Monoclonal antibodies reacting specifically with Francisella sp

Twenty two hybridoma strains producing monoclonal antibodies against Francisella tularensis ATCC 6223, var. tularensis, were characterized. In an enzyme-linked-immunosorbent-assay (ELISA) using formaldehyde fixed bacteria as antigens, neither cross-reactions with six different Brucella spp., with Yersinia enterocolitica 0:9 nor with two biotypes of Yersinia pseudotuberculosis could be detected. The antibodies gave comparable titres with the three strains of F.tularensis tested. ELISA binding studies indicated that fifteen of the antibodies bound with high affinities to their epitopes of the three Francisella strains, while the others each seemed to bind with low affinity to at least one of the antigens. Immunoblot analysis showed that six of the antibodies were directed to epitopes on the core moiety of the lipopolysaccharide molecule, while the other 16 antibodies bound to O side chain components.     

Effect of clear-felling on runoff in two small watersheds in Central Sweden

Small watersheds were used in a study of the effect of clear-felling on runoff from coniferous forest areas in Central Sweden. After a 3-year calibration period, two watersheds were clear-felled (on one of them the slash was left and on the other slash was removed) and a third was left as a reference.Runoff under forested conditions was 271 and 246 mm year^?1, respectively for the treated watersheds, while the precipitation measured 732 mm year^?1 as a mean for the investigated period. Clear-felling resulted in an increase in runoff of 119 and 75% (371 and 215 mm per year), respectively. The increase occurred during the period of snowmelt (April–May) and the period without snow (June–October). During winter (November–March) a very small increase in runoff was noted. The effect of clear-felling on runoff appears to be greater than has been reported from most other Scandinavian investigations.The increase in runoff during snowmelt is probably due to an increased snow accumulation in the clear-felled area as compared to the forest. The increased runoff for the period without snow is explained by reduced transpiration from the vegetation and reduced evaporation caused by an increased aerodynamic resistance.No effects of slash removal could be detected, probably because of the natural variation between the two treated watersheds.     

Survey of vomitoxin-contaminated feed grains in midwestern United States, and associated health problems in swine

During the 1981 corn harvest season in Illinois and surrounding states, cold wet weather enhanced the growth of Fusarium graminearum, with resulting contamination by vomitoxin and, to a lesser extent, zearalenone. Of 342 feed samples analyzed, 274 contained vomitoxin at a concentration ranging from 0.1 to 41.6 ppm (mean, 3.1 ppm) and 40 samples contained zearalenone at a concentration ranging from 0.1 to 8 ppm (mean, 0.66 ppm). Animal health problems and reduced growth performance were observed mainly in swine fed vomitoxin-contaminated rations. The predominant clinical complaints, in decreasing frequency were: reproductive problems (50%), feed refusal (43%), reduced weight gain (25%), diarrhea (17%), death (14%), and emesis (11%).     

An ELISA with Brucella lipopolysaccharide antigen for the diagnosis of B. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival B. melitensis strain Rev 1 vaccination.

An indirect enzyme-linked immunosorbent assay (ELISA) with unpurified Brucella melitensis smooth lipopolysaccharide (S-LPS) as antigen was evaluated for the serological diagnosis of B. melitensis infection in sheep in comparison with the Rose Bengal (RB), complement fixation (CF), radial immunodiffusion (RID), microplate agglutination (MA) and rivanol agglutination (RIV) tests. Tests RB and CF detected as positive each of the 77 sera from B. melitensis-infected animals tested, the RID (74), MA (76) and the RIV (72) were less sensitive. However, all tests compared were negative when 77 sera from Brucella-free rams were tested. While subcutaneous Rev 1 vaccination induced high response levels in any of the tests, low level responses were obtained upon conjunctival vaccination, particularly in ELISA and RID tests.