Einfluß von Herbiziden auf die N2-Fixierung und Atmungsaktivität von Mikroorganismen in Ackerböden

Influence of herbicides on nitrogen fixation and respiration activity of microorganisms in arable soils The influence of pesticides on nitrogen fixation (acetylene reduction test) as well on respiration activity was determined in model experiments. The following soils were used: Chernozem from loess (Boroll), Luvisol from loess (Boralfs), Rendsina (Lithic Rendoll), Pelosol (Fine textured Cambisol) and Humic Podzol (Humod). The tested soils differed considerably in both parameters. The rendsina showed remarkably low fixation rates whereas the podsol reduced acetylene only at higher water contents. The soil herbicides chlortoluron, terbutryne, metabenzthiazuron and chloridazon did not affect the course and the magnitude of the tested parameters even not at higher doses. Only the leaf herbicide dinosebacetate revealed a distinct inhibition of nitrogenase activity in the podsol and in the luvisol from loess. The fungicide carbendazime caused a strong stimulation of the nitrogenase activity in all soils. The respiration activity could not been influenced significantly.     

A microcosm study on the influence of pH and the host-plant on the soil persistence of two alfalfa-nodulating rhizobia with different saprophytic and symbiotic characteristics

The acid tolerance of Sinorhizobium meliloti in culture media and in soils is considered a useful criteria to select for strains with improved survival in agricultural acidic soils. Using a glass tube system with gamma-irradiated soil at different pH values, we analysed the survival of two different alfalfa-nodulating rhizobia: S. meliloti (pH_limit for growth 5.6–6.0) and the acid-tolerant Rhizobium sp. LPU83, closely related to the strain Rhizobium sp. Or191 (pH_limit for growth below 5.0). Although the acid-tolerant rhizobia showed a slightly better survival during the first months in acid soil (pH=5.6), none of the strains could be detected 2 months after inoculation (bacterial counts were below 10³ colony-forming units (cfu)/30 g of soil). The inclusion of two alfalfa plants/glass tube with soil, however, supported the persistence of both types of rhizobia at pH 5.6 for over 2 months with counts higher than 9×10⁶ cfu/30 g of soil. Remarkably, in the presence of alfalfa the cell densities reached by S. meliloti were higher than those reached by strain LPU83, which started to decline 1 week after inoculation. Although more acid-sensitive in the culture medium than the Or191-like rhizobia, in the presence of the host plant the S. meliloti strains showed to be better adapted to the free-living condition, irrespective of the pH of the soil.     

The role of sediment-feeding oligochaeteTubifex on the availability of trace metals in sediment pore waters as determined by diffusive gradients in thin films (DGT)

Background  Available fractions of metal concentrations in sediment pore water are difficult to measure without disturbing the sediment core. The available fraction is an important parameter in understanding the risks for benthic organisms. Not much is known about the influence of the presence of benthic organisms to the available fraction. Objectives  The aim of this study was to discuss the dynamics of metal behaviour in natural sediments and the influence of the presence ofTubifex on sediment chemistry using DGT. Methods. DGT-probes were added to six beakers to cover a depth profile of 0 to 13 cm. The six beakers contained three different sediments. For each sediment, one beaker had 1 g ofTubifex added, the other beaker contained noTubifex. After two weeks of exposure, the probes were withdrawn and strips were analysed for their content of cadmium, copper and zinc. Results and discussion  Available concentrations were higher in the upper layer (0–1 cm) of the sediment core, where conditions are generally more oxic. The presence ofTubifex worms led to a decrease of the available concentrations in the upper layer, due to the competition of the worms with the DGT for the available metal fraction. On the other hand, the presence of the worms led, via bioturbation, to an increased oxygen penetration depth in the sediment, and, as a consequence, to an increase of the available fraction in the next-higher sediment layer. Conclusions  In sediment cores without organisms, very steep gradients of the available metal concentration in pore water have been found. The change from a high to low amount of available fraction corresponds to the change from oxidised conditions in the surficial sediment layer to reduced conditions in the lower sediment layers. The tunnelling behaviour ofTubifex worms leads to a penetration of oxygen to deeper sediment layers, and to an increase of the available metal fraction in the pore water.Tubifex competes with DGT for the available fraction, resulting in lower metal amounts accumulated in the DGT probe. Competition is the prevailing effect in the top 1 cm layer of all sediments. In the second 1 cm layer, competition, in most cases, is not strong enough to compensate the increase in metal availability due to the broadening of the oxidised zone. Recommendations  The effects of sediment organisms on the deepening of the oxidised layer and on the availability of metals in pore water need further investigations and quantification. DGT is recommended as an instrument for measuring available metal concentrations in these studies.     

