Gibberellin and Auxin-Induced Berry Enlargement in Vitis Vinifera L.

Gibberellic acid (GA) and the auxin, 4 chlorophenoxyacetic acid (CPA), differ in their effects upon berry enlargement in two seedless varieties of Vitis vinifera L., cv. Black Corinth and Thompson Seedless. Although initially both substances cause nearly equal increases in berry growth rates, at maturity GA-treated berries far surpass those treated with CPA. The growth rate of CPA-treated berries declines, relative to that of the GA group, two to seven days after treatment. The results of experiments in which berries received treatment with both GA and CPA show that CPA may act as an inhibitor of GA-induced expansion, particularly during the later stages of enlargement. Also, GA-treated berries have a greater length-to- width ratio than CPA-treated berries. Thus, there are apparently qualitative differences in mode of action between the two substances. Increase in berry size, regardless of treatment, is the result of water uptake accompanied by solute storage and synthesis of cell components. Histological studies reveal that berry enlargement is the result of tissue growth in a region of the pericarp between the locule and peripheral vascular tissues. This tissue increases in size within 48 hours after treatment with GA or CPA. In GA- treated berries there is a 10-fold increase in parenchyma cell diameter in this tissue between anthesis and maturity. New cell formation in the pericarp of the GA-treated berries may also occur. GA has a more pronounced effect upon enlargement of distal than of proximal parenchyma tissues in the Black Corinth variety. Tissue expansion in CPA-treated berries, as well as those on girdled vines, was approximately equal at both poles. Such differential effects upon expansion at the proximal and distal poles may account for the difference in shape between GA- and CPA-treated berries.     

Brucellosis associated with stillbirth in a bottlenose dolphin in Australia

A captive adult female bottlenose dolphin presented with stillbirth. The placenta appeared oedematous. No other gross lesions were evident in the placenta or the stillborn calf. Histopathology revealed mild multifocal placentitis and foetal encephalitis. Brucella sp. was isolated from lung, liver, spleen and kidney. Sequence and phylogenetic analysis demonstrated this organism to be most similar to Brucella ceti sequence type (ST) 27. Brucella sp. DNA was detected in formalin-fixed paraffin-embedded placenta and brain by real-time PCR using primers targeting the IS711 gene. Immunohistochemical staining revealed Brucella sp. antigen in placental inflammation. This is the first report of isolation of Brucella sp. from a marine mammal in the Southern Hemisphere and the first report of marine Brucella-associated disease in Australia.     

Impacts of Dryland Cropping Systems on Ground Beetle Communities (Coleoptera: Carabidae) in the Northern Great Plains

Ground beetles are natural predators of insect pests and small seeds in agroecosystems. In semiarid cropping systems of the Northern Great Plains, there is a lack of knowledge to how ground beetles are affected by diversified cover crop rotations. In a 2-yr study (2018 and 2019), our experiment was a restricted-randomization strip-plot design, comprising summer fallow, an early-season cover crop mixture (five species), and a mid-season cover crop mixture (seven species), with three cover crop termination methods (i.e., herbicide, grazing, and haying). Using pitfall traps, we sampled ground beetles in five 48-h intervals throughout the growing season (n = 135 per year) using growing degree day (GDD) accumulations to better understand changes to ground beetle communities. Data analysis included the use of linear mixed-effects models, perMANOVA, and non-metric multidimensional scaling ordinations. We did not observe differences among cover crop termination methods; however, activity density in the early-season cover crop mixture decreased and in summer fallow increased throughout the growing season, whereas the mid-season cover crop mixture peaked in the middle of the summer. Ground beetle richness and evenness showed a nonlinear tendency, peaking in the middle of the growing season, with marginal differences between cover crops or fallow after the termination events. Also, differences in ground beetle composition were greatest in the early- and mid-season cover crop mixtures earlier in the growing season. Our study supports the use of cover crop mixtures to enhance ground beetle communities, with potential implications for pest management in dryland cropping systems.     

