Studies on the suitability of trap-tubs to control noxious rodents in forests

Various types of trap-tubs were tested both under controlled conditions in standardised trial series and in the field. New trap-tubs were poorly accepted by the animals, whereas weathered tubs were readily accepted within a very short period of time. The trapping mechanisms of tubs used in Lower Saxony and improved tubs in Hesse were both found to be suitable. The trapping method proved to be highly selective since long-tailed mice (Apodemus spp.) can easily escape by jumping onto the edge of the tub. Voles (Microtus agrestis, Clethrionomys glareolus) explored the inside of the tub and worked its exits with decreasing intensity during the time of captivity. They died of hypothermia within a few hours. Elimination of the trapped rodents by their natural enemies was not observed. The trials showed that trap-tubs are suitable for selective reduction of the density of field voles and red-backed voles in forests. The results also contribute to assessing the humaneness of the control method.     

Absorption of inorganic, trivalent and hexavalent chromium following oral and intrajejunal doses in rats

The intestinal absorption of trivalent and hexavalent chromium (Cr) given orally (experiment I) or infused in the intestine (experiment II) was investigated in rats. The nonabsorbable form of chromium (51Cr2O3) and water-soluble and more absorbable Na2(51)CrO4 (the hexavalent form of Cr) were compared. Total retention of chromium given orally ranged around 15 percent of the dose, regardless of the chromium compounds applied. The absorption rate of chromic oxide, which is considered a nonabsorbable compound, was 14.4 as a percentage of chromium intake. This result indicates that some loss of chromium has to be taken into account in metabolic trials made by the indicator method. In isolated rat intestine, from the injected Cr 2.5% of chromic oxide and 43.2% of sodium chromate were absorbed during an hour (experiment II). The absorbed chromium was transferred to the liver where the liver tissue retained 10.9% of chromic oxide and 51.1% of sodium chromate. Radioactivity of v. cava caudalis following intestinal injection of Na2CrO4 was thirtyfold greater than after Cr2O3 dosing. This phenomenon can be explained by the lower blood clearance of chromate. Different absorption rate of chromate depending on the route of administration could be due to the fact that the hexavalent form given orally was reduced to Cr3+ in the acidic environment of the stomach. When Na2CrO4 was infused directly in the intestine of rats, such reduction could not occur. This means that the acidic gastric juice might play a role in inhibiting the intestinal absorption of Na2CrO4 when this compound is given orally.     

What proteomic analysis of the apoplast tells us about plant–pathogen interactions

Plant pathogens have developed different strategies during their evolution to infect and colonize their hosts. In the same way, plants have evolved different mechanisms acting against potential pathogens trying to infect and colonize their tissues. Regulation of a wide variety of proteins is required in order to perceive the pathogen and to activate the plant defence mechanisms. The apoplast is the first compartment where these recognition phenomena occur in most plant–pathogen interactions, allowing the exchange of different molecules and facilitating inter‐ and intracellular communication in plant cells. Proteomic analysis of the apoplast in recent years has found the initial biochemical responses involved in pathogen recognition and early defence responses. However, this proteomic approach requires some specific experimental conditions to obtain an extract free of cytoplasmic proteins and nonprotein contaminants that affect the subsequent stages of separation and quantification. Obtaining the highest proportion of proteins from the apoplastic space in infected tissues requires different steps such as extraction of apoplastic washing fluids and preparation of total secreted proteins (protein precipitation, solubilization, separation and digestion). Protein identification using mass spectrometry techniques and bioinformatics tools identifying peptides for the extracellular exportation is required to confirm the apoplastic location. This review compiles the most commonly used techniques for proteomic studies, focusing on the early biochemical changes occurring in the apoplast of plants infected by a wide range of pathogens. The scope of this approach to discover the molecular mechanisms involved in the plant–pathogen interaction is discussed.     

Coupled ferric oxides and sulfates on the Martian surface

The Mars Exploration Rover (MER), Opportunity, showed that layered sulfate deposits in Meridiani Planum formed during a period of rising acidic ground water. Crystalline hematite spherules formed in the deposits as a consequence of aqueous alteration and were concentrated on the surface as a lag deposit as wind eroded the softer sulfate rocks. On the basis of Mars Express Observatoire pour la Minéralogie, l'Eau, les Glaces et l'Activité (OMEGA) orbital data, we demonstrate that crystalline hematite deposits are associated with layered sulfates in other areas on Mars, implying that Meridiani-like ground water systems were indeed widespread and representative of an extensive acid sulfate aqueous system.     

