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Genetic analysis of resistance of plant introduction (PI) 438489B to soybean cyst nematode (SCN) have shown that this PI is
highly resistant to many SCN HG types. However, validation of the previously detected quantitative trait loci (QTL) has not
been done. In this study, 250 F2:3 progeny of a Magellan (susceptible) × PI 438489B (resistant) cross were used for primary genetic mapping to detect putative
QTL for resistance to five SCN HG types. QTL confirmation study was subsequently conducted using F6:7 recombinant inbred lines (RILs) derived from the same cross. Simple sequence repeat (SSR) and single nucleotide polymorphism
(SNP) markers were employed for molecular genotyping. Interval mapping (IM), permutation tests, cofactor selection, and composite
interval mapping (CIM) were performed to identify and map QTL. Results showed that five QTL intervals were associated with
resistance to either multiple- or single-HG types of SCN. Among these, two major QTL for resistance to multiple-SCN HG types
were mapped to chromosomes (Chr.) 8 and 18, consistent with the known rhg1 and Rhg4 locations. The other QTL were mapped to Chr. 4. The results of our study confirmed earlier reported SCN resistance QTL in
this PI. Moreover, SSR and SNP molecular markers tightly linked to these QTL can be useful for the near-isogenic lines (NILs)
development aiming to fine-mapping of these QTL regions and map-based cloning of SCN resistance candidate genes. 相似文献
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Stable integration and expression of a cry1Ia gene conferring resistance to fall armyworm and boll weevil in cotton plants 下载免费PDF全文
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A. R. Griffin Tran Duc Vuong J. L. Harbard C. Y. Wong C. Brooker R. E. Vaillancourt 《New Forests》2010,40(2):131-142
Acacia mangium is a major plantation species for the pulp and paper industry in south-east Asia and there are a number of active breeding
programs. The species is predominantly outcrossing, but with a demonstrated capacity to set selfed seed where outcross pollen
is limited, with consequent inbreeding depression in the progeny. Current controlled pollination methods therefore include
a time-consuming emasculation step. We used microsatellite genotyping of seedlings to determine the consequences of outcross
pollination with and without emasculation. Only 1 of 3 mother trees set a small amount (5%) of selfed seed. Using whole inflorescences
from the male parent as the pollen applicator rather than sieved pollen reduced outcross contamination rates from 19.1 to
8.7% and substantially increased worker productivity. Application of sugar solution to the female flowers immediately prior
to pollination increased yield of sound seeds per spike. Additional improvements to the pollination protocols are discussed. 相似文献
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Quang Toan Dinh Dongli Liang Tran Thi Anh Thu Trong Dieu Hien Le Nguyen Dinh Vuong Van Tat Pham 《Archives of Agronomy and Soil Science》2018,64(3):371-383
In the context of widespread saline and sodic soil, mapping and monitoring spatial distribution of soil salinity and sodicity are important for utilization and management in agriculture lands. In this study, two-stage assessment was proposed to predict spatial distribution of saline and sodic soils. First, artificial neural network (ANN) and multiple linear regressions (MLR) model were used to predict sodium adsorption ratio (SAR) and exchangeable sodium percentage (ESP) based on soil electrical conductivity (EC) and pH. Then, the Kriging interpolation method combined with overlay mapping technique was used to perform saline spatial predictions in the study area. The model accuracy level is evaluated based on coefficient of determination (R2) and root mean square error (RMSE). In the first stage, the values of R2 and RMSE of SAR and ESP were 0.94, 0.17 and 0.94, 0.24 for ANN, and 0.35, 0.52 and 0.34, 0.76 for MLR, respectively. Similarly, in the second stage, the RMSE of ANN-Kriging were much closer to 0 and relatively lower than MLR-Kriging and Kriging. The results show that ANN-Kriging can be used to improve the accuracy of mapping and monitoring spatial distribution of saline and sodic soil in areas that develop the rice-shrimp cultivation model. 相似文献
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Vuong Christine N. Chou Wen-Ko Berghman Luc R. 《Veterinary research communications》2019,43(2):115-122
Veterinary Research Communications - Dendritic cells (DC) are important antigen-presenting cells and are among the least characterized immune cells in the chicken. In order to obtain chicken DC,... 相似文献
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Susceptibility of freshwater rearing Asian seabass (Lates calcarifer) to pathogenic Streptococcus iniae 下载免费PDF全文
Pattanapon Kayansamruaj Ha Thanh Dong Vuong Viet Nguyen Hai Dinh Le Nopadon Pirarat Channarong Rodkhum 《Aquaculture Research》2017,48(2):711-718
Asian seabass (Lates calcarifer) has been recognized as an economically important aquaculture species which can be adapted to and cultivated in wide range of salinities. The number of freshwater intensive seabass farms in Thailand is increasing annually. Here, we first describe the susceptibility of Asian seabass, which were cultured in freshwater, to Streptococcus inae (SI) and their pathological changes. Three isolates of putative SI were identified using a combination of standard biochemical assays and species‐specific PCR prior subjected to in vivo challenge. Accumulated mortalities of the fish which received 107 CFU fish?1 of either SI1J, SI SGSA or SI2J were 90%, 90% and 100% at 7 days‐post infection (dpi), respectively, and mortalities increased sharply between 3 and 5 dpi. Clinical signs such as erratic swimming and opaque eyes were identified from a few infected fish, while most died rapidly without any abnormal signs. Histopathological manifestations were observed in the multiple organs (kidney, liver and brain). Haemorrhage, hyperhemia, cellular degeneration and inflammatory cells infiltration were commonly found within the internal organs. Notably, the formation of numerous encyst‐like lesion aggregated by eosinophilic cells, resembling macrophages, were typically found in the brain of the infected fish. Summarily, this study first revealed that freshwater reared Asian seabass is highly susceptible to SI infection and haemorrhagic septicaemia was a major pathological change that could be found in the infected fish. 相似文献