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排序方式: 共有367条查询结果,搜索用时 15 毫秒
201.
202.
Akifumi OHTANI Masahito KUBO Hiroshi SHIMODA Kenji OHYA Tadashi IRIBE Daiki OHISHI Daiji ENDOH Tsutomu OMATSU Tetsuya MIZUTANI Hideto FUKUSHI Ken MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):777-782
Chlamydia pecorum (designated 22–58) was isolated in 2010 in
HmLu-1 cells from the jejunum of a calf which died of necrotizing enterocolitis in
Yamaguchi Prefecture, Japan. Immunohistochemical staining identified C.
pecorum positive reactions in the jejunal villi. C. pecorum,
designated 24–100, was isolated from the feces of a calf with diarrhea in another farm in
Yamaguchi Prefecture in 2012. A significant increase in neutralizing antibody titers
against C. pecorum was confirmed in paired sera. Nucleotide sequence
identities of omp1 genes of the 2 isolates were 100%. The isolates were
genetically and antigenically more closely related to C. pecorum
Bo/Yokohama strain isolated from cattle with enteritis in Japan than to the other
prototype strains, Bo/Maeda isolated from cattle with pneumonia and Ov/IPA isolated from
sheep with polyarthritis. These results indicate that C. pecorum strains
similar to 22–58 and 24–100 might be endemic in Yamaguchi Prefecture and cause enteric
disease in cattle. 相似文献
203.
Okamoto M Oku Y Kurosawa T Kamiya M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(2):159-163
DNA from several isolates of Taenia taeniaeformis and Echinococcus multilocularis were digested with restriction enzymes and hybridized with digoxigenated oligonucleotide probe (CAC)5. Within the six wild isolates of Taenia taeniaeformis from Norway rats in Hokkaido, although several bands were common among isolates, fingerprinting patterns were specific to each isolate. In the case of E. multilocularis, regardless of hosts from which each isolate has been isolated, the five isolates collected from Hokkaido, showed the same fingerprinting pattern. These results indicate that there was very little genetic difference among these isolates. Although the fingerprinting pattern of E. multilocularis from St. Lawrence Is. was similar to that of the Hokkaido isolates, some bands were different from those in the Hokkaido isolates. Echinococcus multilocularis in Hokkaido seems to be closely-related genetically to that from St. Lawrence Is. 相似文献
204.
Kaneshima T Hohdatsu T Hagino R Hosoya S Nojiri Y Murata M Takano T Tanabe M Tsunemitsu H Koyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(3):301-303
Canine respiratory coronavirus (CRCoV), which is more closely related to the bovine coronavirus (BCoV), has recently been detected in dogs. In this study, we examined whether BCoV was capable of infecting and exhibiting pathogenicity in dogs. Three 1-month-old pups were oronasally given field isolates of BCoV, and were kept together with 2 control animals. As a result, increases in BCoV-neutralizing antibody titers were confirmed in all pups in the challenged and control groups. Moreover, the virus gene was also detected in oral and rectal swabs by RT-PCR. These results indicate that BCoV infects dogs, and easily infects other dogs that are kept together. However, no clinical symptoms such as respiratory symptoms and diarrhea were observed. 相似文献
205.
Tsutomu Arie Yumiko Kobayashi Yoshiki Kono Okada Gen Isamu Yamaguchi 《Pest management science》1999,55(5):602-604
Phoma glomerata strain JCM9972 controls clubroot of cruciferous crops caused by Plasmodiophora brassicae and the activity depends upon epoxydon (5-hydroxy-3-(hydroxymethyl)-7-oxabicyclo [4.1.0]hept-3-en-2-one) produced by the strain. 相似文献
206.
Tetsuji Itoh Yoshikazu Sato Tsutomu Mano Ryûtarô Iwata 《Journal of Forest Research》2009,14(2):117-122
We examined 24 microsatellite markers to
select those most suitable for individual identification
and parentage tests of brown bears
(Ursus arctos)
based on statistical parameters and experimental error
using liver samples (38 issues) from nuisance bears killed
in the Akan–Shiranuka Region, eastern Hokkaido, Japan from
1996 to 2006. We found seven microsatellite markers suitable
for identifying individuals (G1A, G10B, G10L, UarMU5, UarMU23,
UarMU50, and UarMU51; probability of identity
P
id = 3.17
× 10−7; probability of identity of
sibs P
id-sib
= 2.23 × 10−3), 12 microsatellite
markers suitable for parentage testing when one parent is
known [G1A, G1D, G10B, G10L, G10P, UarMU5, UarMU23, UarMU50, UarMU51,
UarMU59, UarMU61, and UarMU64; probability of excluding one parent
(PE I = 0.9991)], and 15 microsatellite markers for parentage
testing when neither parents are known [three markers added to
the above: G10M, G10X, and UarMU9;
probability of excluding both parents (PE II = 0.9869)]. 相似文献
207.
