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41.
Jason?J.?Z.?LiaoEmail author Timothy?L.?Schofield Philip?S.?Bennett 《Journal of Agricultural, Biological & Environmental Statistics》2005,10(2):148-157
A house standard lot is tested along with experimental samples in a variable TCID50 assay in order to monitor and control assay performance. Instead of being simply a control, it is proposed to use this lot
as a calibration standard to reduce the systematic variability in the assay caused by acknowledged sources of variability
such as the age of the cells used in the assay and interlaboratory differences. Because of this new proposal, the consistency
of the relationship between the test sample and the house standard is assessed within the acceptance range of the house standard.
A linear mixed-effects measurement error model is proposed for the data. The slope curve is then used to assess the dynamic
relationship between the sample and the house standard within the house standard range. It is shown with these analyses that
the sample and the house standard have uniformly good agreement within the house standard range. 相似文献
42.
Selma E. Guigard Warren H. Stiver Richard G. Zytner 《Water, air, and soil pollution》1996,89(3-4):277-289
Retention capacities were measured in the laboratory for n-hexane and tetrachloroethylene (PCE) in three soils at varying soil water contents. Two experimental techniques were used; 1) saturation/drainage experiments where the soil columns were saturated with the chemical and allowed to drain freely for 24 h, and 2) spill simulations where a known amount of chemical was spilled on the surface of the soil column and allowed to infiltrate for one hour. Results show that the retention capacities on a volume basis were independent of chemical type. However, the retention capacities did decrease with decreasing porosity and increasing soil water content. The decrease of retention capacity with respect to soil water content was significant, with the decreases ranging from 38% to 94%. The implication of this decrease is rapid chemical penetration into the subsurface. Retention capacities obtained from spill simulations were consistently lower than those obtained by the saturation/drainage experiments due to hysteresis. 相似文献
43.
Michele R. Derrick Jarvis L. Moyers Keith A. Yarborough Mike Warren 《Water, air, and soil pollution》1984,21(1-4):171-181
Aerosol chemistry, precipitation and visibility parameters are currently being measured at Big Bend National Park in Texas. This is part of a large-scale air resource evaluation program which the National Park Service is sponsoring in several southwestern national parks and monuments to determine the potential impact of local and distant pollutant sources on the environmental quality within these areas. Analysis of aerosol samples collected at six sites in the Southwest indicates that soil-derived components, organic materials and the acid-base ions of sulfate, nitrate, and ammonium are the major constituents of suspended airborne particulate matter in the remote areas of the arid region. Comparison of particulate matter chemistry and precipitation chemistry data at Big Bend National Park shows consistent features which indicate that the airborne alkaline soil material and NH3 largely neutralize the atmospheric acidic species of H2SO4 and HNO3. Given the similarity of the particulate matter composition and loading at the other monitoring sites, it is suggested that the trace chemical composition of precipitation will be similar in many remote regions of the Southwest. 相似文献
44.
Improved cleanup for liquid chromatographic analysis and fluorescence detection of aflatoxins M1 and M2 in fluid milk products 总被引:1,自引:0,他引:1
J Ferguson-Foos J D Warren 《Journal of the Association of Official Analytical Chemists》1984,67(6):1111-1114
A rapid method is described for extraction and cleanup of raw and processed milk for determination of aflatoxins M1 and M2 by using a C18 Sep-Pak/silica gel cleanup column combination. Aflatoxins are separated by normal phase liquid chromatography and their concentrations are determined by fluorescence detection in a silica gel-packed flow cell. Recoveries ranged from 99 to 103% with coefficients of variation less than 2% for M1 levels of 0.117-1.17 ng/mL added to raw milk. Similar recoveries were obtained for M2. The coefficient of variation for analysis of 5 subsamples of naturally contaminated milk was less than 1%. Agreement with the official method is satisfactory. Each sample requires less than 25 mL solvent and 10 min actual handling time. Sample chromatograms show no interferences in the M1-M2 elution region and no late-eluting peaks, which permits spacing injections at 13-20 min intervals. Aflatoxin levels as low as 0.03 ppb may be determined by this procedure. Extracts have also been analyzed by thin layer chromatography. 相似文献
45.
