全文获取类型
收费全文 | 350篇 |
免费 | 9篇 |
国内免费 | 1篇 |
专业分类
林业 | 34篇 |
农学 | 14篇 |
53篇 | |
综合类 | 7篇 |
农作物 | 9篇 |
水产渔业 | 41篇 |
畜牧兽医 | 139篇 |
园艺 | 3篇 |
植物保护 | 60篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 7篇 |
2020年 | 3篇 |
2019年 | 1篇 |
2018年 | 8篇 |
2017年 | 4篇 |
2016年 | 15篇 |
2015年 | 13篇 |
2014年 | 8篇 |
2013年 | 25篇 |
2012年 | 18篇 |
2011年 | 21篇 |
2010年 | 17篇 |
2009年 | 21篇 |
2008年 | 27篇 |
2007年 | 31篇 |
2006年 | 26篇 |
2005年 | 25篇 |
2004年 | 20篇 |
2003年 | 16篇 |
2002年 | 18篇 |
2001年 | 6篇 |
2000年 | 5篇 |
1999年 | 5篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1980年 | 3篇 |
排序方式: 共有360条查询结果,搜索用时 15 毫秒
351.
Kaneshima T Hohdatsu T Satoh K Takano T Motokawa K Koyama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(1):21-25
Canine coronavirus (CCoV) has been reported to cause acute diarrhea mainly in young pups. CCoV and feline coronavirus are classified as group 1 coronaviruses. However, it has recently been reported in the United Kingdom that the group 2 coronavirus gene, which is more closely related to the bovine coronavirus (BCoV) and human coronavirus strain OC43, has been detected in respiratory tract tissue samples from dogs with respiratory disease. In this study, we examined the prevalence of antibodies to group 2 coronaviruses in domestic dogs and cats in Japan by a neutralization test using BCoV. All 104 feline serum samples were negative (<1:5) for anti-BCoV antibodies. In contrast, of the 898 canine serum samples, 160 (17.8%) were positive for anti-BCoV antibodies, and the antibody titers ranged from 1:5 to more than 1:640, with 1:160 being the most frequent. No correlation was found between the titers of the anti-BCoV and anti-CCoV antibodies in the 198 serum samples of dogs with a known history of CCoV vaccination. We amplified, by RT-PCR, group 2 coronavirus-specific hemagglutination/esterase genes in the oral swabs of a total of 10 young pups presenting with or having recovered from respiratory signs, or having anti-BCoV antibodies, with the result that 2 pups were positive for the hemagglutination/esterase genes. These results strongly suggest that an unknown group 2 coronavirus as well as the known enteritis-causing CCoV (group 1 coronavirus) is prevalent among domestic dogs in Japan. 相似文献
352.
Ichihara N Kikuchi N Sawada T Kaseda M Honnma N Shibata S Amasaki H Asari M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(3):201-204
This macroscopic study firstly examined the precise locational information of the canine ciliary body, i.e., the ciliary crown and the ciliary ring in the beagle. The safe and effective transscleral laser photocoagulation technique requires the accurate location of the ciliary body. In both sides of the eyeball in 10 beagle dogs, the width of the ciliary ring and the distance from the limbus to the ciliary ring were measured with calipers using a stereomicroscope at the 8 points. The widest portion of ciliary body was found at the dorsal to ventro-temporal area of the lateral canthus (lateral portion of the eyelid; ear side). In contrast, the narrowest portion was seen at the ventro-nasal to nasal area of the medial canthus (medial portion of the eyelid; nasal quadrants). Use of transscleral photocoagulation at the present narrowest area of ciliary body may carry a high risk of destruction of the optic portion of retina. 相似文献
353.
Kanai Y Inoue T Mano T Nonaka N Katakura K Oku Y 《The Japanese journal of veterinary research》2007,54(4):175-182
In order to evaluate the present epidemiological situation of Trichinella infection in wild animals in Hokkaido, Japan, red foxes (Vulpes vulpes), raccoon dogs (Nyctereutes procyonoides) , brown bears (Ursus arctos) , martens (Martes melampus), rodents and insectivores captured in Hokkaido were examined for muscle larvae by the artificial digestion method from 2000 to 2006. Foxes (44/319, 13.8%), raccoon dogs (6/77, 7.8%) and brown bears (4/126, 3.2%) were found to be infected with Trichinella larvae and all other animal species evaluated were negative. Multiplex PCR and DNA sequencing revealed that larvae from a fox captured in Otofuke, in south-eastern Hokkaido, were T. nativa, and larvae from 27 animals including 21 foxes, 2 raccoon dogs and 4 brown bears captured in western Hokkaido were Trichinella T9. 相似文献
354.
