration of chlamydospore cells by soil microorganisms has not been observed using the electron microscope. The mechanism of lysis of chlamydospores in soil is discussed.     

High yield and rapid growth of Neoparamoeba pemaquidensis in co-culture with a rainbow trout gill-derived cell line RTgill-W1

Neoparamoeba pemaquidensis is an ubiquitous amphizoic marine protozoan and has been implicated as the causative agent for several diseases in marine organisms, most notably amoebic gill disease (AGD) in Atlantic salmon. Despite several reports on the pathology of AGD, relatively little is known about the protozoan and its relationship to host cells. In this study, an in vitro approach using monolayers of a rainbow trout gill cell line (RTgill-W1, ATCC CRL-2523) was used to rapidly grow large numbers of N. pemaquidensis (ATCC 50172) and investigate cell-pathogen interactions. Established cell lines derived from other tissues of rainbow trout and other fish species were also evaluated for amoeba growth support. The amoebae showed preference and highest yield when grown with RTgill-W1 over nine other tested fish cell lines. Amoeba yields could reach as high as 5 x 10(5) cells mL(-1) within 3 days of growth on the gill cell monolayers. The amoebae caused visible focal lesions in RTgill-W1 monolayers within 24 h of exposure and rapidly proliferated and spread with cytopathic effects destroying the neighbouring pavement-like cells within 48-72 h after initial exposure in media above 700 mOsm kg(-1). Disruption of the integrity of the gill cell monolayers could be noted within 30 min of exposure to the amoeba suspensions by changes in transepithelial resistance (TER) compared with control cell monolayers maintained in the exposure media. This was significantly different by 2 h (P < 0.05) compared with control cells and remained significantly different (P < 0.01) for the remaining 72 h that the TER was monitored. The RTgill-W1 cell line is thus a convenient model for growing N. pemaquidensis and for studying host-pathogen interactions in AGD.     

Effects of cover crop quality and quantity on nematode-based soil food webs and nutrient cycling

Soil food webs cycle nutrients and regulate parasites and pathogens, services essential for both agricultural productivity and ecosystem health. Nematodes provide useful indicators of soil food web dynamics. This study was conducted to determine if nematode soil food web indicators and crop yield can be enhanced by combinations of cover crops in a conservation tillage system. The effects of three cover crop treatments (vetch/pea, oat/wheat and oat/wheat/pea/vetch) with low, medium and high C:N and a bare fallow control were investigated in Davis, CA. Nematode fauna, soil properties and plant productivity were measured. Soil food web indices, including the Enrichment Index (EI), Structure Index (SI), Basal Index (BI), and Channel Index (CI), based on the composition of nematode assemblages, were calculated to infer soil food web condition. Cover cropped tomato/corn rotations had twice the number of enrichment opportunist bacterial feeding nematodes, active participants in nitrogen mineralization, than fallowed tomato/corn rotations (opportunist bacterial feeders = 163 versus 98). In winter fallowed plots food webs were basal, common in disturbed, nutrient-poor conditions (BI = 37). Total number of enrichment opportunist nematodes, soil NH₄-N levels, and inferred nitrogen mineralization, were higher in cover crop treatments with low to mid C:N ratios. Omnivore and predator nematodes were scarce, averaging less than 6 nematodes 100 g^?1 in all treatments. In year one, plant productivity was highest after fallow. In contrast, in year two productivity was highest after cover crops with high nitrogen content and productivity significantly correlated with the structure of the soil fauna. Monitoring the abundance of enrichment opportunists may provide managers with a new tool to evaluate soil food web nitrogen mineralization and plant productivity.     

Determining soil organic carbon for agricultural soils: a comparison between the Walkley & Black and the dry combustion methods (north Belgium)

Mapping soil organic carbon (SOC) and establishing any change over time are important because of CO₂ fluxes between soil and atmosphere and cropland decreases in SOC. The latter is one of the main causes of soil fertility decline and increased erodibility. As most analytical methods underestimate total SOC content, correction factors are needed to avoid methodological bias when comparing SOC data from sampling campaigns using different analytical procedures. The traditional method for SOC analysis used to be, and in most cases still is wet oxidation in potassium dichromate, better known as the Walkley & Black method. In this study, we aim to estimate correction factors for the classic and modified version of the Walkley & Black method for different land use and soil type combinations for agricultural soils in north Belgium. General correction factors of 1.47 for the classic Walkley & Black method and 1.20 for the modified Walkley & Black method are proposed. The results show that sandy grassland soils are characterised by lower recoveries than silt loam grassland soils. Furthermore, the correction factor appears to increase with soil wetness.     

Chronology of plaggic deposits; palynology,radiocarbon and optically stimulated luminescence dating of the Posteles (NE-Netherlands)

Plaggic agriculture was the dominant land use system on Pleistocene sandy soils in Northwest Europe before the introduction of chemical fertilizers. In the today's landscape plaggic deposits are still recognizable as characteristic soils and landforms, related to this historical land use system. They are also important constituents of the soil archives in Northwest Europe. In preliminary studies of plaggic anthrosols, the reconstruction of the development of the plaggic deposits was based on palynological and historical data and radiocarbon dates. Disagreement appeared between palaeopedological and historical explanation of the evolution of plaggic anthrosols. Application of OSL dating improves the knowledge of chronology of plaggic deposits. The soil organic matrix, including pollen grains, consists of a complex mix of compounds of different ages and sources. Pollen diagrams of plaggic anthrosols show a palynological registration of the Late Holocene landscape evolution, but the palynological signal is not consequently correlated with the age of the plaggic sediments. An older soil organic matrix is suspended in the voids of a younger mineral soil skeleton.