The genetic relationship between popping expansion volume and two yield components in popcorn using unconditional and conditional QTL analysis

Popping expansion volume (PEV) is the most important quality trait in popcorn, while its germplasm is inferior to normal dent/flint corn in yield. In this study, 259 F_2:3 families, developed from the cross between a dent corn inbred Dan232 and a popcorn inbred N04, were evaluated for their PEV, grain weight per plant (GWP) and 100-grain weight (100 GW) in two environments. The genetic relationship between PEV and GWP, and 100 GW on individual gene loci were evaluated using unconditional and conditional QTL mapping methods. In total, five, one and three unconditional QTL were identified for PEV, GWP, and 100 GW, respectively. The positive alleles of all QTL for PEV were from N04, while positive alleles of all QTL for GWP and 100 GW were from Dan232. In conditional mapping, one and two QTL failed to be detected, and all four additional QTL were detected. Nevertheless, three QTL were identified, which controlled PEV independently from GWP/100 GW. They seemed to be potential candidates in popcorn breeding to increase PEV without decreasing GWP/100 GW. The results suggested that for significantly correlated traits, the conditional QTL mapping method could be used to dissect the genetic interrelationship between traits at the level of individual QTL, as well as reveal additional QTL that were undetectable by unconditional mapping.     

Effects of plastic film mulch and tillage on maize productivity and soil parameters

This paper determined the effects of mulching time for double furrows and ridges using plastic film on soil water status, grain yield of maize, soil quality, and economic benefits. The study was conducted in a typical semiarid area during two growing seasons of 2006–2007 with the following three treatments: (i) plastic film mulching at maize sowing with conventional tillage, and the film was removed at harvest (CK); (ii) mulching applied 30 d before sowing with conventional tillage, and the film was removed at harvest (T1); and (iii) mulching at sowing with no-tillage, and the film left on the field after harvest in the first season and used for mulching in the second season (T2). The T1 in both years and T2 in the second year (2007) improved soil water content (in the 0–60 cm layer) and temperature (10 cm) at sowing compared with CK. After the two seasons, the soil water content was significantly higher in the 0–80 cm soil layer in CK and T1, and in the 0–120 cm soil layer in T2; however, it decreased significantly in 140–200 cm soil layer in CK and T1, compared to their initial values at sowing in April 2006, and there was no significant change in T2. The rainfall storage in the 0–200 cm soil layer during the non-growing season (late September 2006 to late April 2007) was 18.2 mm in CK, 34.0 mm in T1, and 59.7 mm in T2, and the rainfall storage in 100–200 cm soil layer was 16.5 and 18.6 mm higher in T2 than in CK and T1, respectively. In 2006, there were no significant differences in yield and water use efficiency (WUE) in all treatments. In 2007, the yield in T1 was significantly higher than in T2, but yields in T2 and CK were not significantly different, and there was no significant difference in WUE among treatments. Soil organic carbon (SOC) (0–20 cm) decreased in CK and T1, but increased (by 2.7%) in T2 at harvesting in September 2007 from the initial value of sowing in April 2006. The ratio of output to input was 1.32:1 for CK, 1.40:1 for T1, and 1.67:1 for T2 averaged across the two seasons. Therefore, T2 was a more sustainable model for increasing water storage, producing greater economic benefit and maintaining SOC balance for maize production in semiarid area.     

