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991.
Soil and root samples were collected from major tomato growing areas of Ethiopia during the 2012/2013 growing season to identify root-knot nematode problems. DNA-based and isozyme techniques revealed that Meloidogyne incognita and M. javanica were the predominant Meloidogyne species across the sampled areas. The aggressiveness of different populations of these species was assessed on tomato cultivars Marmande and Moneymaker. The two most aggressive populations of each species were selected and further tested on 33 tomato genotypes. The resistance screening and mechanism of resistance was performed after inoculation with 100 freshly hatched (<24 h) second-stage juveniles (J2). Eight weeks after inoculation the number of egg masses produced on each cultivar was assessed. For the resistance mechanism study, J2 penetration and their subsequent development inside the tomato roots were examined at 1, 2, 4 and 6 weeks after inoculation. On both cultivars Marmande and Moneymaker all M. incognita and M. javanica populations formed a high number of egg masses indicating highly aggressive behaviour. Populations from ‘Jittu’ and ‘Babile’ for M. incognita and ‘Jittu’ and ‘Koka’ for M. javanica were selected as most aggressive. None of the 33 tomato genotypes were immune for these M. incognita and M. javanica populations. However, several tomato genotypes were found to have a significant effect on the number of egg masses produced indicating possible resistance. For M. javanica populations there were more plants from cultivars or breeding lines on which no egg masses were found compared to M. incognita populations. The lowest number of egg masses for both populations of M. incognita was produced on cultivars Bridget40, Galilea, and Irma while for M. javanica it was on Assila, Eden, Galilea, Tisey, CLN-2366A, CLN-2366B and CLN-2366C. Tomato genotypes, time (weeks after inoculation) and their interaction were significant sources of variation for J2 penetration and their subsequent development inside the tomato roots. Differential penetration was found in breeding lines such as CLN-2366A, CLN-2366B and CLN-2366C, but many of the selected tomato genotypes resistance for the tested M. incognita and M. javanica populations were expressed by delayed nematode development. Therefore, developing a simple screening technique to be used by local farmers or extension workers is crucial to facilitate selection of a suitable cultivar. 相似文献
992.
Tania S. Peña James R. Watson Laura I. González-Guzmán Timothy H. Keitt 《Landscape Ecology》2017,32(8):1643-1656
Context
Many nearshore species are distributed in habitat patches connected only through larval dispersal. Genetic research has shown some spatial structure of such metapopulations and modeling studies have shed light onto possible patterns of connectivity and barriers. However, little is known about human impact on their spatial structure and patterns of connectivity.Objectives
We examine the effects of fishing on the spatial and temporal dynamics of metapopulations of sedentary marine species (red sea urchin and red abalone) interconnected by larval dispersal.Methods
We constructed a metapopulation model to simulate abalone and sea urchin metapopulations experiencing increasing levels of fishing mortality. We performed the modularity analysis on the yearly larval connectivity matrices produced by these simulations, and analyzed the changes of modularity and the formation of modules over time as indicators of spatial structure.Results
The analysis revealed a strong modular spatial structure for abalone and a weak spatial signature for sea urchin. In abalone, under exploitation, modularity takes step-wise drops on the path to extinction, and modules breakdown into smaller fragments followed by module and later metapopulation collapse. In contrast, sea urchin showed high modularity variation, indicating high- and low-mixing years, but an abrupt collapse of the metapopulation under strong exploitation.Conclusions
The results identify a disruption in larval connectivity and a pattern of collapse in highly modular nearshore metapopulations. These results highlight the ability of modularity to detect spatial structure in marine metapopulations, which varies among species, and to show early changes in the spatial structure of exploited metapopulations.993.
John B. Graham Joan I. Nassauer William S. Currie Herbert Ssegane M. Cristina Negri 《Landscape Ecology》2017,32(5):1023-1037
Context
Wild bee populations are currently under threat, which has led to recent efforts to increase pollinator habitat in North America. Simultaneously, U.S. federal energy policies are beginning to encourage perennial bioenergy cropping (PBC) systems, which have the potential to support native bees.Objectives
Our objective was to explore the potentially interactive effects of crop composition, total PBC area, and PBC patches in different landscape configurations.Methods
Using a spatially-explicit modeling approach, the Lonsdorf model, we simulated the impacts of three perennial bioenergy crops (PBC: willow, switchgrass, and prairie), three scenarios with different total PBC area (11.7, 23.5 and 28.8% of agricultural land converted to PBC) and two types of landscape configurations (PBC in clustered landscape patterns that represent realistic future configurations or in dispersed neutral landscape models) on a nest abundance index in an Illinois landscape.Results
Our modeling results suggest that crop composition and PBC area are particularly important for bee nest abundance, whereas landscape configuration is associated with bee nest abundance at the local scale but less so at the regional scale.Conclusions
Strategies to enhance wild bee habitat should therefore emphasize the crop composition and amount of PBC.994.
