全基因组SNP分型技术在畜禽遗传育种研究中的应用

随着畜禽资源分子鉴定、物种进化、全基因组育种等热点领域的逐渐兴起,准确的全基因组SNP分型成为了畜禽基因组研究的关键。基因芯片、重测序、简化基因组测序及靶向捕获测序等全基因组SNP分型技术已广泛应用于畜禽基因组研究中。本文概述了全基因组SNP分型技术的原理及其在全基因组关联分析、选择信号分析和畜禽遗传资源背景分析等方面的应用,以期为畜禽基因组研究和育种应用提供借鉴和参考。     

萃取大红袍花椒挥发油的研究

利用超临界CO2萃取装置，采用动态萃取的方法，研究了压力、温度、萃取时间等因子对花椒挥发油萃取的影响，为规模化生产及其相关研究提供了科学依据。研究表明，样品200g粒度40目，最佳的萃取条件为：压力33MPa，温度55℃，时间1．0h，花椒挥发油萃取率为10．2％。气-质联用分析花椒挥发油结果表明，挥发油为3-甲基-2氮杂芴、γ-萜品烯、3-蒈烯等31种化舍物组成。其中3-甲基-2-氮杂芴含量最高，达到18．63％。     

赤峰市木本植物资源的研究

赤峰市共有木本植物278种,隶属于44科,92属.其中裸子植物3科,7属,17种;被子植物41科,85属,261种;野生植物种222种,常见栽培种56种.     

Red deer (Cervus elaphus hispanicus) management in the dehesa system in Central Extremadura,Spain

In this paper we report the results of a study on the suitability of the dehesa (Mediterranean woodlands) as habitat of red deer (Cervus elaphus hispanicus) in a closed country estate of about 4300 ha total surface situated in the San Pedro Mountain, in Central Extremadura, Spain. We have described the deer management with respect to habitat, exploitation mode, deer feeding, population control and production of goods and services. It is concluded that the dehesa system is a rich natural resource that preserves and improves the degraded environment.

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Resumen En el presente trabajo se estudia la dehesa como hábitat del ciervo (Cervus elaphus hispanicus). Se ha tomado como referencia una finca cerrada de 4300 ha de ST situada en la Sierra de San Pedro, en Extremadura, España. Se describen los sistemas de gestión actuales del ciervo en cuanto a hábitat, modo de explotación, alimentación, control de población y producción de bienes y servicios. Concluyendo con la necesidad de fomentar este tipo de explotaciones cinegéticas de dehesa que ayudan a conservar y potenciar el medio ambiente en zonas despobladas y deprimidas

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Abbreviations CS collected surface - DMFCU deedmixed feed cattle units - Fi final inventory - FS fertilized surface - Ii Initial inventory - K Potassium - N Nitrogen - NBF number of breeding females - NBFIi number of breeding females of initial inventory - NBFR number of breeding females released - NBM number of breeding males - NBMIi number of breeding males of initial inventory - NBMR number of breeding males released - NCCR number of captive calves released - NCFi number of calves of final inventory - NCR number of calves released - NFR number of female renovation - NYMR number of young males released - NYFR number of young females released - P phosphorus - PP permanent Pastures - pts pesetas - QS Quercus stalks - RDED resources directly eaten by deer - SPOM selected hunted down poor quality males - SS sowed surface - TN total needs of deer - TNR total number released - UAS useable agrarian surface - WB wooded brush - WQS wooded Quercus surface - WS wooded surface     

内蒙古典型草原不同放牧强度下羊草光合生理生态特性分析

通过对内蒙古典型草原不同放牧强度下羊草光合日动态、叶绿素荧光参数以及光合生理生态指标的测定,分析探讨了放牧对羊草光合及生理生态特性的影响。结果表明:羊草净光合速率、蒸腾速率、气孔导度随放牧强度的增大而增大,T2放牧强度下羊草光合日动态为双峰曲线,出现"午休现象",非气孔因素是主要原因;叶绿素荧光参数Fv/Fm三种处理间差异不显著,ΦPSII、qP、ETR三种参数随放牧强度的增大而增大,T2放牧强度下的值显著大于T0和T1;T1放牧强度下羊草相对叶绿素含量显著小于T0和T2,叶干物质含量则相反,比叶面积和干物质含量二者呈负相关关系;羊草叶片数、叶片长度随放牧强度的增大而下降。由此表明,适度放牧有助于羊草光合作用,叶片形态出现缩小特征,以适应放牧干扰。     

Neotropical Bats from Costa Rica harbour Diverse Coronaviruses

Bats are hosts of diverse coronaviruses (CoVs) known to potentially cross the host–species barrier. For analysing coronavirus diversity in a bat species‐rich country, a total of 421 anal swabs/faecal samples from Costa Rican bats were screened for CoV RNA‐dependent RNA polymerase (RdRp) gene sequences by a pancoronavirus PCR. Six families, 24 genera and 41 species of bats were analysed. The detection rate for CoV was 1%. Individuals (n = 4) from four different species of frugivorous (Artibeus jamaicensis, Carollia perspicillata and Carollia castanea) and nectivorous (Glossophaga soricina) bats were positive for coronavirus‐derived nucleic acids. Analysis of 440 nt. RdRp sequences allocated all Costa Rican bat CoVs to the α‐CoV group. Several CoVs sequences clustered near previously described CoVs from the same species of bat, but were phylogenetically distant from the human CoV sequences identified to date, suggesting no recent spillover events. The Glossophaga soricina CoV sequence is sufficiently dissimilar (26% homology to the closest known bat CoVs) to represent a unique coronavirus not clustering near other CoVs found in the same bat species so far, implying an even higher CoV diversity than previously suspected.     

