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131.
Bluetongue virus (BTV) is a non-enveloped dsRNA virus that causes a haemorrhagic disease mainly in sheep. It is an economically important Orbivirus of the Reoviridae family. In order to estimate the importance of T cell responses during BTV infection, it is essential to identify the epitopes targeted by the immune system. In the present work, we selected potential T cell epitopes (3 MHC-class II-binding and 8 MHC-class I binding peptides) for the C57BL/6 mouse strain from the BTV-8 non-structural protein NS1, using H2b-binding predictive algorithms. Peptide binding assays confirmed all MHC-class I predicted peptides bound MHC-class I molecules. The immunogenicity of these 11 predicted peptides was then determined using splenocytes from BTV-8-inoculated C57BL/6 mice. Four MHC-class I binding peptides elicited specific IFN-γ production and generated cytotoxic T lymphocytes (CTL) in BTV-8 infected mice. CTL specific for 2 of these peptides were also able to recognise target cells infected with different BTV serotypes. Similarly, using a combination of IFN-γ ELISPOT, intracellular cytokine staining and proliferation assays, two MHC-class II peptides were identified as CD4+ T cell epitopes in BTV-8 infected mice. Importantly, two peptides were also consistently immunogenic in sheep infected with BTV-8 using IFN-γ ELISPOT assays. Both of these peptides stimulated CD4+ T cells that cross-reacted with other BTV serotypes. The characterisation of these T cell epitopes can help develop vaccines protecting against a broad spectrum of BTV serotypes and differentiate infected from vaccinated animals. 相似文献
132.
Sun F Hernández J Ezquerra J Durán E Pineda LF De Miguel C Crisóstomo V Usón J Maynar M 《Journal of the American Animal Hospital Association》2002,38(5):452-457
A case of soft-tissue fibrosarcoma with pulmonary metastases in a dog is reported. Although three attempts of fine-needle aspiration (FNA) biopsy failed to provide definitive tumor diagnosis, results of angiography strongly indicated a soft-tissue sarcoma. Transcatheter arterial embolization (TAE) using particles of gelatin sponge was performed following selective angiography. The mass was decreased in size on reevaluation 2 weeks after embolization. The dog was euthanized on the request of the owners due to overall failing health. Necropsy and pathological study confirmed the diagnosis of soft-tissue fibrosarcoma with pulmonary metastases. In a review of the literature, angiographic findings of soft-tissue sarcoma in the dog of this report were similar to those in human beings, suggesting a potential role for angiography in the differential diagnosis of suspect soft-tissue fibrosarcomas and for guiding FNA or surgical biopsy. Previous reports have also shown therapeutic embolization to be an effective treatment both in experimental animal study and in clinical practice in the human; therefore, TAE could be an effective adjunctive treatment of soft-tissue fibrosarcoma in the dog. 相似文献
133.
Postingel Quirino Patricia da Silva Rodrigues Maira da Silva Cabral Elis Marina de Siqueira-Silva Diógenes Henrique Mori Ricardo Hideo Butzge Arno Juliano Nóbrega Rafael Henrique Ninhaus-Silveira Alexandre Veríssimo-Silveira Rosicleire 《Fish physiology and biochemistry》2021,47(3):747-755
Fish Physiology and Biochemistry - In view of the established climate change scenario and the consequent changes in global temperature, it is essential to study its effects on animal... 相似文献
134.
Lilenbaum W de Souza GN Ristow P Moreira MC Fráguas S Cardoso Vda S Oelemann WM 《Veterinary journal (London, England : 1997)》2007,173(2):408-412
In spite of the large number of goats found in several developing tropical countries, milk production remains unsatisfactory. The occurrence of infectious diseases, such as leptospirosis, brucellosis and caprine arthritis-encephalitis (CAE) may in part be responsible for sub-optimal production. In this study, 1000 serum samples were tested for leptospirosis, 953 for brucellosis and 562 for CAE. All tested flocks presented at least one seroreactive animal for leptospirosis and for CAE. Reactivity to leptospirosis was 11.1%, and serovar hardjo was the most frequently found. Anti-B. abortus agglutinins were found in 0.5% of the samples presented and 14.1% were seroreactive to CAE. Leptospirosis was considered to represent the major infectious problem in the studied goat flocks. The occurrence of infectious diseases in the tested flocks may represent an important factor contributing to the decreased productivity of the animals. These findings may be similar to those observed in other developing countries and require further study to define the relationship between seropositivity and reduced production. 相似文献
135.
