Evaluation of an Interfragmentary Compression System for the Repair of Equine Femoral Capital Physeal Fractures

Femoral neck and proximal epiphyseal lengths were measured in 37 femurs from 19 cadaver foals that were 1 day to 12 months old to determine the applicability of a human interfragmentary compression system to equine femoral capital physeal fractures. Because components of the implant system are available only in fixed sizes, its use was possible in foals older than 5 weeks of age, but not in younger foals. The 135 degree angle plate conformed best to the equine femur. Femoral capital physeal fractures were created surgically and repaired with the implant system in three foals. Fracture stability was evident clinically and radiographically in all three foals until euthanasia at month 3. At necropsy, the treated femurs were 4, 8, and 27 mm shorter than their mates. Epiphyseal viability was verified in all three foals by tetracycline deposition and new appositional bone growth comparable with that in the contralateral control epiphyses. The treated capital physis was open but reduced in thickness in one foal, disorganized in one foal, and closed in one foal. Fixation by compression with the implant system resulted in stability sufficient for fracture healing and maintenance of epiphyseal viability, although it was associated with reduced longitudinal femoral growth.     

Surgical Repair of an Intrathoracic Esophageal Pulsion Diverticulum in a Horse

An intrathoracic esophageal pulsion diverticulum causing repeated episodes of esophageal obstruction in a Morgan weanling colt was diagnosed by endoscopy, positive contrast radiography, and pleuroscopy. Surgical excision of the diverticulum alleviated clinical signs, and the horse was able to resume a normal diet by day 6. After 9 months the colt remains asymptomatic.     

Animal production evaluation of herbage varieties.

Perennial ryegrass/white clover (Lolium perennel Trifolium repens) pastures of three white clover varieties were compared at UK lowland (Plas Gogerddan, PG) and upland (Bronydd Mawr, BM) sites over three harvest years (1989–91) under continuous variable stocking (ewes and lambs until weaning and lambs thereafter). Mean annual lamb output from small-leaved S 184 (1179 kg ha^?1) was 29% greater than that from prostrate small-leaved AberEndura at PG with medium-leaved Huia also giving 19% more output than AberEndura. At BM, output from S 184 (863 kg ha^?1) was 19 and 14% greater than that from AberEndura and Huia respectively. The differences in lamb output between the small-leaved varieties owed to a combined effect of higher individual lamb growth rates and greater stock-carrying capacity, both of which were more pronounced in the post-weaning period. The higher output from S 184 relative to Huia at BM was attributed to higher stocking rate, particularly after weaning. Clover productivity and persistence were also studied under three cutting-only managements at PG. Performance under a treatment cut at 2–3 cm every 10 d (T1), which is synonymous with assessment of persistence in UK National List testing, was poor with clover growing point number of only 498 m^?2 compared with 4906 m^?2 on the grazed sward in autumn 1991. Although productivity and clover content under T3 (cut at 3–4 cm every 42 d — similar to National List yield regime) gave the same varietal ranking as lamb production, there was an under-estimation of the small-leaved varieties, particularly AberEndura, relative to Huia. An intermediate treatment (T2), cut at 3–4 cm every 21 d, also over-estimated the performance of the medium-leaved variety. Herbage data from ground level sampling every 21 d using exclosure cages on the grazed swards were also poorly related to lamb performance. These results highlight the effect of clover variety on lamb production, which appeared to be independent of leaf size, and also confirm the existing problems associated with assessment of white clover varieties using a cutting regime.     

Potential use of activated carbon as a protectant against pre-emergence herbicides in direct-seeded processing tomatoes

