Age prevalence of trypanosomal infections in female Glossina morsitans morsitans (Diptera: Glossinidae) on the plateau area of eastern Zambia

Trypanosomal infections in female Glossina morsitans morsitans were investigated in an area in the Eastern Province of Zambia between 1992 and 1994. A total of 4416 flies were captured, aged using the ovarian ageing method and screened for trypanosomal infections in both the mouthparts, salivary glands and the midgut. Congolense-type infections were identified in 4.8% of the flies. Vivax-type and immature infections were identified in 1.8% and 6.8% of the flies, respectively. The prevalence of congolense-type, vivax-type and immature infections increased with age. For vivax-type infections the age-prevalence relationship could be described by a model assuming a constant per capita rate of infection. For congolense-type and midgut infections, a polynomial term was added to the model significantly improving the fit. The per capita at which flies become infected was significantly higher for immature compared to mature infections. Observations strongly suggest that tsetse acquire new midgut infections at any age and that maturation of these infections is not limited to those obtained during the first blood meal.     

Managing the water balance of The Fayoum Depression,Egypt

A study of the water balances of The Fayoum irrigated lands and Lake Qarun was made to investigate the management of the irrigation system and the efficiency of irrigation water use. The two water balances are strongly interrelated. The drainage flow to Lake Qarun and the water level of the Lake are in delicate balance. A rise in Lake level causes the inundation of adjacent land. Management of The Fayoum water balance assumes control over irrigation water flows, but this control has technical and organizational limitations. Also discussed is the influence of irrigation practices in The Fayoum on the water balance (e.g., the autumn flushing of fields and farmers' preference for not irrigating at night in winter). Notwithstanding a high overall efficiency, irrigation efficiency during the winter is low. The reasons for this are given, together with the constraints against improving system management. Improved uniformity of the division and application of irrigation water will enable a better technical control of flows and will result in better water management in The Fayoum. Abbreviations: FID — Fayoum Irrigation Department, 1 feddan (fe) — 0.4 ha, 1 mcm — 1 million cubic metres: an average annual flow of 3.17 m³/s gives 100 mcm, m³/fe.year — supplied volume (m³) per surface area (fe) per year: 1000 m³/fe.year equals 240 mm/year, MSL — Mean Sea Level     

Diagnosis of regional evaporation by remote sensing to support irrigation performance assessment

The success of water management in large irrigation schemes with composites of soil, crop, wetness and micro-meteorological conditions is difficult to quantify. Performance assessment indicators, being among others a function of evaporation, are useful tools to evaluate the actual functioning of an irrigation system. The inevitable spatial variability of evaporation in large irrigation schemes makes its determination with conventional point measurements almost impossible. A new remote sensing evaporation parameterization algorithm has been tested with high resolution Landsat Thematic Mapper data for the Eastern Nile Delta, Egypt. Although the implementation of such an algorithm requires assumptions to be made, the current case study shows that these assumptions do not hamper the estimation of actual and potential evaporation at regional scale. The actual evaporation has been used to express the uniformity of crop water use which is related to the equity of irrigation water distribution. The coefficient of variance in actual evaporation between 53 differenent irrigation districts is 10% on average. The relative evaporation was considered to determine whether the crop was adequately irrigated. The relative evaporation was more than 75% for 48 out of 53 irrigation districts. It is concluded, that improved information on actual crop growth conditions through remote sensing provides an essential insight into the planning of real-time and seasonal irrigation water deliveries.     

The impact of increasing farm size and mechanization on rural income and rice production in Zhejiang province,China

Economic growth in China’s agricultural sector lags behind growth in industry and services, creating an ever widening rural–urban income gap. Development of the non-agricultural sectors offers new opportunities for farmers in China’s more advanced provinces such as Zhejiang. Increased income in the urban sector creates markets for new products, and migrating farmers rent their land to those staying. Until now, the prevailing rice-based systems have been managed mainly using manual labour and animal traction, but the larger farms resulting from migration may facilitate, or even require mechanization. In this study, we use a simulation model of the farm household to analyse the effects of increasing farm size and the transition from rice to vegetable production, while also studying the effects of mechanization.     

Using drainage systems for supplementary irrigation

The use of drainage systems for supplementary irrigation is widespread in The Netherlands. One of the operating policies is to raise the surface water level during the growing season in order to reduce drainage (water conservation) or to create subsurface irrigation. This type of operation is based on practical experience, which can be far from optimal.To obtain better founded operational water management rules a total soil water/surface water model was built. In a case study the effects of using the drainage system in a dual-purpose manner on the arable crop production were simulated with the model. Also, the operational rules for managing this type of dual-purpose drainage systems were derived.The average annual simulated increase in crop transpiration due to water conservation and water supply for subsurface irrigation are 6.0 and 5.4 mm.y^–1, respectively. This is equivalent with 520 × 10³ and 460 × 10³ Dfl.y^–1 for the pilot region (2 Dfl 1 US $). The corresponding investments and operational costs are 600 × 10³ Dfl and 9 × 10³ Dfl.y^–1 for water conservation and 3200 × 10³ Dfl and 128 × 10³ Dfl.y^–1 for subsurface irrigation. Hence, water conservation is economically very profitable, whereas subsurface irrigation is less attractive.Comparing the management according to the model with current practice in a water-board during 1983 and 1986 learned that benefits can increase with some 50 and 500 Dfl per ha per year, respectively.     