RIM-binding protein, a central part of the active zone, is essential for neurotransmitter release

The molecular machinery mediating the fusion of synaptic vesicles (SVs) at presynaptic active zone (AZ) membranes has been studied in detail, and several essential components have been identified. AZ-associated protein scaffolds are viewed as only modulatory for transmission. We discovered that Drosophila Rab3-interacting molecule (RIM)-binding protein (DRBP) is essential not only for the integrity of the AZ scaffold but also for exocytotic neurotransmitter release. Two-color stimulated emission depletion microscopy showed that DRBP surrounds the central Ca(2+) channel field. In drbp mutants, Ca(2+) channel clustering and Ca(2+) influx were impaired, and synaptic release probability was drastically reduced. Our data identify RBP family proteins as prime effectors of the AZ scaffold that are essential for the coupling of SVs, Ca(2+) channels, and the SV fusion machinery.     

Geomorphic features and soil formation of arid lands in Northeastern Jordan

Arid and semiarid lands occupy about one-third of the Earth's land surface. Interpretation of soil formation and geomorphic features of arid lands is needed to assess their soil ecological potentials, limitations, problems and management needs. The objective of this paper was to study the geomorphic features and soil formation of the arid lands in northeastern Jordan, to provide information that could be used by land managers in the study area and other arid land areas. Five representative soil pedons were excavated and described in the field. Soil samples from each horizon per pedon were taken to the laboratory for chemical and physical analyses. Geomorphic features of the area were also studied. Most of studied land surfaces are plains where eolian deflation has exposed loose gravels consisting predominantly of pebbles forming desert pavements. Desert pavements cover most of the land surface, excluding the mud playas, and are composed of basalt clasts. The accumulation of calcium carbonate and gypsum within these soils create problems for their agricultural development. Accumulation of eolian fine-grained silt has resulted in the formation of a vesicular horizon. The climatic variations during the late quaternary and the late Holocene periods contributed to the development of the desert pavement and the vesicular horizons. Clay illuviation and argillic horizon development within these soils is assumed to be a relict feature from more humid climates during the Quaternary. Sustainable agricultural development of such arid lands may not be easy. In general, these soils have high erodibility, high runoff generation potential, high susceptibility to seal and crust formation, poor water-holding capacity, pedon hardening and structural instability.     

Dedicated to the 100th birthday of Professor Dr. Dr. h. c. mult. Eduard von Boguslawski

Identifying the optimum resolution where differences in corn (Zea mays L.) grain yields are detectable could theoretically improve nitrogen (N) management, thereby resulting in economic and environmental benefits for producers and the public at large. The objective of this study was to determine the optimum resolution for prediction of corn grain yield using indirect sensor measurements. Corn rows, 15–30 m long, were randomly selected at three locations where the exact location of each plant was determined. In 2005 and 2006, four of eight rows at each location were fertilized with 150 kg N ha^?1 as urea ammonium nitrate (28% N). A GreenSeeker? optical sensor was used to determine average Normalized Difference Vegetation Index (NDVI) across a range of plants and over fixed distances (20, 40, 45.7, 60, 80, 91.4, 100, 120, 140, 160, 180, 200, 220, and 240 cm). Individual corn plants were harvested and grain yield was determined. Correlation of corn grain yield versus NDVI was evaluated over both increasing distances and increasing number of corn plants. Then, the squared correlation coefficients (r_cc ²) from each plot (used as data) were fitted to a linear plateau model for each resolution treatment (fixed distance and number of corn plants). The linear-plateau model coefficient of determination (r_lp ²) was maximized when averaged over every four plants in 2004 and 2006, and over 11 plants in 2005. Likewise, r_lp ² was maximized at a fixed distance of 95, 141, and 87 cm in 2004, 2005, and 2006, respectively. Averaged over sites and years, results from this study suggest that in order to treat spatial variability at the correct scale, the linear fixed distances should likely be <87 cm or <4 plants as an optimum resolution for detecting early-season differences in yield potential and making management decisions based on this resolution.     

Soft-rot Resistance of Coloured Potato Cultivars (<Emphasis Type="Italic">Solanum tuberosum</Emphasis> L.): The Role of Anthocyanins

Coloured potatoes (Solanum tuberosum L.) rich in anthocyanins are gaining increasing interest on the markets, mainly due to beneficial health effects. Potato varieties containing colour pigments are therefore utilized more and more in actual breeding programmes. In this study, 10 purple-fleshed potato cultivars were compared in their resistance to soft rot caused by Pectobacterium carotovorum (Pc) with 10 white/yellow-fleshed potato cultivars. The results revealed that coloured potato cultivars exhibited on average better resistance to soft rot than white/yellow-fleshed potato cultivars. On tuber halves of purple-fleshed cultivars, the extent of rotting caused by Pc was on average 28.6% lower than on white/yellow-fleshed cultivars. The greater resistance of coloured potatoes coincided with the presence of anthocyanins, higher concentrations of soluble phenols and elevated polyphenol oxidase activity in tuber tissue. These three components were significantly correlated with the extent of rotting caused by Pc. However, coloured and white/yellow-fleshed cultivars were not very different in phenylalanine ammonia-lyase and peroxidase activity. There were also no significant differences in concentrations of dry matter, starch, crude protein and glycoalkaloids. Key findings reveal that total soluble phenols and anthocyanins contributed crucially to resistance expression in tuber tissue of coloured potato cultivars.