COMPARISON OF PER-RECTAL PORTAL SCINTIGRAPHY USING 99mTECHNETIUM PERTECHNETATE TO MESENTERIC INJECTION OF RADIOACTIVE MICROSPHERES FOR QUANTIFICATION OF PORTOSYSTEMIC SHUNTS IN AN EXPERIMENTAL DOG MODEL

Per-rectal portal scintigraphy using tech-netium-99m pertechnetate (^99mTcO₄^-) was performed in 8 normal dogs before and after surgical creation of a portacaval shunt. Shunt fractions were calculated by computer assisted analysis of dynamic images (IMG) and compared to shunt fractions determined by mesenteric venous injection of radioactive microspheres (MIC). The mean pre-operative shunt fraction was 1.59% using IMG and 3.00% using MCI. The mean postoperative shunt fraction was 64.56% using IMG and 69.56% using MIC. There was excellent correlation between both methods (r² 0.94). Per-rectal portal scintigraphy is an easily performed, inexpensive method to diagnose and quantify portosystemic shunts in dogs.     

Current knowledge and breeding perspectives for the spider plant (<Emphasis Type="Italic">Cleome gynandra</Emphasis> L.): a potential for enhanced breeding of the plant in Africa

Cleome gynandra is one of the most promising African leafy vegetables consumed in most parts of Africa. The crop is a rich source of vitamins A and C and minerals such as calcium, iron, magnesium and protein. The leaves and seeds of spider plant are used in indigenous medicine in several countries. Despite the numerous nutritive benefits and ethnopharmacological uses of the plant, C. gynandra is still underutilized due to lack of awareness and promotion of production technologies and utilization. Available information is limited and short on several relevant and rudimentary components. Hence, this review focuses on the current knowledge on C. gynandra, with regards to its botany, ecology, cultivation systems, utilization, nutraceutical properties as well as the breeding avenues that will contribute to promoting the species, aimed at ensuring food and nutritional security and efficient management of its genetic resources. Breeding programmes aimed at minimizing the bitterness of the leaves so to arouse the interest of consumers, and the mechanisms involved in the responses and adaptation of C. gynandra as a C₄ plant to salinity stress, were also explored.     

Estrus Synchronization in Sheep and Goats Using Combinations of GnRH,Progestagen and Prostaglandin F2α

The objective of this experiment was to evaluate the effect of GnRH, progestagen and prostaglandin F_2α on estrus synchronization in sheep and goats. Sixty Awassi ewes and 53 Damascus does were used in the study. The experiment started at the beginning of the breeding season (June/July). The same treatments were applied to sheep and goats as follows: no treatment (CON), 14‐day progestagen sponges and 600 IU equine chorionic gonadotropin (S), gonadotropin releasing hormone followed 5 days later by prostaglandin F_2α (GP) and gonadotropin releasing hormone, progestagen sponges for 5 days and prostaglandin F_2α on the day of sponge removal (GSP). None of the ewes in the S group lambed from mating during the induced cycle. A greater lambing rate (p < 0.05) was observed in the GSP group compared with the CON and S groups while the GP group was intermediate. The number of lambs born per lambed ewe was similar among the CON, GP and GSP groups. However, the number of lambs per exposed ewe was greater (p < 0.05) in the GSP than the remaining groups. The induced cycle kidding rate was 77% for all treatments combined. Similar kidding rate were observed among treatments. The numbers of kids born per kidded and exposed doe from mating during the induced estrus were also similar among treatments. Greater numbers of multiple births were observed in the GP and GSP than in the S group. In conclusion, a combination of GnRH, progestagen sponges and PGF_2α can be effective in synchronizing estrus and improving fecundity in sheep and goats. Although the use of GnRH–PGF_2α was effective, the addition of progestagen sponges at the time of GnRH administration appeared to improve reproductive parameters.     