Substrate components produced in the sub-tropics or tropics were combined in various proportions as substrates for the greenhouse production of ‘Big Beef’ tomato. Coir was mixed with parboiled rice hulls (PBH) or burnt rice hulls (BRH) at 0, 25, 50, 75, or 100% volume. Fruit fresh weights at equal proportions of BRH or PBH in coir were similar, with the greatest fruit yields achieved with 50 to 100% coir (0 to 50% PBH or BRH). Compared to 100% coir, PBH in coir decreased substrate container capacity and increased air porosity, while BRH in coir increased container capacity and decreased the air porosity. Pre-plant fertilisation of the PBH + coir substrates (superphosphate, gypsum, trace elements, calcium nitrate, and surfactant) increased fruit yield significantly. Substrates containing 50 to 100% coir with BRH or PBH yielded greater fruit fresh weights than was achieved in in 70% BRH + 30% gravel (a typical substrate used in the Dominican Republic), and reusing the 70% BRH + 30% gravel led to a 36% increase in fruit fresh weight. Fruit fresh weights in 100% coir or 75% coir plus 25% PBH or BRH were similar to those achieved in a commercial peat-lite. Herein we report on substrates made from tropical components that perform similarly to a peat-based substrate in the production of greenhouse-grown tomatoes. 相似文献
995.
W. Zhang N. S. Zhang J. J. Zhao Z. Y. Zhao L. X. Mei 《The Journal of Horticultural Science and Biotechnology》2017,92(5):539-549
Potassium (K) fertilization and antioxidant enzymes both positively influence plant growth and development. However, it is not known whether K treatment improves fruit development via increasing soluble sugar. In this study, K-treated apple (Malus domestica Borkh.) fruit was harvested from 60 to 150 days after full bloom and was analyzed for ROS production and trehalose metabolism. The results show that K fertilization improved fruit firmness, increased growth according to several parameters, increased soluble sugar content, and decreased ROS production. The ascorbate metabolic pathway more effectively reduced ROS production than catalase and peroxidase (POD) did under K treatment. Trehalose-treated fruit also showed higher activity of ascorbate-related enzymes (DHAR, GR, and APX) compared with non-treated fruit. The changes of antioxidant enzyme activity in trehalose-treated fruit corresponded to those in K-treated fruit. Moreover, trehalase (TREH) activity in fruit was notably reduced by K treatment. This demonstrates that K influences ROS production via regulating trehalose content and TREH activity in fruit. This study provides new insight into the K mechanism which improves fruit development, including fruit firmness and size. 相似文献
996.
An economically based evaluation index for perennial and short‐term ryegrasses in New Zealand dairy farm systems
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D. F. Chapman J. R. Bryant M. E. Olayemi G. R. Edwards B. S. Thorrold W. H. McMillan G. A. Kerr G. Judson T. Cookson A. Moorhead M. Norriss 《Grass and Forage Science》2017,72(1):1-21
Grazed pastures based on ryegrass species provide most of the feed for dairy cattle in New Zealand. There are many cultivars of perennial (Lolium perenne), annual and Italian (L. multiflorum), and hybrid (L. boucheanum) ryegrasses available for dairy farmers to use in pasture renewal. This study describes an index which ranks ryegrass cultivars relative to a genetic base according to the estimated economic value (EV) of seasonal dry matter (DM) traits. A farm system model was used to derive EVs (Grazed pastures based on ryegrass species provide most of the feed for dairy cattle in New Zealand. There are many cultivars of perennial (Lolium perenne), annual and Italian (L. multiflorum), and hybrid (L. boucheanum) ryegrasses available for dairy farmers to use in pasture renewal. This study describes an index which ranks ryegrass cultivars relative to a genetic base according to the estimated economic value (EV) of seasonal dry matter (DM) traits. A farm system model was used to derive EVs ($ ha?1 calculated as change in operating profit divided by unit change of the trait) for additional DM produced in different seasons of the year in four regions. The EV of early spring DM was consistently high across all regions, whereas EV for late spring DM was moderate to low. Genotype × environment analysis revealed significant reranking of DM yield among ryegrass cultivars across regions. Hence, separate performance values (PVs) were calculated for two mega‐environments and then combined with the corresponding season and region EV to calculate the overall EV for twenty‐three perennial ryegrass and fifteen short‐term ryegrass cultivars. The difference in operating profit between the highest ranked and lowest ranked perennial ryegrass cultivar ranged from $556 ha?1 to $863 ha?1 year?1 depending on region. For short‐term ryegrasses used for winter feed, the corresponding range was $394 to $478 ha?1 year?1. Using PV for DM yield, it was estimated that plant improvement in perennial ryegrass has added $12–$18 ha?1 year?1 (depending on region) operating profit on dairy farms since the mid‐1960s. 相似文献
997.
