Pantoea agglomerans pvs. gypsophilae and betae are related gall-forming bacteria. While P. agglomerans pv. beta initiates gall formation on both beet and gypsophila, the gypsophila pathovar causes gall formation only on gypsophila. PthG
is a type III effector determining host range of these pathogens, initiating the hypersensitivity response in beet, but is
a virulence factor in gypsophila. The role of PthG and its mode of action in pathogenicity remain unclear. Transgenic Nicotiana tabacum plants expressing pthG were created. PthG over-expression was often lethal, and surviving pthG-bearing lines showed morphological and developmental abnormalities such as leaf deformation and abnormal vascular branching,
dwarf stature, loss of apical dominance, seedling apical meristem loss, rapid germination, reduced fertility, plants which
cease growth for several weeks later producing a new lateral shoot, and loss of endophyte resistance (bearing unusual saprophyte
populations). Some transformants required light for seed germination and showed rapid seedling greening. In in vitro assays
PthG expression modified responses to auxin and cytokinin, inhibiting root and shoot production but not callus formation.
In vitro differentiation responses to light were modified by PthG expression. This effector may interfere in the plant auxin
signalling pathways resulting in higher observed auxin and ethylene levels, and subsequent blockage of root and shoot development.
Apparently PthG tunes the host response to high hormone levels, changing the developmental response. Since shoot and root
development are delayed, we hypothesize that callus/gall formation is supported by this activity. However, interference by
PthG with hormone and light signalling does not explain all the responses observed in pthG-bearing lines. 相似文献
Nothofagus dombeyi is distributed on very diverse sites in the temperate forests of Chile and Argentina. In Chile, studies on its dynamics and productivity in forests, where it is the dominant species, have been carried out mainly in the foothills of the Andes, but the productivity and growth of these forests in the Coastal Range are almost unknown. The objective of this study was to evaluate the construction of a single dominant height/age growth model for two homogeneous edaphoclimatic zones (ZEH 2 and 5) previously defined for this species in the Coastal Range of southcentral Chile. The results showed that the Chapman-Richards model was the best candidate function, in terms of goodness of fit and predictive capabilities. Using this function, specific parameters (a, b and c) were obtained for each zone, from which site models were constructed by the Algebraic Difference Approach method for a base age of 35 years. This model is intended to contribute to better estimations of site productivity for N. dombeyi forests in the Coastal Range of south-central Chile, and thus facilitate private and public decisions regarding their management. 相似文献
Fats, oils, and grease (FOG) and source separated organics (SSO) were treated with the microwave-enhanced advanced oxidation process (MW-AOP) at 90 and 110 °C, with varying amounts of hydrogen peroxide dosages. The treatment efficiency, in terms of soluble substrates and volatile fatty acids (VFA), increased with an increase in both temperature hydrogen peroxide dosages. Fatty acids and compounds with carbonyl group and/or hydroxyl group in both initial and treated FOG samples were identified by gas chromatography-mass spectrometry. MW-AOP treatment temperatures and hydrogen peroxide dosages dictated the formation of degradation products. The degradation followed peroxidation mechanism to produce lower molecular weight substrates such as short chain fatty acids which would be less inhibitory to microbes. After the MW-AOP treatment, both SSO and FOG comprised of more soluble and low molecular weight compounds. These compounds included VFA and nutrients that would be readily available for bacterial or plant uptake. 相似文献
This study assessed the usage of mobile phones among farmers in the Keana Local Government Area of Nasarawa State, Nigeria. One hundred and fifty-eight respondents were selected using purposive and random sampling techniques. Primary data were used, and collected using a structured questionnaire. Research revealed that a majority of the respondents (60.1%) had a medium level of use of mobile phones. The mobile phone network preferred by respondents was MTN (32.3%). The major factor affecting the use of mobile phones is poor network coverage (22.1%). Possession of formal education, income, and sex of the respondents significantly affected their level of use of mobile phones. It is therefore recommended that network providers be supervised by the Government to ensure quality service delivery, and other network providers encouraged to improve on their services. 相似文献
A standard area diagram set (SADs) with eight severity values ranging from 0.5 to 32% was evaluated as a tool to improve the accuracy of the estimates of Phomopsis leaf blight severity on eggplant by ten inexperienced raters. A first assessment in a 50-leaf dataset of digital images was performed unaided (UN, no SAD). Two further aided (A) assessments, conducted two (A1) and four weeks after the first (A2) unaided one were performed. The precision of the estimates, as indicated by the Pearson’s correlation coefficient (r), improved significantly when using the aid (A1: r = 0.96 and A2: r = 0.96) compared to unaided (UN: r = 0.79). However, the generalized bias (Cb) was not significantly affected because the unaided estimates were already quite accurate (Cb = 0.93). The overall concordance (ρc) was significantly improved due to the large gains in precision when using the SADs. The raters’ estimates were more uniform (ρc > 0.9) when using the aid, with progressive gains in the reliability of the estimates among them, indicated by the concordance correlation coefficient statistics. The SADs proposed in this study will be useful in severity estimation during field work involving multiple raters, especially as use of the SADs results in less variable estimates, improving accuracy and reliability among raters. 相似文献
The cover image, by Oscar Fernando Santos‐Amaya et al., is based on the Research Article Fitness costs and stability of Cry1Fa resistance in Brazilian populations of Spodoptera frugiperda, DOI: 10.1002/ps.4312 . Photo Credit: Dr. Oscar Santos‐Amaya.