考虑气候分区的甘肃省干旱时空分布特征分析

甘肃省地处生态脆弱区,气候条件复杂,干旱发生概率高、范围广。为了更好地研究甘肃省干旱时空变化特征,综合考虑甘肃省气候类型和地理特征将其划分为4个气候分区（Ⅰ区,河西大陆性气候区;Ⅱ区,陇中北部季风气候区;Ⅲ区,陇南-陇中南部季风气候区;Ⅳ区,甘南高寒气候区）,并采用甘肃省26个国家气象站点的气象资料,计算其近60年（1960—2019年）的月、季和年尺度的标准化降水蒸散指数（SPEI-1、SPEI-3、SPEI-12）,结合气候倾向率、Mann-Kendall突变检验、空间插值等方法探讨甘肃省近60年的干旱时空演变特征。结果表明：从时间变化角度看,不同时间尺度的SPEI均呈减小变化趋势,且随时间尺度的增大,SPEI波动幅度越小;在四季变化上,春、夏、秋季SPEI在甘肃省各气候分区都呈现在波动中下降的趋势,且下降趋势明显,表明干旱趋势显著,冬季SPEI在各气候分区呈现在波动中上升的趋势,表明有湿润化的趋势。从空间变化角度看,甘肃省Ⅰ区呈干旱减缓趋势,Ⅱ区、Ⅲ区和Ⅳ区呈干旱加剧趋势,且春季各气候分区干旱加剧趋势明显,夏、秋季次之,而冬季基本上都呈现干旱减缓趋势;甘肃省不同气候分区不同等级干旱发生频率分布差异大且不均衡,干旱频率由小到大依次为：特旱、中旱、重旱、轻旱。     

A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.)

The root-knot nematodes (Meloidogyne spp.) are important nematode pests and cause serious diseases in pepper in the world. No molecular markers linked to the nematodes resistance N gene have been reported. In this paper, ‘Carolina Wonder’ (Capsicum annuum L.), a sweet pepper line resistant to root-knot nematode with N gene, ‘20080-5-29’ (C. annuum L.), an inbred line susceptible to root-knot nematode with good horticultural characteristics, and their F₂ progeny with 320 individuals were used as materials. Evaluation of resistance and susceptibility of parental lines, F₁ and F₂ progeny inoculated with root-knot nematodes (Meloidogyne incognita) were carried out. ‘Bulked segregant analysis’ method was used to search for polymorphic markers from 512 pairs of AFLP primers. Based on the assessment of resistance and susceptibility and polymorphism of the AFLP marker in F₂ population, the genetic linkage distance between the AFLP marker and the N gene was estimated. One AFLP marker E39/M41-339 was obtained and transferred to a SCAR marker amplifying a 315 bp DNA fragment linked to the N resistant allele and a 331 bp fragment linked to the N⁺ susceptible allele. The distance between the molecular marker and the nematodes resistance N gene is 6.3 cM. This research delivered a valuable tool for the marker assisted selection of nematodes resistance in pepper.     

Modeling the water budget in a deep caldera lake and its hydrologic assessment: Lake Ikeda, Japan

The hydrologic assessment of a lake water budget can be helpful in achieving proper water management and sustainable water use. A model to analyze a lake water budget was developed and verified for Lake Ikeda, Japan. Lake evaporation was estimated by numerical analyses of lake water temperature and the lake energy budget. Inflow from the lake catchment area and leakage from the lake bottom were estimated based on the tank model and Darcy's law, and the model parameters were optimized by the shuffled complex evolution method. The estimated monthly lake evaporation rate is consistent with the evaporation rate estimated by the energy budget Bowen ratio method based on in situ data from 2004 to 2005. Moreover, the calculated time series of daily lake levels agrees well with those of measured lake levels during 1983 to 1999. Thus, the model is useful for evaluating the lake water budget. Numerical analysis reveals seasonal and annual variation characteristics in the water budget components. Precipitation, inflow from the catchment area, and river water supply are generally high during the rainy season from June to July with substantial annual variation. Lake evaporation is greatest in October and least in April, but the annual variation is relatively small. Agricultural water use is relatively high from April to September. There are no marked seasonal changes in leakage and drinking water use. The lake level is generally highest in September and lowest in March, which is characterized by seasonal changes in water budget components. The model was also applied to 17-year simulations under hypothetical hydrologic conditions to examine the effect of water use and agricultural water management on the lake level. Results indicate that river water supply, provided under the agricultural water management system, effectively compensates for the decrease in lake water resulting from agricultural water use.     