Rosalina M. LAPITAN Arnel N. DEL BARRIO Osamu KATSUBE Tomomi BAN-TOKUDA Edgar A. ORDEN Alberto Y. ROBLES Tsutomu FUJIHARA Libertado C. CRUZ Hideya HOMMA Yukio KANAI 《Animal Science Journal》2007,78(6):596-604
This study was conducted to determine and compare the live traits, carcass yield and the physical, chemical, processing and sensory properties of meat from 20 Brahman grade cattle (crossbred cattle) and crossbred water buffalo (Philippine carabao × Murrah, Bulgarian or Indian) with an average age and weight of 29 months and 434 kg, respectively. The animals were fed a similar diet for 180 days before slaughter. A survey was performed to establish consumer meat preference between the two species. Live weights before slaughter of the two species were found to be different (P < 0.05) with the crossbred water buffalo showing higher bodyweight. However, the crossbred water buffalo had a lower (P < 0.05) dressing yield based on hot carcass weight and chilled carcass weight. The lower dressing yield of crossbred water buffalo can be attributed to its higher (P < 0.05) weights of edible and non‐edible slaughter by‐products. The chilled carcass yield of the forequarters and hindquarters from crossbred cattle and crossbred water buffalo were comparable when expressed as percent of live weight and chilled carcass weight. The estimated lean yield was higher in crossbred cattle than crossbred water buffalo. Crude protein, ash, fat, cholesterol, myofibrillar, sarcoplasmic and insoluble protein contents of the beef and carabao meat were all similar. Water holding capacity, pH, muscle fiber diameter, tenderness, firmness and marbling score in carabeef were all comparable to the beef. Redness was found to be higher (P < 0.05) in carabeef than the beef as measured with a color meter. The consumer meat preference survey showed that 55.9% selected the beef while 44.1% preferred carabeef. The color and amount of fat on the outside of the beef sample were primary in buyer selection. 相似文献
355.
356.
Makrai L Kobayashi A Matsuoka M Sasaki Y Kakuda T Dénes B Hajtós I Révész I Jánosi K Fodor L Varga J Takai S 《Veterinary microbiology》2008,131(3-4):318-323
Rhodococcus equi has been isolated from the submaxillary lymph nodes of domesticated pigs, but little is known about the presence of R. equi in wild boars. The aim of the study was the evaluation of the incidence of R. equi in wild boars and the characterisation of them. Of 482 submaxillary lymph nodes of wild boars shot in 39 settlements throughout Hungary, R. equi was isolated from 60 specimens, and plasmid types of 82 isolates were examined. The isolates were tested for the presence of 15-17-kDa (VapA) and 20-kDa virulence-associated protein antigen (VapB) genes by polymerase chain reaction (PCR). Plasmid DNAs were isolated and analysed by digestion with restriction endonucleases to estimate size and compare their polymorphisms. None of the 82 isolates contained vapA gene but 21 isolates (25.6%) were positive for vapB gene showing 827bp product of the expected size in the PCR amplification. Sixty-one strains (74.4%) did not contain plasmid. The 21 isolates of intermediate virulence contained virulence plasmids that were identified as types 1 (1 isolate), 5 (16 isolates), 21 (1 isolate), and three new distinct plasmid variants (1-1-1 isolate), respectively. On the basis of restriction digestion patterns of plasmid DNAs, we tentatively designated the new variants as types 25-27, respectively. The prevalence of R. equi strains of intermediate virulence among the isolates originated from the submaxillary lymph nodes of wild boars (25.6%) is very similar to those of domestic pigs (26.8%) in Hungary, and plasmid type 5 is the predominating one in both groups. This is the first report of isolation of VapB-positive R. equi from wild boars in the world. 相似文献
357.
358.