T. A. Forge M. L. Berrow J. F. Darbyshire A. Warren 《Biology and Fertility of Soils》1993,16(4):282-286
The common soil protozoan Colpoda steinii was used to study the toxicity of sulphate solutions of Ni, Cd, Cu, and Zn. The growth of C. steinii was reduced by 50% in the presence of 0.10, 0.22, 0.25, and 0.85 mg litre-1 of Ni, Cd, Cu and Zn, respectively, during 24 h of incubation at 25°C, as calculated from a regression analysis of probit-transformed data. The same growth assay was used to assess the toxicity of soil solution extracted by centrifugation from soil samples of field plots of a grass/clover ley on a sandy loam treated with sewage sludge spiked with additional Cd, Cu, Cr, Ni, Pb, or Zn at concentrations either equivalent to or twice the limits for heavy metals recommended in recent EC guidelines (Commission of European Communities directive 86/278/EEC). The toxicity of these soil solutions varied with the season of the year. None of the soil solutions extracted in winter (February 1991) inhibited the growth of C. steinii. In summer (July 1991), the growth was reduced in solutions extracted from plots that were amended with sludge plus additional Zn or Ni at twice the maxima recommended by the EC. The changes in toxicity to C. steinii of the soil solutions between February and July were positively correlated with increases in heavy metal concentrations of Zn and Ni between winter and summer. These preliminary results suggest that regular protozoan bioassays may be used to monitor the biological availability of heavy metals in soils, especially when combined with other microbial assays and with chemical analyses of soil solutions. 相似文献
46.
Coiled-coil proteins of the golgin family have been implicated in intra-Golgi transport through tethering coat protein complex I (COPI) vesicles. The p115-golgin tether is the best studied, and here we characterize the golgin-84-CASP tether. The vesicles bound by this tether were strikingly different from those bound by the p115-golgin tether in that they lacked members of the p24 family of putative cargo receptors and contained enzymes instead of anterograde cargo. Microinjected golgin-84 or CASP also inhibited Golgi-enzyme transport to the endoplasmic reticulum, further implicating this tether in retrograde transport. These and other golgins may modulate the flow patterns within the Golgi stack. 相似文献
47.
Formation of virus-like particles by bone cells in mice with a high incidence of spontaneous leukemia 总被引:2,自引:0,他引:2
B H Schofield C P Barrett S B Doty F H Figge R A Robinson 《Science (New York, N.Y.)》1970,168(931):588-589
Bone samples from potentially leukemic and leukemic mice revealed numerous 90-to 110-nanometer particles morphologically identical to murine leukemia virus. Particles were observed budding from plasma membranes of osteocytes and osteoblasts but were most numerous in osteocyte lacunae. Particles were not observed in bone samples from mice which rarely develop leukemia. 相似文献
48.
Taylor FW Diner DJ Elson LS McCleese DJ Martonchik JV Delderfield J Bradley SP Schofield JT Gille JC Coffey MT 《Science (New York, N.Y.)》1979,205(4401):65-67
Further results from the Venus orbiter radiometric temperature experiment (VORTEX) on the Pioneer orbiter are presented. These are used to characterize the three-dimensional temperature field, the cloud structure, and the dynamics of the 60-to 130-kilometer altitude region of the Venus atmosphere. One of the new discoveries is a "dipole" structure at high latitudes, with two hot spots rotating around the pole, surrounded by banks of cold cloud. 相似文献
49.
Pham KN Puertas AM Bergenholtz J Egelhaaf SU Moussaïd A Pusey PN Schofield AB Cates ME Fuchs M Poon WC 《Science (New York, N.Y.)》2002,296(5565):104-106
Experiments, theory, and simulation were used to study glass formation in a simple model system composed of hard spheres with short-range attraction ("sticky hard spheres"). The experiments, using well-characterized colloids, revealed a reentrant glass transition line. Mode-coupling theory calculations and molecular dynamics simulations suggest that the reentrance is due to the existence of two qualitatively different glassy states: one dominated by repulsion (with structural arrest due to caging) and the other by attraction (with structural arrest due to bonding). This picture is consistent with a study of the particle dynamics in the colloid using dynamic light scattering. 相似文献
50.
Similarity of synthetic peptide from human tumor to parathyroid hormone in vivo and in vitro 总被引:18,自引:0,他引:18
N Horiuchi M P Caulfield J E Fisher M E Goldman R L McKee J E Reagan J J Levy R F Nutt S B Rodan T L Schofield 《Science (New York, N.Y.)》1987,238(4833):1566-1568
One mechanism considered responsible for the hypercalcemia that frequently accompanies malignancy is secretion by the tumor of a circulating factor that alters calcium metabolism. The structure of a tumor-secreted peptide was recently determined and found to be partially homologous to parathyroid hormone (PTH). The amino-terminal 1-34 region of the factor was synthesized and evaluated biologically. In vivo it produced hypercalcemia, acted on bone and kidney, and stimulated 1,25-dihydroxy-vitamin D3 formation. In vitro it interacted with PTH receptors and, in some systems, was more potent than PTH. These studies support a long-standing hypothesis regarding pathogenesis of malignancy-associated hypercalcemia. 相似文献