全文获取类型
收费全文 | 70847篇 |
免费 | 4045篇 |
国内免费 | 34篇 |
专业分类
林业 | 3235篇 |
农学 | 2529篇 |
基础科学 | 477篇 |
8719篇 | |
综合类 | 11417篇 |
农作物 | 2617篇 |
水产渔业 | 3640篇 |
畜牧兽医 | 36618篇 |
园艺 | 907篇 |
植物保护 | 4767篇 |
出版年
2018年 | 1057篇 |
2017年 | 1130篇 |
2016年 | 1057篇 |
2015年 | 911篇 |
2014年 | 1142篇 |
2013年 | 2631篇 |
2012年 | 2063篇 |
2011年 | 2464篇 |
2010年 | 1684篇 |
2009年 | 1751篇 |
2008年 | 2600篇 |
2007年 | 2364篇 |
2006年 | 2330篇 |
2005年 | 2129篇 |
2004年 | 2194篇 |
2003年 | 2167篇 |
2002年 | 2039篇 |
2001年 | 2366篇 |
2000年 | 2375篇 |
1999年 | 1925篇 |
1998年 | 912篇 |
1997年 | 821篇 |
1996年 | 782篇 |
1995年 | 861篇 |
1994年 | 843篇 |
1993年 | 767篇 |
1992年 | 1523篇 |
1991年 | 1542篇 |
1990年 | 1663篇 |
1989年 | 1507篇 |
1988年 | 1451篇 |
1987年 | 1340篇 |
1986年 | 1384篇 |
1985年 | 1336篇 |
1984年 | 1129篇 |
1983年 | 1025篇 |
1982年 | 628篇 |
1979年 | 1004篇 |
1978年 | 795篇 |
1977年 | 717篇 |
1976年 | 676篇 |
1975年 | 719篇 |
1974年 | 766篇 |
1973年 | 830篇 |
1972年 | 803篇 |
1971年 | 748篇 |
1970年 | 729篇 |
1969年 | 744篇 |
1967年 | 658篇 |
1966年 | 631篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
101.
A W vd Giessen R Peters P A Berkers W H Jansen S H Notermans 《The Veterinary quarterly》1991,13(1):41-46
The contamination of poultry in the Netherlands with Salmonella enteritidis was tested. For this, different methods (detection of S. enteritidis in faecal samples of 25 g; detection of S. enteritidis in cloacal swabs; detection of S. enteritidis by serological testing of antibodies in serum) were compared for their efficiency to detect S. enteritidis in flocks of poultry. Testing of faecal samples clearly yielded the best results. This method was used in a transmission study, in which 14 flocks descending from a contaminated primary mother flock were screened for the presence of S. enteritidis. The method was also used for screening 49 flocks of laying hens and 52 flocks of broiler chickens throughout the Netherlands. From the transmission study it became clear that S. enteritidis, phage type 2 (Dutch phage set) was isolated both from the mother flock and from five of the descendent flocks. Screening of poultry flocks for the presence of salmonella revealed that salmonella was present in 47% of the layer flocks and in 94% of the broiler flocks. S. enteritidis was isolated from 15% of the flocks screened. 相似文献
102.
A P Davidson 《Veterinary Clinics of North America: Small Animal Practice》2001,31(2):411-420
The practice of small animal theriogenology is rewarding, but frustrations exist concerning technologic advances as compared with other species. Reproductive clinicians striving to practice good quality medicine readily identify topics of common concern: causes that are not identified or therapeutics that are not available or applicable. Improved collaboration among theriogenologists specializing in small animal practice is evidenced by growing attendance at national and international scientific meetings, increased scientific publications, and internet communications. 相似文献
103.
An ammonium sulfate fraction of Taenia hydatigena cyst fluid (ThFAS) was further fractionated by hydrophobic interaction chromatography, using alkylagarose and omega-amino alkylagarose columns, in an effort to isolate and purify a specific diagnostic antigen in the ThFAS preparation. The less than 12 kDa antigen was found to have an affinity for immobilized alkanes with chain length of six carbons or greater. The antigen was recovered in an ethylene glycol eluate from a hexylagarose column then analyzed by Western blot; it reacted with bovine and human cysticercosis infection sera and with specific monoclonal antibodies but not with control sera or fascioliasis infection sera. When the eluate was used as coating antigen in a plate ELISA assay no false positive reactions were seen in sera from cattle infected with Fasciola hepatica; false positive reactions were observed for the unfractionated ThFAS antigen preparation. 相似文献
104.