Mineral content of three olive cultivars irrigated with treated industrial wastewater

The reuse of saline treated industrial wastewater generated by textile firms mixed with municipal domestic effluent for irrigation was used to asses its effect on the mineral content of three olive (Olea europaea L.) cultivars under greenhouse and field conditions during two complete vegetative cycles. Chemical analysis of the treated wastewater indicated that the element concentrations fall within the permissible range of irrigation water used for plants. However, little impermissible accumulation of Na and Mg higher than the recommended maximum concentration was observed. Irrigation water with six electrical conductivities (EC = 0.78, 1.0, 2.0, 3.0, 4.0 and 5.0 dS m⁻¹ in treatments T₀, T₁, T₂, T₃, T₄, T₅, respectively) were compared in the greenhouse experiment. The olive trees in the field experiment were trickle irrigated with potable water and treated wastewater (average EC = 4.2 dS m⁻¹). The results of the greenhouse experiment showed that leaf N, Cu, Mn, Fe, Pb, and Na contents increased with increasing salinity of the treated wastewater. This increase was accompanied with a decrease in K and Mg contents. Leaf Ca and Cl concentrations were not considerably affected. Ion analysis in roots indicated that the contents of P, Na, Cl, Mn, and Pb increased while K decreased as treated wastewater salinity increased. Consequently, in most cases T₄ and T₅ gave a highly significant increase or decrease in accumulation of the previously mentioned minerals. A considerable variation in the studied cultivars was noticed. ‘Nabali’ was considered the most tolerant cultivar for the high salinity levels of the treated wastewater; its transporting selectivity of Na from root to leaf was higher and more Na was retained in the roots. Tissue analysis of leaves indicated that the element concentrations were within the adequate levels except those of Fe in ‘Nabali’ and ‘Manzanillo’, Na in ‘Improved Nabali’ and Cu in ‘Nabali’ and ‘Manzanillo’. In view of these findings, the negligible accumulation of minerals in leaves and roots indicated that this kind of textile effluent can be used as a valid alternative for irrigation of olive orchards with continuous monitoring of mineral levels.     

FSWM: A hybrid fuzzy-stochastic water-management model for agricultural sustainability under uncertainty

A hybrid fuzzy-stochastic water-management (FSWM) model is developed for agricultural sustainability under uncertainty, based on advancement of a multistage fuzzy-stochastic quadratic programming (MFSQP) approach. In MFSQP, uncertainties presented in terms of fuzziness and randomness can be incorporated within a multilayer scenario tree, such that revised decisions are permitted in each time period based on the realized values of the uncertain events. Moreover, fuzzy quadratic terms are used in the objective function to minimize the variation of satisfaction degrees among the constraints; it allows an increased flexibility in controlling the system risk in the optimization process. Results of the case study indicate that useful solutions for the planning of agricultural water management have been obtained. In the FSWM model, a number of policies for agricultural water supply are conducted. The results obtained can help decision makers to identify desired water-allocation schemes for agricultural sustainability under uncertainty, particularly when limited water resources are available for multiple competing users.     

Evaluation of surface water drainage systems for cropping in the Central Highlands of Ethiopia

In Ethiopia vertisols cover about 10% of the total land area and is the fourth most important soil used for crop production, accounting for nearly 23% of the total arable land used for crop production. More than half of the vertisols are found in the Central Highlands of Ethiopia, with an altitude of more than 1500 m above mean sea level. The unique physical and chemical properties of these soils and the high rainfall during the main cropping season create severe surface waterlogging problems which hinder crop production activities. Severe surface waterlogging affects the growth of plants by impeding nutrient uptake and creating oxygen deficiency around the root zone. To address this crop production problem, three surface water drainage methods, namely broad bed and furrow (BBF), ditch, and flat (traditional) methods were evaluated using the water balance of the plant root zone and wheat as a test crop. The experiment was conducted at the Ginchi Research Station in the central highlands of Ethiopia over two consecutive seasons (2000 and 2001). The results showed that both the BBF and the ditch drainage methods gave about 33% and 22% more grain yield than the flat treatment, respectively. However, there were no significant differences between BBF and ditch for both grain and biomass yield during both experimental seasons. During both seasons the total water balance (ΔW_r) at the root zone especially, in the months of June, July and August on all the treatments was higher than the crop water requirement (ETc) and showed no significant difference between the treatments. Thus, the results of this study indicated that the soil water in the root zone was not significantly altered by surface drainage systems and therefore implies the need of further improvement of the different surface drainage methods regarding improving the waterlogging condition and hence the productivity of the vertisols in the Central Highlands of Ethiopia.     

