首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   708篇
  免费   22篇
  国内免费   1篇
林业   77篇
农学   13篇
基础科学   5篇
  162篇
综合类   69篇
农作物   36篇
水产渔业   75篇
畜牧兽医   235篇
园艺   12篇
植物保护   47篇
  2023年   8篇
  2022年   7篇
  2021年   16篇
  2020年   22篇
  2019年   23篇
  2018年   26篇
  2017年   22篇
  2016年   13篇
  2015年   17篇
  2014年   23篇
  2013年   34篇
  2012年   32篇
  2011年   62篇
  2010年   28篇
  2009年   31篇
  2008年   52篇
  2007年   46篇
  2006年   39篇
  2005年   30篇
  2004年   30篇
  2003年   24篇
  2002年   33篇
  2001年   17篇
  2000年   10篇
  1999年   15篇
  1998年   3篇
  1997年   3篇
  1995年   5篇
  1994年   4篇
  1992年   4篇
  1991年   2篇
  1990年   4篇
  1988年   7篇
  1987年   3篇
  1986年   4篇
  1985年   2篇
  1984年   6篇
  1983年   3篇
  1981年   2篇
  1980年   2篇
  1979年   3篇
  1977年   1篇
  1975年   1篇
  1972年   1篇
  1969年   1篇
  1968年   1篇
  1961年   1篇
  1959年   1篇
  1951年   1篇
  1931年   1篇
排序方式: 共有731条查询结果,搜索用时 31 毫秒
11.
Intra‐ and interspecific characteristics of fish‐pathogenic Edwardsiella ictaluri, and E. tarda were determined by numerical analysis of gel electrophoresed protein profiles, fatty acid methyl esters (FAMEs) and immunoblotting. The 18 E. ictaluri isolates revealed a high degree of homogeneity (70% similarity or higher) in their protein profiles and 95% similarity in their FAME, while the nine E. tarda isolates revealed 30% similarity in their protein profiles and 95% similarity in their FAME. Immunoblots probed for antigenic epitopes with goat antiserum produced against E. ictaluri and E. tarda, respectively, revealed that E. ictaluri were more homogeneous compared with the E. tarda isolates. Overall, there was a considerable degree of relatedness between the two species. Our findings suggest that phenotypically E. ictaluri represents a clonal bacterial population structure compared with the less monomorphic E. tarda.  相似文献   
12.
Possibilities for disinfection of the developmental stages from the housefly (Musca domestica) were investigated under laboratory conditions. The developmental stages (eggs, larvae I, II, III, pupae and adults) were sprayed with solutions of disinfectants on the basis of p-chlorine-m-cresol and o-phenylphenol at different concentrations (0.025%-3%). The effectiveness of both disinfectants was established by determining the emergence rate of eggs and pupae and of the live evidence of larvae and adults. Transmission electron microscopic investigations of the cover of the eggs revealed the bactericidal effect of both disinfectants. These results show the necessity of extending its use also to noxious arthropods.  相似文献   
13.
Ten dogs suffered acute renal failure after ingesting > or = 3 g/kg (dry matter) of grapes or raisins. All dogs had degeneration or necrosis (or both) of proximal renal tubules with basement membranes remaining intact, and epithelial regeneration was observed in 5 out of 10 cases. Mineralized tubular debris or granular to proteinaceous casts (or both) were present in all cases. A golden-brown, globular, intracellular pigment of varying amounts and sizes was observed in 6 out of 10 cases with variable reaction with Prussian blue. Multifocal fibrinous arteritis of the large colon was seen in 2 out of 5 cases with globulin insudation of vessel wall demonstrated by immunohistochemical staining for immunoglobulin (Ig)G and IgM. Mineral analysis on frozen renal tissue from 2 out of 2 cases revealed mildly elevated Ca:P ratio in both. Clinically significant observations were preservation of the integrity of basement membranes after grape-induced tubular injury and presence of early epithelial regeneration. Thus, recovery may be possible if anuria is aggressively managed. With respect to potential pathophysiologic mechanisms, further research into the roles of calcium homeostasis, vascular reactivity, and the significance of the golden-brown pigment is indicated.  相似文献   
14.
This study examines milk production and the effect of milk production and sex of calf on body weights and gains of red deer calves and hinds of the Iberian subspecies (Cervus elaphus hispanicus). Milk production was assessed in 14 hinds by weighing calves before and after suckling and by adjusting these values to the Gamma function. Gamma estimates of total milk production up to d 105 were similar to the amounts computed directly from double weighing. Hinds showed two types of lactation curve: 1) the standard mammal lactation curve, with an asymmetrical peak at wk 2 to 4 (Type I) and 2) decreasing curves with no peak (Type II). Although there was great interindividual variability, hinds with Type I curves showed a trend to produce more milk than those with Type II. The type of curve did not seem to affect weight variables of the calf or those of the dam. Calves that gained more weight consumed greater amounts of milk (P<.05). Males were heavier than females at birth (P<.05), but males did not differ from females in their weight at 105 d, milk consumption, and gain. Gender did not affect hind weight, but dams of male calves showed a trend to be lighter (P = .063) at d 105 than dams of female calves. Our results suggest that suckling differences found in other studies between male and female calves may not involve differences in milk production, although other rearing costs seem to affect hind weight losses. They also suggest that the curve type may not depend only, as reported, on the nutrition plane.  相似文献   
15.
