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101.
Eduardo Reséndiz Sergio Flores-Ramírez Volker Koch Amaury Cordero-Tapia 《Journal of aquatic animal health》2016,28(4):252-257
Fibropapillomatosis (FP) is characterized by multiple fibroepithelial tumors in all parts of the skin and has been reported in sea turtles worldwide. Clinically infected individuals are often emaciated and anemic. In Mexico, however, there are few records of this disease. In this study of green turtles Chelonia mydas in Laguna San Ignacio in Baja California Sur (BCS), we noted one juvenile with multifocal fibropapilloma lesions on the external upper surface of its eyes and hind flippers. Light microscopy revealed hyperkeratosis, epidermal hyperplasia, dermal papillary projections, and fibroblast proliferation. Electron microscopy revealed viral particles. Biopsies of normal skin were done to determine the origin of the turtle through genetic analysis. Its mitochondrial DNA matched that of a haplotype (CMP2) from a Hawaiian green turtle population. Finding FP in a turtle captured in BCS elucidates the need for further monitoring along the west coast of Mexico. Further investigation should include testing tumors to detect and characterize any chelonid herpesviruses and explore any association with FP and other diseases that pose a health risk to other sea turtle species.
Received March 26, 2016; accepted August 3, 2016 Published online October 27, 2016 相似文献
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Millet A Lamy E Jonas D Stintzing F Mersch-Sundermann V Merfort I 《Journal of agricultural and food chemistry》2012,60(9):2148-2156
In this study, we compared the analytical fingerprint and bioactivity of three onion extracts, including an aqueous, a methanol, and a fermented aqueous extract. The extracts were characterized by HPLC-DAD, LC-MS, and GC-MS analyses. The antibacterial, antigenotoxic, and antiproliferative activity of these extracts was assessed by means of agar disk diffusion, bacterial growth kinetics, a comet assay, cell cycle distribution analysis, and cell viability testing. Both the aqueous and methanolic extracts showed a typical flavonol-fingerprint as assessed by HPLC measurements and showed little to no bioactivity. The fermented aqueous extract, which lacks the usual onion flavonoid profile, was found to be the most active in all of the assays. This finding indicates that metabolites of onion compounds, generated by lactic acid fermentation, may be more active than their precursor substances. 相似文献
104.
猪瘟是一种高度传染性疾病,能在世界范围内引起猪群的大幅度减少.这种疾病在亚洲和中南美的大部分地区、欧洲和非洲的部分地区都有发生.澳大利亚、美国、加拿大以及欧盟等国家已经根除了这种疾病,但南非、德国、荷兰、英国等国还有零星复发.引发猪瘟的病毒属于黄病毒科瘟病毒属的一个成员.对于猪瘟的首次诊断是根据兽医对临床症状和尸体剖解后作出判定的,但许多症状也不完全与猪瘟有关,它们可能会随着病毒株的不同、猪的年龄与健康状况的差异而改变.由于临床症状可能会与其他猪传染病混淆,因此猪瘟的实验室诊断是必不可少的.国际兽疫局和欧盟已经批准了猪瘟诊断手册,为本病的确诊建立取样方法和诊断程序.本文对目前诊断方法的现状进行分析并补充一些新的发展,重点在于介绍反转录聚合酶链式反应(RT-PCR)技术. 相似文献
105.
The teat canal of lactating dairy cattle is of particular importance for the defence of facultative pathogenic and pathogenic microorganisms (e.g., staphylococci) invading the bovine udder. Furthermore the canal is usually colonized with microorganisms, too. In addition to microorganisms inhibiting mastitis pathogens the teat canal is colonized by staphylococci. The microbial colonization can be influenced by the environment of the animals, the care and disinfection of the teat skin and indirectly by the effects of forces being associated with machine milking. Because of vacuum fluctuations occurring under the teat tip microorganisms, which colonize the teat canal, can invade the bovine mammary gland and cause infections there. This paper gives a review of the microbial colonization of the bovine teat duct and of influencing factors on the microbial populations as well as of the significance of the teat canal colonization for the development of mastitis. 相似文献
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Journal of Plant Diseases and Protection - 相似文献
108.
