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排序方式: 共有1371条查询结果,搜索用时 62 毫秒
131.
132.
Walker AS Gautier AL Confais J Martinho D Viaud M Le P Cheur P Dupont J Fournier E 《Phytopathology》2011,101(12):1433-1445
Botrytis cinerea is a major crop pathogen infesting >220 hosts worldwide. A cryptic species has been identified in some French populations but the new species, B. pseudocinerea, has not been fully delimited and established. The aim of this study was to distinguish between the two species, using phylogenetic, biological, morphological, and ecological criteria. Multiple gene genealogies confirmed that the two species belonged to different, well-supported phylogenetic clades. None of the morphological criteria tested (spore size, germination rate, or mycelial growth) was able to discriminate between these two species. Sexual crosses between individuals from the same species and different species were carried out. Only crosses between individuals from the same species were successful. Moreover, population genetics analysis revealed a high level of diversity within each species and a lack of gene flow between them. Finally, a population survey over time showed that B. cinerea was the predominant species but that B. pseudocinerea was more abundant in spring, on floral debris. This observation could not be explained by temperature adaptation in tests carried out in vitro or by aggressiveness on tomato or bean leaves. This study clearly establishes that B. cinerea and B. pseudocinerea constitute a complex of two cryptic species living in sympatry on several hosts, including grapevine and blackberry. We propose several biological or molecular tools for unambiguous differentiation between the two species. B. pseudocinerea probably makes a negligible contribution to gray mold epidemics on grapevine. This new species has been deposited in the MycoBank international database. 相似文献
133.
Rates of degradation of seven organophosphate nematicides and insecticides were examined in two soils known to show accelerated biodegradation of fenamiphos and one soil known to show accelerated biodegradation of chlorpyrifos. The results indicated that several organophosphate insecticides and one nematicide were susceptible to cross-enhanced degradation in the soil showing accelerated biodegradation of chlorpyrifos. No cross-enhancement was observed in the two soils showing accelerated degradation of fenamiphos. Fumigation resulted in the complete inhibition of pesticide degradation in all soils. The data suggested that the cross-enhancement of selected pesticides in chlorpyrifos-degrading soil was dependent on the structural similarity of the compounds. Mechanisms of degradation of pesticide in soil support this hypothesis, where structurally similar compounds (diazinon, parathion, coumaphos and isazofos) were hydrolysed by microbial activity in chlorpyrifos-degrading soil but the degradation products were accumulated. Enhanced degradation of chlorpyrifos and fenamiphos was found to be stable in the laboratory condition for a period of one year. 相似文献
134.
Walker MK Farkas DF Anderson SR Meunier-Goddik L 《Journal of agricultural and food chemistry》2004,52(26):8230-8235
High-pressure processing (HPP) was utilized to induce unfolding of beta-lactoglobulin (beta-LG). beta-Lactoglobulin solutions at concentrations of 0.5 mg/mL, in pH 7.5 phosphate buffer, were pressure treated at 510 MPa for 10 min at either 8 or 24 degrees C. The secondary structure, as determined by circular dichroism (CD), of beta-LG processed at 8 degrees C appeared to be unchanged, whereas beta-LG processed at 24 degrees C lost alpha-helix structure. Tertiary structures for beta-LG, as determined by near-UV CD, intrinsic protein fluorescence spectroscopy, hydrophobic fluorescent probe binding, and thiol group reactivity, were changed following processing at either temperature. The largest changes to tertiary structure were observed for the samples processed at 24 degrees C. Model solutions containing the pressure-treated beta-LG showed significant decreases in surface tension at liquid-air interfaces with values of 54.00 and 51.69 mN/m for the samples treated at 24 and 8 degrees C, respectively. In comparison, the surface tension for model solutions containing the untreated control was 60.60 mN/m. Changes in protein structure during frozen and freeze-dried storage were also monitored, and some renaturation was observed for both storage conditions. Significantly, the sample pressure-treated at 8 degrees C continued to display the lowest surface tension. 相似文献
135.
Epiphyte mats (contiguous pieces of live and dead epiphytes perched upon branches of trees) are a conspicuous component of tropical cloud forests and harbor diverse meso- and microarthropod communities. We investigated differences in arthropod assemblage structure between the vegetative (green) and humic (brown) portions of epiphyte mats in a lower montane forest in Monteverde, Costa Rica. Because of qualitative differences between the two substrates, we hypothesized that they would support different arthropod communities and that variation in community parameters would be linked to the quantity of brown material present in a mat sample. The green fraction contained twice as many individuals and species per gram dry mass than the brown fraction. Morphospecies composition was very similar between green and brown portions, but the relative abundance of several taxa differed significantly between the substrates. Contrary to our prediction, total arthropod abundance and richness in a sample were not correlated with the proportion of brown material present. In laboratory trials, the most common morphospecies of oribatid mite in this system showed a preference for brown substrates. 相似文献
136.
