Anti-viral activity of methyl 1-chloro-7-methyl-2-propyl-1h-benzo[d] imidazole-5-carboxylate against white spot syndrome virus in freshwater crab (Paratelphusa hydrodromous)

Shrimp farming industries are subjected to severe economic loss due to a disease called white spot syndrome, a viral disease caused by white spot syndrome virus (WSSV) in penaeid shrimp. Numerous active compounds in the market possess anti-viral activity against the white spot syndrome virus, yet the issue remains unsolved. The present study was carried out to determine the anti-viral activity of methyl 1-chloro-7-methyl-2-propyl-1h-benzo[d] imidazole-5-carboxylate (C₁₃H₁₅N₂O₂Cl) against WSSV. The anti-viral activity of the synthetic compound was determined in freshwater crabs. Crabs were divided into three different experimental groups: healthy control groups (N.C.) received NTE buffer, positive control group (P.C.) crabs received WSSV, and treatment group crabs received WSSV along with synthetic weight compound. Experimental groups were observed for 30 days post-infection. Three different organs (gills, muscles, and head soft tissue (HST)) were dissected from all three groups and analyzed using molecular-based techniques, including polymerase chain reaction (PCR), Western blot, and histopathology. Clinical signs of WSSV were observed in the positive and N.C. groups; however, the treatment group showed a 100% survival rate. Confirmation was done using PCR, Western blot, and histopathology. These results demonstrated that the given synthetic compound has significant anti-viral activity against WSSV.

     

Genetic transformation of pigeonpea with rice chitinase gene

With a long‐term plan to develop transgenic pigeonpea with resistance to fungal disease, the transfer of a rice chitinase gene to pigeonpea [Cajanus cajan (L.) Millsp.] is reported here. The rice chitinase gene harboured in the plasmid pCAMBIA 1302:RChit was delivered via the Agrobacterium‐mediated method to the cotyledonary node explants followed by subsequent regeneration of complete plants on selection media containing hygromycin. Putative transformed pigeonpea plants were recovered with stringent selection pressure and confirmed using molecular techniques. Stable integration and expression of the chitinase gene has been confirmed in the T0 and T1 transgenics through molecular analysis.     

Rice bran lysolecithin as a source of energy in broiler chicken diet

1. Rice bran lysolecithin (RBL) was evaluated in broiler chicken diets. In the first experiment, RBL was included in diet at 0, 0·5, 2, 8 and 32?g/kg and fed to 250 broiler chickens from 0 to 42?d of age. In the second experiment, RBL was fed at 0, 25 and 50?g/kg diet to 405?day-old broiler chickens until 21?d of age, while during the finisher phase (22–35?d of age) chickens receiving each concentration of RBL were given all three concentrations of RBL in a 3?×?3 factorial manner. The diets were isocaloric.

2. Body weight, food consumption and food conversion efficiency were unaffected by feeding RBL, while the weight of pancreas increased at ≥2?g/kg of RBL in diet (experiment 1). In experiment 2, body weight was greater in the chickens receiving RBL at either 25 or 50?g/kg (21?d) and 50?g/kg (35?d of age). At 21?d of age, food consumption was greater at 25 or 50?g RBL/kg diet, while food conversion efficiency improved with 50?g RBL/kg diet.

3. Fat digestibility increased with RBL at 32?g/kg (experiment 1) and ≤25?g/kg (experiment 2). Rice bran lysolecithin increased ready to cook weight at 50?g/kg during starter phase and decreased abdominal fat at 25 and 50?g/kg during finisher phase (experiment 2). Liver and meat fat content were not affected.

