<Emphasis Type="Italic">Still</Emphasis> a time to act: A review of institutional marketing of regionally-grown food

Regional institutional marketing supports sustainable farming by bringing wholesome, nutritious food to members of the community. Schools, in particular, can benefit greatly from this arrangement in comprehensive efforts to address childhood obesity. Nineteen previous publications examined issues around supply of and/or demand for regional food procurement by institutions across the United States, including levels of interest, perceived benefits, and barriers to this arrangement. Food service directors, farmers, and/or distributors participated in surveys, interviews, workshops/forums, case studies, and one evaluation about regional food procurement. Accounts of seven farmer cooperatives or networks indicate that institutional customers are more often restaurants (n = 5), health care facilities (n = 2), colleges/universities (n = 2), and other facilities (n = 2), than public schools (n = 1) or food retailers (n = 1). The studies agree that the main benefits offered by regional food procurement are support of the local economy and increased access to fresh and nutritious food. Barriers consistently faced by food services and farmers have to do with lack of infrastructure and financial support for processing and central distribution. Though obstacles vary by district and/or geographic characteristics, results indicate that across groups there is a clear need for better support mechanisms by which farms can connect with regional markets. The practice of farm-to-institution marketing holds the potential to improve nutritional status of community members and financial stability of farmers, though institutional support is needed for systemic transition to this purchasing method.

Uber das Vorkommen von Calciumoxalat-Kristallen1 in Heterobasidion annosum (Fr.) Bref.

The increasing appearance of rods (»Stäbchen«) being found in Heterobasidion annosum cultures was investigated. They proved to be neither bacterial impurities nor fomannosin but calcium oxalates. The number of rod-shaped crystals depended on the quality of the tap water taken for the culture medium and on genetical aspects. The importance of calciumoxalates being formed by H. annosum is discussed.     

Variation among New Zealand isolates of Sphaeropsis sapinea

Sphaeropsis sapinea is a pathogen of many coniferous species and causes significant losses to the plantation forestry industries of many countries. New Zealand isolates of S. sapinea were examined for colony and morphological characteristics, and pathogenicity to Pinus radiata seedlings. Considerable variation was observed in colony growth form and colour, and in the growth rates on four agar media. The conidial dimensions, carbon and nitrogen utilization, and pathogenicity also varied among the isolates. The variation observed was such that the isolates could not be placed within previously described distinct morphotypes and the present results concur with previous studies cautioning the use of morphological criteria alone to partition the species. These results contrast markedly with previous reports and seemingly indicate a change in the New Zealand S. sapinea population. This difference may reflect the re‐introduction or spread of additional isolates or a shift in the structure of the resident population.     

Application of high-resolution melt curve analysis for classification of infectious bronchitis viruses in field specimens

Objective A real-time polymerase chain reaction (PCR)/high-resolution melt (HRM) curve analysis protocol was developed in our laboratory to differentiate infectious bronchitis (IB) virus reference strains. In the current study, this method was used to detect and classify IB viruses in field submissions. Procedure Over an 11-month period samples from 40 cases of suspected IB virus were received and 17 submissions were positive for IB virus by polymerase chain reaction. HRM curve analysis classified each strain as subgroup 1, 2 or 3 strain (12 submissions) or a strain that was unable to be classified (5 submissions). The 3′ untranslated region (UTR) and partial S1 gene nucleotide sequences for the 17 IB virus strains were determined and their identity with those of the relative reference strains compared to confirm the classifications generated using the HRM curve analysis. Results Of the 12 IB field viruses classified as subgroup 1, 2, or 3 using HRM curve analysis, the 3′UTR and S1 gene nucleotide sequences had identities ≥99% with the respective subgroup reference strain. Analysis of the 3′ UTR and S1 gene nucleotide sequences for the five IB virus strains that could not be classified indicated that four belonged to one of the subgroups, and one was a potential recombinant strain (between strains from subgroups 2 and 3). A novel recombinant strain was also detected. Conclusion HRM curve analysis can rapidly assign the majority of IB viruses present in field submissions to known subgroups. Importantly, HRM curve analysis also identified variant genotypes that require further investigation.     

The effect of 3‐nitro‐4‐hydroxyphenylarsonic acid (3‐nitro‐hoechst) on egg production and food conversion of laying hens and residue analyses of muscle,organs and eggs

White Leghorn pullets were divided into two groups, each comprising 5 pens with 15 or 16 birds per pen. The experiment began when the birds were 20 weeks old and finished on the 500th day of life. The control group received a normal ration containing 16 per cent protein whilst the experimental group received the same ration supplemented with 50 p.p.m. 3‐nitro‐4‐hydroxyphenylarsonic acid (3‐nitro‐hoechst).