IMAGING DIAGNOSIS – SEMINOMA CAUSING LIVER COMPRESSION IN A SPUR‐THIGHED TORTOISE (TESTUDO GRAECA) 下载免费PDF全文
Michael Pees Eberhard Ludewig Bastian Plenz Volker Schmidt 《Veterinary radiology & ultrasound》2015,56(2):E21-E24
A 13‐year‐old male spur‐thighed tortoise (Testudo graeca) was presented with anorexia, apathy, and prolapse of penile tissue. Ultrasonography revealed a large heterogeneous mass in the coelomic cavity, and fine‐needle aspiration demonstrated sperm. Magnetic resonance imaging showed a sharply defined mass originating from the left testis. Appearance and signal intensities were similar to those reported in testicular neoplasms in humans, in particular sharing similarities with seminomas. Necropsy results and histopathological findings were consistent with a seminoma. To the authors’ knowledge this is the first report of the diagnosis of testicular neoplasia in a reptile using imaging techniques. 相似文献
109.
In 2009, 26 clinical samples (organs and oral/cloacal swabs) from a total of 24 corn snakes (Pantherophis guttatus) from a single owner were sent to our laboratory to be tested for the presence of viruses. Paramyxoviruses (PMV), adenoviruses (AdV) and reoviruses were detected by RT-PCR, PCR and virus isolation methods. Three snakes were infected with all three viruses at the same time, while two other snakes had a double infection (PMV and reo, AdV and reo) and nine other snakes had a single infection with any of the three viruses. No viruses were detected in 10 animals. All isolated reoviruses were identical to one another and to the reptilian orthoreovirus isolate 55-02 in the partial RNA dependent RNA polymerase (RDRP) gene sequence. AdV partial polymerase sequences represented four different types, one of which was first described here: most similar to SnAdV-1, while the other three were identical to known types: SnAV-1, -2 and -3. However, the detected single PMV differed distinctly from described reptile PMV and was a new type. According to partial L gene, HN gene and U gene sequences it may be the first described representative of a third squamatid PMV cluster: "group C" within the proposed reptilian PMV genus "Ferlavirus". Nucleotide identity values for the L gene of the new PMV compared to group A viruses range between 76.5 and 80.3%, and between 80.5 and 81.2% compared to group B viruses. For the HN gene, these values were similar: 78.2-80% (A) and 79.9-80.5% (B) and somewhat lower for the U gene: 72.7-75.4% (A) and 69.7-70% (B). No reports on the prevalence of concurrent viral infection in captive snake populations have been published so far. The possibility of concurrent infection with several different viruses and subsequent consequences for animal health should be kept in mind when testing reptile samples for viruses. 相似文献
110.
Charlotte E. Gonzalez-Abraham Volker C. Radeloff Roger B. Hammer Todd J. Hawbaker Susan I. Stewart Murray K. Clayton 《Landscape Ecology》2007,22(2):217-230
Housing growth is prevalent in rural areas in the United States and landscape fragmentation is one of its many effects. Since
the 1930s, rural sprawl has been increasing in areas rich in recreational amenities. The question is how housing growth has
affected landscape fragmentation. We thus tested three hypotheses relating land cover and land ownership to density and spatial
pattern of buildings, and examined whether building density or spatial pattern of buildings was a better predictor for landscape
fragmentation. Housing locations were mapped from 117 1:24,000-scale USGS topographic maps across northern Wisconsin. Patch-level
landscape metrics were calculated on the terrestrial area remaining after applying 50, 100 and 250 m disturbance zones around
each building. Our results showed that building density and the spatial pattern of buildings were affected mostly by lake
area, public land ownership, and the abundance of coniferous forest, agricultural land, and grassland. A full 40% of the houses
were within 100 m of lakeshores. The clustering of buildings within 100 m of lakeshores limited fragmentation farther away.
In contrast, agricultural and grassland areas were correlated with higher building density, higher fragmentation, and more
dispersed building pattern possible legacies of agricultural settlement patterns. Understanding which factors influence building
density and fragmentation is useful for landscape level planning and ecosystem management in northern Wisconsin and areas
that share similar social and environmental constraints. 相似文献