Yucheng Feng Donald M. Stoeckel Edzard van Santen Robert H. Walker 《Biology and Fertility of Soils》2002,36(6):456-460
The sensitivity of creeping bentgrass (Agrostis palustris Huds.) to the extreme heat found in the southeastern United States has led to the development of new greens-management methods. The purpose of this study was to examine the effects of subsurface aeration and growth regulator applications on soil microbial communities and mycorrhizal colonization rates in a creeping bentgrass putting green. Two cultivars (Crenshaw and Penncross), a growth regulator (trinexapac-ethyl), and subsurface aeration were evaluated in cool and warm seasons. Total bacterial counts were higher in whole (unsieved) soils than in sieved soils, indicating a richer rhizosphere soil environment. Mycorrhizal infection rates were higher in trinexapac-ethyl (TE) treated plants. High levels of hyphal colonization and relatively low arbuscule and vesicle occurrence were observed. Principal components analysis of whole-soil fatty acid methyl ester (FAME) profiles indicated that warm-season microbial populations in whole and sieved soils had similar constituents, but the populations differed in the cool season. FAME profiles did not indicate that subsurface aeration and TE application affected soil microbial community structure. This is the first reported study investigating the influences of subsurface aeration and TE application on soil microorganisms in a turfgrass putting green soil. 相似文献
137.
The attachment of Escherichia coli and Streptococcus bovis of bovine faecal origin to soil clay particles has been examined using scanning electron microscopy. Both organisms produce extracellular polymers through which the cells form bridges to clay surfaces. Differences in the extent of cell-to-particle bridging between E. coli-and S. bovis-soil complexes are explained in terms of the charge characteristics of the cells and the properties of the exopolymers. 相似文献
138.
The fate of phosphorus during pedogenesis 总被引:2,自引:0,他引:2
The fate of phosphorus (P) during pedogenesis is considered with particular emphasis on vertical distribution within the profile. Information is based on P fractionation studies involving a range of soil sequences in New Zealand. Forms of P in soil parent materials are discussed in relation to soil P transformations. Soil chronosequences, developed in contrasting environments, provide useful information on the pedogenetic pathways of soil P. Although less well understood, soil toposequences can be used to elucidate the effects of slope and drainage on soil-forming processes which involve P. 相似文献
139.
Robert C. Ferrier Hamish A. Anderson John D. Miller T. A. Bruce Walker Nils Christophersen 《Water, air, and soil pollution》1992,62(3-4):281-302
A detailed study of hydrochemical processes associated with vegetation and soils was undertaken at a pristine site in mid-Norway. Inputs to the site during snow-free periods showed a marked seasonality with excess sulphate concentrations in rainfall only during the summer months. However, this represented a small fraction of total annual loading and there was a dominant influence of sea salt deposition. Site vegetation modified the chemistry of water reaching the soil surface, and this also showed a marked seasonal response. The pristine nature of this site results in the soils retaining acid-soluble components which may have been eluted from impacted sites. In the soils, the extractable aluminium remains largely (70%) in the organic bound forms. The contribution of different sources of water in affecting streamwater output quality at this site are discussed. 相似文献
140.
Solberg S. Lazaridis M. Walker S.-E. Knudsen S. Semb A. 《Water, air, and soil pollution》2003,148(1-4):289-321
A photochemical puff-trajectory model (Fotoplume) has been applied to simulate emissions, atmospheric transport and chemical transformations of pollutants from offshore oil and gas production in the North Sea. The above model was used in conjunction with the European Monitoring and Evaluation Programme (EMEP) regional Lagrangian oxidant model. The Fotoplume and EMEP models were used to evaluate the effects of the atmospheric emissions from the oil and gas exploration activity in the Norwegian sector of the North Sea. Deposition of nitrogen and formation of boundary level ozone in Southern Norway due to North Sea emissions of nitrogen oxides (NOx), carbon monoxide (CO) and volatile organic compounds (VOC) have been studied. The petroleum activity in the North Sea is calculated to contribute approximately 20% of the nitrogen deposition in the coastal areas of Norway in 1992. In addition, the models were used to estimate the AOT40 ozone exposure levels. The results indicate that emissions from British and Norwegian oil and gas exploitation sector separately contribute to less than 5% each of the AOT40 values for coniferous forests and meadows. Comparison of model calculations with experimental measurements is quite satisfactory and the models show realistic results for both the nitrogen deposition and AOT40 values. 相似文献