Patrícia de Mello Papa Priscilla Nascimento Guasti Bruna De Vita Nathalia Genú Nakazato Leandro Maia Camila de Paula Freitas Dell’Aqua Verônica Flores da Cunha Scheeren Lorenzo Garrido Teixeira Martini Segabinazzi Luiz Roberto Pena de Andrade Junior Luis Fernando Mercês Chaves Silva Endrigo Adonis Braga Araújo Sidnei Nunes Oliveira Frederico Ozanam Papa Fernanda da Cruz Landim-Alvarenga Marco Antonio Alvarenga 《Reproduction in domestic animals》2020,55(4):429-437
Although stem cell therapy is a promising alternative for treatment of degenerative diseases, there are just few reports on the use of stem cells therapy in horse's reproductive system. This study aims to evaluate the effect of intratesticular injection of bone marrow mesenchymal stromal/stem cells (MSCs) in healthy stallions, and its outcome on seminal parameters and fertility. In Experiment 1, 24 stallions were divided into treatment group (TG) and control group (CG). In the TG, an intratesticular application of MSC was performed, and in the CG, only PBS was used. Measurements of testicular volume, surface temperature and Doppler ultrasonography were performed 24 and 48 hr after treatments. Fifteen days after application, the testicles were removed and submitted to histological analysis. In Experiment 2, 3 fertile stallions received similarly treatment with MSCs. Physical examination and sperm analysis were performed weekly during 60 days after treatment, and at the end, semen from one of them was used for artificial inseminations of 6 healthy mares. In Experiment 1, clinical examinations showed no signals of acute inflammation on both groups according to the analysed variables (p > .05). Also, no signal of chronic inflammation was observed on histological evaluation. In Experiment 2, stallions presented no physical alterations or changes in sperm parameters, and a satisfactory fertility rate (83%; 5/6) was observed after AI. The results support the hypothesis that intratesticular application of bone marrow MSCs is a safe procedure, and this could be a promising alternative to treat testicular degenerative conditions. 相似文献
136.
Lustosa-Silva Jéssica Daniele Ferreira-Gomes Regina Lucia Martínez-Castillo Jaime Carvalho Leonardo Castelo Branco de Oliveira Leonardo Furtado Ortiz-García Matilde Margarita Sánchez-Sosa Alba Gabriela da Silva Geice Ribeiro Costa Marcones Ferreira Silva Verônica Brito Lopes Ângela Celis de Almeida 《Genetic Resources and Crop Evolution》2022,69(8):2819-2832
Genetic Resources and Crop Evolution - Lima bean (Phaseolus lunatus L.) is a crop that stands out for its economic and social importance throughout the world, including in Northeast Brazil.... 相似文献
137.
138.
Otaiza-González Santiago N. Mary Verónica S. Arias Silvina L. Bertrand Lidwina Velez Pilar A. Rodriguez María G. Rubinstein Héctor R. Theumer Martín G. 《European journal of plant pathology / European Foundation for Plant Pathology》2022,162(1):203-219
European Journal of Plant Pathology - Fusarium graminearum and Zymoseptoria tritici cause economically important diseases of wheat. F. graminearum is one of the primary causal agents of Fusarium... 相似文献
139.
Almazán-Sánchez Perla Tatiana Marin-Noriega Paul William González-Mora Eduardo Linares-Hernández Ivonne Solache-Ríos Marcos J. Martínez-Cienfuegos Iván G. Martínez-Miranda Verónica 《Water, air, and soil pollution》2017,228(8):1-26
Water, Air, & Soil Pollution - Biochar presents great promise as a technology that makes a substantial contribution in various fields of environmental research. However, existing knowledge is... 相似文献