Takashi FUJII Nobuyuki SAKURAI Tsubasa OSAKI Gentaro IWAGAMI Hiroki HIRAYAMA Akira MINAMIHASHI Tsutomu HASHIZUME Ken SAWAI 《The Journal of reproduction and development》2013,59(2):151-158
In mouse embryos, segregation of the inner cell mass (ICM) and trophectoderm (TE)
lineages is regulated by genes, such as OCT-4, CDX2 and
TEAD4. However, the molecular mechanisms that regulate the segregation
of the ICM and TE lineages in porcine embryos remain unknown. To obtain insights regarding
the segregation of the ICM and TE lineages in porcine embryos, we examined the mRNA
expression patterns of candidate genes, OCT-4, CDX2,
TEAD4, GATA3, NANOG,
FGF4, FGFR1-IIIc and FGFR2-IIIc, in
blastocyst and elongated stage embryos. In blastocyst embryos, the expression levels of
OCT-4, FGF4 and FGFR1-IIIc were
significantly higher in the ICM than in the TE, while the CDX2,
TEAD4 and GATA3 levels did not differ between the ICM
and TE. The expression ratio of CDX2 to OCT-4
(CDX2/OCT-4) also did not differ between the ICM and
TE at the blastocyst stage. In elongated embryos, OCT-4,
NANOG, FGF4 and FGFR1-IIIc were
abundantly expressed in the embryo disc (ED; ICM lineage), but their expression levels
were very low in the TE. In contrast, the CDX2, TEAD4
and GATA3 levels were significantly higher in the TE than in the ED. In
addition, the CDX2/OCT-4 ratio was markedly higher in
the TE than in the ED. We demonstrated that differences in the expression levels of
OCT-4, CDX2, TEAD4,
GATA3, NANOG, FGF4,
FGFR1-IIIc and FGFR2-IIIc genes between ICM and TE
lineages cells become more clear during development from porcine blastocyst to elongated
embryos, which indicates the possibility that in porcine embryos, functions of ICM and TE
lineage cells depend on these gene expressions proceed as transition from blastocyst to
elongated stage. 相似文献
359.
Xiaoniu Xu Yoshihiro Tokashiki Eiji Hirata Tsutomu Enoki Kangoro Nogami 《Journal of Forest Research》2000,5(3):151-156
Patterns of litterfall and nutrient input in a subtropical evergreen broad-leaved forest in northern Okinawa, Japan, were
studied during May, 1996–April, 1999. The mean annual rate of litterfall in the five sampling plots ranged from 6.84 to 8.93
Mg ha−1 yr−1, of which 63.3–68.5% were leaves; 22.4–29.1% woody parts (including branches < 5.0 cm in diameter and bark); 2.8–5.0% sexual
organs and 4.6–6.3% miscellaneous material. Significant differences were found among plots and among years. Significantly
monthly differences pronounced seasonal patterns in litterfall were observed. Total litterfall and leaf litter showed negative
correlations with relative basal area of the dominant species,Castanopsis sieboldii; and showed positive correlations with mean height of the stands. The dominant species,C. sieboldii produced an average of 2.36 Mg ha−1 yr−1 of leaf litter, which covered 30.5% of the annual litter production, and the nutrient input from those litterfall contributed
32.3, 28.3, 30.2, 22.2, 32.5, and 30.5% of total N, P, K, Ca, Mg, and Na, respectively. Nutrient use efficiency in litter
production was high, especially for P and K compared with other broad-leaved forests in Japan indicating that P and K may
be limiting in Okinawan evergreen broad-leaved forest. 相似文献
360.
The present study was carried out to determine whether leptin or leptin (116–130) peptide amide (lep (116–130)), an active fragment of the native protein in rats, is able to stimulate the release of luteinizing hormone (LH), growth hormone (GH) or prolactin (PRL) from cultured porcine anterior pituitary (AP) cells in vitro. The AP cells were obtained from 6 month‐old pigs and were incubated for 3 h with 10?11?10?7 mol/L leptin or lep (116–130) after being cultured in Dulbecco's modified Eagle's medium for 3–4 days. Leptin significantly increased the concentration of LH and GH in the culture medium at concentrations of 10?8 and 10?7 mol/L, respectively, compared with the controls (P < 0.05). Leptin did not increase the concentration of PRL in the culture medium. In contrast to these results, no effects of lep (116–130) on the release of LH, GH or PRL were seen in the cultured cells. These results suggest that leptin stimulates the release of LH and GH by acting directly on porcine AP cells, and that a fragment of leptin protein comprising amino acids 116–130 is not associated with the secretion of hormones in pigs. 相似文献