基于改进YOLO v7的生猪群体体温热红外自动检测方法

针对当前生猪规模化养殖过程中基于热红外技术的生猪体温测量效率低的问题,提出了一种基于改进YOLO v7的生猪群体体温检测方法。改进YOLO v7算法在Head层引入VoV-GSCSP结构,降低网络结构复杂度;使用内容感知特征重组(Content-aware reassembly of features,CARAFE)替换模型原始上采样算子,提高特征图放大后的品质,强化生猪头部区域有效特征;引入感受野增强模块（Receptive field enhancement module,RFE）,增强特征金字塔对生猪头部特征的提取能力。本文改进YOLO v7算法对于生猪头部的检测精确率为87.9%,召回率为92.5%,平均精度均值（Mean average precision,mAP）为94.7%。与原始YOLO v7相比,精确率提高3.6个百分点,召回率提高7.0个百分点,mAP提高3.6个百分点。该方法首先自动检测生猪头部区域,再利用头部最大温度与耳根温度的高相关性,最终自动获取生猪体温。温度提取平均绝对误差仅为0.16℃,检测速度为222f/s,实现了生猪群体体温的实时精准检测。综合上述试验结果表明,该方法能够自动定位生猪群体的头部区域,满足生猪群体体温测定的高效和高精度要求,为群养生猪体温自动检测提供了有效的技术支撑。     

秸秆覆盖条件下滨海盐渍土水盐分布及蒸发特征

为了揭示秸秆覆盖滨海盐渍土水盐调控机理,通过室内模拟实验研究了不同秸秆覆盖量(0、0.3、0.6、0.9和1.2 kg/m²分别由CK、秸秆A、秸秆B、秸秆C和秸秆D表示)对土壤水盐运移和蒸发强度的影响。结果表明,秸秆覆盖可有效提高表层及土壤剖面含水率,且增墒效果随秸秆覆盖量的增加而增加:试验期间秸秆A、秸秆B、秸秆C和秸秆D剖面平均含水率比CK依次高了41.2%、52.3%、65.7%和58.5%;秸秆覆盖可抑制表层土壤盐分积聚并有效调控土壤剖面盐分分布,秸秆覆盖量越大表层积盐量越低,土壤剖面盐分分布越趋于均衡:试验结束时,CK、秸秆A、秸秆B、秸秆C和秸秆D表层0~2 cm土壤电导率比3~5 cm电导率分别高了246.3%、242.8%、138.4%、40.5%和47.6%;土壤蒸发强度和累积蒸发量随着秸秆覆盖量的增加而降低:试验期间CK、秸秆A、秸秆B、秸秆C和秸秆D处理平均蒸发强度依次为1.79×10^-3、1.64×10^-3、0.93×10^-3、1.35×10^-3和0.76×10^-3mm/min,累积蒸发量分别为17.79、20.30、14.20、14.57和10.27 mm,且蒸发初期秸秆覆盖对蒸发强度和累积蒸发量的抑制作用更明显。     

PRRSV山西株ORF5和Nsp2基因序列分析

采用RT-PCR方法对2009—2011年山西省分离的5株猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus,PRRSV）的ORF5和Nsp2（2503～3269nt）基因进行克隆和测序,并对其基因序列和推导的氨基酸序列与国内外毒株进行了同源性分析。序列分析结果显示,5株分离株Nsp2基因与国内分离的PRRSV变异株（JXA1、HuN、HUN4、HUB1）的序列同源性最高,为96．8％～98．2％,且缺失位置一致,均存在2个位点30个氨基酸缺失;ORF5基因大小为603bp,编码200个氨基酸,第13、151位均为具有强毒特性的精氨酸（R）,137位为丝氨酸（S）,表明这5株均为野毒株,与国内分离的PRRSV变异株（JXA1、HuN、HUN4、HUB1）毒株的序列同源性最高,为96．5％～98．0％。结果表明,山西省内目前流行的PRRSV为Nsp2缺失30个氨基酸的变异毒株。     

便携式B超在母猪早期妊娠诊断中的应用研究简

The pregnancy rates at 20 and 50 days were tested by sampling 91 hybridization sows more than 16 days using the intuitive, fast, accurate and non-invasive portable veterinary B ultrasound instrument in the pig breeding farm had 300 sows. The results showed that the nonpregnant diagnostic accuracy rate was 100% at 18 to 20 days, and the pregnant diagnostic accuracy rate was 100% at 40 to 50 days. According the diagnostic results, the pregnancy, disease, estrus, nonpregnancy and non-estrused sows were took the appropriate measures such as nursing, treatment, supplementary breeding and sensuality, the non-productive days of the individuals and groups of sows were improved by above measures. The study built a intuitive, fast, accurate sows early pregnancy diagnosis technology, which improved the fertility of the individuals and groups of sows increased the economic benefit of pig farm.