影响烟草特有亚硝胺积累的因素

把Ｎｉｃｏｔｉａｎａ ｐｌｕｍｂａｇｉｎｉｆｏｌｉａ的基因突变种子和普通种子发芽后嫁接在Ｗｅｓｃｏｎｓｉｎ３８烟株上,与温室条件下正常盆栽生长的Ｎｉｃｏｔｉａｎａ ｐｌｕｍｂａｇｉｎｉｆｏｌｉａ和母体Ｗｅｓｃｏｎｓｉｎ３８分析比较,试验结果得出：Ｗｅｓｃｏｎｓｉｎ３８上嫁接Ｎｉｃｏｔｉａｎａ ｐｌｕｍｂａｇｉｎｉｆｏｌｉａ基因突变种子还原酶活性最高,晾制后烟叶中烟草特有的亚硝胺含量次之;温室盆栽的     

Local orbital forcing of antarctic climate change during the last interglacial

During the last interglacial, Antarctic climate changed before that of the Northern Hemisphere. Large local changes in precession forcing could have produced this pattern if there were a rectified response in sea ice cover. Results from a coupled sea ice-ocean general circulation model supported this hypothesis when it was tested for three intervals around the last interglacial. Such a mechanism may play an important role in contributing to phase offsets between Northern and Southern Hemisphere climate change for other time intervals.     

Alterations in serotonin receptor-induced contractility of bovine lateral saphenous vein in cattle grazing endophyte-infected tall fescue

As part of a 2-yr study documenting the physiologic impact of grazing endophyte-infected tall fescue on growing cattle, 2 experiments were conducted to characterize and evaluate effects of grazing 2 levels of toxic endophyte-infected tall fescue pastures on vascular contractility and serotonin receptors. Experiment 1 examined vasoconstrictive activities of 5-hydroxytryptamine (5HT), α-methylserotonin (ME5HT; a 5HT(2) receptor agonist), d-lysergic acid (LSA), and ergovaline (ERV) on lateral saphenous veins collected from steers immediately removed from a high-endophyte-infected tall fescue pasture (HE) or a low-endophyte-infected mixed-grass (LE) pasture. Using the same pastures, Exp. 2 evaluated effects of grazing 2 levels of toxic endophyte-infected tall fescue on vasoconstrictive activities of (±)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride (DOI), BW 723C86 (BW7), CGS-12066A (CGS), and 5-carboxamidotryptamine hemiethanolate maleate (5CT), agonists for 5HT(2A),( 2B), 5HT(1B), and 5HT(7) receptors, respectively. One-half of the steers in Exp. 2 were slaughtered immediately after removal from pasture, and the other one-half were fed finishing diets for >91 d before slaughter. For Exp. 1, maximal contractile intensities were greater (P < 0.05) for steers grazing LE pastures than HE pastures for 5HT (73.3 vs. 48.9 ± 2.1%), ME5HT (52.7 vs. 24.9 ± 1.5%), and ERV (65.7 vs. 49.1 ± 2.6%). Onset of contractile response did not differ for 5HT (P = 0.26) and ERV (P = 0.93), but onset of ME5HT contraction was not initiated (P < 0.05) in HE steers until 10(-4) compared with 10(-5) M in LE-grazing steers. For Exp. 2, maximal contractile intensities achieved with DOI were 35% less (P < 0.05), whereas those achieved with 5CT were 37% greater (P < 0.05), in steers grazing HE pastures. Contractile response to CGS did not differ between pasture groups, and there was an absence of contractile response to BW7 in both groups. There were no differences between endophyte content in contractile responses after animals were finished for >91 d. Experiment 1 demonstrated that grazing of HE pastures for 89 to 105 d induces functional alterations in blood vessels, as evidenced by reduced contractile capacity and altered serotonergic receptor activity. Experiment 2 demonstrated that grazing HE pastures alters vascular responses, which may be mediated through altered serotonin receptor activities, and these alterations may be ameliorated by the removal of ergot alkaloid exposure as demonstrated by the absence of differences in finished steers.     