Activated carbon protected direct-seeded tomato (Lycopersicon esculentum Mill., cv. UC 82B) against pre-emergence applications of metribuzin and metolachlor, but not of oxyfluorfen or oxadiazon. Accurate placement of activated carbon on the soil surface directly above the seed was necessary for protection of tomato seedlings. Displacement of the carbon by overhead watering reduced its protective properties. A major disadvantage of the technique is that weed seeds under the activated carbon are also protected. Also, of the chemicals examined here, those against which it did provide protection did not control Solanum nigrum L. Utilisation potentielle du charbon actif comme phytoprotecteur des tomates conduites en semis direct contre les herbicides de prelevee Le carbone actif a protege des tomates en semis direct (Lycopersicon esculentum Mill., cv UC 82 B) contre des applications de prelevee de metribuzine et de metolachlor mais pas d'oxy-fluorfen ou oxadiazon. Une locahsation précise du charbon actif sur la surface du sol directe-ment au dessus de la graine est necessaire pour la protection des plantules de tomate. Le déplacement du charbon par arrosage réduit ses propriétés de protection. Un inconvénient important de la technique est que les graines d'adventices sous le charbon actif sont égale-ment protégées. En outre, les produits chimiques examinés ici qui assurent une protection, ne sont pas actifs contre Solanum nigrum L. Aktivkohle als Schutzmittel gegen Vorauflauf-Herbizide in gesdten Tomaten Mit Aktivkohle konnten gesate Tomaten (Lycopersicon esculentum Mill., ‘UC 82B’) gegen die Vorauflauf-Anwendungen von Metribuzin und Metolachlor geschutzt werden, jedoch nicht gegen Oxyfluorfen oder Oxadiazon. Fur den Schutz der Tomaten-Keimpflanzen mußte die Aktivkohle ganz genau uber den Samen auf die Bodenoberflache ausgebracht werden. Ver-schwammen der Kohle durch Beregnung reduzierte ihre Schutzwirkung. Ein großer Nachteil dieser Methode liegt darin, daß keimende Unkrauter unter der Aktivkohle ebenso geschutzt werden. Auch konnte mit den hier untersuchten Herbiziden Solanum nigrum L. nicht bekampft werden.     

Sperm Morphology Assessment in Captive Neotropical Primates

The main objective of this study was to evaluate sperm morphology in four neotropical primate species to compare the sperm morphological traits and the sperm morphometric parameters as a basis for establishing normative sperm standards for each species. Data from 80 ejaculates collected from four primate species, Callithrix jacchus, Callimico goeldii, Alouatta caraya and Ateles geoffroyi, were analysed for detection of sperm morphological alterations using subjective World Health Organization (WHO‐2010) standards and Sperm Deformity Index (SDI) criteria, objective computer‐assisted sperm morphometry analysis (CASMA) and subpopulation sperm determination (SSD) methods. There were multiple differences (p < 0.01) observed among primate species in values obtained from WHO‐2010, SDI, CASMA and SSD sperm analysis methods. In addition, multiple significant positive and negative correlations were observed between the sperm morphological traits (SDI, Sperm Deformity Index Head Defects, Sperm Deformity Index Midpiece Defects, Sperm Deformity Index Tail Defects, Normal Sperm, Head Defects, Midpiece Defects and Tail Defects) and the sperm morphometric parameters (SSD, Area (A), Perimeter (P), Length (L), Width (W), Ellipticity, Elongation and Rugosity) (p ≤ 0.046). In conclusion, our findings using different evaluation methods indicate that pronounced sperm morphological variation exists among these four neotropical primate species. Because of the strong relationship observed among morphological and morphometric parameters, these results suggest that application of objective analysis methods could substantially improve the reliability of comparative studies and help to establish valid normative sperm values for neotropical primates.     

Serological survey of exposure to Erysipelothrix rhusiopathiae in poultryin New Zealand

Abstract

AIMS: To estimate the seroprevalence of antibodies to Erysipelothrix rhusiopathiae in chickens in New Zealand, and to estimate the effect of housing type, geographical location and age on seroprevalence.

METHODS: A cross-sectional serological survey of a convenience sample of 545 broiler, breeder, and layer chickens in 55 flocks was conducted in 2010–2011. Birds were aged 5–83 weeks; housing types were free-range, shed, caged, and unknown; and flocks were located in the Auckland, Manawatu, North Canterbury, Otago, Taranaki, Waikato, and Wairarapa regions of New Zealand. An ELISA was used to measure antibodies to E. rhusiopathiae. Samples with an optical density reading ≥1.50 were considered to be positive. Logistic regression analysis was used to model the effect of housing type, geographical location and flock age on the prevalence of samples positive for antibodies to E. rhusiopathiae.

RESULTS: The overall prevalence of samples with antibodies to E. rhusiopathiae was 39.8 (95% CI=35.68–44.06)% for the 545 samples, and 46/55 (84%) farms that were tested had at least one positive sample. Mean seroprevalence for types of housing was 44.2 (95% CI=37.79–50.70)% for free-range (n=240 birds), 23.7 (95% CI=17.83–30.38)% for shed (n=190), 73 (95% CI=56–86)% for caged (n=37) and 50 (95% CI=38–62)% for unknown (n=78). The disease was present in all seven geographical locations from which samples were obtained for this study. Seroprevalence increased with increasing age of birds (p<0.001); for birds ≤12 weeks of age it was 2 (95% CI=0.3–8)% (n=91), 13–24 weeks 29.1 (95% CI=23.34–35.46)% (n=230), 25–36 weeks 47 (95% CI=32–64)% (n=40), 37–48 weeks 75 (95% CI=51–91)% (n=20), >48 weeks 63.8 (95% CI=54.78–72.12)% (n=127). Neither housing type nor geographical location had a significant effect on the likelihood of samples being positive for antibodies to E. rhusiopathiae.

