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131.
Knudsen T Kjelgaard-Hansen M Tranholm M Wiinberg B Clausen JT Hansen JJ Nichols TC Kjalke M Jensen AL Kristensen AT 《Veterinary journal (London, England : 1997)》2011,190(3):352-358
Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable in the diagnosis of hereditary canine FVII deficiency. 相似文献
132.
Hansen AE Kristensen AT Law I Jørgensen JT Engelholm SA 《Veterinary and comparative oncology》2011,9(1):16-37
Hypoxia-inducible factors (HIFs) play a key role in the cellular response experienced in hypoxic tumours, mediating adaptive responses that allow hypoxic cells to survive in the hostile environment. Identification and understanding of tumour hypoxia and the influence on cellular processes carries important prognostic information and may help identify potential hypoxia circumventing and targeting strategies. This review summarizes current knowledge on HIF regulation and function in tumour cells and discusses the aspects of using companion animals as comparative spontaneous cancer models. Spontaneous tumours in companion animals hold a great research potential for the evaluation and understanding of tumour hypoxia and in the development of hypoxia-targeting therapeutics. 相似文献
133.
Hogerheijde MR Bergin EA Brinch C Cleeves LI Fogel JK Blake GA Dominik C Lis DC Melnick G Neufeld D Panić O Pearson JC Kristensen L Yildiz UA van Dishoeck EF 《Science (New York, N.Y.)》2011,334(6054):338-340
Icy bodies may have delivered the oceans to the early Earth, yet little is known about water in the ice-dominated regions of extrasolar planet-forming disks. The Heterodyne Instrument for the Far-Infrared on board the Herschel Space Observatory has detected emission lines from both spin isomers of cold water vapor from the disk around the young star TW Hydrae. This water vapor likely originates from ice-coated solids near the disk surface, hinting at a water ice reservoir equivalent to several thousand Earth oceans in mass. The water's ortho-to-para ratio falls well below that of solar system comets, suggesting that comets contain heterogeneous ice mixtures collected across the entire solar nebula during the early stages of planetary birth. 相似文献
134.
David M Kristensen Thomas R Jørgensen Rasmus K Larsen Mads C Forchhammer Kirsten S Christoffersen 《BMC ecology》2006,6(1):10-8
Background
Major changes in climate have been observed in the Arctic and climate models predict further amplification of the enhanced greenhouse effect at high-latitudes leading to increased warming. We propose that warming in the Arctic may affect the annual growth conditions of the cold adapted Arctic charr and that such effects can already be detected retrospectrally using otolith data. 相似文献135.
Lisbeth Mogensen Troels Kristensen 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(4):186-196
In Denmark, organic feeding is typically based on homegrown feed with a high proportion of roughage. Which crop to grow and use as supplementary feed is investigated in this paper. In three experiments, barley (B) was compared with another type of supplementary feed: a concentrate mixture (C), grass pellets (G), or fodder beets (F). The concentrate mixture resulted in a higher (P=0.002) milk yield (25.9 vs. 23.7 kg), a tendency of a lower (P=0.07) fat content (4.08 vs. 4.25%), and a higher (P=0.006) ECM yield (25.7 vs. 24.1 kg) compared to feeding barley as supplement at the same energy level. Grass pellets resulted in a lower (P=0.01) milk fat content (3.74 vs. 4.07%), a tendency of a lower (P=0.12) protein content (3.14 vs. 3.23%), but no different milk or ECM yield compared to feeding barley as supplement at the same dry matter level. Fodder beet tended to decrease (P=0.12) the milk yield (20.7 vs. 22.0 kg), and the ECM yield (P=0.05) was lower (21.5 vs. 22.9 kg) whereas the composition of the milk was unaffected compared to the total mixed ration with barley. 相似文献
136.
E. Kristensen S. Østergaard M. A. Krogh C. Enevoldsen 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(2):104-108
Abstract The manager of a dairy herd and the affiliated consultants constantly need to judge whether financial performance of the production system is satisfactory and whether financial performance relates to real (systematic) effects of changes in management. This is no easy task because the dairy herd is a very complex system. Thus, it is difficult to obtain empirical data that allows a valid estimation of the random (within-herd) variation in financial performance corrected for management changes. Thus, simulation seems to be the only option. This study suggests that much caution must be recommended when claming effect of changes in herd management because the link between management changes (cause) and effect (measured as improvement of gross margin per cow year) is extensively blurred by a large within-herd variation in available real life accounting data and differences between herds in time to steady state following management changes. 相似文献
137.
Lisbeth Mogensen Troels Kristensen 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(4):243-252
An experiment was carried out to investigate the effects of barley or rape seed cake as supplement to silage given ad libitum on milk production and health of dairy cows. A total of 103 cows were divided into two groups on two farms. Before the experiment, the cows had an average milk yield of 26.9 kg ECM and they were in milk for an average of 99 days. Their average parity was 2.3 and their weight 596 kg. The cows in each group received either solely barley or an isoenergetic mixture of rape cake seed and barley as supplement. The supplement feed was fed frequently by automatic feeding. A mixture of clover grass silage and whole crop silage was fed ad libitum . The daily milk yields were not significantly different in the barley and rape seed cake treatment (22.8 vs 24.1 kg). The milk fat content was not significant different either (4.51% vs 4.35%). However, the protein content tended to be higher in the barley treatment (3.51% vs 3.45%). But the energy corrected milk yield (ECM) was not significantly different in the barley and rape seed cake treatments either (24.2 vs 24.9 kg ECM). No differences in health as indicated by clinical illness treated by the vet and somatic cell count (SCC) was seen. It was concluded that barley and a mixture of isoenergic rape seed cake and barley had similar feeding value when used as supplement with a high proportion of easily digestible clover grass silage allocated ad libitum and frequently allocation of supplementary feed. 相似文献
138.
