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131.
132.
Anna Carbone Barbara Parrino Paola Barraja Virginia Spanò Girolamo Cirrincione Patrizia Diana Armin Maier Gerhard Kelter Heinz-Herbert Fiebig 《Marine drugs》2013,11(3):643-654
2,5-bis(3′-Indolyl)pyrroles, analogues of the marine alkaloid nortopsentin, were conveniently prepared through a three step procedure in good overall yields. Derivatives 1a and 1b exhibited concentration-dependent antitumor activity towards a panel of 42 human tumor cell lines with mean IC50 values of 1.54 μM and 0.67 μM, respectively. Investigating human tumor xenografts in an ex-vivo clonogenic assay revealed selective antitumor activity, whereas sensitive tumor models were scattered among various tumor histotypes. 相似文献
133.
Belén Martínez‐Alcántara Ana Quiñones Francisco Legaz Eduardo Primo‐Millo 《植物养料与土壤学杂志》2012,175(2):282-292
The aim of this study was to improve the fertilizer‐nitrogen‐use efficiency (NUE) for a sustainable citrus production where yield, fruit quality, and environment are reconciled. A soil pot experiment was conducted using 5‐year‐old orange trees (Citrus sinensis cv. Navel Lane Late) to determine the response of NUE to timing of N‐fertilizer supply. An equal N rate (50 g tree–1) following two seasonal supply distributions was tested: (1) ENS (early supply from March to June of 75% of the N rate, with the remaining 25% administered between July and October) and (2) LNS (late supply of the bulk of the N rate with 25% between March and June and 75% between July and October). Labeled fertilizer (5 atom% 15N excess) was applied in order to accurately quantify N uptake, its partitioning among plant–soil compartments and NUE at the end of fruit drop and at fruit maturity. LNS resulted in a significant increase in NUE in both stages (up to 19% at the end of fruit drop and 9% at fruit maturity), while also increasing summer/autumn flush development. ENS showed lower fruit abscission, an enhanced final fruit load (up to 45%), and a more profuse development of spring flush. It is worth mentioning that LNS led to higher 15N content in the majority of the tree storage organs available for next spring growth resumption. The amount of 15N remaining in the soil of ENS trees at the end of the trial, which represented 16% of the supplied 15N, was 1.5‐fold higher than that of LNS trees as a result of the lower NUE of the former. Irrespective of the seasonal distribution of the fertilizer, mean 15N recovery in the soil–plant system at the end of the trial was about 71%. The results clearly confirm that the N seasonal distribution curve affects NUE in young citrus trees and NUE increases when the bulk of supply of the N rate is delayed to the summer months. 相似文献
134.
Bezos J Álvarez J Mínguez O Marqués S Martín O Vigo V Pieltain C Romero B Rodríguez S Casal C Mateos A Domínguez L de Juan L 《Research in veterinary science》2012,93(2):636-640
The aim of this study was to evaluate the specificity of the most widely used tuberculosis (TB) diagnostic tests, single intradermal tuberculin (SIT) and single comparative intradermal tuberculin (SCIT) tests and interferon-gamma (IFN-γ) assay in 937 animals from eight TB-free caprine flocks under different epidemiological situations. Maximum specificity was found using SCIT test (99.4-100% depending on the interpretation criteria) while SIT test and IFN-γ assay showed a slightly lower overall specificity (97.6-99.2% and 96.4-98.4% respectively). Specificity of the SIT test in a Corynebacterium pseudotuberculosis infected flock was significantly (P<0.05) lower if a severe interpretation criterion was applied. Similarly, specificity values of SIT test and particularly IFN-γ assay in a paratuberculosis (PTB)-vaccinated flock were lower than those observed in non-vaccinated flocks. Higher proportion of false positive reactors to TB tests (SIT and IFN-γ assay) were observed among animals positive in the PTB-ELISA in PTB vaccinated flock. These results demonstrate that TB diagnostic tests show an adequate specificity when performed in goats from TB-free flocks in most situations. However, certain factors such as C. pseudotuberculosis infection and paratuberculosis vaccination can have a negative impact in the most sensitive tests. 相似文献
135.
W. Kent Scarratt Virginia A. Buechner-Maxwell Stacey Karzenski Mark A. Wallace John L. Robertson 《Journal of Equine Veterinary Science》1999,19(10):642
Urinary incontinence, weakness and ataxia associated with equine protozoal myeloencephalitis (EPM) was diagnosed in three horses. Rectal examination of all horses revealed distention of the urinary bladder. Urine was expressed when manual pressure was applied to the bladder of each horse during rectal examination. The anal reflex and tone of the anus and tail were normal in all horses. Two horses had bacterial cystitis associated with Enterococcus sp. All horses were treated with pyrimethamine and a sulfonamide for EPM, but there was a variable response to treatment. 相似文献
136.
Virginia Hernández-Santana José Martínez-Fernández Carlos Morán Ana Cano 《European Journal of Forest Research》2008,127(5):369-378
In the present study, carried out from 2004 to 2006, leaf and stem water potential and stem water content were measured in
Quercus pyrenaica Willd. individuals in an experimental forested catchment located in Central Western Spain under Mediterranean subhumid conditions.
These indicators of tree water status were compared with soil moisture contents measured in the same area from 0 to 1 m depth
and from 0 to 2.5 m depth during the last year of the study. The objectives were to clarify the seasonal and year-to-year
variations in tree water status, to examine applicability of stem water content as useful water stress indicator and to discuss
how deep soil water and root uptake contribute to survival during the long dry summer. Seasonal variations in the tree variables
measured revealed a typical pattern, with maximum values at the end of spring followed by a progressive decline during the
summer drought in response to the decrease in soil water content (almost exhausted at 0–100 cm depth). The relatively high
values and the non-significant variation in predawn leaf water potentials (except for 2005, which was exceptionally dry) indicate
that no clear water stress situations occurred. This may be explained in terms of a progressive absorption of water from the
deeper layers. The results also suggest that the stem water content is a more sensitive indicator of long-term water limitation
than the other variables measured. 相似文献
137.
138.
The compensation heat pulse (CHP) method is widely used to estimate sap flow and transpiration in conducting organs of woody plants. Previous studies have reported a natural azimuthal variability in sap flow, which could have practical implications in locating the CHP probes and integrating their output. Sap flow of several olive trees (Olea europaea L. cv. 'Arbequina') previously grown under different irrigation treatments were monitored by the CHP method, and their xylem anatomical characteristics were analyzed from wood samples taken at the same location in which the probes were installed. A significant azimuthal variability in the sap flow was found in a well-irrigated olive tree monitored by eight CHP probes. The azimuthal variability was well related to crown architecture, but poorly to azimuthal differences in the xylem anatomical characteristics. Well-irrigated and deficit-irrigated olive trees showed similar xylem anatomical characteristics, but they differed in xylem growth and in the ratio of nocturnal-to-diurnal sap flow (N/D index). The results of this work indicate that transpiration cannot be accurately estimated by the CHP method in olive trees if a small number of sensors are employed and that the N/D index could be used as a sensitive water status indicator. 相似文献
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140.