4. It is concluded that lysolecithin from rice bran oil could be used as energy supplement in broiler chicken diet.     

Arsenic-prone rice cultivars: a study in endemic region

Rice is prone to arsenic accumulation compared to other cereals as typically grown up under waterlogged situation favoring arsenic mobility. Arsenic in rice depends on arsenic availability to plants from irrigation water, even differs among cultivars and their plant parts. Present study was concentrated on arsenic accumulation in various plant parts of five common rice cultivars grown using irrigation water from different water sources in various fields in arsenic-endemic region. Additionally, dose response experiment under laboratory net house was conducted on the same cultivars excluding open environmental factors. The common cultivars were categorized according to high to low arsenic accumulator in rice grain, straw and root parts. The cultivar Shatabdi has shown highest arsenic accumulation in rice grain compared to other rice cultivars in fields and when grown at various soil arsenic doses. In field samples, a highest grain arsenic concentration ranged between 0.69 ± 0.04 and 0.78 ± 0.12 mg kg^?1 for Shatabdi, whereas lowest grain arsenic concentration ranged between 0.37 ± 0.07 and 0.41 ± 0.07 mg kg^?1 for the cultivars GB3 and Lalat. Speciation study detected more inorganic arsenic than organoarsenicals with a trend of arsenite > arsenate > DMA > MMA, which would be problem for consumers. The concluding remark is the characterization of common rice cultivars according to arsenic concentration to highlight an important remediation strand by changing to low arsenic cultivar.     

Hypocholesterolemic effect of amaranth seeds (Amaranthus esculantus)

Food lectins are known to affect the absorption of nutrients in experimental animals and altered morphology of the intestinal mucosa due to prolonged feeding of lectins is suggested to be the cause. However, toxic symptoms appear in humans immediately after consumption of lectin rich foods and the mechanism of this immediate action is not known. This study reports the immediate effect of Concanavalin A (Con A) and Phaseolus vulgaris lectin (PHA-P) on the absorption of Ca²⁺ and sucrose in rat intestine and the possible role of lectin-membrane interactions in the process. Calcium absorption was lowered by Con A, and this was observed to be due to Con A interacting with the intestinal mucosal membrane. Similarly, sucrose absorption was also altered, probably due to Con A binding the intestinal mucosal membrane. On the other hand PHA-P did not have any effect on either sucrose or calcium absorption. Results indicate that the effect on intestinal nutrient absorption depends on the type of lectin used (and its concentration) as well as the type of nutrient, besides the duration of exposure of intestinal mucosa to the lectin.     

Sterol Ring System Oxidation Pattern in Marine Sponges

The marine sponges (Porifera) are a unique group of sedentary organisms from which several novel natural products are reported, many of which have useful biological activities. In producing unusual sterols, they occupy a preeminent position among the various groups of organisms. The polar sterols of sponges reported as at the end of the year 2002 number about 250; their ring structure changing a hundred times. The oxidation pattern in the sterol ring system, from the point of view of biogenesis seems to be mainly of four types. Each sponge species is able to produce sterols fitting into one of the four main biogenetic pathways viz., (i) 3β-hydroxy-Δ⁵-sterol pathway, (ii) 3β-hydroxy-Δ⁷-sterol pathway, (iii) 3β-hydroxy-Δ^5,7-sterol pathway, and (iv) 3α-hydroxy sterol pathway.     

Trends in Information Needs and Communication Channel Use Among Rural Women in Africa,Asia, and Latin America, 2000–2012

A trend analysis of information needs and communication channel use of rural women in Africa, Asia, and Latin America was conducted by examining empirical works published in reports, scholarly publications, and the popular press from 2000 to 2012. Results show that information about farming practices, health, education, gender and general family well-being issues were the most sought across the three continents. Demand for 14 information categories surged in volume and scope after 2009. Interpersonal communication sources trumped the mediated ones as the channels of choice; extension agents were the most preferred source. Radio was the most frequently mentioned preferred medium.     