Tsutomu Kuboyama Toshiya Saito Takashi Matsumoto Jianzhong Wu Hiroyuki Kanamori Satoru Taura Muneharu Sato Wataru Marubashi Katsuyuki Ichitani 《Rice》2009,2(2-3):93-103
Hybrid weakness is a reproductive barrier. In rice, the hybrid weakness caused by two complementary genes––HWC1 and HWC2––has been surveyed extensively. However, their gene products and the molecular mechanism that causes hybrid weakness have remained unknown. We first performed fine mapping of HWC2, narrowing down the area of interest to 19 kb. We thereby identified five candidate genes. Second, we performed haplotype analysis around the HWC2 locus of 33 cultivars. With 15 DNA markers examined, all the 13 Hwc2-1 carriers share the same haplotype for consecutive 14 DNA markers. As for hwc2-2 carriers, five out of 20 have the haplotypes relatively similar to those of Hwc2-1 carriers. However, the other haplotypes differ remarkably from them. These results are useful to identify the HWC2 gene and to study rice varietal differentiation. 相似文献
208.
Formation of grain chalkiness and changes in water distribution in developing rice caryopses grown under high-temperature stress 总被引:1,自引:0,他引:1
209.
Seiichi Furuya Shunji Suzuki Hironori Kobayashi Seiya Saito Tsutomu Takayanagi 《Pest management science》2009,65(8):840-843
BACKGROUND: The increasing occurrence of Qo inhibitor (QoI)‐fungicide‐resistant Plasmopara viticola (Berk. & MA Curtis) Berl. & DeToni populations is becoming a serious problem in the control of grapevine downy mildew worldwide. RESULTS: The authors have developed a rapid method for detecting resistance to a QoI fungicide, azoxystrobin, in P. viticola populations using the nested PCR‐RFLP method. With this method, a glycine‐to‐alanine substitution was discovered at codon 143 in the cytochrome b gene of P. viticola populations found in Japan. CONCLUSION: It is proposed that the nested PCR‐RFLP method is a high‐speed, sensitive and reliable tool for detecting azoxystrobin‐resistant P. viticola populations. Copyright © 2009 Society of Chemical Industry 相似文献
210.
Spatial distribution analysis of the North Pacific spiny dogfish,Squalus suckleyi,in the North Pacific using generalized additive models
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Toshikazu Yano Seiji Ohshimo Minoru Kanaiwa Tsutomu Hattori Masa‐aki Fukuwaka Toru Nagasawa Sho Tanaka 《Fisheries Oceanography》2017,26(6):668-679
The North Pacific spiny dogfish (SPD), Squalus suckleyi, is a commercially exploited shark species that plays an important role in the ecosystem. To elucidate the distribution of the SPD in the North Pacific and to evaluate the effects of sea surface temperature (SST) and prey availability on its distribution, we estimated the probability of SPD presence using a generalized additive model with a binomial error distribution from SPD presence/absence data on 14,824 operations in fishery‐independent gillnet surveys between 1972 and 2011. The habitat model was structured in the east and west to reflect differences in the North Pacific oceanic environments. In the east, a higher probability of SPD presence was identified along the coast from the eastern Gulf of Alaska to Queen Charlotte Sound. In the west, it was identified around northern Japan. The estimated distribution was continuous between the two areas, whereas the probability of SPD presence was relatively low. Although the probability of SPD presence was higher at SSTs between 6°C and 12°C, the SST at the peak probability of SPD presence differed between the west and east. The prey species, Japanese sardine, Sardinops melanostictus, and walleye pollock, Gadus chalcogrammus, in the west and boreal clubhook squid, Onychoteuthis borealijaponica, in the east significantly affected the probability of SPD presence, which was higher if the prey species co‐existed with SPD. Therefore, SPD might adapt their distribution to that of available prey species. SPD stock assessment and management in these two important areas are required for its sustainable utilization. 相似文献