Susanne Marie Clausen Jan Tind Sørensen Anders Ringgaard Kristensen 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(1):64-73
Abstract The objective of this study was to create operational replacement guidelines under various conditions concerning reproductive performance, supply of replacement heifers and individual milk yield. Nine culling strategies were defined by three average insemination periods and by three discrimination policies between high- and low-yield cows. The effect of the nine culling strategies was analysed with combinations of heat detection rate and time of initiation of breeding after calving under two replacement heifer purchase policies: purchase (open herd) and no purchase (closed herd). The strategies were evaluated using a stochastic simulation model that simulated production and reproductive status in herds composed of dual-purpose cattle with additional young stock. The evaluation of the strategies was made in a situation without a milk quota under typical Danish conditions in 1993. Results showed that discriminating between high- and low-yield cows improved net revenue significantly in open herds but not in closed herds. Irrespective of the purchase policy, using longer insemination periods increased net revenue significantly in herds with poor reproductive performance; whereas net revenue in herds with good reproductive performance tended to increase by using shorter insemination periods. The culling rate is a poor figure when evaluating culling strategies and culling strategies should be assessed at herd level rather than per cow. 相似文献
139.
Elena R Moldal Thomas Eriksen Jolle Kirpensteijn Ane Nødtvedt Annemarie T Kristensen Fiorella M Sparta H Andreas Haga 《Veterinary anaesthesia and analgesia》2013,40(1):63-73
ObjectiveTo evaluate the usefulness of intratesticular and subcutaneous lidocaine in alleviating the intraoperative nociceptive response to castration, measured by pulse rate (PR) and mean arterial pressure (MAP), and to test the applicability of heart rate variability (HRV) analysis in assessing this response.Study designRandomized, controlled, observer-blinded experimental trial.AnimalsThirty-nine healthy male cats admitted for castration.MethodsOne group received general anaesthesia and served as control group (GA), while the treatment group (LA) additionally received local anaesthesia (lidocaine 2 mg kg?1) intratesticularly and subcutaneously. PR and MAP were recorded at anaesthesia baseline (T0), treatment (T1), incision left testicle (T2), traction on spermatic cord (T3), tightening of the autoligature and resection of the cord (T4), incision on the right side (T5), traction on spermatic cord (T6), and tightening of the autoligature and resection of cord (T7). HRV analysis was divided into three 5-minute intervals: baseline (H0), treatment (H1), and surgery (H2).ResultsThere were significant increases in PR and MAP for both groups during surgery from T3 onwards; however, the increase in the treatment group (LA) was significantly lower than for the control group (GA). For HRV analysis, significant differences were found between groups in the following parameters during surgery: TP (total power), VLF (very low frequency), SDNN (standard deviation of NN intervals [=the interval between two consecutive R-waves in the ECG]), and TI (triangular index), which were lower in the LA group. Mean NN was significantly lower in the GA group, whereas LF (low frequency) and LFn (low frequency, normalized value) were lower in the LA group. HF (high frequency) and HFn (high frequency, normalized value) decreased significantly from H1 to H2 in both groups.Conclusions and clinical relevanceThe study showed that the nociceptive response to surgery was alleviated by the use of intratesticular and subcutaneous lidocaine and that HRV analysis is a promising research tool to estimate intraoperative nociception in cats during general anaesthesia. 相似文献
140.
Gallibacterium anatis is a pathogen in chickens and other avian species where it is a significant cause of salpingitis and peritonitis. We found that bacterial cells and cell-free, filter-sterilised culture supernatant from the haemolytic G. anatis biovar haemolytica were highly cytotoxic towards avian-derived macrophage-like cells (HD11). We obtained the genome sequence of G. anatis 12656-12 and used a rational approach to identify a gene predicted to encode a 2026 amino acid RTX-toxin, which we named GtxA (Gallibacterium toxin). The construction of a gtxA knock-out mutant showed gtxA to be responsible for G. anatis’ haemolytic and leukotoxic activity. In addition, Escherichia coli expressing gtxA and an adjacent acyltransferase, gtxC, became cytolytic. GtxA was expressed during in vitro growth and was localised in the extracellular protein fraction in a growth phase dependent manner. GtxA had an unusual modular structure; the C-terminal 1000 amino acids of GtxA were homologous to the classical pore-forming RTX-toxins in other members of Pasteurellaceae. In contrast, the N-terminal approximately 950 amino acids had few significant matches in sequence databases. Expression of truncated GtxA proteins demonstrated that the C-terminal RTX-domain had a lower haemolytic activity than the full-length toxin, indicating that the N-terminal domain was required for maximal haemolytic activity. Cytotoxicity towards HD11 cells was not detected with the C-terminal alone, suggesting that the N-terminal domain plays a critical role for the leukotoxicity. 相似文献