Effect of feeding of calcium hydroxide-treated or vitamin E-supplemented cottonseed meal on plasma gossypol levels, blood parameters, and performance of Bikaneri lambs

To study the effect of feeding calcium hydroxide-treated or vitamin E-supplemented cottonseed meal (CSM) incorporated diets on plasma gossypol, blood parameters and animal performance, 24 male Bikaneri lambs of 6–7 months of age and of uniform body weight were divided into four groups of six animals each in a completely randomized design and respectively fed isonitrogenous and isocaloric concentrate mixtures containing 20 % soybean meal (CON) or 40 % raw CSM (RCSM), 40 % raw CSM supplemented with 500 IU of vitamin E per head per day (ERCSM), and 40 %, 1.5 % calcium hydroxide-treated CSM (CaCSM) along with ad libitum wheat straw throughout 510 days of experimental feeding. The lambs on all the diets grew linearly throughout the experimental period. The total weight gain, in turn the average daily gain (ADG), was not affected by dietary variations. The daily intake of dry matter, crude protein (CP), digestible crude protein (DCP) and total digestible nutrients (TDN) were found comparable among lambs of all the groups. Though total gossypol intake was similar in RCSM, ECSM and CaCSM groups, however, free gossypol intake was significantly higher (P?<?0.01) in RCSM, ECSM groups as compared to CaCSM group. Serum iron and blood hemoglobin levels were significantly (P?<?0.05) lower in RCSM group as compared to CaCSM and CON groups, and ALT activity was significantly (P?<?0.05) higher on RCSM group as compared to other groups. Plasma gossypol and osmotic fragility of erythrocytes were significantly (P?<?0.05) increased in RCSM group as compared to CaCSM and ERCSM groups. However, there was no significant difference in the concentration of other blood/serum biochemical constituents among the lambs on different groups. Based on the results, it may be concluded that feeding of 40 % CSM in the concentrate mixture of the diet in Bikaneri lambs did not have any major adverse effect in blood parameters and animal performance. Either calcium hydroxide treatment or vitamin E supplementation did not produce any major additional benefits.     

Effect of supplementing different concentrations of organic trace minerals on performance, antioxidant activity, and bone mineralization in Vanaraja chickens developed for free range farming

An experiment was conducted to determine the performance, antioxidation activity, and bone mineral content in Vanaraja chickens fed diet supplemented with organic trace minerals (oTM) at reduced levels. A total of 360 day-old chicks were selected and distributed randomly into 60 battery brooder pens. A maize–soybean meal-based control diet was supplemented with inorganic trace minerals (iTM), i.e., Mn, Zn, Fe, and Cu at 50, 45, 40, and 7.5 mg/kg, respectively, and varying concentration of oTM, i.e., Zn, Mn, Cu, Fe, I, Se, and Cr at 45, 50, 7.5, 40, 2, 0.30, and 0.25 mg/kg (diet II); 33.75, 37.50, 5.63, 30.0, 1.50, 0.23, and 0.19 mg/kg (diet III); 22.5, 25.0, 3.75, 20.0, 1.0, 0.15, and 0.13 mg/kg (diet IV); 18.0, 20.0, 3.0, 16.0, 0.80, 0.12, and 0.10 mg/kg (diet V); and 13.5, 15.0, 2.25, 12.0, 0.60, 0.09, and 0.08 mg/kg (diet VI), respectively. Each diet was allotted randomly to ten replicates and fed ad libitum from 1 to 42 days of age. The body weight at 14, 28, and 42 days was not affected by reducing the supplementation of oTM concentration in the diets. Similarly, feed intake at 14 days of age was not affected but reduced significantly (P?<?0.05) in the group fed diet IV (50 % oTM) compared to that in the other groups. The higher feed conversion ratio and increased concentration of Ca, P, and trace minerals in tibia were evident in the group fed oTM-supplemented diets compared to the diet containing iTM. Activities of glutathione peroxidase and ferric reducing ability in plasma did not differ in the groups fed on lower concentration of oTM compared to those fed on diet I (control diet). Therefore, it is concluded that the dietary supplementation of trace minerals can be reduced greatly when supplemented as organic form without affecting growth and antioxidant status in Vanaraja chickens.