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41.
J Przala T Kaminski S Okrasa G Siawrys & I Bogacka 《Reproduction in domestic animals》2001,36(2):107-112
The amount of β‐endorphin‐like immunoreactivity (β‐END‐LI) in porcine corpora lutea from several stages of the oestrous cycle and the effects of progesterone, oxytocin, and prolactin on β‐END‐LI secretion in vitro by luteal cells were studied. Porcine corpora lutea obtained on days 1–5, 6–10, 11–13, 14–18, and 19–21 of the cycle were used to prepare extracts for β‐END‐LI determination. Additionally, corpora lutea from days 11–13 and 14–18 were enzymatically dissociated and isolated luteal cells were used for further study of β‐endorphin secretion in vitro. Cells were cultured in serum‐free defined M 199 medium (106 cells/ml) at 37°C under 5% CO2 in air, for 12 h. The influences of the following factors on β‐END‐LI secretion by luteal cells were tested: progesterone (10–9, 10–7 and 10–5M ), oxytocin (0.01, 0.1, 1 and 10 ng/ml), and prolactin (0.1, 1, 10 and 100 ng/ml). The β‐END‐LI contents in extracts and media were measured by radioimmunoassay. The tissue concentration of β‐END‐LI was lowest on days 1–5 of the cycle (0.35 ± 0.03 ng/g wet tissue). Subsequently, it constantly increased to the highest value on days 14–18 (16.58 ± 0.52 ng/g wet tissue) and on days 19–21 it declined (11.10 ± 0.52 ng/g wet tissue). Progesterone at a low dose (10–9 M ) resulted in significant (p < 0.05) increases and decreases in β‐END‐LI secretion by luteal cells from days 11–13 and 14–18, respectively. Higher doses of progesterone (10–7 and 10–5 M ) had no effect on β‐END‐LI release, compared with the control group. All dose‐levels of oxytocin used decreased β‐END‐LI secretion by luteal cells on days 11–13 and 14–18 of the cycle. Prolactin at doses of 0.1 and 1 ng/ml on days 11–13, and all doses tested on days 14–18 resulted in decreases in β‐END‐LI release from luteal cells. These results document evident changes in β‐END‐LI content in the pig corpus luteum during its development and indicate the potential roles of progesterone, oxytocin, and prolactin in luteal cell secretion of β‐END‐LI. 相似文献
42.
Abstract. Recent developments in in situγ ray spectrometry offer a new approach to measuring the activity of radionuclides such as 137Cs and 40K in soils, and thus estimating erosion or deposition rates and field moist bulk density (ρm). Such estimates would be rapid and involve minimal site disturbance, especially important where archaeological remains are present. This paper presents the results of a pilot investigation of an eroded field in Scotland in which a portable hyper pure germanium (HPGe) detector was used to measure γ ray spectra in situ. The gamma (γ) photon flux observed at the soil surface is a function of the 137Cs inventory, its depth distribution characteristics and ρm. A coefficient, QCs, derived from the forward scattering of 137Cs γ ray photons within the soil profile relative to the 137Cs full energy peak (662 keV), was used to correct the in situ calibration for changes in the 137Cs vertical distribution in the ploughed field, a function of tillage, soil accumulation and ρm. Based on only 8 measurements, the agreement between in situγ ray spectrometry and soil sample measurements of 137Cs inventories improved from a non significant r2=0.05 to a significant r2=0.62 (P<0.05). Erosion and deposition rates calculated from the corrected in situ137Cs measurements had a similarly good agreement with those calculated from soil cores. Mean soil bulk density was also calculated using a separate coefficient, QK, derived from the forward scattering γ photons from 40K within the soil relative to the 40K full energy peak (1460 keV). Again there was good agreement with soil core measurements (r2=0.64; P<0.05). The precision of the in situ137Cs measurement was limited by the precision with which QCs can be estimated, a function of the low 137Cs deposition levels associated with the weapons testing fallout and relatively low detector efficiency (35%). In contrast, the precision of the in situ ρm determination was only limited by the spatial variability associated with soil sampling. 相似文献
43.
Two cases of H.c.c. which occurred in winter 1987 in Vienna are described. Case one was a female Chow-Chow, 8 weeks of age, that died from the peracute form of the disease. The diagnosis was confirmed by histology and direct immunofluorescence. Case two, a 9-month old female Kuvacz, showed clinical signs of the subacute form of H.c.c. She was hospitalized and therapy was successful. The disease was diagnosed by the typical clinical signs and the raise of antibodies in paired serum samples. Etiology, clinical signs and immunology of H.c.c. are discussed. 相似文献
44.
Bilateral nephrolithiasis with intermittent ureterolithiasis was diagnosed in a 7-year old Holstein cow. Two episodes of ureterolithiasis resulted in severe azotemia which resolved after spontaneous movement of the stone. A third episode of obstruction one year after the initial episode resulted in rupture of one kidney, necessitating euthanasia. The histopathological examination of the kidney was diagnostic for chronic pyelonephritis. Corynebacterium sp. was cultured from a nephrolith. In this case it is believed that the chronic pyelonephritis predisposed to the calculi formation. 相似文献
45.
Effect of a new pelleting process on the level of contamination of poultry mash by Escherichia coli and Salmonella 总被引:2,自引:0,他引:2
R H McCapes H E Ekperigin W J Cameron W L Ritchie J Slagter V Stangeland K V Nagaraja 《Avian diseases》1989,33(1):103-111
The efficacy of a new pelleting process in eliminating naturally occurring Escherichia coli and salmonella from poultry mash was assessed by comparing the microbial load in raw and processed mash. Instead of using steam produced in a boiler, the new process conditioned mash in an Original Vertical Conditioner with steam and other hot gases generated by direct combustion in a Vaporator. E. coli was isolated from 72-100% of samples of raw mash in all trials, and the mean number of colony-forming units of E. coli/g of samples was 6.8 +/- 4.0 X 10(4). Salmonellae (S. senftenberg, S. bredeney, and S. mbandaka) were isolated from 5-10% of samples of raw mash utilized in three of the seven trials. Within limitations of the sampling and analytical tests utilized, the new pelleting process eliminated salmonella from all mash in which the organism was known to be present but eliminated E. coli in only three trials. The process appeared to be 100% effective against both organisms when mash entering the pellet mill from the conditioner had a temperature of 85.7 C and a moisture content of 14.5% and had been retained and treated with steam and hot gases for 4.1 minutes. 相似文献
46.
Y Zhou V Moennig C O Coulibaly J Dahle B Liess 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(1):76-80
Monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. The animals had been infected simultaneously with both viruses. The antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. A polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. Both techniques based on monoclonal antibodies were more sensitive and more specific than the conventional test, the enzyme immunoassay being more sensitive than the immunofluorescence test. Small amounts of BVD viral antigen were demonstrable with monoclonal antibodies in most organ tissues. 相似文献
47.
Duration of maternal immunity to peste des petits ruminants 总被引:1,自引:0,他引:1
48.
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50.
The state of mucus synthesis in the goblet cells of the small intestine was studied in conventional piglets infected with a dose of 200,000 oocytes of the coccidium Isospora suis the first and fifth day after parturition. The synthesis of mucus and its chemical characteristics undergo significant changes during the third and fourth day after infection. The activity of acid and neutral mucous substances declines; their level and the physiological synthetic function of goblet cells begin to return to the normal during the period starting on the eight to tenth day after infection. However, there were no fully functioning goblet cells in the broken numerical ratio even at the end of the period of investigation, i.e. the 13th day after infection. The thin surface layer of mucus remained almost unchanged within the whole extent of the small intestine parts studied. 相似文献