Food consumption and egg weight were similar in both groups but the treated group laid 4 per cent more eggs. Although this was not significant, the treated group showed an overall improvement in the efficiency of food utilisation of 2.5 per cent.

For a period of 6 months the arsenic content of 10 eggs was determined weekly and after this time at fortnightly intervals. At the end of the experiment 10 birds of each group were killed and the arsenic contents of the heart, liver, kidneys, tibia, pectoral muscle and feathers were determined. The arsenic contents of the eggs, heart, kidneys, muscle and bone of the experimental birds were not significantly different from the controls. However, the liver of the birds fed the supplement contained twice and the feathers fifteen times as much arsenic as the control birds.     

Einfluss der Zwischenlagerung auf die anfängliche Entwicklung rekultivierter Oberböden

Influence of temporary stockpiling on the initial development of restored topsoils Due to increasing construction and open‐cast mining activities on fertile agricultural land, excavation, stockpiling, and restoration of soils have become important issues in soil protection. In this study, we performed a restoration experiment to investigate how the conditions during stockpiling may influence initial soil development and plant growth on a restored site. Four topsoils, which originated from two depths (with uninhibited and inhibited aeration) of a nontrafficked and a trafficked topsoil deposit, were repacked in strips and sown with three meadow‐seed mixtures in strips perpendicular to the soil strips. During stockpiling as well as during the first 2 years after restoration, we assessed the physical and mechanical properties of the topsoils and (after restoration) also plant growth. None of the investigated properties of the topsoils was influenced by the depth in the deposit. In contrast, the coarse porosity and compression index were lower, whereas the bulk density and precompression stress were higher in the trafficked than in the nontrafficked topsoil deposit. However, these differences largely disappeared already in the course of soil restoration. Contrary to our expectations, the plants grew better on the soil of the trafficked topsoil deposit than on the soil of the nontrafficked topsoil deposit in the year of restoration. This might be attributed to the extremely dry and hot summer of that year. In the course of the 2 years following restoration, also the differences in plant growth tended to disappear. The remaining differences in bulk density and plant growth could not be attributed to differences in stockpiling conditions. The results suggest a re‐examination of current soil restoration guidelines. The maximum permitted stockpiling heights for topsoils might be increased in order to reduce the areas required for temporary stockpiling.     

Composting of Yard Waste and Wastewater Treatment Plant Sludge Mixtures

Composting of yard waste and sludge/yard waste mixtures was investigated during laboratory and field testing. A strong correlation between moisture content and windrow peak temperatures was observed. Odors were detected at cocomposting windrows when temperatures exceeded 54°C. The sludge to yard waste mixture ratio was found to affect resulting compost particle size and, consequently, oxygen transport. Compost quality was excellent, with pesticides below detection level and low concentrations of heavy metals.     

Improvement in In Vitro Fertilization Rate,Decrease in Reactive Oxygen Species and Spermatozoa Death Incidence in Rams by Dietary Fish Oil

Our aim was to evaluate the effects of fish oil feeding on sperm classical parameters, level of reactive oxygen spices (ROS), spermatozoa death incidence and in vitro fertilization (IVF) rate in rams. We randomly assigned nine rams, into two experimental groups (isoenergetic and isonitrogenous rations with constant level of vitamin E supplement): control (CTR; n = 5) and fish oil (FO; n = 4, 35 g/day/ram). Diets were fed for 70 days during the physiological breeding season. After a 21‐day dietary adaptation period, semen was collected weekly from each ram by an artificial vagina. Sperm classical parameters were determined by the computer‐assisted sperm analyzer system (CASA), and it was prepared for IVF process by swim‐up technique. These evaluations were performed during the first and last weeks of sampling. Intracellular ROS level and spermatozoa death incidence were detected by flow cytometry on a weekly basis after adaptation. Data were analysed with SPSS 15. The volume, concentration (3.6 and 2.7 × 10⁹/ml) and sperm progressive motility (60 and 48%) were significantly improved in the FO group compared with the CTR (p < 0.05). A comparison of two‐cell stage embryos following IVF in the two groups showed a significantly higher fertilization rate in the FO group (56%) compared with the CTR (49%). Superoxide anion (O₂^?) rate was significantly lower (p < 0.05) at the third week of sampling in the FO. Although the H₂O₂ rate was numerically lower in the FO group compared with the CTR, this difference was not significant. In addition, apoptosis showed a significant difference in the third week of sampling (15 and 30% for FO and CTR, respectively; p < 0.05). Overall, adding fish oil to the ram diet not only improved sperm quality and IVF results, it also could reduce oxygen‐free radicals and the incidence of spermatozoa death.