The structure of the upper atmosphere of mars: In situ accelerometer measurements from mars global surveyor

The Mars Global Surveyor (MGS) z-axis accelerometer has obtained over 200 vertical structures of thermospheric density, temperature, and pressure, ranging from 110 to 170 kilometers, compared to only three previous such vertical structures. In November 1997, a regional dust storm in the Southern Hemisphere triggered an unexpectedly large thermospheric response at mid-northern latitudes, increasing the altitude of thermospheric pressure surfaces there by as much as 8 kilometers and indicating a strong global thermospheric response to a regional dust storm. Throughout the MGS mission, thermospheric density bulges have been detected on opposite sides of the planet near 90 degreesE and 90 degreesW, in the vicinity of maximum terrain heights. This wave 2 pattern may be caused by topographically-forced planetary waves propagating up from the lower atmosphere.     

Linking winter and summer events in a migratory bird by using stable-carbon isotopes

For migratory birds, early arrival and physical condition on the breeding grounds are important determinants of reproductive success and fitness. Differences in arrival times often exceed a month, and later arriving individuals are often in poorer condition. Habitat-specific isotopic signatures indicate that the quality of winter habitats occupied by American redstarts (Setophaga ruticilla) determines their physical condition and spring departure dates, which in turn result in variable arrival schedules and condition on temperate breeding grounds. These findings link events in tropical winter grounds with those in temperate breeding areas for a migratory songbird and provide evidence that winter habitats may be limiting.     

A REVIEW OF HIGH RESOLUTION COMPUTED TOMOGRAPHY AND A PROPOSED TECHNIQUE FOR REGIONAL EXAMINATION OF THE CANINE LUMBOSACRAL SPINE

High resolution computed tomography (CT) is a noninvasive imaging modality that has been used extensively in evaluating diseases of the human lumbosacral spine. Excellent spatial and contrast resolution, combined with multiplanar reformatting capability make high resolution CT scanners well-suited for similar applications in dogs. Consistently good quality images can be obtained when careful attention is given to factors affecting resolution. This paper reviews and illustrates some principles of high resolution CT, and proposes a technique for regional CT examination of the canine lumbosacral spine.     

MAGNETIC RESONANCE IMAGING—A GENERAL OVERVIEW OF PRINCIPLES AND EXAMPLES IN VETERINARY NEURODIAGNOSIS

Magnetic resonance imaging (MRI), a noninvasive technique that provides accurate, detailed, anatomic images, has had a major impact in the diagnosis of human disease. This technique is based upon the inherent magnetic properties of certain nuclei. Induction of the nuclei into a low energy state is achieved by placing them in a static magnetic field. The nuclei may then be excited into a high energy state by application of a radio frequency pulse. When the second field is stopped, the nuclei return to ground state and emit the absorbed energy in the form of a radio signal. This signal is received by a coil that generally surrounds the specimen and converted to an anatomic image through a process of computer-assisted reconstruction. Contrast is altered by applying the second pulse in different sequences (saturation recovery, inversion recovery, and spin echo) and using enhancing agents such as gadolinium. In this paper, we present an overview of the general principles of MRI and some clinical examples in dogs and cats with central nervous system disease.     

Leptospira interrogans serovar hardjo is not a major cause of bovine abortion in Victoria

The aim of this study was to determine whether evidence could be obtained of foetal infection with Leptospira interrogans serovar hardjo in aborted foetuses collected from dairy farms. Material from 197 abortions occurring over a wide area of Victoria was collected over 3 years. None of 195 foetal kidney cultures or 7 cultures from membranes was positive for leptospiral organisms. Immunogold silver staining for leptospires was performed on sections of kidneys, lungs or heart from 156 foetuses, with negative results. Evidence of transient leptospiral infection in 11 of 123 foetuses was obtained by foetal heart blood serology. Two isolates of L. interrogans serovar hardjo were obtained from the urine of milking cows. These strains were examined by restriction endonuclease analysis and both were shown to be of the genotype Hardjobovis, as have been all Australian isolates studied so far. It appears that foetal infection with serovar hardjo is not associated with any substantial proportion of bovine abortions in Victoria, in contrast to the situation in Northern Ireland. The apparent absence from Victoria of the pathogenic genotype Hardjoprajitno is a possible explanation.