Specific hybridization real‐time PCR probes for Phytophthora ramorum detection and diagnosis

Sudden Oak Death, caused by Phytophthora ramorum, poses a serious threat to native American oaks, and is also present in Europe where it has been isolated from numerous European ornamental plant nurseries. Its proven aggressiveness against plants in the Fagaceae and Ericaceae and the damage it has caused in North America have lead to it being assigned quarantine status. The timely and accurate detection of P. ramorum is a critical aid in the study of the epidemiology and biology of this pathogen. As a regulated organism, the availability of a sensitive and reliable assay is essential when attempting to achieve early detection of the pathogen. In this work, new specific hybridization probes for a real‐time PCR amplification method were found to be rapid, robust and labour‐saving, and proved suitable for routine use in a molecular diagnostic laboratory.     

Proteomic analysis of membrane preparations from developing Pinus radiata compression wood

For coniferous gymnosperms, few data exist as to the contribution of the membrane-associated proteome to cell wall and wood formation. In this study, we begin to address this knowledge deficiency by examining the proteomic profile of Golgi-enriched membrane preparations derived from developing Pinus radiata compression wood. These membrane preparations were generated by a combination of discontinuous sucrose gradient centrifugation and Triton X-114-based phase separation. Fractionation by phase separation removed contaminating proteins associated with the cytoskeleton and enabled the discrimination between soluble and membrane-bound/integral proteins. The proteomic analysis of the resulting aqueous and detergent phases using high-performance liquid chromatography-tandem mass spectrometry resulted in the identification of 175 proteins. The majority of the identified proteins were membrane bound/integral and originated from cellular components such as the nucleus, plastids, endoplasmic reticulum, plasma membrane and Golgi vesicles. On the basis of bioinformatic analysis, many of the identified proteins were predicted to be involved either in the regulation of wood formation or in cell wall biosynthesis, which indicated that the proteomic analysis of non-cytosolic proteins in developing xylem is a useful strategy to investigate the molecular aspects of wood formation in pine.     

Intestinal antiinflammatory activity of a lyophilized infusion of Turnera ulmifolia in TNBS rat colitis

Turnera ulmifolia is a plant popularly known in Brazil and South America as chanana. Some species of Turnera are widely used in folk medicine for different types of inflammatory diseases. In this study, the preventive intestinal antiinflammatory activity of a lyophilized infusion obtained from the aerial parts of T. ulmifolia was tested in the trinitrobenzenesulphonic acid (TNBS) model of rat colitis. The results obtained revealed that pretreatment to colitic rats with the extract, at 250 and 500 mg/kg, significantly attenuated the colonic damage induced by TNBS. This beneficial effect was associated with an improvement in the colonic oxidative status, since the infusion prevented the glutathione depletion that occurred as a consequence of the colonic inflammation. On the other hand, this antioxidant activity was confirmed in in vitro studies. In conclusion, the preventive effect exerted by the lyophilized infusion of T. ulmifolia in the TNBS model of rat colitis is probably related to its antioxidant properties, due to its flavonoids content.     

Vertikaler Wasser- und Nitrattransport in tiefere Bodenschichten süddeutscher Ackerstandorte

Vertical water and nitrate movement into deeper soil layers on fields located in the south of Germany In Southern Bavaria, selected fields from deep loess and sandy sediments were sampled to a depth of maximum 10 m every 4 months, in order to determine the basic processes of water and nitrate movement in the course of the year. The downward movement of water and nitrate followed the principle of piston flow only in the intermediate vadose zone of loess soils with a leaching distance amounting 0.8 m per year. On the other hand, an accelerated as well as a delayed transport was observed in the zone of evapotranspiration of loess soils and in the whole profile of sandy soils, not being in accordance with the simple simulation model FLOTRA based on the classic theories of transport (Darcy flow, convection-dispersion-equation). Preferential flow led to the leaching of nitrate from the top soil to the lower boundary of the evapotranspiration zone of loess soils in 2 m depth, in winter and spring. In sandy soils nitrate was leached to the groundwater table in 4–6 m depth after extensive rainfall.     

Dynamics of Atmospheric Aerosol Number Size Distributions in the Eastern Mediterranean During the ��SUB-AERO�� Project

Measurements of number size distributions of submicron aerosols have been performed at the Eastern part of Mediterranean as part of an extensive measurement campaign to study photo-oxidants and aerosols (SUB-AERO Project). The measurements were made at the Finokalia station on the island of Crete (Greece) and onboard the research vessel ??Aegaeon??. Two campaigns were performed during July 2000 and January 2001 using two scanning mobility particle sizers. The particle distributions measured in the range between 7.8 < d _p < 327 nm during the summer measurements and between 7.5 < d _p < 316 nm during the winter measurements, where d _p is the mobility particle diameter. The concentration of ultrafine particles (7.5 < d _p < 30 nm) was higher during the winter period and varied mainly between 5 × 10¹ and 2 × 10³?cm^?3 with concentration peak values for this mode exceeding 1 × 10⁴?cm^?3. During the summer campaign, an average number concentration of 1 × 10²?cm^?3 at Finokalia and about 5 × 10¹?cm^?3 aboard the ??Aegaeon?? vessel was measured. An average concentration of 1 × 10³?cm^?3 was measured for the particles in the size range between 30 and 100 nm, whereas in the size range 100?C300 nm, the measured concentration ranged between 1 × 10² and 5 × 10³?cm^?3. Diurnal patterns in number concentrations were observed in connection with the transport of air masses and local sources. During the winter period, three nucleation events were observed in connection with the appearance of a particle mode at 20 nm.     

