The effect of physical form of a silage on its voluntary consumption and digestibility by sheep

Six castrated male sheep were used in a two-period crossover experiment to investigate the effect of mincing on the voluntary intake and digestibility of a silage. The silage was a high-quality material, containing 68 % digestihle organic matter in the dry matter, prepared from S24 perennial ryegrass cut with a precision chop forage harvester and ensiled with the addition of 2.3 1 t^?1 formic acid as preser vative. The median size of particles in the unprocessed silage was 10–20 mm and in the minced silage 2–3 mm. The intakes of dry matter, organic matter and nitrogen for the minced silage were greater than for the unminced material, and for dry matter and organic matter intakes the differences were signi ficant (P<0.01). Mincing was associated, however, with a depression in the digestibility of all three constituents which offset the increase in intake. The intakes of digestible organic matter were 817 g d^-1 for the minced silage and 729 g d^?1 for the unminced material (P>0.05). Corresponding figures for digestible nitrogen intakes were 208 gd^?1and23.0 gd^?1 (P>005). It is concluded that with high-quality silage diets there is a physical component in the mechanism for the control of voluntary food intake. It is suggested that for sheep, depressions in the digestibility of dietary constituents are unlikely to occur until silages are chopped to a median particle size of 5–10 mm.     

A comparison of a rising-plate meter and an electronic capacitance meter for estimating the yield of pastures grazed by dairy cows

The performances of a rising-plate meter and a single-probe electronic capacitance meter were compared for estimating the herbage mass of irrigated pastures both before and after grazing by dairy cows. Mean CV for the rising plate meter and the single-probe meter were 12.7% and 13.3% respectively when herbage mass was measured before grazing, and 21.8% and 15.4% respectively immediately after grazing. These coefficients of variation indicate that while the performance of the two meters was similar when herbage mass was measured before grazing, the single-probe meter was a more precise instrument for estimating herbage mass after grazing.
Trampling of herbage prior to measurement had a major effect on the regressions of both meters; the regression intercept was increased by 2800 kg dry matter (DM) ha⁻¹ for the rising-plate meter and by 2580 kg DM ha⁻¹ for the single-probe meter. The slopes of the graphs, however, remained unchanged.
It was concluded that while the single-probe meter performed better than the rising-plate meter for estimating the mass of post-grazing herbage when trampling was negligible, the large effect that trampling had on the post-grazing calibration regressions precluded both instruments from general use in dairy cattle research. This indicates that another technique is required in circumstances in which pastures are heavily trampled.     

Radiation dose rate and mutation frequency

New data have clearly confirmed the earlier finding that specific locus mutation rates obtained with chronic gamma irradiation of spermatogonia are lower than those obtained with acute x-rays. Since this result is in contrast to classical findings for Drosophila spermatozoa, and apparently contradicts one of the basic tenets of radiation genetics, it was important to determine what factors were responsible for it. Experiments undertaken for this purpose reveal the following: (i) the lower mutation frequency is due mainly to difference in dose rate of radiation, rather than quality; (ii) a dose-rate effect is not obtained in experiments with mouse spermatozoa, confirming classical findings for spermatozoa, and indicating that the explanation for intensity dependence in spermatogonia resides in some characteristic of gametogenic stage; and (iii) a dose-rate effect is found not only in spermatogonia but also in oocytes, where cell selection is improbable, indicating that the radiation intensity effect is on the mutation process itself. A threshold response for all mutations in spermatogonia and oocytes is not a necessary consequence of the findings. Plausible hypotheses consistent with the present results can lead to other predictions. From a practical point of view, the results indicate that the genetic hazards, at least under some radiation conditions, may not be as great as those estimated from the mutation rates obtained with acute irradiation. However, it should not be forgotten that even the lower mutation rates obtained with the present intensity levels are still appreciable (16).     

Assessment of organoleptic postmortem inspection techniques for bovine offal

Objective To evaluate organoleptic postmortem inspection techniques for bovine livers and kidneys.
Study design At Australian export abattoirs, bovine liver and kidneys are assessed and graded by qualified meat inspectors during normal operations. Over a 12-month period at a large abattoir in eastern Australia during 1997 and 1998, a sample of these organs was reassessed independently using a range of pathological and microbiological methods. Agreement between routine inspection and independent assessment was evaluated using methods of inter-rater agreement.
Results A total of 944 livers and 1374 kidneys were included in the study. All of these organs had been classified during routine inspection. The authors examined 363 livers and 329 kidneys both grossly and histologically, including 36 livers and 14 kidneys that were also subjected to microbiological examination. All other organs were only examined grossly. There was only a moderate level of agreement between the routine and independent assessment methods. For livers, the percentage agreement was 80.2%, McNemar's test of symmetry 55.2 (3 degrees of freedom, P < 0.001) and kappa 0.63. For kidneys, the percentage agreement was 67.8%, McNemar's test of symmetry 9.9 (1df, P = 0.002) and kappa 0.35.
Conclusions The results reinforce concerns from a number of authors about organoleptic postmortem inspection. Risk assessment methodologies offer the opportunity to modify inspection techniques in a manner that is most relevant to current public health concerns.