Effect of rate and time of fertilization on nitrogen and yield of Russet Burbank potatoes under center pivot irrigation

Applying less N on Russet Burbank potatoes (Solanum tuberosum L.) at planting time may reduce the potential loss of N from sandy soil by wind erosion and leaching early in the season. The objective of this study was to evaluate the effect of rate and time of N fertilization on potato production. Potatoes were grown in outdoor pot cultures with N rates of 75,150 and 300 ppm and in field plots with N rates from 112 to 448 kg N/ha applied in single and split applications. The results from pot cultures harvested after one month’s growth showed that yield of tops increased and tuber yield decreased as applied N at planting increased from 75 ppm to 150 or 300 ppm. Field results showed that the yield of potatoes was as high or higher when N applications were split between planting and when plants were 15 to 20 cm tall (early tuberization) as with the same amount of N applied at planting. Generally 112 kg N/ha at planting time was sufficient when additional N was applied after emergence. Split application of N resulted in more second growth on tubers than when all of the N was applied at planting.     

Study of hydrogen peroxide,potato enzymes and blackspot

The rate of oxidation of tyrosine, p-cresol and catechol by potato enzyme diminished as H₂O₂ concentration increased. By contrast, the rate of oxidation of chlorogenic acid in the presence of H₂O₂ increased. Bovine catalase destroyed H₂O₂ and thus effectively prevented either H₂O₂-induced inhibition or acceleration of oxidation of the four substrates by potato enzyme. Horseradish peroxidase in the presence of H₂O₂ did not oxidize either monophenol, but oxidized both polyphenols. Possible association of H₂O₂, peroxidase and catalase with blackspot susceptibility is discussed.     

Blackspot of Russet Burbank potatoes and the carbon dioxide content of soil and tubers

These studies were designed to elucidate the influence of CO₂ on blackspot susceptibility of Russet Burbank potatoes. The influence of tuber CO₂ environment on blackspot was tested. Tubers from 1–4 and 6–8 inches deep in the soil were scored for blackspot and moisture samples were taken from their vicinity. Blackspot was worse in shallow tubers and in tubers from drier soil. Plowing under corn stover, covering the soil with plastic, and excessive irrigation failed to cause blackspot susceptible tubers. Diffusing CO₂ into the soil atmosphere under plastic sheets slightly increased the intensity of spot discoloration but the discoloration was atypical blackspot. Effects on blackspot by changing tuber gases was tested. Tubers whose gases had been evacuated and replaced by O₂, N₂, and CO₂ had lower blackspot scores than untreated tubers. Increasing the time tubers were soaked in water after gaseous evacuation reduced blackspot. Hydration consistently decreased tuber blackspot. In chemical studies, tubers were tested for blackspot and analyzed for CO₂ content. The relationship between tissue CO₂ and blackspot appeared to be inverse. Tuber CO₂ content was not influenced by time of day. Tuber blackspot scores immediately, 1, 3, and 7 hours after digging were the same, but tissue CO₂ content increased linearly with time after harvest.     

Cell wall thickness during growth of domestic and foreign potato cultivars

During growth of Russet Burbank tubers from less than 100 to over 250 g, thickness of parenchyma cell walls nearly doubled (1.9X) to slightly over 1 μ. Although affecting cell size at harvest maturity, different levels of N fertilizer and soil moisture had little influence on wall thickness. Wall thickness in Kennebec and Norchip increased to 0.9μ (1.7X), and in White Rose to slightly under 0.8 (1.2X). During the same growth, cell size nearly doubled in Russet Burbank. Cells of Kennebec, Norchip and White Rose averaged smaller than those of Russet Burbank at harvest maturity. Wall thickness and cell size showed similar relationships in foreign cultivars. Wall thickness in European varieties Bintje and Pimpernel was comparable to that of White Rose. Thickness in Atjimba closely approached that of Russet Burbank, and in Kamarz was similar to that of Norchip. Among South American cultivars, Doré had unusually thick walls (1.36μ), whereas those in Fruitella and Papa Bianca were between those of Norchip and White Rose. Bintje was comparable to domestic cultivars in tuber size, but all other foreign cultivars studied had medium to small tubers. Doré had unusually large cells for a small tuber. Differences in wall thickness may be due to differences in amounts of cell wall material, to differences in hydration properties of pectic components, or to combinations of both. Independently of wall thickness, the many primary pit fields in parenchyma walls may be a limiting factor in wall strength. The very thin pit membranes consist mainly of cellulosic microfibrils formed early in cell growth.     

Determination of disodium acid pyrophosphate in French-fried potatoes

An analysis procedure was developed to determine residual disodium acid pyrophosphate (SAPP) in French-fry strips after treatment. The uptake of SAPP in relation to concentrations and temperatures of the treating solution, lengths of treating time and loss of P₂O₇ from the finish-fried French fries are also considered.