不同维生素预混料对科宝肉鸡生长性能、胫骨质量和抗氧化能力的影响

试验研究3种维生素预混料(处理1、2、3)对科宝肉鸡生长性能、胫骨质量及抗氧化能力的影响。1 200只科宝肉鸡随机分为3个处理,每个处理10个重复,每个重复40只鸡,试验期42 d。结果表明:与处理1相比,处理3和2可显著提高肉鸡21 d体重,显著降低1～21 d饲料增重比;处理3还显著提高肉鸡1～21 d体增重、采食量和21 d胫骨灰分和磷比例及42 d胫骨灰分、钙比例(P<0.05);与处理2相比,处理3显著提高肉鸡42 d胫骨磷比例(P<0.05)。与处理2和1相比,处理3显著提高肉鸡21 d血清总超氧化物歧化酶(T-SOD)活力(P<0.05)。结果表明,根据科宝肉鸡的生长性能、胫骨质量和机体抗氧化能力,适宜维生素预混料为处理3。     

Effect of human disturbance on the structure and regeneration of forests in the Nevado de Toluca National Park, Mexico

Sample plots were established in the principal forest types in the the Nevado de Toluca National Park, Mexico including those dominated by Pinus hartwegii, Abies religiosa, Quercus laurina and Alnus jorullensis. The vertical structure was defined by three strata in the coniferous forests and two strata in the broadleaved forests. Timber harvesting in Abies religiosa and Quercus laurina forests and fires generated by humans in Pinus hartwegii forests impeded the recruitment of saplings. Mature trees were also heavily impacted by logging in Pinus hartwegii forests. On the contrary, Alnus jorullensis forests were increasing due to the disturbance of Pinus and Quercus forests, as well abandoned crop lands within the park. A combination of logging, uncontrolled fire, and grazing appears to be compromising the recruitment of important tree species in this national park. These factors, together with human settlements, have also increased the proportion of early successional species. Changes in forest structure from human disturbance indicate a need to control these activities if conservation goals are not to be compromised.     

Species distribution models applied to plant species selection in forest restoration: are model predictions comparable to expert opinion?

An expert on local flora usually is the best option for plant species selection in most ecological restoration projects; although species selection often needs to be dealt with swiftly as well as on a limited budget, and obtaining the opinion of a local expert may not always be an economically viable alternative. In such cases, species distribution models (SDM) may offer a faster and more cost effective alternative. We asked six experts to rank native tree species according to their suitability at 24 forest sites. The predictive performance of the suitability rankings was evaluated by assessing their ability to discriminate present from absent species in the observed tree assemblages at each evaluation site. We used the area under the receiver operating characteristic curve to calculate the probability that the estimated suitability for a species present at a particular evaluation site is greater than the estimate for an absent species (both picked at random). Suitability rankings were also obtained from the predictions of SDM and the same procedure was used to estimate the predictive performance of the set of models at each site. The experts offered concordant suitability rankings at almost every evaluation site. There were no significant differences in the predictive performance of the SDM and four of the experts, although the SDM performed slightly better than the other two experts. Our results point to the suitability of the proposed species distribution modeling approach to obtain fast and cost effective recommendations for species selection in forest restoration projects.     

Clinical remission following plasmapheresis and corticosteroid treatment in a dog with acquired myasthenia gravis

A 7-year-old sexually intact male Labrador Retriever with regurgitation and generalized muscular weakness resulting from acquired myasthenia gravis received 2 plasmapheresis treatments in combination with corticosteroid treatment. Plasmapheresis was performed in an attempt to rapidly lower serum acetylcholine receptor binding antibody (AChR Ab) concentration. Seven days after the second plasmapheresis treatment, the dog's muscular strength was normal, which coincided with a 70% decrease in serum AChR Ab concentration. Because the dog also received corticosteroids, it is impossible to determine how much of the clinical improvement resulted from plasmapheresis.     

A method for establishing a core collection of Saccharum officinarum L. germplasm based on quantitative-morphological data

A technique is proposed for establishing a representative core collection of S. officinarum accessions from the world collection of sugarcane germplasm maintained at the Sugarcane Breeding Institute-Research Centre, Cannanore, India. In the proposed method, the accessions were first sorted based on their relative contributions to the total variability by means of principal component scores based on a set of quantitative characters. Then, the cumulative proportion of their contributions to the total variance was computed. A logistic regression model was fitted to evaluate the functional relationship between the cumulative proportion of variance and the number of accessions. The size of the core set was decided as the inflection point on that fitted curve, i.e., the point beyond which the rate of increase in cumulative proportion of variability contributed by an accession began to decline. A method for eliminating entries with a high degree of similarity from the selected core set is also proposed.