Kan浓度对无菌苗生根影响的研究

在未加激素的MS培养基中添加不同浓度的Kan,研究其对桑树陕305无菌苗的生根抑制程度,确定转基因植株延迟筛选的适宜浓度。培养基中添加Kan6mg/L是延迟筛选的适宜浓度。     

Comparison Analysis of Immune Related Gene of Orf Virus in Sheep and Camels

The purpose of this study was to investigate the variation of Orf virus (ORFV) immune related genes after infection with different species.The ORFV genomes of sheep and camel were extracted and named ORFV-Y and ORFV-LT,respectively.Based on ORFV genome sequence published in GenBank (accession No.:KF234407.1),three pairs of specific primers were designed and synthesized to amplify the B2L,F1L and VIR gene fragments of ORFV-Y and ORFV-LT,respectively,and the amplified fragments were cloned into pMD19-T vector,transformed into E.coli DH5α competent cells.The recombinant plasmid was identified,and positive clones were selected for sequencing,DNAStar software was used to analyze the homology,amino acid sequence and phylogenetic tree of 13 ORFV genome sequences published on NCBI.The results showed that the nucleotide homology of B2L,F1L and VIR genes were 92.8% to 99.2%,95.7% to 99.5% and 77.6% to 100%,respectively.After comparing the amino acid sequence between the two genomes and the reference sequence,it was found that there were obvious differences in the immune related genes between the two genomes,and F1L gene had some rules to follow.The phylogenetic analysis of B2L,F1L and VIR genes showed that ORFV-Y was closely related to the Chinese Fujian goat strain,while ORFV-LT was far from the reference strains,and was a separate branch.The results showed that ORFV had obvious difference in immune related genes between sheep and camels,it provided a reference basis for further research on the changes of ORFV gene sequences in different species and the development of vaccines for different species in the future.     

一株基因Ⅶ型新城疫病毒的分离及分子鉴定

从一起新城疫暴发的病例中分离出1株新城疫病毒（NDV），命名为铜山分离株（TS）。运用RT-PCR技术，克隆了该分离株F基因的重要功能片段（约0.5kb)，并进行了序列测定。序列分析表明，TS分离株在F蛋白裂解位点的氨基酸顺序为^112-R-R-Q-K-R-F^117，与NDV强毒株特征相符，且具有101外的K（赖氨酸）和121处V（缬氨酸）2个特征性氨基酸，与基因Ⅶ型NDV的特性完全相符。     

Association of tumor budding with tumor-infiltrating lymphocytes and prognosis in breast cancer patients

AIM: To investigate the relationship of tumor budding with clinicopathologic parameters, tumor-infiltrating lymphocytes (TILs) of tumor microenvironment and the prognosis in breast cancer patients.METHODS: A total of 178 HE section samples were collected from the breast cancer patients treated with surgery in the First Affilated Hospital of Jinan University during Jan. 2012 to Dec. 2016. The tumor budding and stromal tumor-infiltrating lymphocytes were observed under light microscope. The correlation of tumor budding with the clinicopathologic status and TILs were analyzed by χ² test. Kaplan-Meier survival analysis and Log-rank test were used to estimate the disease-free survival (DFS) and overall survival (OS).RESULTS: High tumor budding level was associated with more positive lymph nodes, higher grade, and more lymphovascular invasion. In addition, the patients with higher tumor budding level showed fewer TILs, while the patients with lower tumor budding level had more TILs. Furthermore, the patients with higher tumor budding level had a worse disease-free survival and overall survival than those with lower tumor budding level.CONCLUSION: Tumor budding is significantly associated with adverse clinicopathological characteristics of breast cancer and negatively correlated with TILs. Therefore, tumor budding may serve as a potential biomarker to predict the prognosis of breast cancer.     

氨基酸添加方式和营养液杀菌方式对生菜产量与品质的影响

研究了生菜水培过程中,用混合氨基酸替代20％硝态氮时,不同的氨基酸添加方式和营养液的杀菌方式对生菜产量和品质的影响。试验于生菜采收前12 d开始进行,试验设置4个处理:A．替代氨基酸1次全部加入;B．每3 d添加氨基酸替代总量的1／4,共添加4次;C．氨基酸分次添加(添加方式同B处理),每次添加前营养液通臭氧0．5 h杀菌; D．氨基酸分次添加(添加方式同B处理),每次添加前把营养液加热到60-70℃保持10 min进行杀菌,然后迅速把营养液温度降低到常温。结果表明,在不杀菌的情况下,1次添加的产量和品质优于分次添加,但在降低硝酸盐含量方面,分次添加优于1次添加;在分次添加的情况下,杀菌与不杀菌相比,杀菌可以明显提高生菜的产量和品质,加热杀菌对生菜产量和品质的影响优于臭氧杀菌,臭氧杀菌处理的生菜根系活力、叶绿素等指标高于加热杀菌,但2种杀菌方式之间差异不大。     

Cu2O掺杂技术研究进展

氧化亚铜(Cu2O)作为理想的低成本太阳电池材料,因其表面阻抗过大导致在实际应用中转换效率偏低.如何提高Cu2O电导性已经成为Cu2O光伏应用领域研究的关键.掺杂技术作为一种控制半导体导电类型和电阻率常见的做法,被越来越多的应用于Cu2O光伏材料制备的研究.文章综述了近年来Cu2O掺杂技术的研究进展,为高效率Cu2O基太阳电池的研究提供了参考.     