K.A. Jarvis Ph.D M.J. Woliner BS E.P. Steffey VMD PhD 《Veterinary anaesthesia and analgesia》1992,19(1):41-45
The accuracy of thermodilution for measuring flow rates of 10–40 L/min was evaluated using a commercially available thermodilution cardiac output computer in an in vitro model. Water (36.5–37.5°C) was directed through a mixing chamber via a constant flow pump. Thermodilution estimates of flow using four different volumes (10, 20, 30, 40 ml) of iced water injectate were compared to simultaneous measurements of timed samples of effluent from the mixing chamber. Injectate volume had a significant impact on the accuracy of thermodilution estimation (p < 0.05). Thermodilution overestimated measured flow when 10 and 20 ml of injectate were used to determine flow rates < 20 L/min but underestimated flow when injectate volumes of 30 and 40 ml were used, or when measured flow was > 25 L/min. The discrepancy between thermodilution flow and measured flow increased as rate of fluid flow increased. 相似文献
105.
Thyroid scintigraphy in small animals 总被引:1,自引:0,他引:1
106.
107.
D. Sharifi J. Singh P.K. Peshin A.P. Singh 《Veterinary anaesthesia and analgesia》1991,18(Z1):309-314
Cardio pulmonary embarrassment was induced with thiopentone sodium in 35 healthy adult donkeys divided in to seven groups of five animals. “To effect” anaesthetic dose was (12.72 ± 0.69 mg/kg) which caused transient hypotension, tachycardia and hypoxemia. A stable fall in MAP 1. 6.77 ± 0.287 Kpa (50.78 ± 2.15 mmHg) and a flat EEG were considered to indicate acute circulatory insufficiency due to thiopentone overdose. 相似文献
108.
C. P. Verschueren P. J. Selman J. J. M. de Vijlder J. A. Mol 《Domestic animal endocrinology》1991,8(4):509-519
Canine thyroglobulin (cTg) has been isolated and purified. It has similar electrophoretic patterns as Tg from other mammalian species. The main fraction had a MW of 660,000, whereas also fractions of a MW of approximately 1,300,000 (dimer) and 330,000 (subunit) were present. The iodine content was 0.8 to 1.0 % (w/w). cTg did not cross-react with antibodies against human Tg to a degree that would allow the use of a radioimmunoassay for human Tg for the determination of cTg in serum or plasma. Therefore a polyclonal antiserum was raised against cTg and a homologous radioimmunoassay was developed, which was sensitive (0.4 μg/l) and specific (cross-reactivity in cTg assay of human Tg, goat Tg, T4, T3, and DIT < 0.01 %).
Plasma Tg levels in normal dogs of both sexes and aged 3–15 years amounted to 192 ± 73 μg/l (mean ± SD, n=30). There was no relation between plasma Tg and T4 levels. 相似文献
109.
荷兰是全世界畜牧业高度发达的国家之一。养猪业也象奶牛业一样发展很好,特别是猪场管理很值得我国养猪同行学习。该国的养猪规模很大,一般都在2000头左右。其养猪业的先进性在于:年头均产胎指数2.3,年头均母猪出栏仔猪23头,胎产活仔数11.8头,受胎率85%,仔猪早期断奶及母猪年更新率为50%。 相似文献
110.
J.A. Díez P. Hernaiz M.J. Muñoz A. de la Torre A. Vallejo 《Soil Use and Management》2004,20(4):444-450
Abstract. The repeated application of pig slurry to agricultural soils may result in an accumulation of salts and a risk of aquifer pollution due to nitrate leaching and salinization. Under Mediterranean conditions, a field experiment on a sandy loam soil (Typic Xerofluvent) was performed with maize (Zea mays) in 1998, 1999 and 2001 to study the effects of applying optimal (P1) and excessive rates (P3) of pig slurry on soil salinization, nitrate leaching and groundwater pollution. The rate of pig slurry was established considering the optimal N rate for maize in this soil (170, 162 and 176 kg N ha?1 for 1998, 1999 and 2001, respectively). Pig slurry treatments were compared to an optimal N rate supplied as urea (U) and a control treatment without N fertilizer (P0). The composition of the slurries showed great variability between years. Mean NO3? leaching losses from 1998 to 2001 were 329, 215, 173 and 78 kg N ha?1 for P3, P1, U and P0 treatments, respectively. The amount of total dissolved salts (TDS) added to the soil in slurry application between 1998 and 2001 was 2019 kg TDS ha?1 for the P1 treatment and 6058 kg TDS ha?1 for the P3 treatment. As a consequence, the electrical conductivity (EC) of the slurry‐treated soils was greater than that of the control soil. The EC correlated significantly with the sodium concentration of the soil solution. Over the entire experimental period, 2653, 2202 and 2110 kg Na ha?1 entered the aquifer from the P3, P1 and P0 treatments, respectively. The P3 treatment did not significantly increase grain production in 1999 and 2001 compared with that achieved with the optimal N rate treatment (P1). This behaviour shows the importance of establishing application guidelines for pig slurry that will reduce the risk of soil and groundwater pollution. 相似文献