Losses of adult sheep due to ovine Johne's disease in 12 infected flocks over a 3-year period

OBJECTIVE: To measure the biological and financial impact of ovine Johne's disease (OJD) mortalities on 12 infected flocks within the endemic area of southern New South Wales over a 3-year period. DESIGN AND POPULATION: An observational study was conducted over a 3-year period from 2002 to 2004 on sheep from 12 OJD-infected flocks from southern NSW. Flocks ranged from between 3,500 and 20,000 sheep. At the start of the study owner estimates of OJD mortality were 5% or greater. METHOD: Annual mortality rates were estimated from farm records provided by owners. The proportion of OJD mortalities was assessed after histological examination of tissues collected from dead and moribund sheep during 5-day necropsy inspections conducted in autumn, winter, spring and summer in 2002. The financial impact was estimated using a gross margin analysis for each of the three study years and by placing a financial value on the necropsied sheep. RESULTS: On the 12 farms, the average OJD mortality rate was 6.2% (range 2.1% to 17.5%) in 2002, 7.8% (range 1.8% to 14.6%) in 2003 and 6.4% (range 2% to 11.9%) in 2004. The average decrease in gross margin due to OJD infection on a farm in 2002 was 6.4% (range 2.2% to 15.4%), 8.5% (range 3.1% to 15.8%) in 2003 and 7.4% (range 1.5% to 15.4%) in 2004. This equates to an average reduction in annual income of $13,715 per farm per year. OJD losses accounted on average for two thirds of the total estimated financial loss associated with sheep deaths. CONCLUSION: This study demonstrates the significant biological and financial impact of OJD on sheep flocks. These findings are of relevance to all Australian sheep flocks infected or at risk of OJD infection.     

Conception rates to fixed-time artificial insemination of two oestrus synchronisation programmes in dairy heifers

AIM: To evaluate the conception rate to fixed-time artificial insemination (FTAI) of two oestrus synchronisation programmes in dairy heifers on eight farms over 2 years.

METHODS: The study was conducted in 2008 and 2010 on eight farms near Palmerston North, New Zealand. Nulliparous Friesian and Friesian×Jersey heifers (13–15 months of age) were randomly allocated to one of two oestrus synchronisation programmes. Group 1 (GPG+P4; n=330), received gonadotrophin-releasing hormone (GnRH) I/M on Day 0, a progesterone (P4)-releasing intravaginal device from Days 0–7, prostaglandin F_2α (PGF) I/M on Day 7 and a second dose of GnRH at the time of FTAI on Day 9. The second group (P4+PGF; n=343) received a P4-releasing intravaginal device from Days 0–7, PGF on Day 6 and FTAI on Day 9. Pregnancy was diagnosed from Days 42–52 by transrectal ultrasonography.

RESULTS: The overall conception rate was 52.4% and 54.8% for the GPG+P4 and P4+PGF groups, respectively. The odds of conception for the two treatments were not different (OR=0.90; 95% CI=0.67–1.23), nor was there any difference between groups in different years (p=0.58). Farm affected conception rate (p=0.002), but there was no interaction with treatment (p=0.92) .

CONCLUSIONS: This study has shown that an alternative synchronisation programme can produce similar results in terms of conception rate to the GPG+P4 treatment, currently commonly used in heifers. More research is required to establish whether other modifications to the GPG+P4 programme can produce similar results at lower costs, and to identify and quantify farm factors which affect the economic benefit of heifer synchronisation.

CLINICAL RELEVANCE: This study indicated that synchronising heifers with P4 and PGF resulted in conception rates equivalent to those resulting from a GPG+P4 treatment, but with reduced drug costs. However, because heifers in the GPG+P4 group received the second GnRH injection at the time of AI, they needed only three yardings as opposed to the four required for the heifers treated with P4 and PGF. Thus, the choice of programme for an individual farm will depend on that farm's circumstances, in particular the cost of yarding the heifers.     

Antifungal agents of use in animal health--practical applications

The purpose of this paper is to provide an overview of antifungal agents currently in use in veterinary medicine. The practical applications and the therapeutic regimens that have proved successful in the treatment and prevention of fungal infections in dogs and cats, cattle and sheep, horse, pig, poultry and other birds, rodents, rabbits and fur animals are summarized.