Understanding the impacts of agricultural land use is desired for proper management of riparian forests buffers (RFs) in savannas. In this light, we analyzed the relationship between woody plant diversity and soil properties of RFs in farmlands (FAs) and protected areas (PAs) along Afram (Guinean zone) and Tankwidi (Sudanian zone) Rivers located within the Volta sub-basin of Ghana. ALOS-AVNIR imageries were used to map RFs to facilitate sampling of soil and woody vegetation (dbh ≥5?cm) using stratified randomized design (72 plots; size: 500?m2 each) for the analysis of soil physical and chemical properties and Shannon-Wiener plant species diversity. Although the fractions of sand, silt, and clay varied between the PAs and FAs, they were predominantly categorized as sandy loam. Along the Afram River, there were no significant differences in the concentrations and nutrient stocks of soil C, N, P, and K as well as the pH, moisture content, and bulk density between PA and FA. For the Tankwidi River, the concentrations and nutrient stocks of C and K significantly decreased from PA to FA while N, P, pH, bulk density, and moisture content were not different. Of the soil attributes measured C, N, P, pH, ground slope, and soil moisture had significant relationships with the diversity and density of the riparian woody plants. Controlling agricultural activities in riparian forests could preserve soil properties similar to that found in protected areas. 相似文献
Tree growth variables and more complex derived variables such as vigor index have all been linked to the ability of lodgepole pine (Pinus contorta var latifolia) trees to defend against insect herbivores, particularly mountain pine beetle (Dendroctonus ponderosae Hopkins, Coleoptera: Curculionidae). These variables are considered indirect measures of carbohydrate reserves. Trees with high vigor index values have high growth rates and are assumed to have high carbohydrate reserves. However, this critical assumption was untested for lodgepole pine. In this paper we evaluated the relationship between carbohydrate concentration and tree characteristics. We measured concentrations of root starch in 10 pure lodgepole pine stands of different ages in the Canadian Rockies which had been fertilized, thinned, fertilized and thinned or left untreated in a 2 × 2 factorial design. Both fertilization and thinning increased basal area increment while only fertilization increased lateral branch growth in the crown. Contrary to expectations, fertilization decreased root starch reserves although it increased basal area increment; thinning had no effect on root carbohydrates. Root starch reserves were positively related to basal area increment and vigor index in both fertilized and unfertilized plots, however, the best predictors of starch reserves were height-to-live-crown and cambial surface area below the live crown; starch reserves declined as both of these variables increased. These data suggest that large carbon sinks associated with long boles below the crown, and rapid growth of the crown and stem as a result of fertilization are detrimental to building starch reserves in the roots. 相似文献
Variable retention harvesting (VRH) has been proposed as a silvicultural practice to maintain biodiversity and ecosystem integrity. No previous study has examined tree carbon isotope discrimination to provide insights into water stress that could lead to dieback and mortality of trees following VRH. We measured and compared the carbon isotope ratios (delta(13)C) in stem wood of trembling aspen (Populus tremuloides Michx.) before and after VRH. Eight trees were sampled from isolated residual, edge and control (interior of unharvested stand) positions from each of seven plots in three regions (Calling Lake and Drayton Valley, Alberta and Lac Duparquet, Québec). After VRH, the general trend in mean delta(13)C was residual > edge > control trees. Although this trend is indicative of water stress in residual trees, it also suggests that edge trees received some sheltering effect, reducing their stress compared with that of residuals. A strong inverse relationship was found between the delta(13)C values and the mean annual precipitation in each region. The trend in mean delta(13)C signature was Calling Lake > Drayton Valley > Lac Duparquet trees. These results suggest that residual or edge trees in drier regions are more likely to suffer water stress following VRH. We also observed a trend of greater delta(13)C in stout trees compared with slender trees, both before and after VRH. The evidence of greater water stress in stout trees likely occurred because of a positive relationship between stem diameter and crown volume per basal area. Our results provide evidence that water stress could be the driving mechanism leading to dieback and mortality of residual trees shortly after VRH. Additionally, the results from edge trees indicate that leaving hardwood residuals in larger patches or more sheltered landscape positions could reduce the water stress to which these trees are subjected, thereby reducing dieback and mortality. 相似文献