恢复生态学的理论与研究进展

为了解西江流域广西境内卷口鱼 (Ptychidio jordani) 种群遗传结构及分化程度,采用线粒体Cytb基因序列对西江流域广西境内6个江段的139尾野生卷口鱼的遗传多样性进行了分析。结果显示,线粒体Cytb基因长度为1 053 bp,碱基T、C、A、G的平均含量分别为29.1%、27.7%、29.3%、13.9%,其中A+T (58.4%) 高于C+G (41.6%)。共定义20个单倍型,并聚为2个分支,未观察到明显的地理聚群。6个卷口鱼群体的平均单倍型多样性和平均核苷酸多样性分别为0.7682、0.0023,其中红水河群体 (单倍型多样性h=0.748 7,核苷酸多样性π=0.003 3) 遗传多样性最高,柳江群体 (h=0.274 4, π=0.000 4) 和左江群体 (h=0.3747, π=0.000 3) 的遗传多样性相对较低。卷口鱼总体的遗传分化指数 (F_ST) 为0.461 4 (P<0.01),表现出较大的遗传分化。两两群体间遗传分化结果显示,左江和柳江种群之间的遗传分化程度最大,而柳江和西江之间最小。AMOVA分析表明西江流域的卷口鱼群体遗传变异一半来自群体内 (53.86%),一半来自群体间 (46.14%)。中性检验 (Tajima's D=−1.082 8, P>0.05;Fu's Fs=−6.572 5, 0.01<P<0.05) 与碱基错配分布分析表明西江流域卷口鱼种群大约在0.07~0.187 Ma经历了种群扩张。综上,西江流域广西境内的卷口鱼柳江群体和左江群体遗传多样性较低,总群体分化程度较大,但仍属于一个种群,其中空间距离与地理阻隔对卷口鱼的遗传分化具有一定的促进作用。

     

响应面法优化Xenorhabdus nematophila发酵培养基的研究

【目的】提高Xenorhabdus nematophilaYL001的抗菌活性,为该菌株杀菌活性成分的提取分离及生物农药的开发应用奠定基础。【方法】以YSG培养基为基础,采用单因子试验方法,对最佳碳源和氮源进行筛选,并采用全因子中心组合试验设计和响应面法对其最佳配比进行优化。【结果】YL001菌株的最佳碳源为玉米粉,氮源为豆饼粉,培养基的最佳组成为:玉米粉9.69 g/L,豆饼粉76.50 g/L,MgSO41.07 g/L,(NH4)2SO41.79 g/L,KH2PO40.63 g/L,K2HPO40.80 g/L,Na2SO41.25 g/L,在此条件下,YL001菌株抗菌活性达268.9 U/mL。【结论】培养基优化后YL001菌株的抗菌活性与试验的预测值接近,表明响应面法在培养基优化中十分有效,相对简单,且节省时间和材料。     

假俭草抗寒性与体内碳水化合物、脯氨酸、可溶性蛋白含量的关系

为了阐述假俭草(Eremochloa ophiuroides(Munro.)Hack.)的抗寒性与碳水化合物、脯氨酸、可溶性蛋白含量季节动态变化之间的关系,于2008年10月-2009年5月,研究了在自然温度变化过程中,假俭草E126匍匐茎中碳水化合物、脯氨酸、可溶性蛋白的季节性动态变化及其与半致死温度(LT₅₀)变化的关系。结果表明:在越冬前后假俭草E126匍匐茎LT₅₀呈现先降低后升高的季节变化趋势,可溶性总糖、蔗糖、果糖含量,可溶性总糖淀粉比率以及脯氨酸含量伴随田间气温变化均呈现先升高后降低的趋势,可溶性蛋白、淀粉含量则呈现出先降低又升高的趋势。假俭草E126匍匐茎LT₅₀季节变化与可溶性总糖、蔗糖、果糖含量,可溶性总糖淀粉比率季节变化之间存在极显著负相关(P<0.01),而与淀粉含量、脯氨酸含量、可溶性蛋白含量的季节变化间相关性不显著。因此,可溶性总糖含量、蔗糖含量、果糖含量、可溶性总糖淀粉比率对其冬季抗寒性增强有重要作用。     

中国西藏小反刍兽疫的发生状况与防控

2007年7月,我国首次在西藏自治区阿里地区发生了小反刍兽疫疫病流行.从2007年7月9日到11月15日,先后在阿里地区革吉、日土、札达、改则4个县出现疫情,共20个疫点,羊发病6122只,死亡1888只.2008年6月,在那曲地区尼玛县双湖区再次发现小反刍兽疫疫情,病羊及同群羊合计6690只.为消灭该病,采取了扑杀、...