Interference by amino acids during the determination of N ammonium in soil

In the study of terrestrial N cycling, NH₄⁺ concentration and ¹⁵N enrichment are routinely determined by colorimetric continuous flow analysis and microdiffusion methods. Amino acids can interfere in these determinations; consequently the aim of the present study was to evaluate the significance of the interference. Glycine and glutamine are key amino acids in soil and were therefore used as ‘models’. Both glycine and glutamine interfered during continuous flow analysis, whereas interference during microdiffusion was of little importance. The effects of interference can be significant, e.g. estimates of gross mineralisation rate were reduced up to 33%, where we allowed for amino acid interference during determination of NH₄⁺ concentration. The potential influence of amino acid interference emphasises that development of continuous flow analysis to increase NH₄⁺ specificity is needed.     

Implications of climate change for tillage practice in Australia

The world is experiencing climate change that in no way can be considered normal, and the challenge that this brings to agriculture is twofold. The first challenge relates to the continuing need to reduce greenhouse gas emissions that generate the changes to climate. Australia's National Greenhouse Gas Inventory estimates that agriculture produces about one-quarter of Australia's total greenhouse gas emissions (including land clearing). The main gases emitted are carbon dioxide, methane, and nitrous oxide. These gases are derived from high-value components within the agricultural production base, so reducing emissions of greenhouse gases from agriculture has the potential to provide production and financial benefits, as well as greenhouse gas reduction. Methane essentially derives from enteric fermentation in ruminants. Nitrous oxide and carbon dioxide, on the other hand, are strongly influenced, and perhaps even determined by a range of variable soil-based parameters, of which the main ones are moisture, aerobiosis, temperature, amount and form of carbon, organic and inorganic nitrogen, pH, and cation exchange capacity. Tillage has the potential to influence most of these parameters, and hence may be one of the most effective mechanisms to influence rates of emissions of greenhouse gases from Australian agriculture. There have been substantial changes in tillage practice in Australia over the past few decades – with moves away from aggressive tillage techniques to a fewer number of passes using conservative practices. The implications of these changes in tillage for reducing emissions of greenhouse gases from Australian agriculture are discussed.

The second challenge of climate change for Australian agriculture relates to the impacts of climate change on production, and the capacity of agriculture to adapt where it is most vulnerable. Already agriculture is exposed to climate change, and this exposure will be accentuated over the coming decades. The most recent projections for Australia provided by the CSIRO through the Australian Climate Change Science Programme, indicate that southern Australia can expect a trend to drying due to increased temperatures, reduced rainfalls, and increased evaporative potentials. Extremes in weather events are likely also to become more common. We anticipate that climate change will become an additional driver for continued change in tillage practice across Australia, as land managers respond to the impacts of climate change on their production base, and governments undertake a range of activities to address both emissions reduction and the impacts of climate change in agriculture and land management.     

Mars north polar deposits: stratigraphy, age, and geodynamical response

The Shallow Radar (SHARAD) on the Mars Reconnaissance Orbiter has imaged the internal stratigraphy of the north polar layered deposits of Mars. Radar reflections within the deposits reveal a laterally continuous deposition of layers, which typically consist of four packets of finely spaced reflectors separated by homogeneous interpacket regions of nearly pure ice. The packet/interpacket structure can be explained by approximately million-year periodicities in Mars' obliquity or orbital eccentricity. The observed approximately 100-meter maximum deflection of the underlying substrate in response to the ice load implies that the present-day thickness of an equilibrium elastic lithosphere is greater than 300 kilometers. Alternatively, the response to the load may be in a transient state controlled by mantle viscosity. Both scenarios probably require that Mars has a subchondritic abundance of heat-producing elements.     

Characterization of a novel coronavirus associated with severe acute respiratory syndrome

In March 2003, a novel coronavirus (SARS-CoV) was discovered in association with cases of severe acute respiratory syndrome (SARS). The sequence of the complete genome of SARS-CoV was determined, and the initial characterization of the viral genome is presented in this report. The genome of SARS-CoV is 29,727 nucleotides in length and has 11 open reading frames, and its genome organization is similar to that of other coronaviruses. Phylogenetic analyses and sequence comparisons showed that SARS-CoV is not closely related to any of the previously characterized coronaviruses.     

Insight to the genetic diversity of pigeon pea Cajanus cajan (L.) Millsp. and cowpea Vigna unguiculata (L.) Walp. germplasm cultivated in Nigeria based on rbcl gene region

Genetic Resources and Crop Evolution - Pigeon pea Cajanus cajan (L.) Millsp. and cowpea Vigna unguiculata (L.) Walp. are two of the important seed legumes of Africa and are relatively more...     

Resistant starch in cereals: Exploiting genetic engineering and genetic variation

Some of the starch consumed by humans is not digested in the small intestine. Such starch, known as resistant starch, is fermented in the large intestine and leads to the production of short chain fatty acids. Increased consumption of resistant starch is associated with improved cardio-vascular health. A high proportion of amylose in the starch consumed is correlated with increased resistant starch but other unknown aspects of starch structure may also influence the digestibility of starch. Detailed investigation of the starch biosynthetic pathway has revealed that reducing the activity of specific isoforms of branching enzymes and starch synthases can lead to increased amylose. Methods to alter the expression of and detect mutations in targeted genes involved are discussed.