淡水养殖水化学实验课教学改革

为了培养学生的实验技能和科研意识,提高综合能力,笔者对淡水养殖化学实验课教学改革展开探讨。目前淡水养殖化学实验课主要存在实验项目部分重复,实验试剂涉及一些贵重的、有毒的药品等问题。因此,在考虑到不重复设置实验项目及尽量避免有毒试剂大量使用的前提下,笔者结合农业分析测试中心的大型精密仪器设备,尝试实验课的教学改革,注重引进大型精密仪器,整合实验项目,向学生灌输环保安全的理念,培养学生的科研素质,提高学生的综合能力。     

减蛋综合征病毒环介导等温扩增检测方法的建立

根据GenBank登录的减蛋综合征病毒(EDSV)六邻体基因的保守区,设计了3对环介导等温基因扩增(LAMP)引物,优化反应体系,并对其灵敏性、特异性进行验证.结果表明:LAMP优化后的反应体系为外引物浓度0.1μm01·L-1,内引物浓度0.8 μmol·L-1,环引物浓度0.4μmol·L-1,Mg2+浓度8.0 ...     

A comparison of the pathogenicity of two strains of hog cholera virus: 1. Clinical and pathological studies

The virulence of a strain of hog cholera virus isolated during an outbreak of mild disease in pigs in New South Wales in 1960/61 (the NSW strain) was compared over 11 days with that of a virulent strain by inoculating 8 pigs with each virus and comparing the ensuing clinical signs and pathology. Both viruses caused persistent pyrexia and leukopenia, the NSW strain 4 to 5 days and the virulent strain 3 days, after inoculation. Few other clinical signs were observed in the pigs inoculated with the NSW strain. In contrast, all pigs inoculated with the virulent strain became progressively depressed and incoordinated, and were killed between days 6 and 9. Bronchopneumonia and swollen, reddened lymph nodes were observed in pigs inoculated with both viruses. Few other gross lesions were observed with the NSW strain, but some pigs receiving the virulent strain had pustules in the tonsil and the anterior oesophagus, petechial haemorrhages in the kidney, and small infarcts at the margins of the spleen. There were marked differences in the histopathology, both in the variety of organs affected and the severity of lesions in individual organs. Suppurative bronchopneumonia occurred in both groups. Other changes in the pigs affected with the NSW strain were colitis, mild cerebral vasculitis, necrosis, haemorrhage and neutrophil infiltration in some lymph nodes and spleens. In pigs infected with virulent virus the cerebral vasculitis was so severe that there was necrosis of cells within the vessel walls.(ABSTRACT TRUNCATED AT 250 WORDS)     

Susceptibility of cats to equine morbillivirus

Objective To assess the susceptibility of cats to equine morbillivirus (EMV) by direct administration of the virus by subcutaneous, intra-nasal or oral routes, and following exposure to infected cats.
Design A disease transmission study, with controls, using ten cats.
Procedure Groups of cats were given the virus by the designated methods and assessed for evidence of infection by clinical examination, plus pathological and virological tests.
Results All cats administered the virus by subcutaneous, intra-nasal or oral routes became infected and developed the disease within 4 to 8 days. One of two cats in contact with affected cats also developed the disease, but two cats kept near to affected cats did not become infected. The virus was isolated from a range of tissues collected from the infected cats, and the lesions observed in affected cats were similar to those previously observed in horses naturally and experimentally infected with the virus.
Conclusion This is the first demonstration that animals can be infected with EMV by non-parenteral means, that the virus can transmit naturally between animals and confirms other reports of the similarity of EMV disease in horses and cats.     

鸡公山自然保护区森林生态系统服务功能及其价值初步研究

从生态系统服务功能的概念入手,根据调查和研究,采用替代工程、市场价值等方法,从2个方面评价了鸡公山自然保护区森林生态系统服务功能的价值.结果表明,面积3000hm2的鸡公山自然保护区森林生态系统的生态服务功能价值70548.6万元·a-1,其中涵养水源价值8593.9万元·a-1,净化水质价值1265.3万元·a-1,保持土壤价值57754.1万元·a-1,固碳制氧价值2114.2万元·a-1,净化环境价值(吸收SO2量)26.1万元·a-1,游憩价值550万元·a-1,林产品价值245万元·a-1.     

Number of Follicular Waves and Their Effect on Pregnancy Rate in the Cow

In the study, the relationship of follicular growth waves, oestradiol and pregnancy rates were investigated during oestrus cycle in cows. A total of 22, Brown Swiss cows (3-5 years old) were used for the study. The ovaries of animals were examined from sixth day of cycle to next oestrus by ultrasound. The follicles that were present in the ovarium were recorded. Follicular growth was observed every day by means of ultrasound examination. The blood samples were taken for analysis of oestradiol simultaneously with ultrasound examinations. The oestrus animals were inseminated. Each animal that inseminated was examined by ultrasound on day 28 after insemination for pregnancy diagnosis. Two follicular growth waves were observed in nine of 22 (40.9%), three waves in 13 of 22 (59.1%) animals. The oestradiol was found in the same concentration but in different release patterns between two and three waves animals. Pregnancy rate in cows with three and two follicular waves did not differ. In conclusion, emergence of three waves of follicular growth was higher in Brown Swiss cows, the analysis of oestradiol could be used for determination of the wave numbers and the animals with different waves may have had the same pregnancy rates.     

草坪草种(品种)的筛选及利用

以绿色期、质量、抗逆性及建植效率为指标 ,对国内外的优良草坪草进行了引种筛选工作。筛选出了适宜内蒙古气候条件的 9个品种 ,培育出了冷季型草坪草品种—大青山草地早熟禾。同时对优良的地被植物进行了引种驯化     

烷基锡酸催化松香酯化反应的研究

以松香树脂酸的酯化反应为例，研究了烷基锡酸为酸化反应的催化作用，系统的探讨了影响反应的各种因素，研究表明烷其锡酸具有很高的催化活性，可以高效地催化合成系列松香酯，大大缩短了反应时间。     

传染性胰腺坏死病毒VP3蛋白的原核表达及抗原性分析

应用RT-PCR方法扩增了IPNV编码内衣壳VP3蛋白的基因615bp,将VP3基因克隆至原核表达载体pET30b,并在大肠杆菌BL21中得到了表达。通过SDS-PAGE分析表明,重组菌诱导后得到了预期大小约30ku的VP3蛋白,与理论值相符,经薄层扫描分析表明目的蛋白表达量可占菌体总蛋白的30%。用镍离子亲和层析柱纯化可溶性的VP3蛋白,并制备抗血清。Western-blotting结果显示,VP3蛋白可被兔抗IPNV阳性血清识别;间接ELISA结果显示,IPNV细胞培养物作为抗原,兔抗VP3蛋白高免血清稀释度为1∶25600时,P/N>2,抗血清可与IPNV全病毒发生反应,以上两项结果说明,表达的VP3蛋白与天然的IPNVVP3蛋白一样具有相同的抗原性。试验利用原核表达系统成功地高效表达了IPNVVP3蛋白,融合蛋白以可溶性形式存在,并制备了高效价的抗血清。     

控制辐照食品安全的HACCP质量管理体系

介绍了食品危害分析与关键控制点体系 (HACCP) ,对脱水蔬菜辐照灭菌过程中的各个环节可能造成的潜在危害进行了分析 ,并探讨了在中国辐照食品加工企业中建立HACCP的方法