高温和干旱胁迫下,植物叶片光合系统变化、气孔运动及其调控研究进展

全球气候变暖导致灾难性天气发生频率增高,高温和干旱同时发生的几率逐年增加,针对高温和干旱给植物带来的影响,本文从植物叶片光合系统、气孔运动、逆境胁迫信号传导和叶片气孔开闭调控4个方面进行详细阐述,从植物生理学和细胞生物学角度,讨论了植物叶片气孔运动规律及应用脱落酸对气孔开闭进行调控的研究进展,为抗旱耐高温型植物新品种选育提供理论基础,为科学、经济、高效调控植物叶片气孔运动,防御短暂和长期性逆境胁迫,保证粮食稳产、高产提供科学依据。     

鸡白细胞介素18研究进展

鸡白细胞介素(IL-18)是一种新发现的细胞因子,其cDNA全长597 bp,编码198个氨基酸的前体多肽,分子量约为23 kDa,IL-18氨基端的29个氨基酸残基起胞内定位作用;鸡IL-18是IFN-γ诱生剂,通过IFN-γ发挥抗感染、促生长等作用,参与对微生物感染诱导产生细胞和体液免疫反应的初次免疫应答;鸡IL-18作为免疫佐剂,具有增强免疫和治疗作用,还具有抗肿瘤免疫作用。     

Analysis on the Variation Expression of Difficult-Dissolved-Proteins of Diapause Eggs After Activating by Acid Treatment

Using HCl to activate the diapause eggs is a traditional technique of artificial hatching applied in silkworm egg production. Its mechanism has not yet been clarified. This experiment explored the effect of HCl on the termination of diapause of silkworm eggs cold-stored for 45 days from the point of proteomes. Two-dimensional electrophoresis techniques and ESI- MS-MS were used to compare and analyze the variation expression of difficult-dissolved-proteins of diapause eggs coldstored for 45 days before and after acid treatment. Through analysis on the two-dimensional electrophoretogram, there were 296 dots before acid treatment and 302 after the treatment, respectively. Amongst them 265 dots were matchable. The matchability reached 88.6%. There were 31 specific protein dots before acid treatment and 37 after acid treatment, respectively. ESI-MS-MS analysis was conducted for two specific protein-rich dots which disappeared after acid treatment. The results indicated that the sequence of No. 1 protein dot had 55 amino acids＇ peptide matched with those of chorion protein （Bombyx mori）. While the sequence of No. 2 protein dot had only 15 amino acids＇ peptide matched with those of heat shock protein hsp 19.9 （Bombyx mori）, and it was presumed to be an unknown protein. The difficult-dissolvedproteins of diapause eggs have variation expression after acid treatment. Some proteins before and after acid treatment are changed in MW.     

应用RACE法分离和克隆猪GPX2基因研究（英文）

[Objective] Using molecular biotechnology to clone the sus scrofa GPX2 gene.[Method] Using total RNA of sus scrofa duodenum as template,degenerated primer pairs were designed according to the homology alignment analysis of GPX2 gene of human,rat,mouse,dog and cattle.A sus scrofa GPX2 gene sequence of 330 bp was obtained by RT-PCR application method.Primes were designed respectively according to the known sequence,sus scrofa GPX2 gene was isolated and cloned by 3-RACE and 5-RACE method and analyzed the gene sequence.[Result] A mRNA sequence of 924 bp was successfully cloned and isolated in this research.This sequence contained complete 3' end and had higher sequence homology with human,mouse,cattle and dog GPX2 gene,and there was codon called TGA which encoding Sec on the position of No.114-116 gene.[Conclusion] Sequence alignment analysis showed that the cloned gene was sus scrofa GPX2 gene(NCBI GenBank database,the sequence number was DQ98982).     

新疆畜牧科技信息服务平台建设

农业信息化建设是社会主义新农村建设的重要内容之一,畜牧业信息化是其主要组成部分.本文通过对新疆畜牧业信息服务平台现状和存在问题进行分析,系统阐述了新疆畜牧科技综合信息服务平台建设的总体思路和特点.该平台的建设,将为新疆畜牧业的发展起到积极的推进作用.     

航天育种机理的研究进展

阐述了航天育种机理的研究进展。对航天育种的主要因素—强辐射与微重力的来源、对植物种子的作用机理和所产生的生物效应进行了重点阐述,并指出了其研究的不足和发展方向。     

一种带有封闭气腔的保温板研制

建筑屋面的保温和隔热处理是建筑节能的一个重要内容,为此研制了一种带有"封闭气腔"的水泥珍珠岩保温板.应用空气间层的保温与隔热原理,通过多层匀质材料组成的维护结构的传热方面的理论计算,确定了该保温板的制作工艺为特殊定做专用成型气袋施工法;材料选择论证表明,封闭气腔与水泥珍珠岩板结合是一种理想的保温隔热材料.     

1-MCP处理对猕猴桃果实采后生理的影响

以秦美猕猴桃为试材,在常温下(20±0.5℃),研究了不同浓度1-甲基环丙烯(1-MCP)对果实采后生理的影响。结果表明,与对照相比,1-MCP处理显著抑制了果实乙烯的释放量,有效地延迟了果实的软化,对果实可溶性固形物含量的影响不明显,一定程度上提高了果实的贮藏效果。     

基于GIS和虚拟现实技术的驾驶训练、绩效测评系统开发

文章分析了基于人因工程学原理构建虚拟驾驶训练、绩效测评系统的特点,系统运用人因工程学原理设计,人机交互界面友善、交互性强、可视化效果增强,探讨了系统的软硬件结构及作用机理,给出了系统开发思路及流程-模型数据获取、数据处理、Creator建模、Vega程序设计和系统测试,应用GIS、虚拟现实技术和虚拟GIS技术建立了集汽车驾驶虚拟训练、驾驶员操作绩效数据获取、交通事故重现于一体的多功能虚拟系统。     

Enzymatic activity and chlorophyll fluorescence imaging of maize seedlings (Zea mays L.) after exposure to low doses of chlorsulfuron and cadmium

The aim of this research was to study the influence of chlorsulfuron residue and cadmium on the enzymatic activity and photosynthetic apparatus of maize(Zea mays L.) plants. Chlorsulfuron and cadmium at 0.001 and 5.0 mg kg–1, respectively, were mixed and applied to soil prior to planting. The levels of chlorsulfuron-and cadmium-induced stress to plants were estimated by growth, chlorophyll content, lipid peroxide content, enzyme activities, and major fluorescence parameters of chlorophyll(revealed by the fluorescence imaging system Fluor Cam). Chlorsulfuron negatively affected the chlorophyll content, photochemical efficiency of photosystem II in the dark-adapted state, the maximum efficiency of photosystem II, photochemical quenching coefficient, and steady-state fluorescence decline ratio in the leaves of maize seedlings. However, cadmium did not produce noticeable changes. Plants that were exposed to both chlorsulfuron and cadmium showed an obvious increase in the steady-state fluorescence decline ratio. These results implied that the seedlings possessed more resistance to cadmium than to chlorsulfuron and their resistance to chlorsulfuron toxicity was enhanced by the presence of cadmium. The results also suggested that chlorophyll fluorescence imaging reveals overall alterations within the leaves but may not reflect small-scale effects on tissues, as numeric values of specific parameters are averages of the data collected from the whole leaf.