Impact of sampling and storage technique,and duration of storage,on the composition of fresh grass when analysed using near‐infrared reflectance spectroscopy
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The objective of this study was to examine the effect of sampling technique (pluck or cut), storage duration (immediate analysis, 24‐h or 48‐h), storage temperature (ambient or chilled) and storage conditions (air present, air excluded or breathable) on the composition of fresh grass sampled from a sward managed to simulate grazing. Treatments were repeated across four sampling dates, with grass samples stored in grip seal bags prior to analysis using near‐infrared reflectance spectroscopy. Grass sampled by ‘pluck’ had a higher crude protein and ME content, and a lower acid detergent fibre (ADF) content, compared to that sampled by ‘cut’. Grass stored for 48 h had a lower water soluble carbohydrate (WSC) and ME content and a higher ADF content than for immediate analysis. Samples stored for 24 h did not differ from immediate analysis. Grass stored at ambient temperature had a lower WSC and ME content compared to immediate analysis. Grass stored under ‘breathable’ conditions had a lower ME content and higher ADF content than immediate analysis or samples stored with air present or air excluded. It is recommended that grass for analysis should be sampled by cutting, stored chilled (4°C) in a sealed bag to minimize exposure to oxygen and analysed within 24 h of harvest. 相似文献
998.
R. Vilcacundo D. Barrio C. Carpio A. García-Ruiz J. Rúales B. Hernández-Ledesma W. Carrillo 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(3):294-300
Quinoa protein concentrate (QPC) was extracted and digested under in vitro gastrointestinal conditions. The protein content of QPC was in the range between 52.40 and 65.01% depending on the assay used. Quinoa proteins were almost completely hydrolyzed by pepsin at pH of 1.2, 2.0, and 3.2. At high pH, only partial hydrolysis was observed. During the duodenal phase, no intact proteins were visible, indicating their susceptibility to the in vitro simulated digestive conditions. Zebrafish larvae model was used to evaluate the in vivo ability of gastrointestinal digests to inhibit lipid peroxidation. Gastric digestion at pH 1.2 showed the highest lipid peroxidation inhibition percentage (75.15%). The lipid peroxidation activity increased after the duodenal phase. The digest obtained at the end of the digestive process showed an inhibition percentage of 82.10%, comparable to that showed when using BHT as positive control (87.13%). 相似文献
999.
N. J. Grünwald G. A. Forbes W. Perez-Barrera J. E. Stewart V. J. Fieland M. M. Larsen 《Plant pathology》2019,68(3):557-565
An unknown Phytophthora species was discovered in the central Peruvian Andes on blighted foliage of the native South American plant species Urera lacineata. Urera is a genus of native flowering shrubs in the nettle family Urticaceae. This new taxon Phytophthora urerae sp. nov. is herewith formally described based on extensive morphological analysis, phylogenetic analysis of nuclear and mitochondrial loci, and AFLP analysis. Phytophthora urerae sp. nov. is a close relative of the Irish famine pathogen, Phytophthora infestans, and only the third clade 1c taxon described from South America to date. In contrast to the clade 1c taxon Phytophthora andina, first described in South America as a hybrid, P. urerae does not appear to be a hybrid based on cloning and sequencing nuclear loci. Findings of new species in South America may provide novel insights into the origin and evolutionary history of clade 1c Phytophthora species. 相似文献
1000.
D. Sadiković B. Piškur I. Barnes T. Hauptman D. Diminić M. J. Wingfield D. Jurc 《Plant pathology》2019,68(6):1120-1131
Brown spot needle blight (BSNB), a disease of pine trees caused by the fungus Lecanosticta acicola, has been known in Slovenia since 2008 and in Croatia since 1975. Recent outbreaks in Slovenia prompted this study to compare L. acicola populations in these two neighbouring European countries. Sixty-nine isolates collected from three pine species (Pinus mugo, P. halepensis and P. nigra) were used to determine the phylogenetic relationships, genetic structure, and reproductive strategy of the pathogen. EF1-α sequences showed that Slovenian and Croatian isolates share a common ancestry with individuals from central and northern Europe. Population structure analysis revealed four distinct population clusters of L. acicola in these two countries, generally corresponding to their respective geographic location and host. An unequal ratio of mating types and a low overall genetic diversity in the population indicated a strong influence of asexual reproduction. Although some of the oldest recorded European occurrences of BSNB are from Croatia, this study provided no evidence that the population studied in Croatia was the source of the sampled outbreaks in Slovenia. Recent outbreaks of L. acicola in Slovenia are most likely due to introductions from other, yet to be identified, sources. 相似文献