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排序方式: 共有498条查询结果,搜索用时 15 毫秒
31.
Stephanie M Nowacek Randall S Wells Edward C.G Owen Richard O Flamm 《Biological conservation》2004,119(4):517-523
Florida manatees inhabit shallow coastal and estuarine waters of the southeast US, a range that brings them into frequent contact with vessels. More than 30% of documented annual mortalities are attributed to vessel collisions, and most living animals bear the scars of multiple, non-lethal encounters. To document the behavior of manatees in the presence of vessels, we recorded their movements with an overhead video system. We scored six aspects of behavior during 170 vessel approaches, and compared their behavior with 187 control segments when no boats were present. Manatees in shallow waters and at the edge of the channel responded to approaches by orienting towards the nearest deep water, a boat channel, and increasing their swimming speed. Close boat approaches and shallow water depths exacerbated these responses. Our results indicate that manatees detect and respond to approaching vessels with an apparent flight response, a response which includes movement towards deeper water. If given sufficient time, i.e., approached or passed slowly, the manatees may then be able to reach deeper water and safe depths. 相似文献
32.
Stabilization of Z DNA in vivo by localized supercoiling 总被引:40,自引:0,他引:40
33.
Engineering enzyme specificity by "substrate-assisted catalysis" 总被引:8,自引:0,他引:8
A novel approach to engineering enzyme specificity is presented in which a catalytic group from an enzyme is first removed by site-directed mutagenesis causing inactivation. Activity is then partially restored by substrates containing the missing catalytic functional group. Replacement of the catalytic His with Ala in the Bacillus amyloliquefaciens subtilisin gene (the mutant is designated His64Ala) by site-directed mutagenesis reduces the catalytic efficiency (kcat/Km) by a factor of a million when assayed with N-succinyl-L-Phe-L-Ala-L-Ala-L-Phe-p-nitroanilide (sFAAF-pNA). Model building studies showed that a His side chain at the P2 position of a substrate bound at the active site of subtilisin could be virtually superimposed on the catalytic His side chain of this serine protease. Accordingly, the His64Ala mutant hydrolyzes a His P2 substrate (sFAHF-pNA) up to 400 times faster than a homologous Ala P2 or Gln P2 substrate (sFAAF-pNA or sFAQF-pNA) at pH 8.0. In contrast, the wild-type enzyme hydrolyzes these three substrates with similar catalytic efficiencies. Additional data from substrate-dependent pH profiles and hydrolysis of large polypeptides indicate that the His64Ala mutant enzyme can recover partially the function of the lost catalytic histidine from a His P2 side chain on the substrate. Such "substrate-assisted catalysis" provides a new basis for engineering enzymes with very narrow and potentially useful substrate specificities. These studies also suggest a possible functional intermediate in the evolution of the catalytic triad of serine proteases. 相似文献
34.
KR Arrigo DL Worthen MP Lizotte P Dixon G Dieckmann 《Science (New York, N.Y.)》1997,276(5311):394-397
A numerical model shows that in Antarctic sea ice, increased flooding in regions with thick snow cover enhances primary production in the infiltration (surface) layer. Productivity in the freeboard (sea level) layer is also determined by sea ice porosity, which varies with temperature. Spatial and temporal variation in snow thickness and the proportion of first-year ice thus determine regional differences in sea ice primary production. Model results show that of the 40 teragrams of carbon produced annually in the Antarctic ice pack, 75 percent was associated with first-year ice and nearly 50 percent was produced in the Weddell Sea. 相似文献
35.
Yellow nutsedge (Cyperus esculentus L.) is a serious weed problem in the United States and other countries. An indigenous rust fungus [Puccinia canaliculata (Schw.) Lagerh.], pathogenic on yellow nutsedge, was released in early spring as a potential biological control agent. The fungus inhibited nutsedge flowering and new tuber formation. The fungus also dehydrated and killed nutsedge plants. The successful control of yellow nutsedge by a rust epiphytotic under experimental conditions demonstrates the potential use of the rust in an integrated weed management system. 相似文献
36.