Growth and body composition of juvenile white shrimp,Litopenaeus vannamei,fed different ratios of dietary protein to energy

A 10‐week feeding experiment was conducted to evaluate the effect of different protein to energy ratios on growth and body composition of juvenile Litopenaeus vannamei (initial average weight of 0.09 ± 0.002 g, mean ± SE). Twelve practical test diets were formulated to contain four protein levels (300, 340, 380 and 420 g kg^?1) and three lipid levels (50, 75 and 100 g kg^?1). Each diet was randomly fed to triplicate groups of 30 shrimps per tank (260 L). The water temperature was 28.5 ± 2 °C and the salinity was 28 ± 1 g L^?1 during the experimental period. The results showed that the growth was significantly (P < 0.05) affected by dietary treatments. Shrimps fed the diets containing 300 g kg^?1 protein showed the poorest growth. However, shrimp fed the 75 g kg^?1 lipid diets had only slightly higher growth than that fed 50 g kg^?1 lipid diets at the same dietary protein level, and even a little decline in growth with the further increase of dietary lipid to 100 g kg^?1. Shrimp fed the diet with 420 g kg^?1protein and 75 g kg^?1 lipid had the highest specific growth rate. However, shrimp fed the diet with 340 g kg^?1 protein and 75 g kg^?1 lipid showed comparable growth, and had the highest protein efficiency ratio, energy retention and feed efficiency ratio among dietary treatments. Triglycerides and total cholesterol in the serum of shrimp increased with increasing dietary lipid level at the same dietary protein level. Body lipid and energy increased with increasing dietary lipid level irrespective of dietary protein. Results of the present study showed that the diet containing 340 g kg^?1 protein and 75 g kg^?1 lipid with digestible protein/digestible energy of 21.1 mg kJ^?1 is optimum for L. vannamei, and the increase of dietary lipid level has not efficient protein‐sparing effect.     

Acetyl- and butyrylcholinesterase inhibitory activities of sterols and phlorotannins from Ecklonia stolonifera

ABSTRACT:   As part of this study on the isolation of cholinesterase inhibitors from natural marine products, the bioactivity of the ethanolic extracts from 27 Korean seaweeds were screened using acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory assays. Ecklonia stolonifera exhibited promising inhibitory properties against both AChE and BChE. Bioassay-guided fractionation of the active n -hexane and ethyl acetate (EtOAc) soluble fractions, obtained from the ethanolic extract of E. stolonifera , resulted in the isolation of the sterols; fucosterol ( 1 ) and 24-hydroperoxy 24-vinylcholesterol ( 2 ), from the n -hexane fraction and the phlorotannins; phloroglucinol ( 3 ), eckstolonol ( 4 ), eckol ( 5 ), phlorofucofuroeckol-A ( 6 ), dieckol ( 7 ), triphlorethol-A ( 8 ), 2-phloroeckol ( 9 ) and 7-phloroeckol ( 10 ), from the EtOAc fraction. Of these, compounds 2 , 9 and 10 were isolated from E. stolonifera for the first time. Compounds 4 – 7 , 9 and 10 exhibited inhibitory potential against AChE, with 50% inhibition concentration (IC₅₀) values of 42.66 ± 8.48, 20.56 ± 5.61, 4.89 ± 2.28, 17.11 ± 3.24, 38.13 ± 4.95 and 21.11 ± 4.16 μM, respectively; whereas, compounds 1 , 2 , 4 and 6 were found to be active against BChE, with IC₅₀ values of 421.72 ± 1.43, 176.46 ± 2.51, 230.27 ± 3.52 and 136.71 ± 3.33 μM, respectively. It has been suggested that the inhibition of these enzymes by the sterols and phlorotannins derived from marine brown algae could be a useful approach for the treatment of Alzheimer's disease.