长江近口段鳗苗捕捞量的时间格局及其与生态因子的GAM模型分析

为研究长江口鳗苗捕捞量与生态因子的相互关系,于2012年汛期对长江靖江段鳗苗的捕捞量进行了监测,采用广义可加模型(GAM)对日捕捞量与水温、潮差、气压、浑浊度等生态因子之间的相关性作了分析。结果显示,靖江段鳗苗汛期为1月下旬—4月上旬,单船总捕捞量为221~443尾,平均(344.8±83.4)尾。1月均值仅0.4尾/d,且空网率高达90.9%;4月为旺汛期,均值10.4尾/d,空网率仅为10.0%。GAM模型显示,潮汐周期—月份交互项、水温和潮差对鳗苗日捕捞量的影响显著,而气压、浊度和月相周期对鳗苗日捕捞量的影响不显著。潮汐周期—月份交互项、水温和潮差对鳗苗日捕捞量的偏差解释率分别为42.4%、19.1%和13.1%,均呈现正相关关系。统计也显示,日捕捞量表现出上、下弦月较低、新月或满月前后较高的半月周期波动。鳗苗捕获的最低水温为6.3℃,而10~15℃为适宜捕捞水温。高潮期和低潮期分别占总捕捞量的76.8%和23.2%。研究表明,长江口鳗苗在借助潮汐流而快速溯河的过程中,部分在口门水域即被捕获,部分滞留在了长江河口段,而影响鳗苗溯河的重要生态因子是潮汐和水温。     

饲料氧化鱼油引起草鱼肠道黏膜结构屏障损伤

为了研究饲料氧化鱼油对草鱼肠道屏障结构和肠道通透性的影响。(实验目的)以豆油、鱼油、氧化鱼油作为饲料脂肪源,分别设计鱼油组(6F)、豆油组(6S)、2%氧化鱼油(2OF)、4%氧化鱼油(4OF)及6%氧化鱼油(6OF)5组等氮、等能半纯化饲料,在池塘网箱养殖草鱼(平均体重74.8士1.2)72d。采用实时荧光定量PCR(qRT-PCR)的方法,测定了草鱼肠道紧密连接蛋白中闭锁蛋白Occludin,闭合蛋白Claudin3、Claudin15a和胞浆蛋白ZO-1、ZO-2、ZO-3的基因表达活性,并结合肠黏膜细胞间紧密连接结构透射电镜观察结果、肠道通透性标志性指标测定结果综合分析。(实验方法)结果显示：在添加氧化鱼油后,(1)血清二胺氧化酶活性、内毒素和D-乳酸含量都出现显著增加(P<0.05);(2)组成肠黏膜细胞间紧密连接结构的闭合蛋白Claudin-3、Claudin-15a和胞浆蛋白ZO-1、ZO-2、ZO-3基因表达活性显著下调(P<0.05),而闭锁蛋白Occludin基因表达活性出现不同程度的下调,但差异不显著(P>0.05);(3)透射电镜观察结果显示肠黏膜细胞间紧密连接结构出现缝隙;⑷饲料中的AV值、POV值、MDA含量与闭合蛋白Claudin-3、Claudin-15a,胞浆蛋白ZO-1、ZO-2、ZO-3和闭锁蛋白Occludin基因表达活性均显示负相关关系的变化趋势,其中饲料POV值、MDA含量与胞浆蛋白ZO-1基因表达活性呈极显著负相关关系(P<0.05)。(实验结果)结果表明,在饲料中添加氧化鱼油后,氧化鱼油中的过氧化物、丙二醛等油脂氧化产物导致组成肠道黏膜细胞间紧密连接结构的蛋白基因表达活性显著下调、紧密连接物理性结构显著损伤,致使肠黏膜屏障结构的完整性被破坏、肠黏膜通透性显著增加。(实验结论)     

长江上游原生动物的群落生态学研究

于2003～2007年采集了长江上游通天河至三峡水库的原生动物样品,对长江上游地区原生动物的分布情况作了较全面的调查.该调查共设93个采样站点,其中通天河-重庆在长江干流设38个采样站,支流设22个采样站,三峡库区设33个采样点,每年丰水期和平水期各采集1次.调查结果表明,长江上游共检出309种原生动物,隶属于60科106属,其中通天河至金沙江段检出140种,水富-重庆江段检出90种,三峡水库检出245种.金沙江段优势种为砂壳虫、匣壳虫、表壳虫和圆壳虫;水富-重庆段优势种为砂壳虫、匣壳虫及巢居法帽虫;三峡库区种类较为丰富,除了砂壳虫科和表壳科依然占据优势外,简壳虫、变形虫、领鞭虫、吸管虫和钟虫也逐渐增多,成为一些区域的优势种.三峡水库检出的原生动物种类多于上游河流段,其中有125种只在三峡库区检出.与1990年三峡地区原生动物资料相比.库区的群落结构发生了明显变化.随着水库水文情势的变化,原生动物种类组成也由以有壳肉足虫为主的河流型逐渐转变为以纤毛虫占优势的静水型种类.     