Galibert F Finan TM Long SR Puhler A Abola P Ampe F Barloy-Hubler F Barnett MJ Becker A Boistard P Bothe G Boutry M Bowser L Buhrmester J Cadieu E Capela D Chain P Cowie A Davis RW Dreano S Federspiel NA Fisher RF Gloux S Godrie T Goffeau A Golding B Gouzy J Gurjal M Hernandez-Lucas I Hong A Huizar L Hyman RW Jones T Kahn D Kahn ML Kalman S Keating DH Kiss E Komp C Lelaure V Masuy D Palm C Peck MC Pohl TM Portetelle D Purnelle B Ramsperger U Surzycki R Thebault P Vandenbol M Vorholter FJ 《Science (New York, N.Y.)》2001,293(5530):668-672
The scarcity of usable nitrogen frequently limits plant growth. A tight metabolic association with rhizobial bacteria allows legumes to obtain nitrogen compounds by bacterial reduction of dinitrogen (N2) to ammonium (NH4+). We present here the annotated DNA sequence of the alpha-proteobacterium Sinorhizobium meliloti, the symbiont of alfalfa. The tripartite 6.7-megabase (Mb) genome comprises a 3.65-Mb chromosome, and 1.35-Mb pSymA and 1.68-Mb pSymB megaplasmids. Genome sequence analysis indicates that all three elements contribute, in varying degrees, to symbiosis and reveals how this genome may have emerged during evolution. The genome sequence will be useful in understanding the dynamics of interkingdom associations and of life in soil environments. 相似文献
37.
G.?VellidisEmail author M.?A.?Tucker C.?D.?Perry D.?L.?Thomas N.?Wells C.?K.?Kvien 《Precision Agriculture》2004,5(6):547-564
It is generally accepted that aerial images of growing crops provide spatial and temporal information about crop growth conditions and may even be indicative of crop yield. The focus of this study was to develop a straightforward technique for creating predictive cotton yield maps from aerial images. A total of ten fields in southern Georgia, USA, were studied during three growing seasons. Conventional (true color) aerial photographs of the fields were acquired during the growing season in two to four week intervals. The aerial photos were then digitized and analyzed using an unsupervised classification function of image analysis software. During harvest, conventional yield maps were created for each of the fields using a cotton picker mounted yield monitor. Classified images and yield maps were compared quantitatively and qualitatively. A pixel by pixel comparison of the classified images and yield maps showed that spatial agreement between the two gradually increased in the weeks after planting, maintained spatial agreement of between 40% and 60% during weeks eight to fourteen, and then gradually declined again. The highest spatial agreement between a classified image and a yield map was 78%. The highest average agreement was 52% and occurred 9.9 weeks after planting. The visual similarity between the classified images and the yield maps were striking. In all cases, the dates with the best visual agreement occurred between eight and ten weeks after planting, and generally, during July for southern Georgia. This method offers great potential for offering cotton farmers early-season maps that predict the spatial distribution of yield. Although these maps can not provide magnitudes, they clearly show the resulting yield patterns. With inherent knowledge of past performance, farmers can use this information to allocate resources, address crop growth problems, and, perhaps, improve the profitability of their farm operation. These maps are well suited to be offered to farmers as a service by a crop consultant or a cooperative. 相似文献
38.
Galactose-fed chicks have been found to develop severe hyperosmolar dehydration. Although biochemical abnormalities have been observed in the brain of the galactose-toxic chick, the observed physiologic alteration of serum osmolality could be the major factor responsible for the galactose toxicity syndrome in the chick. 相似文献
39.
Wells PV 《Science (New York, N.Y.)》1970,167(3925):1574-1582
40.
Wells RV 《Science (New York, N.Y.)》1988,242(4877):448-450