CONCLUSIONS: The results of this study suggest the prevalence of erysipelas under New Zealand field conditions may be higher than expected and that the disease is significantly associated with increasing age. Housing type and geographical location appear to be unrelated to seroprevalence.

CLINICAL RELEVANCE: Further study of the epidemiology of E. rhusiopathiae in chickens in New Zealand should be considered in order to minimise the extent of birds' exposure to the organism. These findings will assist in the design of further studies.     

Comparison of the Johne''s absorbed EIA and the complement-fixation test for the diagnosis of Johne''s disease in cattle

A commercially available absorbed ELISA for the diagnosis of Johne's disease (JD) (paratuberculosis) in cattle, the Johne's Absorbed EIA, was compared with the conventional complement-fixation test (CFT) used in Australia. Stored plasma from 3 Victorian dairy herds with a history of JD, sera from specimens submitted from animals showing clinical signs of JD and sera from the US National Repository for Paratuberculosis Specimens were used to determine the sensitivity of each test. The EIA detected 48.8% of 43 Australian animals with subclinical JD, while the CFT detected only 12 (21.4%) of 56 subclinically affected cattle. Of 150 subclinically infected US cattle, the EIA detected 47.3% and the CFT detected 52.0%. The EIA detected 59.7% of animals which at the time of sampling were shedding Mycobacterium paratuberculosis in their faeces, but showed no clinical signs of JD, while the CFT detected 57.3%. The EIA correctly identified 88.2% of 136 histologically confirmed clinical cases, and the CFT detected 83.4%. The specificity of each test was determined by testing sera collected at slaughter from animals residing in a known JD-free area of Australia, and from samples from the US National Repository of Paratuberculosis Specimens collected from certified-free herds in Wisconsin. The EIA was found to have a specificity of 99.8% when 998 Australian animals were used as the test population, and 99.0% when 196 US animals were used. The specificity of the CFT using Australian samples was 96.9% and 95.2% using American samples.     

Variation in spring temperatures, grass production and response to nitrogen over twenty years in the uplands

Large between-year variation was observed in spring air and soil temperatures at Pant-y-dwr Hill Centre (305 m altitude) from 1967 to 1984 and at Bronydd Mawr Research Centre (330 m) from 1985 to 1986 in Powys. The mean date of attaining T-sum 200°C accumulated air temperature was 13 March (range 9 February to 23 April) and that of soil temperature at 100 mm depth permanently above 5 5°C was 9 April (range 7 March to 4 May). Net herbage accumulation and response to applied N from Aberystwyth S23 perennial ryegrass (Lolium perenne L.) swards during April were also highly variable and were correlated with the date of reaching target soil temperatures of above 5 5°C for 5 consecutive days ( r =−0·68, P < 0·001 for net herbage accumulation and r =−0·70, P < 0·001 for response to N).
The use of early flowering varieties of perennial ryegrass gave a significant increase of herbage growth in spring compared with late varieties. From 1979 to 1980 net herbage accumulation during April from the early variety Frances was 94% more than from Perma (late) and 55% more than from Talbot (intermediate) varieties. Under conservation management more winter hardy and persistent varieties of Italian and hybrid ryegrasses ( L. multiflorum L. and L. multiflorum X L. perenne ) gave higher quantities of firstcut silage crops in early June than RvP Italian ryegrass.
After the severe winters of 1978–79 and 1985–86, subsequent spring production from a wide range of ryegrasses was shown to be affected by sward survival, highlighting the value of winter hardy varieties when resowing in the uplands     

A LUMBOSACRAL TRANSITIONAL VERTEBRA IN THE DOG PREDISPOSES TO CAUDA EQUINA SYNDROME

The association between the occurrence of a lumbosacral transitional vertebra (LTV) and the cauda equina syndrome (CES) in dogs was investigated. In 4000 control dogs without signs of CES, 3.5% had an LTV, while in 92 dogs with CES, 16.3% had an LTV. The lesion causing CES always occurred between the last true lumbar vertebra and the LTV. Dogs with an LTV were eight times more likely to develop CES than dogs without an LTV. German Shepherd dogs were eight times more likely to develop CES compared with other breeds. Male dogs were twice as likely to develop CES than females. Dogs with an LTV develop CES 1-2 years earlier than dogs without an LTV.