Centimeter-scale variability in the chemistry of convective rains

A standard National Atmospheric Deposition Program (NADP) rain collection bucket was sectioned with 220 test tubes of 1.5 cm diameter. Eleven rains from thunderstorms were collected. Analyses were made for Ca, Na and K concentrations in the test tube liquids. The relative standard deviation (RSD), after correction for the analytical RSD, was 0 to 15% for Ca, 0 to 24% for Na, and 0 to 17% for K. Analytical RSD for Ca, Na and K determined from standards were, respectively, 5.4, 6.6 and 12%. Calculations indicate that the difference of 2 to 3 giant nuclei per test tube of calcite (Ca), sea salt (Na) and clay (K) would account for these different RSDs. Concentration ranges in the rains varied from 0.14 to 2.95 mg L^?1 for Ca, 0.024 to 0.23 mg L^?1 for Na, and 0.014 to 0.064 mg L^?1 for K. The area between the test tubes which was 40% of the total collector area provided a bottom sample which on average agreed within 1.3% of the average value of the test tubes.     

A Fluted Point from the Uptar Site, Northeastern Siberia

Lanceolate bifacial points, including one fluted specimen, have been collected from beneath an early Holocene tephra at the Uptar site, northeastern Siberia. Thus, the technology associated with the well-known Paleoindian tradition was not confined to the Americas. The Uptar collection does not compare readily with other Beringian complexes and demonstrates that there is greater diversity in the archaeological record of northeastern Siberia than traditional colonization models imply.     

Fission track ages and ages of deposition of deep-sea microtektites

The Australasian and Ivory Coast deep-sea microtektites have fission track ages of 0.71 and 1.09 million years, respectively. These ages are in good agreement with the ages of deposition of the microtektites determined from paleomagnetic data. Both the fission track ages and ages of deposition of the microtektites agree with the potassium/ argon and fission track ages of tektites from the respective tektite strewn fields.     

Artificial Insemination of Gilts with 1.5 Billion Sperms Stored in Different Periods Associated with Different Pre-ovulatory Intervals

This study evaluated the reproductive performance of gilts inseminated at three intervals before ovulation (0-12, 13-23, 24-30 h) with sperm doses (SD) stored for 0-48 and 96-120 h. A total of 218 PIC Camborough 22 gilts were inseminated once with SD of 1.5 x 10(9) sperms. Pregnant gilts (n = 166) were slaughtered 30.8 +/- 3.7 days after artificial insemination. The number of corpora lutea (CL) and total embryos (TE) was counted. Pregnancy rates (PR) were analysed by chi-square test. TE and embryonic survival (ES), obtained as the ratio between viable embryos and CL, were analysed by GLM procedure (SAS) and mean values were compared by Tukey's test. Pregnancy rate was similar among artificial insemination-ovulation (AIOV) intervals when semen was stored for 0-48 h. However, the lowest PR was observed in the 24-30 h AIOV interval with storage time (ST) of 96-120 h (p < 0.05). There was a significant effect of the interaction between ST and AIOV (p < 0.05) on TE and ES variables. Total embryos and ES did not differ (p > 0.05) among AIOV intervals in ST of 0-48 h. However, gilts inseminated at 24-30 h AIOV interval with ST of 96-120 h showed a reduction of 6.7 embryos (p < 0.05) compared with gilts in the same interval inseminated with semen stored for 0-48 h. ES for the 24-30 h AIOV interval and ST of 96-120 h was lower than that observed in the other groups (p < 0.05).     

The impact of harvester ants on decomposition, N mineralization, litter quality, and the availability of N to plants in the Mojave Desert

In arid areas of North America, nests of the seed-harvesting ant Pogonomyrmex rugosus tend to be elevated in mineral nitrogen and other soil nutrients relative to other microhabitats. We investigated the roles of decomposition, N mineralization, and plant nutrient uptake in maintaining high standing stocks of nutrients in P. rugosus ant nests. Decomposition rates of standard cellulose substrates placed on the surface of ant nests and other desert microhabitats suggest that conditions found in ant nests and bare areas are conducive to higher rates of decomposition than conditions under shrubs. In laboratory incubations of moist soil, net N mineralization rates were significantly higher in soil from ant nests than from bare areas and under two of three plant species. Net N mineralization rates measured in situ were much lower than those measured in laboratory incubations, but ant nest soil still exhibited higher rates at one of two sites. Litter collected from ant mounds, composed chiefly of seed chaff, was similar in N content to litter collected from underneath the dominant plant species, but had a significantly higher mean δ¹⁵N. Using this distinctive isotope signature as a tracer, we found no evidence that large perennial shrubs tap ant nests as a source of N. An invasive, annual grass species was significantly enriched in ¹⁵N, had higher leaf %N, and produced more seeds when growing on the mound than when growing several meters away; however P. rugosus nest surfaces are typically free of such annuals. We conclude that both high rates of nutrient cycling relative to other Mojave Desert microhabitats and low N utilization by the surrounding vegetation contribute to high standing stocks of mineral N in P. rugosus nests.