优质抗寒、抗病酿造葡萄新品种‘北红’

 ‘北红’葡萄新品种由‘玫瑰香’与‘山葡萄’杂交育成。浆果在北京地区9月底成熟。果粒圆形,蓝黑色,果粒质量1.57g,果穗质量160.0 g,浆果可溶性固形物含量23.8 %～ 27.0 %,含酸量0.89～1.26 %。早果性及丰产性强。抗寒、抗病能力特强。酿成的酒深宝石红色,酒体平衡,酒质上等。     

春秋大棚茄子专用新品种‘茄杂6号’

 ‘茄杂6号’是由自交系‘134’与‘圆杂-黑扁-1-1-M’配制而成的春秋大棚茄子专用品种。该品种耐弱光,易坐果,着色好,抗性强。株形紧凑,门茄节位8节左右。果实扁圆形,果色黑亮,果肉浅绿,单果质量850~950g,商品性好。产量一般为90~105 t·hm^-2。     

Identification of serum differential proteins in colorectal adenoma and early malignantly transformed adenoma by proteomics

AIM: To investigate the associated proteins and sensitive biomarkers for early diagnosis of colorectal adenocarcinoma by comparing the results of differential proteomic analysis between colorectal adenoma and early malignantly transformed adenoma. METHODS: Two-dimensional gel electrophoresis was used to define patterns of protein expressions of colorectal adenoma and early malignantly transformed adenoma. Proteins expressed differentially among groups were detected, cut out and analyzed by MALDI-TOF/TOF mass spectrometry. RESULTS: Two-dimensional protein maps of colorectal adenoma and early malignantly transformed adenoma were analyzed with gel-analysis software, an average of 1 672 spots in adenoma, 1 732 in early malignantly transformed adenoma were observed. 28 spots of a 1.5-fold change were found, including 15 proteins down-regulated and 13 up-regulated in early malignantly transformed adenoma, in which 23 proteins were identified by mass spectrometry, the rate of identification was 82.14%. 13 differential proteins were attained, 8 were up-regulated and 5 were down-regulated, which was classified to 6 categories, including protease inhibitor, complement, immunoglobulin, keratoproteins, signal transduction protein and function-unknown proteins. CONCLUSION: The changes of serum proteins in early malignantly transformed adenoma from adenoma can be identified by proteomic technology. Proteins detected in the study may provide new biomarkers correlated with biological behavior of colorectal adenocarcinoma.     

银杏叶对辣椒植株发酵液自毒作用的化感调控

采用1/2霍格兰（Hoagland）营养液水培法,研究辣椒植株发酵液的自毒作用及银杏叶发酵液和 再腐解液对其的调控作用。结果表明,辣椒植株发酵液使辣椒受到自毒作用,根系活力下降,相对电导 率增大。银杏叶发酵液加重了辣椒植株发酵液的自毒作用,并且与其浓度成正相关。银杏叶再腐解液缓 解了辣椒的自毒作用,缓解效果与再腐解液的浓度有关。     

洋葱细胞质雄性不育系选育研究进展

 介绍了国内外洋葱细胞质雄性不育（CMS）系的选育及利用概况;对洋葱细胞质雄性不育机理和相关分子标记进行了综述;在此基础上对洋葱雄性不育的研究提出了几点建议。     

Jagged1 knocking down in endothelial cells accelerates PDGF induced proliferation and migration of vascular smooth muscle cells in rats

AIM: To investigate the effect of Jagged1 expression in endothelial cells (EC) on platelet derived growth factor (PDGF) induced proliferation and migration of vascular smooth muscle cells (VSMC) in rat.METHODS: Rat aorta EC was inoculated in the lower chamber and VSMC were in the upper chamber of the cell coculture system. Three groups were divided: control, sicontrol and siJagged1. The EC Jagged1 protein expression was assayed by Western blotting to evaluate small RNA interfering (RNAi) efficiency. After the cells were cocultured with PDGF for 24 h, the proliferation and migration of VSMC were respectively evaluated by ［3H］-TdR incorporation and migrating cells counting. Protein expression of α-SM-actin in VSMC was assayed by Western blotting. RESULTS: The Jagged1 protein expression in EC was significantly lower in siJagged1 group than that in control group (0.26±0.02 vs 0.67±0.02, P<0.05), and no statistic significance was observed between control and sicontrol groups. The VSMC ［3H］-TdR incorporation and migration were higher in PDGF +siJagged1 group than those in PDGF group {［3H］-TdR incorporation (23 074±2 702) counts·min-1·well-1 vs (16 442±1 803)counts·min-1·well-1, n=5, P<0.05; migration (27±4) cells/field vs (15±3)cells/field, n=5, P<0.05}. The α-SM-actin protein in VSMC was lower in PDGF + siJagged1 group than that in PDGF group (0.25±0.06 vs 0.49±0.04, n=3, P<0.05).CONCLUSION: Jagged1 knock down in rat EC accelerates PDGF induced proliferation and migration of VSMC. These results suggest that Jagged1 expression in EC plays an important role in maintaining VSMC contract phenotype and inhibiting VSMC overgrowth after arterial injury.