Role of gap junction in pentylenetetrazol-induced epilepsy in rats

AIM: To study the role of gap junction(GJ) in the pathogenesis of epilepsy. METHODS: The expression of connexin(Cx) 32 and Cx43 was evaluated in penlylenetetrazol(PTZ)-induced epilepsy rats at different time points with immunohistochemistry and real-time RT-PCR. The uncoupler of gap junction,carbenoxolone (CBX), and anti-epilepsy drug,carbamazepine (CBZ), were introduced to investigate the role of gap junction in epilepsy. RESULTS: The expression of Cx32 in the cortex and hippocampus of rats increased 2 h after PTZ administration, and was more obvious 8 h later, so was the expression of Cx43. CBX not only notably inhibited the expression of Cx32 and Cx43, but also suppressed the seizures. CBZ had no obvious effect on Cx32/43 expression. Real-time RT-PCR examination showed that the level of Cx32 mRNA went up rapidly 2 h after seizures, and began to decrease 10 h later. The level of Cx43 mRNA in PTZ group was higher than that in control group from 2 h to 5 h. CBX inhibited both the seizures and the increase in the mRNA expression of Cx32/43, while CBZ only suppressed the seizures and did not affect the expression of Cx32/43. CONCLUSION: GJ between neurons is reinforced after epileptic activities and takes part in the pathophysiological mechanism of synchronization and epilepsy. CBZ has no effect on the expression of Cx32 and Cx43, indicating that the anti-epileptic mechanism of CBZ is independent of GJ.     

叶面施锌对苋菜生长的影响

采用0.28、0.40和1.00ml.L-1的锌肥禾丰锌〔氧化锌≥87%(W/V)〕进行叶面施肥,研究叶面施锌对苋菜生长的影响。结果表明:3个施锌处理均对苋菜株高、茎粗和叶面积有促进作用,并能改善苋菜品质,降低硝酸盐积累,增加产量,其中浓度为0.40ml.L-1的处理效果最佳。     

猕猴桃实生苗组织培养体系建立的研究

以饱满成熟的猕猴桃种子为起始材料,用 2. 5g·L-1赤霉素处理 5小时,再用次氯酸钠灭菌消毒后,将萌芽的种子接种到MS+蔗糖 20g·L-1 +肌醇 100mg·L-1和 0. 75%琼脂的培养基上,根、茎、叶生长表现良好,初步建立了猕猴桃实生苗组织培养体系。猕猴桃实生苗组织培养同利用茎段、茎尖组织培养一样,培养基中无须附加任何激素。     

红托鹅膏生态环境研究

以武定狮山为样点,对红托鹅膏的生境进行了初步研究。结果表明,红托鹅膏主要生长于滇青冈、滇石栎等阔叶林或针（云南松）阔混交林中,其生长的土表层疏松、透气、潮湿,具有厚4 cm~9 cm的腐叶层,有稀薄的阳光透入,土层有机质含量高,矿物质养分丰富,土壤容重较小,偏微酸性（pH5.4~5.7）。子实体分化发育较适宜的温度为19℃~21℃,空气湿度为75%~86%,土壤含水量为38%~50%,并每天保证1 300 lx~2 000 lx的光照2 h~5 h,200 lx~900 lx的弱光6 h~9 h。     

Expression of natural killer cell stimulatory receptor NKG2D and its ligand MICA in Tibetan patients with gastric cancer at Qinghai plateau

AIM: To investigate the expression of natural killer cell stimulation receptor D(NKG2D) in peripheral blood and the expression of major histocompatibility complex class I chain-related protein A(MICA) on the gastric carcinoma tissue and the neighboring non-cancerous tissue in Tibetan patients with gastric cancer at Qinghai plateau. METHODS: Samples of 33 patients and 20 healthy persons were collected to detect NKG2D in peripheral blood by Flow cytometry analysis. The expressions of MICA in part of the correspondent tissues were examined by RT-PCR and immunohistochemistry.RESULTS: The expression of NKG2D in the plateau of Tibetan patients with gastric cancer group (13.47 ±5.26)% was significantly lower than that in healthy groups (32.62±10.08)%. The mRNA level of MICA in gastric cancer was significantly higher than that in neighboring non-cancerous tissue (P<0.05). The expressions of MICA proteins showed a significant difference between the neighboring non-cancerous tissue (21.21%, 7/33) and the gastric carcinoma tissue (78.79%, 26/33), between the high differentiation (60.00%, 3/5) and the moderate (72.73%, 8/11), low differentiation (88.24%, 15/17). The expressions of MICA were not correlated with tumor size, sex, age, lymph node metastasis of the patients (P>0.05). Spearman rank correlation displayed that the expressions of MICA mRNA were positively correlated with the MICA protein level (r=0.903, P<0.01).CONCLUSION: The immune escape in Tibetan patients with gastric cancer at Qinghai plateau probably relates to the down-regulation of NKG2D and the up-regulation of its ligand MICA. The activity of NK cells and the anti-cancer cellular immunity level are descending in the plateau of Tibetan patients with gastric cancer. The decrease in the receptor NKG2D is a reason for the activity of NK cell descending.     

早熟马铃薯新品种洛马铃薯8号的选育

洛马铃薯8号由“中薯3号x秦芋30号”杂交选育而成。早熟,生育期66d,休眠期中等,51d左右。薯块卵圆形,黄皮黄肉,薯皮光滑,芽眼浅,薯块较整齐,平均单株薯块数2．5个,单薯质量104．72g,商品薯率94．46％,淀粉含量13．6％,VC含量24．4m~100g,蛋白质2．70％,还原糖含量0．19％,较抗病毒病和晚疫病．667m2产量1302．04kg以上,适宜中原二季作区春秋两季种植。     

侧柏叶总黄酮对槐蚜的杀虫活性研究

用乙醇水加热回流法提取侧柏叶总黄酮,并就提取物对槐蚜的杀虫活性进行了测定.结果表明:室内叶片法生测后,提取物对槐蚜毒效的LC50为13.71 mg/mL;田间防治效果测定,当浓度为30 mg/mL时,对槐蚜的防治效果为85.19％.因此,侧柏叶总黄酮对槐蚜有较好的杀虫效果.     

山东潍坊市园林植物病虫害可持续控制研究

对潍坊园林植物主要病虫害进行调查,分析其发生特点,并提出相应对策:强调以生态学为基础,以生物防治为主导,综合采用植物检疫、栽培管理、化学防治等方法,达到对病虫害可持续控制的目的。     

三种灌木的蒸腾耗水特性研究

利用Lcro+便携式光合仪,对青岛市3种主要灌木树种连翘、金银木、锦带花的蒸腾耗水规律进行了测定和研究。结果表明:在水分充足条件下连翘呈典型的双峰曲线,金银木和锦带花呈单峰曲线;金银木在1 d的各时间段耗水速率明显高于连翘和锦带花的耗水速率;3种灌木晴天的耗水速率明显大于阴天的耗水速率;此外,在春季,连翘对土壤含水量比金银木、锦带花敏感。试验的结果能够为植物的合理选择、水分的量化管理提供科学依据。     

三种香料蔬菜的营养及挥发性物质分析

对百里香、罗勒、龙蒿草3种香料蔬菜的营养物质含量及挥发性化学物质成分进行了分析,并与普通蔬菜的文献值进行比较。结果表明:3种香料蔬菜的蛋白质、碳水化合物及无机营养元素钙、铁、锌和硒、维生素C含量比普通蔬菜高,而脂肪、胡萝卜素含量与普通蔬菜相比无明显差异。