Summary When 1, 711 bovine faecal samples from 113 farms in eight dairy areas of Colombia were examined for the presence of helminth eggsFasciola hepatica eggs were found in the faeces from 60% of the farms and samples from animals kept above 2,000 m. Strongyle eggs were found in faeces from 82% of the farms and in 18% of the samples.
Resumen Se examinaron 1.711 muestras fecales provenientes de 113 fincas, localizadas en ocho zonas lecheras de Colombia. Se encontraron huevos deFasciola hapatica, en el 60% de fincas y muestras fecales de animales sobre los 2,000 m.s.n.m. Se encontraron tambien huevos de estrogilideos en heces del 82% de fincas y en el 18% de las muestras examinadas.

Résumé La recherche d’oeufs d’helminthes sur 1711 échantillons de fécès provenant de 113 fermes de 8 districts laitiers de Colombie a permis de noter la présence d’oeufs deFasciola hepatica dans 60 p. 100 des fermes et échantillons originaires d’animaux vivant à plus de 2,000 m. Des oeufs de strongles ont été notés dans les fécès provenant de 82 p. 100 des fermes et 18 p. 100 des échantillons.
  相似文献   
16.
Of 4,144 serum samples collected from cows on 113 farms from eight areas of Colombia 3.3% had positive and 8.8% inconclusive titres to Brucella abortus, 21.7, 6.3, 1.6, 0.6 and 0.7% of cows had positive titres to Leptospira serovars hardjo, pomona, canicola, icterohaemorrhagiae and grippotyphosa respectively. Questionnaires completed on 110 farms revealed that 6, 2.5 and 4.6% of cows had had metritis, aborted or retained their placentas respectively in the previous 12 months. Trichomonas foetus and Campylobacter fetus were isolated from 13.7% and 15% of the bulls sampled on 103 farms. Six and two bulls had inconclusive and positive titres to Brucella abortus. Eight and 23 bulls had positive titres to pomona and hardjo. The results were discussed and remedies for control suggested.  相似文献   
17.
We investigated the therapeutic effects of a progesterone releasing intravaginal device (PRID) on cystic ovarian disease (COD) and reproduction performance of cows. The possible influence of PRID on metabolic and/or health status was also examined. A total of 40 Holstein-Friesian cattle, with ovarian cystic structures, > or =2.5 cm in diameter, persisting for more than 7-14 days, without a corpus luteum (CL) were used for the study. PRID or placebos were inserted into the vagina for 12 days. Five animals lost the intravaginal device before removal and one was culled. Based on plasma progesterone concentration on the day of treatment, 20 (17 PRID and 3 placebos) of the remaining 34 cows had follicular cysts (progesterone < or =1 ng/ml) and 14 (10 PRID and 4 placebos) had luteal cysts (progesterone >1 ng/m l). Fourteen (82%) of the PRID-treated follicular cystic cows responded with formation of a CL within 14 days after treatment, and an overall conception rate of 53.8%. Likewise, 70% of the treated luteal cystic cows responded with CL formation and 71.4% conception rate. No significant differences were observed in hematocrit (Ht), white blood cell count and serum levels of glucose, blood urea nitrogen, aspartate aminotransferase, and alanine aminotransferase, between the day of PRID insertion and removal, in animals with follicular and luteal cysts. PRID treatment resulted in ovulation 2-4 days later and formation of a CL in cows that recovered.  相似文献   
18.
19.
Immunity to Taenia solium infection was investigated using an experimental intramuscular oncosphere infection assay (IMOA) model in pigs. Three naturally infected pigs with cysticercosis were treated with oxfendazole (OFZ), a drug demonstrated to kill cysts in porcine muscle. These animals were then challenged with oncospheres but did not develop any cysts while three uninfected pigs that were similarly challenged, did develop intramuscular cysts. In another study, two groups of three pigs each were immunized with crude T. solium oncosphere and metacestode antigens, respectively, and tested with the IMOA. Immunization with crude oncosphere antigens (OAs) induced 100% protection, while metacestode antigens provided only partial protection. Immunoblots showed that pigs with complete immune protection to oncosphere intramuscular challenge had antibodies to two OAs at 31.3 and 22.5 kDa, respectively. Antibody to these two antigens was absent in pigs immunized with metacestodes or in uninfected control pigs. This study demonstrated the presence of two antigens that are unique to the oncosphere. Although, antibody to these two antigens is consistently present in pigs that are protected from an oncosphere intramuscular challenge their role in preventing infection by T. solium larval cysts is still hypothetical.  相似文献   
20.
Practicing veterinarians in small-animal or mixed-animal practice in New York state were surveyed about their beliefs and practices regarding the age at which dogs and cats should be neutered and their attitudes toward early neutering (at 4 months of age or younger). The majority of veterinarians routinely recommended neutering for all client animals (70.6%) and supported the routine neutering of shelter animals before adoption (90.3%). More veterinarians in this study reported at least one perceived benefit (91.3%) for early neutering than reported at least one perceived risk (84.4%). Veterinarians with experience neutering early were less likely to believe that the procedure was associated with one or more risks.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号