桑悬浮细胞原生质体培养的研究

采用继代培养三个月后的桑子叶悬浮细胞为材料,进行了原生质体的分离和培养。桑悬浮细胞在纤维素酶、果胶酶、半纤维素酶的混合溶液中,酶解获得产量高、活力强的原生质体。原生质体在K8p(附加6—BA、NAA、2,4—D、LH)液体培养基中,再生细胞经多次分裂,得到肉眼可见的小愈伤组织。再通过增殖继代培养,获得浅黄色、具有明显颗粒结构的愈伤组织,转至各种激素含量的MSB固体培养基中,尚未获得绿苗分化。     

饲粮不同蛋白质、能量水平对大白鼠生长发育及体成分的影响

本试验所用动物为53—57日龄雄性大白鼠(sprague——Dawley品系),初始体重为121.73±3.95g,试验期为大白鼠生长发育旺期的21天。试验拟研究不同粗蛋白水平(10％、15％、20％、25％和30％)及两个能量水平(消化能:3.6和3.9kcal/g)的饲粮对大白鼠生长发育及各种体成分的影响。结果表明,当饲粮蛋白质供应充分时,大白鼠生长随饲粮能量浓度的提高而改善;而当饲粮蛋白质供应感觉不足时,提高能量浓度对生长无益。机体各项成分指杯中,以蛋白质、脂肪、水分沉积受饲粮蛋白质,能量浓度影响显著;而蛋白质、脂肪沉积在很大程度上受饲粮能朊比的影响,饲粮中添加脂肪可使大白鼠机体肥胖。     

柠檬酸废液深层培养双孢蘑菇菌丝蛋白的研究

柠檬酸工业废液中含有一定有效物质,如糖类、蛋白质、矿物质等,可供微生物利用。本文介绍了利用废液深层培养双孢蘑菇菌丝蛋白的可能性及条件。并用1吨发酵堆进行生产试验,探讨了生产工艺条件,分析了发酵液中底物、产物的动态变化。双孢蘑菇菌丝蛋白培养温度25℃,底物 pH6,发酵周期6天,菌球直径1～3mm,菌球数量4.8×10~3个/ml,菌丝温重达底物量的29.3%。在上述条件下,发酵液中菌球直经、菌丝数量、发酵液粘度受生物剪切作用的影响,粘度大,剪切作用大,菌球直径小,菌球数量则多。本文为新型单细胞蛋白的开发,促进物质的多层次利用,消除环境污染,提供了一个新途径。     

Investigation of Cervical Patency and Uterine Appearance in Domestic Cats by Fluoroscopy and Scintigraphy

The cervical patency of six domestic female cats was monitored under sedation by infusion of contrast medium (Omnipaque) into the cranial vagina during early oestrus, mid‐oestrus, late oestrus and interoestrus or a radiopharmaceutical (^99mTc‐HSA) during mid‐ and interoestrus in a non‐ovulatory oestrous cycle. The transport of the contrast medium or the radiopharmaceutical through the cervix and within the uterine horns was observed under fluoroscopy and with the aid of scintigraphy. In three of the queens, transcervical transport of contrast medium was demonstrated in all stages of oestrus, in one queen during mid‐oestrus, late oestrus and 1 day after oestrus, and in two queens only during late oestrus. The relations between the cervical patency to the contrast medium and the oestrous behaviour, cornification of the vaginal cells and the serum oestradiol‐17β concentration were evaluated, and a relationship was found between the cervical patency and the degree of vaginal cornification. Transcervical transport of the radiopharmaceutical was observed in three queens during mid‐oestrus. When the cervix was open, hysterography under a fluoroscope and hysteroscintigraphy were performed. The fluoroscopic and scintigraphic recordings revealed the patterns of the uterine contractions during oestrus in both ascending and descending directions, and the movement of the uterine contents back and forth between the uterine horns. The hysterograms were classified according to the shape of the uterine horns and the appearance of the endometrial lining. Spiral‐shaped uterine horns with a smooth inner contour were observed in two queens, and a corkscrew appearance with irregular filling defects in the uterine lumen was shown in two queens that had developed subclinical cystic endometrial hyperplasia. These findings demonstrated that fluids or particles deposited in the cranial vagina of the cat can be transported into the uterus during some stages of the oestrous cycle. The fluoroscopic and scintigraphic techniques developed in this study may be further modified to permit more detailed studies of uterine contractile patterns and sperm transport in the feline female reproductive tract. Hysterography proved useful to diagnose uterine disease. The information on cervical patency is of value also for the development of techniques for artificial insemination in this species, and should be studied also in the ovulatory cycle.     

控制sch单养雄蚕品种雌雄蚕孵出比例的催青处理因素研究

研究结果表明 :在RH 6 0 %状态下 ,控制性比的温度和下限时间负相关达到显著水平 ;控制性比的高温处理适于干燥状态下进行 ;处理后雌蚕孵化整齐度明显低于雄蚕孵化整齐度 ,雌雄蚕孵化整齐度均随处理温度的升高而下降 ,尤以雌蚕更为明显。由此提出了在RH 6 0 %状态下sch单养雄蚕品种控制性比的起点温度及温度、时间范围。认为高温催青用下限温度配合 1日收蚁法效果好 ,高温处理以后的胚胎适于在RH 80 %～ 85 %的湿度中保护     

畜牧业生产中的环境污染及治理对策

本文阐述了畜牧业生产中产生的畜禽粪尿、有机废水等废弃物对环境造成的污染，探讨了治理畜牧业污染的措施，提出了发展生态畜牧业的思路。     

盐胁迫对4种禾本科牧草种子萌发和幼苗生长的影响

通过NaCl溶液(0、1.5、3.0、6.0、9.0g/L)处理4种禾本科牧草种子,测定了种子萌发和幼苗生长有关指标。结果表明:美洲狼尾草较耐盐,在9.0g/L盐溶液处理下比其它品种维持了高的发芽率、发芽指数、活力指数,并且其相对值高,以及较高的相对苗长和根长,盐处理显著提高几种牧草幼苗质膜相对透性,总体上降低胚乳的利用速度和利用效率。     

厦门市生物多样性的保护措施探讨

叙述厦门市生物多样性现状，分析当前生物多样性保护中存在的主要问题，并提出应采取的对策措施。     

MapInfo在基准地价评估成果图中的应用实践

本文以MapInfo7.5作为GIS的平台软件,将MapInfo用于基准地价评估成果图的信息管理,利用创建、修改或更新的相关表格,建立相应的专题地图,使成果图中的信息更加直观明了。实践证明,MapInfo为基准地价评估成果图的及时更新、科学管理、有效利用提供了可靠的途经。     

南非糖业的技术特点及几项技术革新项目简介

南非糖业的生产成本较低，原糖质量高，该国普遍采用渗出法提汁和连续结晶罐，改进清净工艺和煮糖制度生产高纯度原糖。文中并介绍蒸汽喷射抽真空、内置冷凝器、高纯度原糖工艺、精炼粉糖、渗出法提汁和气垫胶带运输机等技术革新项目。