Water balance estimates of evaporation from ponded rice fields in a semi-arid region

Evaporation from ponded rice grown on fine-textured soils was calculated using a simple water balance equation, and compared with pan evaporation measured at a regional meteorological station. Crop/pan (E_r/E_p) evaporation ratios showed seasonal trends, with values generally exceeding unity during the mid- to late-season ponding period. Mid-summer ratios of E_r/E_p for two experimental fields were not significantly different from those reported in a previous study using a different experimental technique, but were higher on a third. On the average, evaporation over the whole ponding period equalled that from a Class A pan. The assumption that the infiltration or seepage term of the water balance may be neglected for fine-textured soils would seriously overestimate E_r on one of the three experimental sites.     

The Exploitation of Nematode-Responsive Plant Genes in Novel Nematode Control Methods

The molecular interactions between plants and sedentary nematodes are undergoing intense study, not only for reasons of fundamental research but also for the potential benefits to agriculture. The present technology allows the transformation of an increasing number of crop plants, providing new ways to introduce resistance against plant-parasitic nematodes. The ability of sedentary nematodes to induce specialized feeding sites in plant roots is one of the most fascinating aspects of this host–parasite interaction. Molecular approaches have been initiated to identify and characterize plant genes altered in expression after infection by sedentary nematodes. The results obtained indicate that many genes indeed become up-regulated upon nematode infection. Surprisingly, several so-called constitutive promoters that are normally used to achieve high expression in plant cells are completely ‘silenced’ in the feeding sites within days after nematode infection. Generally, there are two options available for the genetic engineering of nematode resistance: the synthesis of anti-nematode proteins or the localized production of a cytotoxic protein that interferes with the development of feeding cells. Nematode-induced promoters are very useful for the production by plants of sufficiently high levels of anti-nematode proteins at feeding sites. Alternatively, interfering with feeding-cell development is somewhat similar to the hypersensitive response evoked by nematodes in a naturally resistant plant. Here, destruction of specific plant cells can be achieved by the localized expression of a cytotoxin such as barnase, a potent ribonuclease. This approach, however, calls for a highly specific ‘non-leaky’ promoter, which is active only in the feeding cells. Another possibility is to use a two-component system, where the leakiness of the promoter in other tissues is counterbalanced by the constitutive expression of a neutralizing gene.     

Potential chemical time bombs in the schelde estuary

The Schelde estuary is heavily polluted with many different (micro)pollutants. This results in high concentrations in the abiotic and biotic compartments of the Schelde estuary and in various effects. the present day suboxic and anoxic conditions in the upper estuary are probably responsible for the high distribution coefficients for some trace metals in the lower Schelde estuary (Western Schelde). a future reduction in discharges with a high biological oxygen demand will therefore not only result in higher oxygen concentrations, but probably also in lower distribution coefficients for trace metals in the Western Schelde. Simulations show that lower trace metal distribution coefficients will probably result in higher concentrations of dissolved (bioavailable) trace metals, even with substantially reduced discharge rates, due to the desorption of trace metals from resuspended bottom sediments.     

Marine-Derived Chitosan Nanoparticles Improved the Intestinal Histo-Morphometrical Features in Association with the Health and Immune Response of Grey Mullet (Liza ramada)

Marine-derived substances are known for their beneficial influences on aquatic animals’ performances and are recommended to improve intestinal health, immunity, and anti-oxidative status. The present study investigates the role of chitosan nanoparticles on the intestinal histo-morphometrical features in association with the health and immune response of Grey Mullet (Liza ramada). Chitosan nanoparticles are included in the diets at 0, 0.5, 1, and 2 g/kg and introduced to fish in a successive feeding trial for eight weeks. The final body weight (FBW), weight gain (WG), and specific growth rate (SGR) parameters are significantly increased while feed conversion ratio (FCR) decreases by chitosan nanoparticles compared to the control (p < 0.05). The morphometric analysis of the intestines reveals a significant improvement in villus height, villus width, and the number of goblet cells in chitosan-treated groups in a dose-dependent manner. Additionally, there is a positive correlation between the thickness of the enterocyte brush border and the chitosan dose, referring to an increasing absorptive activity. Histologically, the intestinal wall of Grey Mullet consists of four layers; mucosa, sub-mucosa, tunica muscularis (muscular layers), and serosa. The histological examination of the L. ramada intestine shows a normal histo-morphology. The epithelial layer of intestinal mucosa is thrown into elongated finger-like projections, the intestinal villi. The values of hemoglobin, hematocrit, red blood cells (RBCs), total protein (TP), albumin, and globulin are significantly increased in fish fed 1, and 2 g/kg of chitosan nanoparticles compared to fish fed 0 and 0.5 g/kg (p < 0.05). The highest levels of TP and albumin are observed in fish fed 1 g/kg diet (p < 0.05). The lysozyme activity and phagocytic index are significantly enhanced by feeding chitosan nanoparticles at 0.5, 1, and 2 g/kg, whereas the phagocytic activity is improved in fish fed 1 and 2 g/kg (p < 0.05). The highest lysozyme activity and phagocytic index are observed in fish fed 1 g/kg. SOD is significantly activated by feeding chitosan nanoparticles at 1 g/kg. Simultaneously, glutathione peroxidase (GPx) and catalase (CAT) activities also are enhanced by feeding chitosan at 1 and 2 g/kg, compared to fish fed 0 and 0.5 g/kg (p < 0.05). The highest GPx and CAT activities are observed in fish fed 1 g/kg (p < 0.05). Conversely, the malondialdehyde (MDA) levels are decreased by feeding chitosan at 1 and 2 g/kg, with the lowest being in fish fed 1 g/kg (p < 0.05). To summarize, the results elucidate that L. ramada fed dietary chitosan nanoparticles have a marked growth rate, immune response, and anti-oxidative response. These improvements are attributed to the potential role of chitosan nanoparticles in enhancing intestinal histo-morphometry and intestinal health. These results soundly support the possibility of using chitosan nanoparticles at 1–2 g/kg as a feasible functional supplement for aquatic animals.